##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293513
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9189906
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.44074553102067	39.0	39.0	39.0	37.0	39.0
2	40.27563611640859	41.0	40.0	41.0	39.0	41.0
3	40.32102787558436	41.0	40.0	41.0	39.0	41.0
4	40.33917844208635	41.0	40.0	41.0	39.0	41.0
5	40.346288090433134	41.0	40.0	41.0	39.0	41.0
6	40.33622346082756	41.0	40.0	41.0	39.0	41.0
7	40.36116375945521	41.0	40.0	41.0	39.0	41.0
8	40.329423935348196	41.0	40.0	41.0	39.0	41.0
9	40.33832859661459	41.0	40.0	41.0	39.0	41.0
10-14	40.292935161687176	41.0	40.0	41.0	39.0	41.0
15-19	40.20884394247341	41.0	40.0	41.0	38.2	41.0
20-24	40.098635981695566	41.0	40.0	41.0	38.0	41.0
25-29	39.94314496796812	41.0	40.0	41.0	38.0	41.0
30-34	39.70117803163601	41.0	39.6	41.0	36.8	41.0
35-39	39.33806720112263	40.0	38.6	41.0	35.4	41.0
40-44	38.90416474336081	40.0	37.6	41.0	35.0	41.0
45-49	38.39947100655871	39.2	36.2	41.0	35.0	41.0
50-54	37.8107783692238	38.0	35.0	40.8	35.0	41.0
55-59	37.05103416727004	36.6	35.0	39.4	35.0	41.0
60-64	36.17908557497759	35.4	34.8	38.0	34.2	40.2
65-69	35.71161023845075	35.0	35.0	36.6	33.8	39.0
70-74	35.432989042542985	35.0	35.0	36.0	34.0	37.6
75-79	35.217355150313836	35.0	35.0	35.2	34.0	36.8
80-84	35.11994686343908	35.0	35.0	35.0	34.0	36.0
85-89	35.01136412059057	35.0	34.8	35.0	33.8	36.0
90-94	39.75061757729793	40.6	39.8	40.6	37.8	40.6
95-99	40.08280366837853	41.0	40.0	41.0	38.0	41.0
100-104	39.970450047120906	41.0	40.0	41.0	38.0	41.0
105-109	39.85429487318465	41.0	40.0	41.0	37.6	41.0
110-114	39.689495155521215	41.0	39.8	41.0	36.8	41.0
115-119	39.47051072284794	40.4	39.0	41.0	35.8	41.0
120-124	39.1787336658539	40.0	38.2	41.0	35.0	41.0
125-129	38.343459881043735	39.4	37.2	40.4	34.4	40.6
130-134	38.402204675796504	39.8	36.4	40.8	34.8	41.0
135-139	38.17461048529549	39.0	35.6	41.0	35.0	41.0
140-144	37.53096572317116	37.6	35.0	40.6	35.0	41.0
145-149	36.77040482954099	36.2	35.0	39.0	34.4	41.0
150-154	36.06528496320173	35.0	35.0	37.2	34.0	39.6
155-159	35.49538748147725	35.0	35.0	36.2	34.0	38.2
160-164	35.09738436491481	35.0	35.0	35.4	34.0	36.6
165-169	34.869006654096616	35.0	35.0	35.0	34.0	36.0
170-174	34.752072565718805	35.0	35.0	35.0	34.0	36.0
175-178	34.58473995903644	35.0	34.75	35.0	33.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	26.0
30	303.0
31	2052.0
32	10313.0
33	39493.0
34	118633.0
35	326020.0
36	1008334.0
37	2872734.0
38	3737883.0
39	1058748.0
40	15365.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.32619201980956	27.61610401673314	28.540139583582246	22.51756437987505
2	22.050192896423532	28.153400045658795	29.02038388640754	20.776023171510133
3	20.579688192675746	27.028535438773805	29.85807471806567	22.533701650484783
4	21.301306019887473	26.435417293713343	30.15755547445208	22.105721211947106
5	22.161206001454204	27.033290656074175	28.448299688810746	22.357203653660875
6	22.602733912621087	26.996402357107897	27.848075921560024	22.552787808710992
7	22.40500610125936	27.567964242506942	27.743461140951826	22.283568515281875
8	22.394255175188952	27.628857139561603	27.70378717693086	22.27310050831858
9	22.78313836942402	27.53961792427474	27.240050115855375	22.437193590445865
10-14	22.51447185640419	27.69643998534914	27.426404579111036	22.36268357913563
15-19	22.482815384618732	27.662548452617468	27.539152195898414	22.315483966865386
20-24	22.386087518196597	27.586179880403567	27.673351609907655	22.35438099149219
25-29	22.314892013041266	27.69980019382135	27.596119046266633	22.38918874687075
30-34	22.37086864653458	27.76622089496889	27.52322167386696	22.339688784629573
35-39	22.28634982773491	27.776810774778326	27.56929613861121	22.367543258875553
40-44	22.275150583694764	27.837925654517033	27.56983586121556	22.31708790057265
45-49	22.465267871075067	27.724825476996173	27.449712760935746	22.36019389099301
50-54	22.399162733547005	27.764236108617435	27.518477338070706	22.31812381976486
55-59	22.35295769075331	27.77686082969728	27.529820217965234	22.340361261584178
60-64	22.39374374449532	27.780419081544466	27.49669691942442	22.329140254535794
65-69	22.37448783480484	27.73153501243647	27.525360977576916	22.36861617518177
70-74	22.300622008538497	27.81252822390131	27.539147843296764	22.347701924263426
75-79	22.38505377530521	27.78051048617908	27.46123627379867	22.37319946471705
80-84	22.352065407415484	27.799524826478095	27.46061385176301	22.38779591434341
85-89	22.321114057096995	27.849468754087365	27.459956608914172	22.36946057990147
90-94	21.350870592162163	27.30876965308186	29.233841940798605	22.10651781395737
95-99	22.267388631473402	27.58442396718946	27.770283954161094	22.377903447176042
100-104	22.33710717261915	27.697769169348568	27.589001792195926	22.376121865836353
105-109	22.171673341073806	27.72862835228111	27.785909553256378	22.31378875338871
110-114	22.228002710512474	27.591497111968756	27.792646451238184	22.387853726280586
115-119	22.189293020269044	27.637742770065397	27.732118319534386	22.440845890131172
120-124	22.10871169137442	27.727291106173325	27.76288537544554	22.40111182700672
125-129	22.14517846493655	27.696807147826245	27.735431812678506	22.4225825745587
130-134	22.241624111467146	27.676454720463795	27.69478839353392	22.387132774535136
135-139	22.267037563666698	27.686061229857785	27.673550513376682	22.373350693098836
140-144	22.308022297211153	27.72358981754734	27.666700153186508	22.301687732054994
145-149	22.232984020460943	27.728017783841697	27.699773717581472	22.33922447811589
150-154	22.136075222960688	27.827614263286428	27.763993573520118	22.272316940232766
155-159	22.209786669088665	27.79790385627907	27.714383411184834	22.27792606344743
160-164	22.141479630691723	27.886990493709312	27.72812287195333	22.24340700364563
165-169	22.05584793271753	27.924915526022513	27.788563705090937	22.230672836169024
170-174	22.11937319172299	27.967732753553324	27.72768013936743	22.185213915356258
175-178	22.046455354611634	28.037754545308662	27.74484356863563	22.17094653144407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	6.0
12	14.0
13	34.0
14	75.5
15	166.0
16	319.0
17	628.0
18	1257.0
19	2310.5
20	4190.5
21	7093.5
22	11328.5
23	17421.0
24	25272.5
25	34972.5
26	46819.0
27	60791.5
28	76373.0
29	93304.0
30	111989.0
31	131482.5
32	151810.5
33	173728.0
34	196733.0
35	220511.5
36	245763.5
37	271437.0
38	296706.5
39	321861.5
40	345742.5
41	366927.0
42	384984.5
43	398900.5
44	406738.0
45	408623.5
46	405783.0
47	397048.0
48	382677.0
49	363669.0
50	341118.0
51	315429.5
52	288222.5
53	261010.5
54	233209.0
55	207180.5
56	182692.5
57	159452.5
58	138246.0
59	119536.0
60	103105.0
61	88486.0
62	75453.5
63	63562.0
64	53294.5
65	44357.5
66	36163.5
67	29087.0
68	23097.0
69	17890.0
70	13477.5
71	9924.5
72	7137.0
73	4968.5
74	3353.5
75	2163.5
76	1293.0
77	797.0
78	491.5
79	272.0
80	152.5
81	78.5
82	35.0
83	20.0
84	13.0
85	2.5
86	1.5
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	592.0
92-93	1135.0
94-95	1787.0
96-97	2262.0
98-99	2997.0
100-101	3407.0
102-103	4134.0
104-105	4083.0
106-107	4747.0
108-109	5590.0
110-111	5985.0
112-113	5450.0
114-115	6004.0
116-117	6145.0
118-119	7091.0
120-121	7138.0
122-123	7743.0
124-125	7598.0
126-127	8035.0
128-129	8429.0
130-131	8743.0
132-133	9896.0
134-135	90597.0
136-137	76364.0
138-139	87054.0
140-141	87174.0
142-143	86629.0
144-145	60798.0
146-147	66029.0
148-149	70072.0
150-151	68875.0
152-153	66204.0
154-155	68906.0
156-157	64911.0
158-159	64066.0
160-161	66756.0
162-163	82010.0
164-165	69509.0
166-167	71538.0
168-169	70628.0
170-171	82103.0
172-173	75722.0
174-175	81504.0
176-177	141340.0
178-179	7372126.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.4453034849296
#Duplication Level	Percentage of total
1	88.14490060408299
2	9.094935273531394
3	1.5956529409610603
4	0.5178659622664846
5	0.22869501567373698
6	0.10778420419594746
7	0.06398973313415506
8	0.03735678984114318
9	0.025039311293555324
>10	0.17159923865353252
>50	0.010316561264199322
>100	0.0018643651018413062
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.0881504119846275E-5	1.0881504119846275E-5	0.0	1.0881504119846275E-5	0.0
2	2.176300823969255E-5	1.0881504119846275E-5	1.0881504119846275E-5	0.0	1.0881504119846275E-5	0.0
3	3.264451235953882E-5	1.0881504119846275E-5	1.0881504119846275E-5	0.0	1.0881504119846275E-5	0.0
4	3.264451235953882E-5	1.0881504119846275E-5	1.0881504119846275E-5	0.0	2.176300823969255E-5	0.0
5	4.35260164793851E-5	2.176300823969255E-5	1.0881504119846275E-5	0.0	3.264451235953882E-5	0.0
6	4.35260164793851E-5	4.35260164793851E-5	2.176300823969255E-5	1.0881504119846275E-5	3.264451235953882E-5	0.0
7	4.35260164793851E-5	7.617052883892393E-5	3.264451235953882E-5	2.176300823969255E-5	3.264451235953882E-5	0.0
8	5.440752059923138E-5	7.617052883892393E-5	3.264451235953882E-5	2.176300823969255E-5	4.35260164793851E-5	0.0
9	5.440752059923138E-5	8.70520329587702E-5	3.264451235953882E-5	2.176300823969255E-5	4.35260164793851E-5	0.0
10-14	6.964162636701615E-5	1.0446243955052425E-4	3.482081318350808E-5	3.0468211535569572E-5	4.35260164793851E-5	0.0
15-19	7.617052883892393E-5	1.5234105767784786E-4	5.2231219775262125E-5	4.7878618127323616E-5	4.35260164793851E-5	0.0
20-24	1.1969654531830904E-4	1.9151447250929444E-4	6.74653255430469E-5	5.876012224716989E-5	5.2231219775262125E-5	0.0
25-29	1.305780494381553E-4	1.9586707415723297E-4	1.0228613872655499E-4	8.70520329587702E-5	6.31127238951084E-5	0.0
30-34	3.286214244193575E-4	2.13277480748987E-4	1.3710695191006305E-4	9.793353707861648E-5	9.575723625464722E-5	8.70520329587702E-6
35-39	9.466908584266258E-4	2.3286418816471027E-4	1.6322256179769412E-4	1.1534394367037051E-4	1.4581215520594008E-4	1.741040659175404E-5
40-44	0.001627873016329003	2.611560988763106E-4	1.915144725092944E-4	1.2187284614227829E-4	1.8716187086135593E-4	2.176300823969255E-5
45-49	0.0020740146852427	3.046821153556957E-4	2.2415898486883326E-4	1.392832527340323E-4	2.3504048898867957E-4	2.3939309063661805E-5
50-54	0.002489688142620828	3.199162211234805E-4	2.568034972283721E-4	1.5669365932578636E-4	3.0250581453172647E-4	4.7878618127323616E-5
55-59	0.002763902046440954	3.395029285392038E-4	2.807428062920339E-4	1.697514642696019E-4	3.438555301871423E-4	6.528902471907765E-5
60-64	0.0030228818444932954	3.7432374172271187E-4	3.046821153556957E-4	1.936907733332637E-4	3.7867634337065037E-4	7.617052883892393E-5
65-69	0.003403734488687915	3.9826305078637363E-4	3.307977252433268E-4	2.089248791010485E-4	4.4178906726575877E-4	1.0881504119846275E-4
70-74	0.0063613273084621325	4.2873126232194326E-4	3.5473703430698855E-4	2.176300823969255E-4	5.375463035204059E-4	1.2404914696624754E-4
75-79	0.0617111861644722	4.6137577468148203E-4	3.7867634337065037E-4	2.2415898486883326E-4	6.093642307113914E-4	1.3275435026212454E-4
80-84	0.08586594900970695	4.7878618127323604E-4	4.0261565243431214E-4	2.611560988763106E-4	7.812919958049625E-4	1.4798845602990936E-4
85-89	0.08602917157150465	4.8531508374514385E-4	4.1349715655415845E-4	3.003295137077572E-4	0.0010685637045689041	1.5234105767784786E-4
90-94	0.08609010799457578	5.288411002245289E-4	4.1349715655415845E-4	3.1773992029951123E-4	0.0010881504119846276	1.5234105767784786E-4
95-99	0.08617716002753455	5.658382142320063E-4	4.2220235985003545E-4	3.351503268912653E-4	0.0010990319161044738	1.5234105767784786E-4
100-104	0.08628379876790905	6.050116290634529E-4	4.3743646561782026E-4	3.438555301871423E-4	0.00110991342022432	1.5234105767784786E-4
105-109	0.08639261380910752	6.137168323593299E-4	4.483179697376665E-4	3.7867634337065037E-4	0.0011207949243441664	1.5234105767784786E-4
110-114	0.08646225543547453	6.768295562544383E-4	4.6572837632942053E-4	4.2655496149797395E-4	0.0011403816317598896	1.5886996014975561E-4
115-119	0.08657107047667299	7.42118580973516E-4	4.940202870410209E-4	4.4614166891369727E-4	0.0011882602498872131	1.6322256179769412E-4
120-124	0.08707597226783385	7.725867925090855E-4	5.070780919848364E-4	4.504942705616358E-4	0.0012666070795501062	1.6322256179769412E-4
125-129	0.08790949548341408	8.008787032206859E-4	5.223121977526212E-4	4.570231730335435E-4	0.001288370087789799	1.6539886262166337E-4
130-134	0.08874301869899433	8.552862238199172E-4	5.484278076402524E-4	4.6137577468148203E-4	0.0013449539092129995	1.741040659175404E-4
135-139	0.0895787182153985	9.118700452431178E-4	5.571330109361293E-4	4.896676853930824E-4	0.0013797747223965077	1.741040659175404E-4
140-144	0.09032083679637201	9.488671592505951E-4	5.832486208237604E-4	5.049017911608672E-4	0.0014254770396998622	1.784566675654789E-4
145-149	0.09089973281554783	9.923931757299803E-4	6.006590274155144E-4	5.114306936327749E-4	0.0014798845602990935	1.8498557003738668E-4
150-154	0.0913480507852855	0.0010446243955052423	6.22422035655207E-4	5.266647994005598E-4	0.00151905797513054	1.8498557003738668E-4
155-159	0.09183336586903065	0.0010663874037449349	6.39832442246961E-4	5.440752059923138E-4	0.001536468381722294	1.9151447250929444E-4
160-164	0.09244273009974205	0.0010990319161044738	6.637717513106227E-4	5.440752059923138E-4	0.0015756417965537405	2.0021967580517147E-4
165-167	0.09311303075352458	0.00110991342022432	6.673989193505715E-4	5.440752059923138E-4	0.0016068354416973	2.1037574631702798E-4
>>END_MODULE
