##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293514
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9320739
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.52372156327948	39.0	39.0	39.0	38.0	39.0
2	40.320861253598025	41.0	40.0	41.0	39.0	41.0
3	40.341256524831344	41.0	40.0	41.0	39.0	41.0
4	40.347890655451245	41.0	40.0	41.0	39.0	41.0
5	40.34058780103166	41.0	40.0	41.0	39.0	41.0
6	40.340034411434544	41.0	40.0	41.0	39.0	41.0
7	40.366932707803535	41.0	40.0	41.0	39.0	41.0
8	40.33196831281297	41.0	40.0	41.0	39.0	41.0
9	40.33960225685968	41.0	40.0	41.0	39.0	41.0
10-14	40.285808603802764	41.0	40.0	41.0	39.0	41.0
15-19	40.20533191627832	41.0	40.0	41.0	38.2	41.0
20-24	40.10300327044884	41.0	40.0	41.0	38.0	41.0
25-29	39.940627561827455	41.0	40.0	41.0	38.0	41.0
30-34	39.702088471740275	41.0	39.6	41.0	36.8	41.0
35-39	39.33766841878096	40.0	38.6	41.0	35.4	41.0
40-44	38.905169064384275	40.0	37.6	41.0	35.0	41.0
45-49	38.40385699030946	39.4	36.2	41.0	35.0	41.0
50-54	37.81800548218333	38.0	35.0	40.8	35.0	41.0
55-59	37.03486264340199	36.6	35.0	39.4	35.0	41.0
60-64	36.155795972830056	35.4	34.8	38.0	34.0	40.2
65-69	35.67499452564867	35.0	35.0	36.6	33.8	39.0
70-74	35.38180504786154	35.0	35.0	36.0	34.0	37.2
75-79	35.157214722995676	35.0	35.0	35.0	34.0	36.6
80-84	35.05663467242243	35.0	35.0	35.0	34.0	36.0
85-89	34.94455731460778	35.0	34.8	35.0	33.8	36.0
90-94	39.78012846435049	40.6	39.8	40.6	37.8	40.6
95-99	40.10142015940951	41.0	40.0	41.0	38.0	41.0
100-104	39.99076462233811	41.0	40.0	41.0	38.0	41.0
105-109	39.879567378121735	41.0	40.0	41.0	37.8	41.0
110-114	39.71556862926961	41.0	39.8	41.0	37.0	41.0
115-119	39.50201522053336	40.4	39.0	41.0	36.2	41.0
120-124	39.21364464492774	40.0	38.4	41.0	35.0	41.0
125-129	38.37300562057037	39.4	37.2	40.4	34.4	40.6
130-134	38.45177736812678	39.8	36.8	40.8	34.8	41.0
135-139	38.22813292214945	39.0	35.6	41.0	35.0	41.0
140-144	37.56980886100905	37.6	35.0	40.6	35.0	41.0
145-149	36.795092043301736	36.2	35.0	39.2	34.2	41.0
150-154	36.07567259361774	35.2	35.0	37.2	34.0	39.8
155-159	35.49725713316772	35.0	35.0	36.2	34.0	38.2
160-164	35.09577046949351	35.0	35.0	35.6	34.0	36.8
165-169	34.85902470671271	35.0	35.0	35.0	34.0	36.0
170-174	34.734415829111846	35.0	35.0	35.0	34.0	36.0
175-178	34.55826779955856	35.0	34.75	35.0	33.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	28.0
30	247.0
31	1947.0
32	10120.0
33	39116.0
34	119671.0
35	333838.0
36	1029093.0
37	2929633.0
38	3790726.0
39	1053262.0
40	13058.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.291112217604205	27.656744813903707	28.598987698293023	22.45315527019907
2	22.031686543309494	28.168978876031183	29.05840406002142	20.740930520637903
3	20.54113949548421	27.067735723530078	29.871891059281886	22.519233721703827
4	21.26229476010432	26.461903932724645	30.211595883116132	22.064205424054894
5	22.147170948569634	27.038220896433213	28.487816255771136	22.326791899226016
6	22.559241279044507	27.06032214827601	27.876405508189855	22.50403106448963
7	22.376723562369893	27.57571046673445	27.78942742630171	22.25813854459394
8	22.357637092938663	27.679361046371966	27.750514202790143	22.21248765789923
9	22.768280497930476	27.563404575538485	27.233173249460158	22.435141677070884
10-14	22.498683848995235	27.727301451097386	27.447021099936393	22.32699359997099
15-19	22.461150344409386	27.68990312892572	27.566466564507387	22.282479962157506
20-24	22.35662000620337	27.61750758174861	27.70912907227635	22.316743339771662
25-29	22.302519145745848	27.73171955571334	27.617174990094668	22.348586308446144
30-34	22.347457642575336	27.782878589347902	27.56634211085623	22.303321657220526
35-39	22.25402299109545	27.78652636877827	27.61310664315351	22.346343996972774
40-44	22.237979198859662	27.862788562151565	27.604444239882696	22.29478799910608
45-49	22.435515038024345	27.764060338992437	27.471351788736925	22.329072834246297
50-54	22.374951170717257	27.797012661764263	27.53159164740049	22.296444520117987
55-59	22.32926380622824	27.798467482031196	27.551746701629558	22.320522010111002
60-64	22.378804942397807	27.787449042398894	27.520489523416547	22.313256491786756
65-69	22.347513432143096	27.765616009631856	27.53967255171505	22.34719800651
70-74	22.275538452476784	27.84017662118851	27.558364202666763	22.32592072366794
75-79	22.361632484291214	27.791656863259444	27.49398304147343	22.35272761097591
80-84	22.318629456312422	27.821939869789297	27.4944658358098	22.364964838088483
85-89	22.2951120077496	27.88508293172891	27.479803908252336	22.34000115226915
90-94	21.316837385318177	27.344235744999395	29.269092528583517	22.06983434109891
95-99	22.237174332035615	27.62398900984866	27.796580617591925	22.3422560405238
100-104	22.290283334319717	27.72256400765749	27.606889595242674	22.38026306278012
105-109	22.13793833946798	27.772509378078787	27.809846661256877	22.27970562119636
110-114	22.18413480422788	27.615167811517445	27.81728071809932	22.383416666155355
115-119	22.158736471967757	27.685011513544623	27.746547305656357	22.409704708831267
120-124	22.067274045742888	27.773053500740396	27.795679208878955	22.363993244637765
125-129	22.103066921289678	27.74381022664294	27.7514078882424	22.401714963824983
130-134	22.205560940462927	27.704492429764915	27.712404592611456	22.377542037160705
135-139	22.23580137436115	27.71427061580689	27.704072713044564	22.345855296787402
140-144	22.26726282969385	27.74751205201815	27.707617074182263	22.277608044105747
145-149	22.208236488870213	27.7445841605156	27.727881025375183	22.319298325239
150-154	22.112819271656612	27.867569398771806	27.778500600288115	22.241110729283474
155-159	22.17902745807796	27.826251554589426	27.749609512674418	22.245111474658195
160-164	22.09349329204495	27.922682226687414	27.761860659908088	22.22196382135955
165-169	22.007811633640927	27.980132372863075	27.795286940156345	22.21676905333965
170-174	22.064207025385734	28.00594756313302	27.77704473937587	22.15280067210537
175-178	21.98467256616264	28.095287099198487	27.763740962889464	22.156299371749405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.5
11	5.5
12	16.5
13	34.0
14	83.0
15	176.5
16	331.5
17	658.0
18	1331.5
19	2473.0
20	4450.5
21	7439.5
22	11809.0
23	17992.0
24	26101.5
25	36173.5
26	47950.0
27	62256.5
28	78768.5
29	95989.0
30	114627.5
31	134781.5
32	155693.0
33	178198.0
34	201276.5
35	224647.5
36	249835.5
37	276308.5
38	302211.5
39	327276.0
40	351799.0
41	373612.5
42	391230.5
43	404685.0
44	411716.0
45	414049.5
46	411066.0
47	401072.5
48	387372.0
49	368687.5
50	344660.0
51	318222.0
52	291214.5
53	263352.0
54	235649.0
55	209301.0
56	183866.5
57	160033.0
58	139295.0
59	120492.5
60	103656.5
61	89246.0
62	75652.5
63	63818.5
64	53681.5
65	44353.0
66	36174.5
67	29269.5
68	23203.5
69	17907.0
70	13471.0
71	9781.0
72	7014.5
73	4923.0
74	3309.5
75	2120.5
76	1331.5
77	831.5
78	490.0
79	263.0
80	137.5
81	73.5
82	34.5
83	15.5
84	7.0
85	3.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	523.0
92-93	1036.0
94-95	1528.0
96-97	2067.0
98-99	2414.0
100-101	2965.0
102-103	3351.0
104-105	3580.0
106-107	4087.0
108-109	4429.0
110-111	4804.0
112-113	5156.0
114-115	5344.0
116-117	5686.0
118-119	6188.0
120-121	6372.0
122-123	6614.0
124-125	7213.0
126-127	9661.0
128-129	8023.0
130-131	8267.0
132-133	9042.0
134-135	69404.0
136-137	66778.0
138-139	70734.0
140-141	69426.0
142-143	65146.0
144-145	62272.0
146-147	61612.0
148-149	61733.0
150-151	65888.0
152-153	61788.0
154-155	61519.0
156-157	74331.0
158-159	67933.0
160-161	62993.0
162-163	64667.0
164-165	69313.0
166-167	70364.0
168-169	70858.0
170-171	77395.0
172-173	77133.0
174-175	77389.0
176-177	142975.0
178-179	7640738.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.28517717606763
#Duplication Level	Percentage of total
1	87.83997420345233
2	9.343277679811928
3	1.680220168834858
4	0.4842433426647834
5	0.22147932626019265
6	0.11595136234485152
7	0.06808650982900737
8	0.04252227493329434
9	0.020853257246377297
>10	0.1654639728059704
>50	0.017927901816313308
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	2.145752606097006E-5	0.0
2	1.072876303048503E-5	2.145752606097006E-5	2.145752606097006E-5	0.0	2.145752606097006E-5	0.0
3	1.072876303048503E-5	3.2186289091455086E-5	2.145752606097006E-5	0.0	2.145752606097006E-5	0.0
4	1.072876303048503E-5	4.291505212194012E-5	3.2186289091455086E-5	1.072876303048503E-5	3.2186289091455086E-5	0.0
5	2.145752606097006E-5	4.291505212194012E-5	4.291505212194012E-5	1.072876303048503E-5	3.2186289091455086E-5	0.0
6	2.145752606097006E-5	4.291505212194012E-5	4.291505212194012E-5	1.072876303048503E-5	3.2186289091455086E-5	0.0
7	3.2186289091455086E-5	4.291505212194012E-5	4.291505212194012E-5	2.145752606097006E-5	3.2186289091455086E-5	0.0
8	3.2186289091455086E-5	4.291505212194012E-5	4.291505212194012E-5	3.2186289091455086E-5	5.364381515242515E-5	0.0
9	3.2186289091455086E-5	4.291505212194012E-5	5.364381515242515E-5	4.291505212194012E-5	5.364381515242515E-5	0.0
10-14	4.291505212194012E-5	4.506080472803713E-5	7.939284642558922E-5	5.1498062546328137E-5	7.08098360012012E-5	0.0
15-19	5.793532036461916E-5	6.008107297071617E-5	9.655886727436526E-5	6.866408339510418E-5	7.724709381949222E-5	0.0
20-24	8.583010424388023E-5	7.939284642558922E-5	9.655886727436526E-5	8.797585684997723E-5	8.583010424388023E-5	0.0
25-29	1.0299612509265627E-4	1.1587064072923832E-4	9.655886727436526E-5	1.2659940375972333E-4	9.226736206217124E-5	0.0
30-34	2.8753084921699873E-4	1.3303666157801435E-4	1.094333829109473E-4	1.5449418763898443E-4	1.0299612509265627E-4	0.0
35-39	8.218232481351532E-4	1.5878569285117844E-4	1.3518241418411137E-4	1.8238897151824548E-4	1.6093144545727546E-4	4.291505212194011E-6
40-44	0.0012209332328691964	1.8024321891214852E-4	1.416196720024024E-4	2.0599225018531255E-4	2.4676154970115565E-4	3.433204169755209E-5
45-49	0.0016286262280276274	1.8882622933653648E-4	1.5449418763898443E-4	2.145752606097006E-4	3.6477794303649096E-4	5.793532036461916E-5
50-54	0.001890408045971462	2.059922501853126E-4	1.6093144545727543E-4	2.231582710340886E-4	4.291505212194012E-4	6.437257818291017E-5
55-59	0.0020835257805201924	2.4676154970115565E-4	1.780974663060515E-4	2.725105809743197E-4	4.7206557334134123E-4	7.08098360012012E-5
60-64	0.0022508944837957593	2.875308492169988E-4	1.8668047673043952E-4	3.111341278840659E-4	5.106891202510873E-4	1.072876303048503E-4
65-69	0.0023989514136164526	2.939681070352898E-4	2.295955288523796E-4	3.7765245867307296E-4	5.578956775852215E-4	1.2445365115362634E-4
70-74	0.0054437743616681045	3.4332041697552094E-4	2.574903127316407E-4	3.9481847952184905E-4	6.329970187986167E-4	1.459111772145964E-4
75-79	0.06207233138917419	3.7979821127917E-4	2.617818179438347E-4	3.96964232127946E-4	6.82349328738848E-4	1.6093144545727543E-4
80-84	0.08706820349759821	4.034014899462371E-4	2.896766018230958E-4	4.248590160072071E-4	7.767624434071161E-4	1.6093144545727543E-4
85-89	0.0872141146748128	4.527537998864683E-4	3.0040536485358084E-4	4.827943363718263E-4	9.913377040168168E-4	1.6093144545727543E-4
90-94	0.08724200945869207	4.913773467962142E-4	3.197171383084539E-4	5.085433676449904E-4	0.001021378240502175	1.6093144545727543E-4
95-99	0.08730852778948107	4.978146046145053E-4	3.347374065511329E-4	5.300008937059606E-4	0.0010492730243814358	1.6093144545727543E-4
100-104	0.08739650364633104	5.643329354035125E-4	3.5190342739990896E-4	5.428754093425426E-4	0.0010578560348058238	1.6093144545727543E-4
105-109	0.08745873047190786	6.265597609803256E-4	3.71215200854782E-4	5.557499249791246E-4	0.001062147540018018	1.6093144545727543E-4
110-114	0.08752954030790906	6.780578235266539E-4	3.84089716491364E-4	5.857904614644827E-4	0.0010707305504424058	1.6093144545727543E-4
115-119	0.08760464164912246	7.016611021937208E-4	4.076929951584311E-4	5.900819666766766E-4	0.001072876303048503	1.6093144545727543E-4
120-124	0.08801877190209917	7.145356178303031E-4	4.2700476861330417E-4	5.986649771010647E-4	0.0010857508186850851	1.6522295066946944E-4
125-129	0.08869468397301974	7.402846491034671E-4	4.377335316437892E-4	6.008107297071617E-4	0.001124374365594831	1.7809746630605147E-4
130-134	0.08945857190079028	7.51013412133952E-4	4.4417078946208015E-4	6.244140083742286E-4	0.0012037672120204202	1.9311773454873052E-4
135-139	0.09019027353946935	7.51013412133952E-4	4.6991982073524426E-4	6.415800292230047E-4	0.0012638482849911365	2.017007449731186E-4
140-144	0.09078464701135822	7.789081960132131E-4	4.7206557334134134E-4	6.608918026778778E-4	0.0013046175845069796	2.1242950800360355E-4
145-149	0.09147772510312754	8.046572272863772E-4	4.935230994023113E-4	6.737663183144598E-4	0.0013561156470533078	2.145752606097006E-4
150-154	0.09184464879877015	8.325520111656384E-4	5.128348728571844E-4	6.80203576132751E-4	0.0014054679569935388	2.145752606097006E-4
155-159	0.09229954835126271	8.754670632875784E-4	5.321466463120575E-4	7.038068547998179E-4	0.00147627779299474	2.253040236401856E-4
160-164	0.09272655311987602	8.904873315302573E-4	5.450211619486394E-4	7.18827123042497E-4	0.0015320673607532621	2.253040236401856E-4
165-167	0.09331520458481529	9.298261293087026E-4	5.578956775852215E-4	7.224033773859921E-4	0.0015842806741682893	2.3245653232717564E-4
>>END_MODULE
