##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293499
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8068559
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.306061466489865	39.0	39.0	39.0	37.0	39.0
2	40.09504373705391	41.0	40.0	41.0	38.0	41.0
3	40.141492923333644	41.0	40.0	41.0	38.0	41.0
4	40.163683254965356	41.0	40.0	41.0	38.0	41.0
5	40.16992650112616	41.0	40.0	41.0	38.0	41.0
6	40.14668381305757	41.0	40.0	41.0	38.0	41.0
7	40.1735192120427	41.0	40.0	41.0	38.0	41.0
8	40.16069907898052	41.0	40.0	41.0	38.0	41.0
9	40.14401766164194	41.0	40.0	41.0	38.0	41.0
10-14	40.08415165582851	41.0	40.0	41.0	38.0	41.0
15-19	39.96606201925275	41.0	40.0	41.0	38.0	41.0
20-24	39.783344014711915	41.0	40.0	41.0	37.6	41.0
25-29	39.54753296096614	40.2	39.0	41.0	36.6	41.0
30-34	39.353902598964694	40.0	38.8	41.0	35.8	41.0
35-39	39.28410852545046	40.4	38.4	41.0	35.0	41.0
40-44	38.88439400393552	40.0	37.2	41.0	35.0	41.0
45-49	38.26905783300339	39.2	35.6	41.0	35.0	41.0
50-54	37.597709008510684	37.8	35.0	40.6	34.8	41.0
55-59	36.90141089629511	36.4	35.0	39.4	34.0	41.0
60-64	36.119194889694676	35.4	34.8	38.0	33.6	40.2
65-69	35.70321486153847	35.0	35.0	36.8	33.8	39.0
70-74	35.43379079213525	35.0	35.0	36.0	34.0	38.2
75-79	35.21784425199097	35.0	35.0	35.6	34.0	37.0
80-84	35.12583840559386	35.0	35.0	35.0	33.8	37.0
85-89	34.9359675996668	35.0	34.6	35.0	32.6	37.2
90-94	39.65542722332321	40.6	39.8	40.6	37.8	40.6
95-99	39.93008606537526	41.0	40.0	41.0	38.0	41.0
100-104	39.81098685700588	41.0	40.0	41.0	37.2	41.0
105-109	39.66594929260101	41.0	39.8	41.0	36.8	41.0
110-114	39.48227533446578	40.6	39.0	41.0	35.8	41.0
115-119	39.208341479710896	40.0	38.4	41.0	35.0	41.0
120-124	38.964013670810246	40.0	38.0	41.0	35.0	41.0
125-129	38.47009420069607	39.6	36.8	40.6	34.6	41.0
130-134	38.23276426617902	39.2	35.8	41.0	34.2	41.0
135-139	37.953972055300724	39.0	35.0	41.0	34.8	41.0
140-144	37.40302109316006	37.2	35.0	40.4	34.4	41.0
145-149	36.70346944760276	36.2	35.0	39.0	34.0	41.0
150-154	36.036330618018184	35.0	35.0	37.2	34.0	39.8
155-159	35.47444704227891	35.0	35.0	36.2	34.0	38.2
160-164	35.06465452960171	35.0	35.0	35.6	34.0	36.8
165-169	34.799743752837905	35.0	35.0	35.0	33.2	36.0
170-174	34.65722105805382	35.0	35.0	35.0	33.0	36.0
175-178	34.33457735016607	35.0	34.5	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	44.0
30	452.0
31	3392.0
32	17073.0
33	62771.0
34	172633.0
35	421395.0
36	1102105.0
37	2353382.0
38	2929278.0
39	991803.0
40	14231.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.74777429278264	27.801507059686863	28.31311018485457	22.137608462675924
2	22.023994619113523	28.221532494216127	28.773291488604098	20.981181398066248
3	20.6777938910777	27.449808571766038	29.66239696580269	22.21000057135357
4	21.349574316801796	26.888208910661742	29.786595103289198	21.975621669247257
5	21.87621110535351	27.50225907748831	28.568583312088318	22.052946505069865
6	22.151415141167092	27.46652779015435	28.150354976644525	22.23170209203403
7	22.205476839172896	27.83477198344835	27.777252418926352	22.182498758452407
8	22.053702526064445	28.065085723485446	27.939672994892895	21.941538755557218
9	22.328187722243836	27.9061106202483	27.69575087695337	22.069950780554496
10-14	22.169321684330498	27.956759069370378	27.778704970738886	22.09521427556023
15-19	22.211924087064368	27.880604207021353	27.793208180048012	22.114263525866267
20-24	22.10103687659717	27.82995327914191	27.919520697561985	22.149489146698933
25-29	22.089530484935413	27.892730288022932	27.791958886338936	22.22578034070272
30-34	22.149251185992444	27.90962797694111	27.78132997478236	22.159790862284083
35-39	22.089979140017444	27.904291212346592	27.848618322057263	22.157111325578708
40-44	22.125080822982145	27.951449571106814	27.806645523692648	22.116824082218397
45-49	22.26928253235801	27.827511703143028	27.70528913527186	22.1979166292271
50-54	22.19714325693101	27.898696656986704	27.776117148055807	22.12804293802648
55-59	22.16596296810868	27.883328361359194	27.750176952290985	22.200531718241137
60-64	22.187971854701686	27.886644938705906	27.76761253155613	22.15777067503627
65-69	22.11128405952042	27.892544381220986	27.81435941659471	22.181812142663887
70-74	22.0833534215961	27.93926647868597	27.803366127706326	22.174013972011608
75-79	22.12855851955721	27.85173412005787	27.78486220401933	22.23484515636559
80-84	22.086694786516404	27.907942421936806	27.802466338784903	22.202896452761888
85-89	22.069207153346714	27.91258513447073	27.77500170724413	22.24320600493843
90-94	21.53916893852875	27.5628342188319	28.92330372437954	21.97469311825981
95-99	22.099254386950886	27.8800345530056	27.875203543792143	22.14550751625137
100-104	22.09044009503225	27.933406444495617	27.862843552335608	22.113309908136518
105-109	22.029829910908205	27.959109492039747	27.91347721234296	22.097583384709086
110-114	22.077459023376292	27.786255769799318	27.91449157538474	22.221793631439652
115-119	22.04359840180693	27.884858057489897	27.84213625364853	22.22940728705465
120-124	21.997419084031513	27.917171118805427	27.889436841417865	22.195972955745194
125-129	22.009337329388153	27.898137645974263	27.88298655158438	22.2095384730532
130-134	22.04669323782495	27.934300828002733	27.858297400867098	22.16070853330522
135-139	22.09861883002339	27.92699103311051	27.816746374533487	22.15764376233261
140-144	22.11039400939522	27.94792412097531	27.842920518821334	22.098761350808136
145-149	22.04067394296321	27.971058079727058	27.894581466400254	22.093686510909478
150-154	21.97903792355525	28.0866878137902	27.938326671247673	21.99594759140688
155-159	21.952955629167604	28.069053073252263	27.966168294213194	22.01182300336694
160-164	21.871629677070132	28.197790027931617	28.020165769511056	21.910414525487194
165-169	21.779555915686114	28.22624529858036	28.064525480948504	21.929673304785023
170-174	21.80650360149714	28.303060162225446	28.057562568896365	21.832873667381048
175-178	21.693043052053316	28.382372753542874	28.10418560059313	21.820398593810687
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	4.0
11	11.0
12	20.5
13	55.0
14	125.0
15	262.0
16	569.0
17	1131.5
18	2108.0
19	3831.0
20	6536.5
21	10642.0
22	16179.5
23	23448.0
24	32473.5
25	43027.0
26	55094.0
27	68578.5
28	83740.5
29	100385.0
30	117556.0
31	136110.0
32	156390.0
33	177280.5
34	198953.0
35	221156.0
36	243239.5
37	264464.0
38	284223.0
39	301121.5
40	315136.0
41	325755.0
42	331947.0
43	333880.0
44	330547.0
45	323157.0
46	312470.0
47	298365.0
48	281417.0
49	262793.0
50	243709.5
51	224635.0
52	205888.0
53	187838.5
54	171179.0
55	156557.5
56	143594.5
57	131463.0
58	121311.0
59	112500.0
60	103167.5
61	94019.0
62	85666.5
63	76782.5
64	67838.5
65	59231.5
66	50358.5
67	41496.0
68	33488.5
69	26539.0
70	20413.0
71	15183.5
72	10731.5
73	7482.5
74	5147.0
75	3379.0
76	2210.0
77	1375.0
78	797.0
79	471.5
80	275.5
81	156.0
82	76.0
83	28.5
84	15.5
85	8.0
86	4.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	962.0
92-93	2451.0
94-95	2719.0
96-97	3745.0
98-99	5741.0
100-101	5338.0
102-103	6445.0
104-105	7582.0
106-107	8225.0
108-109	9035.0
110-111	10384.0
112-113	11300.0
114-115	11076.0
116-117	11657.0
118-119	12093.0
120-121	12025.0
122-123	12865.0
124-125	13053.0
126-127	13657.0
128-129	14334.0
130-131	15429.0
132-133	17232.0
134-135	84600.0
136-137	76195.0
138-139	71170.0
140-141	74830.0
142-143	69934.0
144-145	68806.0
146-147	93178.0
148-149	78028.0
150-151	72510.0
152-153	73126.0
154-155	77490.0
156-157	69947.0
158-159	72827.0
160-161	75630.0
162-163	73344.0
164-165	77689.0
166-167	82699.0
168-169	86799.0
170-171	82321.0
172-173	87140.0
174-175	99383.0
176-177	254576.0
178-179	5958989.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.28852721956979
#Duplication Level	Percentage of total
1	90.98303931893841
2	7.945191033254037
3	0.8428790694231626
4	0.1537409486363157
5	0.04507668627320632
6	0.017523840007480068
7	0.006223103399054485
8	0.002087158965225631
9	0.002100001720733037
>10	0.002138839382376092
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.9575147185513545E-5	0.0	1.2393786796378386E-5	2.4787573592756773E-5	0.0	0.0
2	0.0012269848928414602	0.0	3.718136038913516E-5	3.718136038913516E-5	0.0	0.0
3	0.0012269848928414602	3.718136038913516E-5	4.9575147185513545E-5	3.718136038913516E-5	0.0	0.0
4	0.0012269848928414602	3.718136038913516E-5	4.9575147185513545E-5	3.718136038913516E-5	0.0	0.0
5	0.0012269848928414602	3.718136038913516E-5	4.9575147185513545E-5	3.718136038913516E-5	0.0	0.0
6	0.0012269848928414602	3.718136038913516E-5	4.9575147185513545E-5	4.9575147185513545E-5	0.0	0.0
7	0.0012269848928414602	4.9575147185513545E-5	4.9575147185513545E-5	4.9575147185513545E-5	0.0	0.0
8	0.0012269848928414602	4.9575147185513545E-5	4.9575147185513545E-5	6.196893398189194E-5	0.0	0.0
9	0.0012393786796378386	4.9575147185513545E-5	4.9575147185513545E-5	6.196893398189194E-5	2.4787573592756773E-5	0.0
10-14	0.0012393786796378386	4.9575147185513545E-5	4.9575147185513545E-5	1.0410780908957844E-4	3.470260302985948E-5	0.0
15-19	0.0012418574369971141	4.9575147185513545E-5	7.188396341899464E-5	1.4872544155654065E-4	3.718136038913516E-5	0.0
20-24	0.001259208738512044	6.196893398189194E-5	9.915029437102709E-5	1.6111922835291903E-4	3.718136038913516E-5	0.0
25-29	0.001274081282667698	1.115440811674055E-4	1.3137414004161092E-4	2.1813064761625958E-4	4.213887510768652E-5	0.0
30-34	0.0014004979079907576	1.2889538268233522E-4	1.3881041211943792E-4	2.528332506461191E-4	6.692644870044329E-5	0.0
35-39	0.0017177788499780442	1.5616171363436768E-4	1.4872544155654065E-4	3.0488715519090824E-4	1.3385289740088657E-4	7.436272077827031E-6
40-44	0.0019631758285463363	1.7847052986784875E-4	1.6111922835291903E-4	3.4454727293931916E-4	1.8838555930495148E-4	1.2393786796378386E-5
45-49	0.002307723101485655	2.082156181791569E-4	1.9582183138277853E-4	4.089949642804867E-4	2.602695227239461E-4	1.2393786796378386E-5
50-54	0.0025308112638204663	2.2804567705336228E-4	2.1069437553843258E-4	4.511338393881732E-4	3.0488715519090824E-4	2.7266330952032448E-5
55-59	0.002773729485029483	2.3796070649046499E-4	2.2060940497553526E-4	4.907939571365841E-4	3.371110008614921E-4	4.4617632466962195E-5
60-64	0.0030240839783163263	2.553120080053948E-4	2.4043946384974071E-4	5.180602880886166E-4	3.7429236125062726E-4	5.205390454478922E-5
65-69	0.0032223845670583803	2.6026952272394614E-4	2.949721257538056E-4	5.45326619040649E-4	3.9907993484338407E-4	6.196893398189194E-5
70-74	0.004736905313575819	2.6026952272394614E-4	3.14802184628011E-4	5.800292220705085E-4	4.5609135410672457E-4	6.196893398189194E-5
75-79	0.032816268679450686	2.8009958159815155E-4	3.2223845670583805E-4	6.469556707709519E-4	5.031877439329625E-4	6.196893398189194E-5
80-84	0.04488533826176396	3.1728094198728676E-4	3.495047876578705E-4	6.965308179564653E-4	5.849867367890599E-4	6.196893398189194E-5
85-89	0.04496713725462006	3.4454727293931916E-4	3.718136038913516E-4	7.337121783456005E-4	8.204686859202493E-4	6.196893398189194E-5
90-94	0.045036542460679785	3.470260302985948E-4	3.8420739068773E-4	7.832873255311141E-4	8.427775021537303E-4	6.196893398189194E-5
95-99	0.04507868133578748	3.5694105973569756E-4	3.9660117748410836E-4	8.427775021537303E-4	8.52692531590833E-4	6.196893398189194E-5
100-104	0.04513321399769153	3.6685608917280026E-4	4.0651620692121106E-4	8.92352649339244E-4	8.6012880366866E-4	6.196893398189194E-5
105-109	0.045172874115439944	3.9164366276555707E-4	4.263462657954165E-4	9.419277965247575E-4	8.700438331057627E-4	6.196893398189194E-5
110-114	0.04527202440981097	3.9660117748410836E-4	4.486550820288975E-4	9.939817010695465E-4	8.973101640577952E-4	6.196893398189194E-5
115-119	0.04530672701284083	4.040374495619354E-4	4.833576850587571E-4	0.0010485143629736116	9.29534009728379E-4	6.196893398189194E-5
120-124	0.04547032499855303	4.486550820288975E-4	5.106240160107896E-4	0.001098089510159125	9.518428259618602E-4	6.196893398189194E-5
125-129	0.04589915002170772	4.660063835438273E-4	5.329328322442706E-4	0.0011625372015002925	9.815879142731684E-4	6.196893398189194E-5
130-134	0.04631806001542531	4.8087892769948143E-4	5.45326619040649E-4	0.0012021973192487035	0.001006375487865925	6.196893398189194E-5
135-139	0.0467766301268913	5.106240160107896E-4	5.552416484777518E-4	0.001259208738512044	0.0010410780908957846	6.196893398189194E-5
140-144	0.04727981787082427	5.527628911184761E-4	5.577204058370274E-4	0.0013087838856975576	0.0010633869071292657	6.196893398189194E-5
145-149	0.04757974751129662	5.849867367890598E-4	5.825079794297841E-4	0.00135340151816452	0.0011203983263926062	6.444769134116761E-5
150-154	0.04787471963705043	5.973805235854382E-4	6.097743103818166E-4	0.001390582878553655	0.0011278345984704332	7.436272077827032E-5
155-159	0.04827627832925309	6.271256118967463E-4	6.419981560524003E-4	0.0014203279668649632	0.0011402283852668116	7.436272077827032E-5
160-164	0.04866792199201865	6.320831266152978E-4	6.444769134116761E-4	0.0014575093272540982	0.0011724522309373953	8.179899285609735E-5
165-167	0.049137233385374846	6.320831266152978E-4	6.444769134116761E-4	0.0014665981042381092	0.0011939347947177846	8.67565075746487E-5
>>END_MODULE
