##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293498
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8020854
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.349917352940224	39.0	39.0	39.0	37.0	39.0
2	40.12011900478428	41.0	40.0	41.0	38.0	41.0
3	40.15577455966659	41.0	40.0	41.0	38.0	41.0
4	40.16915754856029	41.0	40.0	41.0	38.0	41.0
5	40.16762055511795	41.0	40.0	41.0	38.0	41.0
6	40.15410453799558	41.0	40.0	41.0	38.0	41.0
7	40.18013181638763	41.0	40.0	41.0	38.0	41.0
8	40.1591253250589	41.0	40.0	41.0	38.0	41.0
9	40.144402578578294	41.0	40.0	41.0	38.0	41.0
10-14	40.0843956017651	41.0	40.0	41.0	38.0	41.0
15-19	39.96203426717405	41.0	40.0	41.0	38.0	41.0
20-24	39.78481376172662	41.0	40.0	41.0	37.6	41.0
25-29	39.5488493120558	40.2	39.0	41.0	36.6	41.0
30-34	39.35349410424376	40.0	38.8	41.0	35.8	41.0
35-39	39.29217190588434	40.4	38.6	41.0	35.0	41.0
40-44	38.89739905501335	40.0	37.2	41.0	35.0	41.0
45-49	38.28304332680784	39.2	35.6	41.0	35.0	41.0
50-54	37.594922735160125	37.8	35.0	40.6	34.8	41.0
55-59	36.891768881468224	36.4	35.0	39.4	34.0	41.0
60-64	36.089503536655826	35.4	34.8	38.0	33.6	40.2
65-69	35.67521386126714	35.0	35.0	36.8	33.8	39.0
70-74	35.40990006799774	35.0	35.0	36.0	34.0	38.2
75-79	35.1988673026588	35.0	35.0	35.6	34.0	37.0
80-84	35.09465071923763	35.0	35.0	35.0	33.8	36.8
85-89	34.89353627431692	35.0	34.6	35.0	32.6	36.2
90-94	39.62057121455644	40.6	39.8	40.6	37.8	40.6
95-99	39.91716419758127	41.0	40.0	41.0	38.0	41.0
100-104	39.80274927055909	41.0	40.0	41.0	37.2	41.0
105-109	39.655066669721805	41.0	39.6	41.0	36.8	41.0
110-114	39.473187666949	40.6	39.0	41.0	35.8	41.0
115-119	39.1998213954306	40.0	38.4	41.0	35.0	41.0
120-124	38.95884380565594	40.0	38.0	41.0	35.0	41.0
125-129	38.463593229923575	39.6	36.8	40.6	34.6	41.0
130-134	38.22947010282664	39.2	35.8	41.0	34.0	41.0
135-139	37.951090154153285	39.0	35.0	41.0	34.8	41.0
140-144	37.40016382871034	37.2	35.0	40.4	34.2	41.0
145-149	36.70756769915114	36.2	35.0	39.0	34.0	41.0
150-154	36.0366647193288	35.0	35.0	37.4	34.0	39.8
155-159	35.47442697024324	35.0	35.0	36.2	34.0	38.2
160-164	35.06304122048498	35.0	35.0	35.6	34.0	36.8
165-169	34.79751497465356	35.0	35.0	35.0	33.2	36.0
170-174	34.655896954522824	35.0	35.0	35.0	33.0	36.0
175-178	34.340524190144016	35.0	34.5	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	3.0
29	46.0
30	509.0
31	3526.0
32	17393.0
33	63383.0
34	175532.0
35	424646.0
36	1101074.0
37	2338222.0
38	2899716.0
39	983177.0
40	13626.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.73810918388491	27.798324218343833	28.309416927424436	22.154149670346822
2	22.028389994382145	28.25246538585542	28.763957054947014	20.955187564815418
3	20.68010463723688	27.439148000948528	29.68994324045794	22.190804121356653
4	21.326045830032562	26.914515586494904	29.807960598709315	21.95147798476322
5	21.877071942713332	27.506771722811564	28.580348177388593	22.035808157086514
6	22.130311809690088	27.445917853635038	28.176912333773934	22.246858002900936
7	22.187288286259793	27.857669519978796	27.81235015623025	22.142692037531166
8	22.03887516217101	28.081498553645286	27.961237045332084	21.918389238851624
9	22.33404323280289	27.872979610400588	27.71677429859713	22.07620285819939
10-14	22.160149031512105	27.965311424444327	27.79102823714283	22.083511306900736
15-19	22.195279953979963	27.885768772252927	27.80519630453316	22.113754969233952
20-24	22.098539631814766	27.82999166921627	27.92904097244508	22.142427726523884
25-29	22.09040832809075	27.894119503982996	27.798336685844177	22.21713548208208
30-34	22.134296422799864	27.9067041988297	27.799772941883745	22.159226436486687
35-39	22.088772592045686	27.909928294418524	27.860210396548794	22.141088716986996
40-44	22.11360037222969	27.94156332979007	27.82083304346395	22.12400325451629
45-49	22.27146136807876	27.83015873372087	27.70470575826464	22.193674139935723
50-54	22.188751970800116	27.92695640638765	27.778209153289662	22.106082469522573
55-59	22.16442289062985	27.89224439193133	27.761108729818545	22.18222398762027
60-64	22.179388878042165	27.889750891862636	27.767020818481424	22.163839411613775
65-69	22.10285588043368	27.88808772731682	27.826560613121742	22.18249577912776
70-74	22.08271837387889	27.952085900079965	27.798740632855306	22.16645509318584
75-79	22.128888021150864	27.867695883755022	27.788746684579973	22.214669410514144
80-84	22.06717888145078	27.913671038021636	27.821471379481537	22.197678701046048
85-89	22.062760399329047	27.92307652028076	27.774496331687377	22.23966674870282
90-94	21.521580727427857	27.57421069193385	28.936073631694192	21.968134948944105
95-99	22.08237666694581	27.88983845884368	27.893996892157674	22.13378798205284
100-104	22.082413428810657	27.93975977739146	27.86586158974451	22.111965204053373
105-109	21.9984219122542	27.97622410599811	27.935833618350053	22.089520363397636
110-114	22.05924634819646	27.795772671612344	27.93026297116934	22.214718009021862
115-119	22.039369078471456	27.885322156347915	27.85977662018761	22.215532144993023
120-124	21.97514952455354	27.943700402803316	27.897603454949405	22.183546617693732
125-129	21.989758129972643	27.899405946919515	27.909050045598377	22.20178587750947
130-134	22.034747951771248	27.957809844003478	27.862991690462767	22.144450513762507
135-139	22.087005548848218	27.928535949736165	27.82241677664065	22.162041724774966
140-144	22.112144099711593	27.97044468933157	27.833755467428993	22.083655743527846
145-149	22.011231896441306	27.994852989270402	27.922391254711627	22.071523859576672
150-154	21.95054847674381	28.120497530011463	27.93696423439982	21.991989758844912
155-159	21.93573434214583	28.081178462297622	27.954538612350678	22.028548583205865
160-164	21.84823262895201	28.208073183982705	28.025595004063174	21.918099183002106
165-169	21.77553503375075	28.239423623833304	28.07307638189827	21.911964960517672
170-174	21.782390690468706	28.31946969931472	28.071059277057703	21.82708033315887
175-178	21.679280695570785	28.41101497379287	28.106388961397432	21.80331536923891
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	3.0
11	7.0
12	20.0
13	45.0
14	100.5
15	238.0
16	525.0
17	1070.0
18	2087.0
19	3854.0
20	6573.0
21	10545.0
22	16124.0
23	23357.0
24	32184.5
25	43115.0
26	55556.5
27	69026.0
28	83349.0
29	99234.5
30	117199.5
31	135813.5
32	155166.0
33	176235.5
34	198231.0
35	220085.5
36	241660.5
37	262635.5
38	282475.0
39	299636.5
40	313275.5
41	324331.0
42	330229.5
43	331401.5
44	329689.0
45	322615.5
46	311233.5
47	296763.5
48	279892.0
49	261265.0
50	241903.5
51	222854.0
52	204144.5
53	186222.5
54	169932.5
55	155774.5
56	142610.0
57	130620.0
58	120262.5
59	110959.0
60	101986.5
61	93310.0
62	84586.0
63	75776.0
64	67332.0
65	58576.0
66	49666.0
67	41174.0
68	33294.5
69	26315.0
70	20307.0
71	15015.5
72	10721.5
73	7493.5
74	5131.0
75	3421.5
76	2165.0
77	1352.5
78	796.0
79	426.5
80	246.0
81	139.0
82	69.5
83	28.5
84	9.0
85	5.5
86	3.0
87	1.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	821.0
92-93	2027.0
94-95	3130.0
96-97	4286.0
98-99	4702.0
100-101	5568.0
102-103	6135.0
104-105	7341.0
106-107	8222.0
108-109	9691.0
110-111	10389.0
112-113	10362.0
114-115	12001.0
116-117	12352.0
118-119	12059.0
120-121	12099.0
122-123	12919.0
124-125	16053.0
126-127	13868.0
128-129	14449.0
130-131	14736.0
132-133	17638.0
134-135	78629.0
136-137	82812.0
138-139	73543.0
140-141	69825.0
142-143	68399.0
144-145	72581.0
146-147	74744.0
148-149	80400.0
150-151	78922.0
152-153	89420.0
154-155	69596.0
156-157	69076.0
158-159	95938.0
160-161	73006.0
162-163	73651.0
164-165	77039.0
166-167	78903.0
168-169	83845.0
170-171	79114.0
172-173	87205.0
174-175	89530.0
176-177	240837.0
178-179	5922991.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.31011438686696
#Duplication Level	Percentage of total
1	91.04660158290616
2	7.824779864760613
3	0.88746359190188
4	0.172086254348264
5	0.040984749087675895
6	0.014434024042707423
7	0.00726187578165088
8	0.003131538143038075
9	0.0
>10	0.0032565190280024873
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.233750171739817E-5	0.0	1.2467500343479635E-5	2.493500068695927E-5	1.2467500343479635E-5	0.0
2	0.0010971400302262077	0.0	1.2467500343479635E-5	3.7402501030438904E-5	1.2467500343479635E-5	0.0
3	0.0010971400302262077	1.2467500343479635E-5	1.2467500343479635E-5	6.233750171739817E-5	1.2467500343479635E-5	0.0
4	0.0010971400302262077	1.2467500343479635E-5	1.2467500343479635E-5	6.233750171739817E-5	1.2467500343479635E-5	0.0
5	0.0011096075305696874	3.7402501030438904E-5	1.2467500343479635E-5	7.480500206087781E-5	1.2467500343479635E-5	0.0
6	0.0011096075305696874	3.7402501030438904E-5	3.7402501030438904E-5	8.727250240435744E-5	1.2467500343479635E-5	0.0
7	0.0011220750309131671	3.7402501030438904E-5	3.7402501030438904E-5	8.727250240435744E-5	1.2467500343479635E-5	0.0
8	0.0011220750309131671	3.7402501030438904E-5	4.987000137391854E-5	8.727250240435744E-5	1.2467500343479635E-5	0.0
9	0.0011220750309131671	3.7402501030438904E-5	4.987000137391854E-5	9.974000274783708E-5	1.2467500343479635E-5	0.0
10-14	0.0011519970317375183	6.233750171739817E-5	4.987000137391854E-5	1.4462300398436376E-4	2.493500068695927E-5	0.0
15-19	0.001199373533042741	9.475300261044523E-5	5.236350144261447E-5	2.0945400577045787E-4	3.7402501030438904E-5	0.0
20-24	0.0012093475333175246	1.2467500343479635E-4	8.22855022669656E-5	2.4935000686959275E-4	8.477900233566151E-5	0.0
25-29	0.0012268020337983959	1.4212950391566783E-4	1.097140030226208E-4	2.9173950803742344E-4	1.1220750309131671E-4	0.0
30-34	0.0012966200357218821	1.4961000412175562E-4	1.1220750309131671E-4	3.39116009342646E-4	1.396360038469719E-4	0.0
35-39	0.001503580541423644	1.5459700425914748E-4	1.1220750309131671E-4	3.665445100983013E-4	1.870125051521945E-4	0.0
40-44	0.001737969547881061	1.6207750446523524E-4	1.4462300398436376E-4	4.214015116096117E-4	2.169345059765456E-4	0.0
45-49	0.00197734555447587	1.7454500480871489E-4	1.69558004671323E-4	4.637910127774424E-4	2.4685650680089676E-4	2.493500068695927E-6
50-54	0.002284046062925469	1.8202550501480265E-4	1.944930053582823E-4	5.13661014151361E-4	2.6431100728176827E-4	1.2467500343479635E-5
55-59	0.0025707985708255006	1.9948000549567415E-4	2.0696050570176194E-4	5.410895149070161E-4	2.9672650817481525E-4	1.7454500480871487E-5
60-64	0.0027004605743976887	2.1942800604524154E-4	2.3688250652611305E-4	6.059205166931102E-4	3.2415500893047053E-4	3.7402501030438904E-5
65-69	0.0028226420777637895	2.4186950666350493E-4	2.593240071443764E-4	6.50803517929637E-4	3.515835096861257E-4	4.737650130522261E-5
70-74	0.004398534121179615	2.917395080374235E-4	3.0171350831220716E-4	7.15634519715731E-4	4.3137551188439534E-4	4.987000137391854E-5
75-79	0.032906720406580145	3.515835096861257E-4	3.1916800879307867E-4	7.330890201966026E-4	4.7875201318961795E-4	5.9844001648702244E-5
80-84	0.044765806733297976	3.864925106478687E-4	3.241550089304705E-4	8.078940222574803E-4	6.034270166244143E-4	6.233750171739817E-5
85-89	0.044860559735908426	4.3137551188439534E-4	3.341290092052542E-4	8.452965232879191E-4	8.752185241122703E-4	6.233750171739817E-5
90-94	0.0449328712379006	4.3636251202178715E-4	3.665445100983013E-4	9.001535247992295E-4	8.85192524387054E-4	6.233750171739817E-5
95-99	0.0450026892398241	4.637910127774424E-4	4.0644051119743615E-4	9.89919527272283E-4	8.85192524387054E-4	6.233750171739817E-5
100-104	0.04506253324147279	4.962065136704895E-4	4.1890801154091575E-4	0.0010672180294018566	8.85192524387054E-4	6.233750171739817E-5
105-109	0.04512985774332758	5.161545142200568E-4	4.3137551188439534E-4	0.0010996335302949037	8.926730245931418E-4	6.233750171739817E-5
110-114	0.04521463674566324	5.361025147696243E-4	4.463365122965709E-4	0.0011270620310505591	8.976600247305337E-4	6.233750171739817E-5
115-119	0.04531936374854847	5.48570015113104E-4	4.4883001236526685E-4	0.0011769320324244777	9.051405249366213E-4	6.233750171739817E-5
120-124	0.04556372675528068	5.735050158000632E-4	4.637910127774424E-4	0.0012218150336610041	9.500235261731482E-4	6.233750171739817E-5
125-129	0.04593775176558506	5.984400164870225E-4	4.8124551325831393E-4	0.0012567240346227472	9.774520269288036E-4	6.233750171739817E-5
130-134	0.04628933527527118	6.183880170365899E-4	4.987000137391854E-4	0.0012941265356531861	0.0010048805276844587	6.233750171739817E-5
135-139	0.046620970784407745	6.283620173113736E-4	5.086740140139691E-4	0.001363944537576672	0.0010248285282340262	6.233750171739817E-5
140-144	0.04696507379388778	6.582840181357248E-4	5.410895149070161E-4	0.00143874953963755	0.0010497635289209851	6.233750171739817E-5
145-149	0.047361540304810434	6.707515184792044E-4	5.535570152504957E-4	0.0014786455407366848	0.0010871660299514242	6.233750171739817E-5
150-154	0.04773307181504614	6.8571251889138E-4	5.80985516006151E-4	0.0014936065411488603	0.0011170880307757752	6.233750171739817E-5
155-159	0.048012343822740076	6.931930190974677E-4	5.984400164870225E-4	0.0015110610416297315	0.0011220750309131671	6.233750171739817E-5
160-164	0.04831904433118967	7.231150199218188E-4	6.258685172426776E-4	0.0015235285419732112	0.0011395295313940386	6.233750171739817E-5
165-167	0.0486897446747358	7.480500206087781E-4	6.358425175174614E-4	0.0015376583756958216	0.0011470100316001263	6.233750171739817E-5
>>END_MODULE
