##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293515
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6064146
Total Bases	991.3 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.57262292167768	39.0	39.0	39.0	38.0	39.0
2	40.31573184418713	41.0	40.0	41.0	39.0	41.0
3	40.42707233631908	41.0	40.0	41.0	39.0	41.0
4	40.445767301776705	41.0	40.0	41.0	39.0	41.0
5	40.453932837369024	41.0	40.0	41.0	39.0	41.0
6	40.44381500709251	41.0	40.0	41.0	39.0	41.0
7	40.46634348843184	41.0	40.0	41.0	39.0	41.0
8	40.43102870544344	41.0	40.0	41.0	39.0	41.0
9	40.42452754270758	41.0	40.0	41.0	39.0	41.0
10-14	40.391373261791514	41.0	40.0	41.0	39.0	41.0
15-19	40.31469766723954	41.0	40.0	41.0	39.0	41.0
20-24	40.18890006935849	41.0	40.0	41.0	38.0	41.0
25-29	40.00559801825352	41.0	40.0	41.0	38.0	41.0
30-34	39.727712591352514	41.0	39.4	41.0	37.2	41.0
35-39	39.40120960148387	40.2	38.8	41.0	35.8	41.0
40-44	38.80906515113587	40.0	37.4	41.0	35.0	41.0
45-49	38.07847782688609	39.0	35.8	40.8	34.6	41.0
50-54	37.23091699968965	37.4	35.0	40.0	33.8	41.0
55-59	36.356997803153156	36.2	35.0	39.0	33.0	40.6
60-64	35.39806139232136	34.8	33.8	37.2	32.2	39.8
65-69	34.945896322417035	35.0	33.8	36.0	31.4	39.0
70-74	34.826113487373156	35.0	34.0	36.0	31.0	39.6
75-79	34.75134642206834	35.0	33.0	36.0	30.8	40.4
80-84	35.02492466375315	35.0	33.0	38.2	30.0	41.0
85-89	36.05178941931807	35.8	33.8	40.2	30.2	41.0
90-94	39.954330386549614	40.8	39.8	41.0	38.0	41.0
95-99	39.96739527804195	41.0	40.0	41.0	38.0	41.0
100-104	39.7811614272744	41.0	40.0	41.0	37.2	41.0
105-109	39.545252772108974	41.0	39.0	41.0	36.2	41.0
110-114	39.29865112732671	40.2	38.8	41.0	35.2	41.0
115-119	38.978351793732585	40.0	38.0	41.0	35.0	41.0
120-124	38.68425311964348	40.0	37.2	41.0	34.8	41.0
125-129	38.033793931820775	39.4	35.8	40.4	34.0	41.0
130-134	37.72837796437945	38.8	35.0	41.0	34.0	41.0
135-139	37.09945785928585	37.4	35.0	40.0	33.0	41.0
140-144	36.36706602338236	36.0	35.0	39.2	33.0	41.0
145-149	35.636746368512796	35.0	34.2	37.6	32.4	39.8
150-154	34.947857923282385	35.0	34.0	36.4	31.6	38.8
155-159	34.35501066899475	35.0	33.8	35.2	31.0	37.0
160-164	33.868202498172664	34.6	33.0	35.0	31.0	36.0
165-169	33.449998562566776	34.0	33.0	35.0	30.0	35.2
170-174	33.09325782299877	34.0	32.0	35.0	29.4	35.0
175-178	31.77814906293907	32.75	29.75	34.25	27.0	34.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	40.0
31	408.0
32	3158.0
33	16665.0
34	70070.0
35	261505.0
36	966852.0
37	2362764.0
38	2073561.0
39	307084.0
40	2037.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.146192720294003	27.564755202133984	28.697824887461483	22.591227190110526
2	22.04346663157516	28.03710530716114	29.09257132001769	20.826856741246004
3	20.616901374076416	26.895724476290646	29.855712576840993	22.63166157279195
4	21.31901177841035	26.38147234581753	30.1846954212514	22.11482045452072
5	22.1714978498209	26.944898754086726	28.474809148724322	22.408794247368053
6	22.64815194093282	26.92629761882382	27.847630977222515	22.577919463020844
7	22.45160324306176	27.50438396437025	27.776227683172532	22.26778510939545
8	22.380430814165752	27.61701317877241	27.715559618782265	22.28699638827957
9	22.85207183336285	27.43461321676622	27.236547405026197	22.47676754484473
10-14	22.54930867429643	27.647160869807553	27.41788538732412	22.385645068571897
15-19	22.522762479663253	27.601192319578054	27.54625960522718	22.329785595531508
20-24	22.42108946585389	27.516362567787784	27.689956673206744	22.372591293151583
25-29	22.343030659222254	27.65394500726071	27.613250076762665	22.38977425675437
30-34	22.41579605768067	27.695055495035902	27.536962335669358	22.352186111614067
35-39	22.318176376360334	27.728587009613552	27.57928981261335	22.373946801412764
40-44	22.27510353477637	27.81069585066059	27.602930404380103	22.311270210182936
45-49	22.493175461144897	27.707225386723866	27.434840783846564	22.36475836828467
50-54	22.41689761427248	27.72594195456376	27.502850360133152	22.35431007103061
55-59	22.35242687098892	27.712667208210355	27.548802419994505	22.386103500806215
60-64	22.364131734295313	27.680171288751954	27.55924741917493	22.3964495577778
65-69	22.31799828038441	27.598976014099925	27.628441003894032	22.45458470162163
70-74	22.204788605023694	27.626907399656936	27.69719264674696	22.47111134857241
75-79	22.198644293854404	27.50548881903569	27.71677660795106	22.579090279158844
80-84	22.028031646995306	27.37300190331829	27.94692937801959	22.65203707166681
85-89	21.07093068009906	27.50434438748671	28.40545066032381	23.019274272090414
90-94	21.733219870500246	27.26907167279692	28.707324900189242	22.29038355651359
95-99	22.25229115758289	27.619520744473373	27.74578100328446	22.38240709465928
100-104	22.26905780689157	27.616258853157337	27.69363484727997	22.421048492671122
105-109	22.17476584637548	27.624386048685697	27.8329692867451	22.367878818193724
110-114	22.19304970863652	27.56811533595906	27.81477173824749	22.42406321715693
115-119	22.213526067620105	27.643179208384694	27.718756785598366	22.424537938396842
120-124	22.19821311961323	27.675006313125756	27.730529314539798	22.396251252721214
125-129	22.180942294669936	27.70965673652685	27.73865206419274	22.37074890461048
130-134	22.25302946476723	27.71177299996721	27.690427735518252	22.3447697997473
135-139	22.24821329539943	27.74381233413381	27.683284023285882	22.324690347180876
140-144	22.218384999886492	27.81676522830295	27.7019701967504	22.262879575060165
145-149	22.171723918631216	27.875393140749537	27.686599268026995	22.266283672592245
150-154	22.09540275288962	27.990400418412936	27.725031418912355	22.18916540978509
155-159	22.0497657916476	28.074264407791304	27.73479937903999	22.1411704215211
160-164	21.98016114894072	28.218256711566898	27.720895045052867	22.08068709443952
165-169	21.887977781783995	28.343239116011198	27.758384508663802	22.010398593541
170-174	21.738635097720287	28.582228273649292	27.785580313092762	21.89355631553766
175-178	21.81230933393104	28.6903392555522	27.600733442407805	21.896617968108956
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.5
11	7.5
12	16.0
13	23.5
14	50.5
15	118.0
16	238.5
17	464.5
18	909.0
19	1612.0
20	2814.5
21	4735.5
22	7531.5
23	11388.5
24	16428.0
25	22802.0
26	30637.0
27	39742.0
28	49996.5
29	61207.0
30	73072.0
31	86038.0
32	99839.5
33	114156.0
34	129350.5
35	145977.0
36	162819.5
37	179081.0
38	196177.5
39	213568.5
40	229286.0
41	242731.5
42	254387.5
43	263555.0
44	268932.0
45	269811.5
46	266672.5
47	260558.5
48	251209.0
49	238797.5
50	224426.5
51	207614.0
52	189823.5
53	171790.5
54	153418.5
55	135888.5
56	120048.0
57	105295.0
58	91369.5
59	78992.0
60	68021.0
61	58542.5
62	49946.0
63	42293.5
64	35577.0
65	29823.5
66	24676.0
67	19948.5
68	15781.5
69	12268.5
70	9574.0
71	7214.5
72	5115.0
73	3607.5
74	2538.0
75	1644.5
76	1040.0
77	679.0
78	389.0
79	210.0
80	129.0
81	76.5
82	33.5
83	15.0
84	8.5
85	4.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	534.0
92-93	1292.0
94-95	2129.0
96-97	2960.0
98-99	4041.0
100-101	5002.0
102-103	6196.0
104-105	7562.0
106-107	9082.0
108-109	11154.0
110-111	13772.0
112-113	14795.0
114-115	16148.0
116-117	17980.0
118-119	20584.0
120-121	23359.0
122-123	26539.0
124-125	29555.0
126-127	33805.0
128-129	38385.0
130-131	43218.0
132-133	55186.0
134-135	74432.0
136-137	80951.0
138-139	85545.0
140-141	92206.0
142-143	92918.0
144-145	98019.0
146-147	115645.0
148-149	117539.0
150-151	124959.0
152-153	132488.0
154-155	138306.0
156-157	151326.0
158-159	169574.0
160-161	181963.0
162-163	199880.0
164-165	223955.0
166-167	256078.0
168-169	269718.0
170-171	300850.0
172-173	320661.0
174-175	452030.0
176-177	1662121.0
178-179	339704.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.21449417401931
#Duplication Level	Percentage of total
1	91.21911929917471
2	7.023320201681517
3	1.0724560612826042
4	0.2826229423877947
5	0.11873229697324712
6	0.07494905841020329
7	0.038831844802754784
8	0.03068505262720534
9	0.02133493915178303
>10	0.11306702227337664
>50	0.0048812812348687385
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.6490368140872597E-5	1.6490368140872597E-5	0.0	0.0	0.0
2	0.0	4.947110442261779E-5	3.2980736281745194E-5	0.0	0.0	0.0
3	0.0	4.947110442261779E-5	3.2980736281745194E-5	0.0	0.0	0.0
4	0.0	6.596147256349039E-5	3.2980736281745194E-5	0.0	0.0	0.0
5	0.0	6.596147256349039E-5	3.2980736281745194E-5	1.6490368140872597E-5	0.0	0.0
6	0.0	6.596147256349039E-5	3.2980736281745194E-5	1.6490368140872597E-5	0.0	0.0
7	0.0	8.245184070436299E-5	3.2980736281745194E-5	1.6490368140872597E-5	0.0	0.0
8	0.0	8.245184070436299E-5	3.2980736281745194E-5	1.6490368140872597E-5	0.0	0.0
9	3.2980736281745194E-5	8.245184070436299E-5	3.2980736281745194E-5	1.6490368140872597E-5	0.0	0.0
10-14	3.2980736281745194E-5	8.245184070436299E-5	3.2980736281745194E-5	1.9788441769047116E-5	0.0	0.0
15-19	3.2980736281745194E-5	1.1543257698610819E-4	3.2980736281745194E-5	3.2980736281745194E-5	0.0	0.0
20-24	5.606725167896683E-5	1.352210187551553E-4	4.287495716626875E-5	3.2980736281745194E-5	6.596147256349039E-6	0.0
25-29	7.255761981983942E-5	1.6160560778055147E-4	6.596147256349039E-5	3.2980736281745194E-5	1.6490368140872597E-5	0.0
30-34	1.1213450335793366E-4	2.1107671220316928E-4	9.234606158888655E-5	3.2980736281745194E-5	2.3086515397221638E-5	0.0
35-39	2.704420375103106E-4	2.3416322760039086E-4	1.1543257698610819E-4	3.2980736281745194E-5	6.266339893531587E-5	0.0
40-44	4.683264552007817E-4	2.836343320230087E-4	1.1543257698610819E-4	3.2980736281745194E-5	1.4511523963967884E-4	0.0
45-49	5.90355179443239E-4	3.0342277379205574E-4	1.1543257698610819E-4	4.287495716626875E-5	2.2756708034404183E-4	3.2980736281745196E-6
50-54	7.948357443900593E-4	3.5949002547102266E-4	1.385190923833298E-4	6.266339893531587E-5	2.8363433202300864E-4	1.6490368140872597E-5
55-59	8.937779532352948E-4	3.957688353809424E-4	1.4841331326785338E-4	8.245184070436298E-5	3.4299965733015E-4	1.6490368140872597E-5
60-64	0.001022402824734101	4.155572771499895E-4	1.517113868960279E-4	1.3192294512698078E-4	4.3534571891903657E-4	1.6490368140872597E-5
65-69	0.0011576238434892563	4.2874957166268754E-4	1.6490368140872598E-4	1.4511523963967886E-4	4.782206760853053E-4	1.6490368140872597E-5
70-74	0.004317178379280447	4.518360870599092E-4	1.6490368140872598E-4	1.6490368140872598E-4	6.035474739559371E-4	3.2980736281745194E-5
75-79	0.05188199624481338	4.683264552007817E-4	1.6490368140872598E-4	1.682017550369005E-4	7.288742718265689E-4	3.2980736281745194E-5
80-84	0.0643948875901075	5.705667376741919E-4	1.7149982866507501E-4	2.2426900671586734E-4	9.102683213761675E-4	4.94711044226178E-5
85-89	0.0645103201670936	5.936532530714135E-4	1.813940495495986E-4	2.47355522113089E-4	0.0010158066774777518	4.94711044226178E-5
90-94	0.06457628163965709	6.035474739559371E-4	1.813940495495986E-4	2.803362583948342E-4	0.001022402824734101	4.94711044226178E-5
95-99	0.06467522384850231	6.530185783785549E-4	1.813940495495986E-4	3.1001892104840477E-4	0.001022402824734101	4.94711044226178E-5
100-104	0.06476097376283488	7.222781245702197E-4	1.813940495495986E-4	3.199131419329284E-4	0.001022402824734101	4.94711044226178E-5
105-109	0.06481044486725748	8.014318916464083E-4	1.978844176904712E-4	3.331054364456265E-4	0.00102899897199045	4.94711044226178E-5
110-114	0.06489619478159	8.080280389027572E-4	2.1437478583134377E-4	3.693842463555462E-4	0.0010421912665031484	4.94711044226178E-5
115-119	0.06503801194760153	8.080280389027572E-4	2.2756708034404183E-4	4.3204764529086205E-4	0.0010817681500412423	4.94711044226178E-5
120-124	0.06562836712704476	8.179222597872809E-4	2.4075937485673994E-4	4.6502838157260726E-4	0.001104854665438464	4.94711044226178E-5
125-129	0.06647927012311379	8.44306848812677E-4	2.47355522113089E-4	4.782206760853053E-4	0.0011279411808356858	4.94711044226178E-5
130-134	0.06760061515669313	8.574991433253751E-4	2.47355522113089E-4	4.782206760853053E-4	0.001180710358886478	6.266339893531587E-5
135-139	0.0691704982037042	8.937779532352948E-4	2.770381847666596E-4	4.8811489696982887E-4	0.0012268833896809214	6.596147256349039E-5
140-144	0.07061835252647282	9.399509840297381E-4	2.803362583948342E-4	4.980091178543525E-4	0.0012664602732190154	6.596147256349039E-5
145-149	0.07235643732852079	9.564413521706106E-4	2.803362583948342E-4	5.112014123670505E-4	0.0013291236721543313	6.596147256349039E-5
150-154	0.07411760864596598	9.729317203114832E-4	2.803362583948342E-4	5.342879277642721E-4	0.001352210187551553	6.596147256349039E-5
155-159	0.07925270928503371	9.828259411960067E-4	2.803362583948342E-4	5.606725167896684E-4	0.0014016812919741709	6.596147256349039E-5
160-164	0.0864128271318006	9.89422088452356E-4	2.803362583948342E-4	5.90355179443239E-4	0.0014808350590503592	8.245184070436299E-5
165-167	0.09445133192153794	9.89422088452356E-4	2.803362583948342E-4	5.936532530714135E-4	0.0015006235008194063	8.245184070436299E-5
>>END_MODULE
