##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293500
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8075445
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3435821803009	39.0	39.0	39.0	37.0	39.0
2	40.110578054831656	41.0	40.0	41.0	38.0	41.0
3	40.15142100528206	41.0	40.0	41.0	38.0	41.0
4	40.16481717106611	41.0	40.0	41.0	38.0	41.0
5	40.156726966749204	41.0	40.0	41.0	38.0	41.0
6	40.149280937459174	41.0	40.0	41.0	38.0	41.0
7	40.175198023143	41.0	40.0	41.0	38.0	41.0
8	40.15876190104694	41.0	40.0	41.0	38.0	41.0
9	40.14298741431587	41.0	40.0	41.0	38.0	41.0
10-14	40.07962518474214	41.0	40.0	41.0	38.0	41.0
15-19	39.96246064458367	41.0	40.0	41.0	38.0	41.0
20-24	39.78949212086764	41.0	40.0	41.0	37.6	41.0
25-29	39.547214624085726	40.4	39.0	41.0	36.6	41.0
30-34	39.34158583211204	40.0	38.8	41.0	35.8	41.0
35-39	39.2789035650667	40.4	38.6	41.0	35.0	41.0
40-44	38.88760208260969	40.0	37.2	41.0	35.0	41.0
45-49	38.28105170674805	39.2	35.6	41.0	35.0	41.0
50-54	37.59820586976941	37.8	35.0	40.6	34.8	41.0
55-59	36.90662619830858	36.4	35.0	39.4	34.0	41.0
60-64	36.119502590878895	35.4	34.8	38.0	33.6	40.2
65-69	35.70831809268715	35.0	35.0	36.8	33.8	39.0
70-74	35.43167520799163	35.0	35.0	36.0	34.0	38.2
75-79	35.210049279018065	35.0	35.0	35.6	34.0	37.0
80-84	35.11271331796576	35.0	35.0	35.0	33.8	37.0
85-89	34.91515335687384	35.0	34.6	35.0	32.6	37.2
90-94	39.61905400643008	40.6	39.8	40.6	37.8	40.6
95-99	39.90593035783563	41.0	40.0	41.0	38.0	41.0
100-104	39.79300073585649	41.0	40.0	41.0	37.0	41.0
105-109	39.645190514182346	41.0	39.6	41.0	36.6	41.0
110-114	39.463801564100535	40.6	39.0	41.0	35.8	41.0
115-119	39.193150730771535	40.0	38.4	41.0	35.0	41.0
120-124	38.946698284811724	40.0	38.0	41.0	35.0	41.0
125-129	38.45599314360441	39.6	36.8	40.6	34.6	41.0
130-134	38.224300640290586	39.2	35.8	41.0	34.0	41.0
135-139	37.952522485057116	39.0	35.0	41.0	34.8	41.0
140-144	37.40311266362372	37.2	35.0	40.4	34.2	41.0
145-149	36.71236991034503	36.2	35.0	39.0	34.0	41.0
150-154	36.043229117304996	35.0	35.0	37.4	34.0	39.8
155-159	35.478794068344335	35.0	35.0	36.2	34.0	38.2
160-164	35.064470177722455	35.0	35.0	35.6	34.0	36.8
165-169	34.806726391432406	35.0	35.0	35.0	33.2	36.0
170-174	34.65872976770173	35.0	35.0	35.0	33.0	36.0
175-178	34.346633949244236	35.0	34.5	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	41.0
30	498.0
31	3483.0
32	17519.0
33	63168.0
34	176255.0
35	428122.0
36	1106158.0
37	2352579.0
38	2918015.0
39	995067.0
40	14539.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.744584973335833	27.818665101427847	28.32297167524514	22.113778249991174
2	21.998998197622548	28.26594695400687	28.77473625292476	20.960318595445823
3	20.68750390845334	27.47016418290261	29.66413615596416	22.178195752679883
4	21.324991997345037	26.897118363136645	29.787720676693358	21.99016896282496
5	21.874831665623383	27.499363812149053	28.60070249998607	22.025102022241498
6	22.110360481682434	27.476417708250132	28.20253249201747	22.210689318049965
7	22.176239204155312	27.8678140956938	27.815073968059966	22.140872732090923
8	22.01798167159828	28.088000104019034	27.956775137469204	21.937243086913476
9	22.30225083571246	27.88794920899096	27.749182862368578	22.060617092928005
10-14	22.14313390779084	27.96936639405011	27.81049960714239	22.077000091016654
15-19	22.19666903805301	27.895763019870735	27.811413488668425	22.09615445340783
20-24	22.082505669966178	27.840246574646972	27.94315607375197	22.13409168163488
25-29	22.08492782750672	27.883880083388597	27.813057980086548	22.218134109018138
30-34	22.12417024696472	27.91198008283135	27.807076885546245	22.15677278465769
35-39	22.073493163534643	27.931359819799408	27.866191894068997	22.128955122596956
40-44	22.0937471557295	27.97268757325448	27.82728629815447	22.106278972861556
45-49	22.25151926612094	27.84418939142054	27.729572797536235	22.174718544922293
50-54	22.169262498846813	27.919271321889997	27.8011651370296	22.110301042233584
55-59	22.143114094641227	27.91589070323679	27.780913621478444	22.160081580643542
60-64	22.162833131796454	27.90278678140957	27.779115578150805	22.155264508643178
65-69	22.092100187667675	27.89399469626751	27.853816105490164	22.160089010574648
70-74	22.070332966170906	27.945271127473468	27.82447778419641	22.159918122159212
75-79	22.09990409197264	27.86702156970916	27.817652154153734	22.215422184164463
80-84	22.060253026303815	27.93133257671868	27.831417339849384	22.17699705712812
85-89	22.049212148680354	27.927984154433595	27.792801511247987	22.230002185638067
90-94	21.519544828433176	27.600112981914794	28.933947069959494	21.94639511969254
95-99	22.07954654409992	27.901397551199093	27.90300424253293	22.116051662168054
100-104	22.07350681383666	27.9487307102393	27.887027417223088	22.090735058700954
105-109	22.01600468784461	27.979895439557623	27.931869427396904	22.07223044520086
110-114	22.049091538956663	27.802812149475724	27.941247780403067	22.206848531164546
115-119	22.03392604513656	27.891054383205482	27.872955847317133	22.202063724340825
120-124	21.969642230791354	27.942562856433646	27.922290585872144	22.16550432690286
125-129	21.977181756440014	27.9373321997398	27.89159719790365	22.193888845916536
130-134	22.02888803349385	27.950574522356387	27.883785259609713	22.136752184540054
135-139	22.07992525946658	27.942328926861272	27.83437503590011	22.143370777772038
140-144	22.106945939340964	27.962599512371643	27.858891964209825	22.07156258407757
145-149	22.01373148950365	27.998593315829563	27.920866671934025	22.06680852273276
150-154	21.952849629022865	28.10067973736431	27.958023332627924	21.9884473009849
155-159	21.931453642642342	28.088654675042495	27.973917713681267	22.005973968633892
160-164	21.854932350089232	28.225367726125395	28.017720017846298	21.901979905939083
165-169	21.77398245071986	28.243444018922524	28.081642403503356	21.90093112685426
170-174	21.78574365660714	28.318926232943387	28.06988121694294	21.825448893506536
175-178	21.70055433595519	28.384385584276174	28.120433921905608	21.794626157863032
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	8.0
12	26.0
13	48.0
14	114.5
15	275.0
16	540.0
17	1076.0
18	2098.5
19	3889.0
20	6654.0
21	10757.5
22	16462.0
23	23729.5
24	32692.0
25	43409.0
26	55558.5
27	69161.5
28	84303.5
29	100960.0
30	119139.5
31	137440.5
32	157091.0
33	178608.0
34	200370.0
35	222141.5
36	243676.0
37	264769.5
38	284337.5
39	301789.5
40	315479.5
41	326908.0
42	333751.0
43	333980.5
44	330739.0
45	323433.0
46	312581.5
47	298525.5
48	281717.0
49	263103.0
50	243671.5
51	223879.5
52	204749.5
53	187008.5
54	170477.0
55	155814.5
56	142609.5
57	131282.5
58	121486.0
59	111719.0
60	102641.5
61	93985.0
62	85066.5
63	76399.5
64	67695.5
65	58695.5
66	49633.0
67	41188.0
68	33509.0
69	26366.5
70	20158.0
71	14924.5
72	10690.0
73	7531.5
74	5106.5
75	3350.0
76	2154.0
77	1289.0
78	768.5
79	459.0
80	246.0
81	138.0
82	66.5
83	32.0
84	20.5
85	7.0
86	1.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	972.0
92-93	2027.0
94-95	3880.0
96-97	4740.0
98-99	4848.0
100-101	5716.0
102-103	6543.0
104-105	7678.0
106-107	8868.0
108-109	9650.0
110-111	10429.0
112-113	11592.0
114-115	12576.0
116-117	12558.0
118-119	12498.0
120-121	14286.0
122-123	13647.0
124-125	15871.0
126-127	15445.0
128-129	15508.0
130-131	16236.0
132-133	17971.0
134-135	83284.0
136-137	77031.0
138-139	76143.0
140-141	75186.0
142-143	70647.0
144-145	77009.0
146-147	77728.0
148-149	86791.0
150-151	79969.0
152-153	71491.0
154-155	74563.0
156-157	69843.0
158-159	72209.0
160-161	76864.0
162-163	72699.0
164-165	75489.0
166-167	77791.0
168-169	95285.0
170-171	81766.0
172-173	88506.0
174-175	88759.0
176-177	245475.0
178-179	5957378.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.28460522528714
#Duplication Level	Percentage of total
1	91.00657357660154
2	7.837509624807602
3	0.9126826015945231
4	0.1608645079364439
5	0.055319874618101735
6	0.011338582330750394
7	0.0031114321819397973
8	0.007304735645405497
9	0.00314984704447632
>10	0.00214521723921163
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.71496555298191E-5	1.23832185099397E-5	0.0	1.23832185099397E-5	0.0	0.0
2	9.411246067554172E-4	1.23832185099397E-5	0.0	1.23832185099397E-5	0.0	0.0
3	9.411246067554172E-4	2.47664370198794E-5	0.0	2.47664370198794E-5	0.0	0.0
4	9.411246067554172E-4	3.71496555298191E-5	0.0	2.47664370198794E-5	0.0	0.0
5	9.411246067554172E-4	3.71496555298191E-5	0.0	2.47664370198794E-5	1.23832185099397E-5	0.0
6	9.411246067554172E-4	3.71496555298191E-5	1.23832185099397E-5	2.47664370198794E-5	1.23832185099397E-5	0.0
7	9.411246067554172E-4	3.71496555298191E-5	2.47664370198794E-5	2.47664370198794E-5	1.23832185099397E-5	0.0
8	9.535078252653568E-4	3.71496555298191E-5	2.47664370198794E-5	3.71496555298191E-5	1.23832185099397E-5	0.0
9	9.535078252653568E-4	3.71496555298191E-5	2.47664370198794E-5	6.19160925496985E-5	1.23832185099397E-5	0.0
10-14	9.609377563713206E-4	4.457958663578293E-5	2.47664370198794E-5	7.182266735765027E-5	1.23832185099397E-5	0.0
15-19	9.832275496892123E-4	6.686937995367438E-5	3.467301182783116E-5	1.4116869101331257E-4	1.981314961590352E-5	0.0
20-24	9.90657480795176E-4	1.0897232288746937E-4	5.2009517741746735E-5	1.733650591391558E-4	2.47664370198794E-5	0.0
25-29	0.0010129472741130675	1.362154036093367E-4	9.411246067554172E-5	1.8574827764909548E-4	3.219636812584322E-5	0.0
30-34	0.0010748633666627658	1.4612197841728847E-4	1.114489665894573E-4	2.278512205828905E-4	6.934602365566233E-5	0.0
35-39	0.0013051912309476443	1.807949902451196E-4	1.3869204731132464E-4	2.7986073832463715E-4	1.1887889769542111E-4	0.0
40-44	0.001656874636629932	1.8822492135108345E-4	1.857482776490955E-4	3.3682354347035984E-4	1.5355190952325227E-4	2.2289793317891464E-5
45-49	0.0019912215363983034	2.3775779539084223E-4	2.1051471466897488E-4	4.0616956712602215E-4	1.9565485245704727E-4	6.686937995367438E-5
50-54	0.002258699056213001	2.550943013047578E-4	2.154680020729508E-4	4.4827251005981713E-4	2.674775198146975E-4	7.42993110596382E-5
55-59	0.0024543539086700483	2.89767313132589E-4	2.2537457688090253E-4	4.6065572856975685E-4	2.9472060053656486E-4	8.668252956957791E-5
60-64	0.002615335749299265	3.095804627484925E-4	2.451877264968061E-4	5.126652463115036E-4	3.095804627484925E-4	9.90657480795176E-5
65-69	0.002761457727716553	3.095804627484925E-4	2.724308072186734E-4	5.671514077552382E-4	3.9130970491409455E-4	9.90657480795176E-5
70-74	0.004321743259968955	3.3682354347035984E-4	2.89767313132589E-4	5.943944884771056E-4	4.235060730399377E-4	9.90657480795176E-5
75-79	0.032540621600419546	3.838797738081307E-4	3.095804627484925E-4	6.315441440069247E-4	4.631323722717448E-4	1.114489665894573E-4
80-84	0.04465883923424653	4.036929234240342E-4	3.1205710645048045E-4	6.662171558347559E-4	6.241142129009609E-4	1.114489665894573E-4
85-89	0.04480991450006779	4.2102942933794975E-4	3.343468997683719E-4	6.934602365566232E-4	0.0010476202859408985	1.114489665894573E-4
90-94	0.04489659702963737	4.507491537618051E-4	3.467301182783116E-4	7.256566046824665E-4	0.001077340010364754	1.114489665894573E-4
95-99	0.0449312700414652	4.8294552188764826E-4	3.789264864041548E-4	7.4546975429837E-4	0.001077340010364754	1.114489665894573E-4
100-104	0.04498327955920696	4.854221655896362E-4	3.962629923180704E-4	8.197690653580081E-4	0.0010847699414707178	1.114489665894573E-4
105-109	0.04503776572065069	5.027586715035518E-4	4.012162797220463E-4	9.188348134375258E-4	0.0010971531599806574	1.114489665894573E-4
110-114	0.045134354825028214	5.32478395927407E-4	4.235060730399377E-4	0.0010055173430071037	0.0011070597347886092	1.114489665894573E-4
115-119	0.04520617749238587	5.597214766492744E-4	4.457958663578292E-4	0.0010550502170468623	0.0011243962407025247	1.114489665894573E-4
120-124	0.04538697248263099	5.968711321790935E-4	4.97805384099576E-4	0.0011764057584442715	0.0011417327466164405	1.114489665894573E-4
125-129	0.04579066540605502	6.067777069870453E-4	5.176185337154794E-4	0.0012358452072919821	0.0011813590458482474	1.114489665894573E-4
130-134	0.04618940504207508	6.265908566029488E-4	5.349550396293951E-4	0.0012581350006098735	0.001196218908060175	1.114489665894573E-4
135-139	0.04664510748324087	6.439273625168644E-4	5.448616144373468E-4	0.001315097805755596	0.0012135554139740905	1.114489665894573E-4
140-144	0.046964594520797306	6.488806499208402E-4	5.448616144373468E-4	0.0013423408864774635	0.0012358452072919821	1.114489665894573E-4
145-149	0.047269221696141825	6.612638684307799E-4	5.572448329472865E-4	0.0013918737605172222	0.0012556583569078856	1.114489665894573E-4
150-154	0.04753422257225453	6.909835928546352E-4	6.043010632850573E-4	0.0014513132093649328	0.0012655649317158374	1.114489665894573E-4
155-159	0.04792305563346664	7.033668113645749E-4	6.067777069870453E-4	0.0014785562900868	0.0012878547250337288	1.1392561029144525E-4
160-164	0.048361421568718505	7.107967424705388E-4	6.067777069870453E-4	0.0014983694397027038	0.001327481024265536	1.23832185099397E-4
165-167	0.048798136408169046	7.182266735765026E-4	6.067777069870453E-4	0.001535519095232523	0.0013538985570867405	1.3208766410602345E-4
>>END_MODULE
