##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293516
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4039014
Total Bases	703.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.5800603810732	39.0	39.0	39.0	38.0	39.0
2	40.37454240069482	41.0	40.0	41.0	39.0	41.0
3	40.38725119546503	41.0	40.0	41.0	39.0	41.0
4	40.38775775473915	41.0	40.0	41.0	39.0	41.0
5	40.37334681187042	41.0	40.0	41.0	39.0	41.0
6	40.387148695201354	41.0	40.0	41.0	39.0	41.0
7	40.40492159720169	41.0	40.0	41.0	39.0	41.0
8	40.38126310035073	41.0	40.0	41.0	39.0	41.0
9	40.379720397106816	41.0	40.0	41.0	39.0	41.0
10-14	40.31137218142844	41.0	40.0	41.0	39.0	41.0
15-19	40.23831643069324	41.0	40.0	41.0	38.4	41.0
20-24	40.13539403428659	41.0	40.0	41.0	38.0	41.0
25-29	39.97801468378174	41.0	40.0	41.0	38.0	41.0
30-34	39.73623032750073	41.0	39.6	41.0	37.0	41.0
35-39	39.375635538772585	40.2	38.8	41.0	35.6	41.0
40-44	38.93780482068148	40.0	37.6	41.0	35.0	41.0
45-49	38.421379920941106	39.4	36.4	41.0	35.0	41.0
50-54	37.82379041023379	38.0	35.0	40.8	35.0	41.0
55-59	37.03750776798496	36.6	35.0	39.4	35.0	41.0
60-64	36.13843720274304	35.4	34.8	37.6	34.0	40.2
65-69	35.662222611756235	35.0	35.0	36.4	33.8	39.0
70-74	35.37978204581613	35.0	35.0	36.0	34.0	37.2
75-79	35.1538061516994	35.0	35.0	35.0	34.0	36.4
80-84	35.05260496744997	35.0	35.0	35.0	34.0	36.0
85-89	34.93616110268496	35.0	34.8	35.0	33.8	36.0
90-94	39.832094515476555	40.6	39.8	40.6	37.8	40.6
95-99	40.15031241608626	41.0	40.0	41.0	38.0	41.0
100-104	40.04272130621437	41.0	40.0	41.0	38.0	41.0
105-109	39.93567968780586	41.0	40.0	41.0	38.0	41.0
110-114	39.779185918558554	41.0	40.0	41.0	37.2	41.0
115-119	39.57332027371355	40.6	39.2	41.0	36.4	41.0
120-124	39.273187407597206	40.0	38.6	41.0	35.2	41.0
125-129	38.39489315721094	39.4	37.4	40.4	34.4	40.6
130-134	38.49433441348687	39.8	36.8	40.8	34.8	41.0
135-139	38.26157024397408	39.0	35.6	41.0	35.0	41.0
140-144	37.58703092537484	37.6	35.0	40.6	35.0	41.0
145-149	36.80220267193408	36.2	35.0	39.0	34.8	41.0
150-154	36.08643433926222	35.2	35.0	37.2	34.0	39.6
155-159	35.510972922395375	35.0	35.0	36.2	34.0	38.2
160-164	35.11290008397977	35.0	35.0	35.4	34.0	36.6
165-169	34.88575998593525	35.0	35.0	35.0	34.0	36.0
170-174	34.764100656636714	35.0	35.0	35.0	34.0	36.0
175-178	34.59945365596639	35.0	34.75	35.0	33.75	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	8.0
30	102.0
31	722.0
32	3949.0
33	15199.0
34	47221.0
35	133379.0
36	429995.0
37	1275560.0
38	1668981.0
39	458540.0
40	5357.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.342708888852577	27.616542057046594	28.526813722358973	22.513935331741855
2	22.09625913651203	28.107429189401174	28.97845860400583	20.817853070080965
3	20.575962351207497	26.998668486912898	29.844981968371485	22.58038719350812
4	21.32869556777966	26.382676564131742	30.18687234062571	22.101755527462892
5	22.172342061701197	26.938431013113593	28.475365522377494	22.413861402807715
6	22.620198890125167	26.992503615981523	27.82290430288184	22.56439319101147
7	22.496133957446048	27.497428827926818	27.664103169734	22.342334044893132
8	22.379125202338987	27.625628433077974	27.690099613420504	22.305146751162535
9	22.80784369650613	27.504732590676834	27.21976205083716	22.46766166197988
10-14	22.54009022994226	27.655977424193136	27.39558714082199	22.408345205042618
15-19	22.528240803324774	27.631530863720698	27.478770808915243	22.36145752403928
20-24	22.427533056335037	27.54290527341574	27.626891117485602	22.40267055276362
25-29	22.3589717688525	27.6633604142001	27.559874761513576	22.417793055433826
30-34	22.407443994994818	27.719537491080743	27.50311338361293	22.36990513031151
35-39	22.322933270347665	27.710703651930892	27.542197179806756	22.424165897914687
40-44	22.302388652279987	27.792169078889057	27.556106515104922	22.34933575372603
45-49	22.487988405090945	27.694991896537125	27.40423776694015	22.41278193143178
50-54	22.41968955789705	27.732916994098066	27.48906044891154	22.358332999093346
55-59	22.39962030337107	27.742874869955887	27.492665289102735	22.364839537570308
60-64	22.445745422026267	27.734551056272643	27.43739437397345	22.38230914772764
65-69	22.409182042944146	27.69369455020458	27.486094378479496	22.411029028371775
70-74	22.349746745121458	27.78164670882547	27.499280765058003	22.369325780995062
75-79	22.43478730204946	27.729723145302295	27.413641051999328	22.42184850064892
80-84	22.39613925576886	27.758992665041514	27.42044469268985	22.424423386499775
85-89	22.386131862875445	27.812396787928932	27.39640912361284	22.40506222558278
90-94	21.377050420764387	27.24470523020309	29.24662730120265	22.131617047829877
95-99	22.32303875273167	27.56110108394902	27.720685288208873	22.39517487511044
100-104	22.370484154499792	27.654624081551827	27.55121215207893	22.423679611869446
105-109	22.19065957844937	27.691536940794364	27.76317546631764	22.354628014438628
110-114	22.268783017704674	27.548541839140494	27.748625138072175	22.434050005082657
115-119	22.233280557626507	27.592326907465896	27.688058081718907	22.486334453188682
120-124	22.172247957093195	27.66835927753233	27.719706747785576	22.439686017588897
125-129	22.189434005666065	27.663672401176942	27.68814178195647	22.45875181120052
130-134	22.275380040366404	27.661316960236743	27.641163739794088	22.422139259602762
135-139	22.303161808724838	27.64103556176955	27.64869187255573	22.407110756949884
140-144	22.33256609067853	27.66673722618618	27.647065568063343	22.353631115071952
145-149	22.288944947523213	27.68312126670794	27.65158371158974	22.37635007417911
150-154	22.20206288823369	27.7878739702676	27.7083196444686	22.30174349703011
155-159	22.242878180269607	27.76007245662793	27.665554891159832	22.33149447194263
160-164	22.174762602254063	27.853344921701336	27.690555855470468	22.281336620574134
165-169	22.111229245521947	27.877563162784615	27.729994672755115	22.281212918938323
170-174	22.165020161008126	27.913982517090396	27.70229750197057	22.218699819930908
175-178	22.083340385188883	28.008555019355153	27.68336477974575	22.224739815710212
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	1.0
10	2.0
11	2.0
12	5.0
13	11.0
14	36.0
15	77.5
16	136.0
17	264.5
18	535.0
19	966.0
20	1736.0
21	3015.5
22	4937.0
23	7544.0
24	10848.5
25	15129.0
26	20245.0
27	26470.0
28	33343.0
29	40384.0
30	48212.0
31	56790.5
32	66023.0
33	76010.5
34	86014.5
35	96299.0
36	107388.0
37	118782.0
38	130034.0
39	140906.0
40	151027.5
41	160484.5
42	168624.5
43	174572.0
44	178171.5
45	179276.5
46	178256.5
47	174103.5
48	167959.5
49	160314.5
50	150520.5
51	139190.5
52	127436.0
53	115329.5
54	103268.5
55	91850.5
56	80847.0
57	70927.0
58	61804.0
59	53458.5
60	45953.0
61	39400.0
62	33629.5
63	28453.0
64	24054.0
65	20031.0
66	16227.0
67	13082.5
68	10459.5
69	8068.5
70	6068.5
71	4504.5
72	3257.0
73	2269.0
74	1533.0
75	1016.5
76	642.0
77	401.0
78	247.5
79	146.0
80	76.5
81	37.0
82	15.0
83	6.5
84	2.0
85	0.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	202.0
92-93	396.0
94-95	635.0
96-97	805.0
98-99	930.0
100-101	1075.0
102-103	1236.0
104-105	1365.0
106-107	1443.0
108-109	1695.0
110-111	1764.0
112-113	1934.0
114-115	2113.0
116-117	2216.0
118-119	2230.0
120-121	2381.0
122-123	2545.0
124-125	2700.0
126-127	2848.0
128-129	2973.0
130-131	3144.0
132-133	3344.0
134-135	28188.0
136-137	26856.0
138-139	27682.0
140-141	26855.0
142-143	25646.0
144-145	25095.0
146-147	24511.0
148-149	24171.0
150-151	24837.0
152-153	24579.0
154-155	24054.0
156-157	24339.0
158-159	25527.0
160-161	26044.0
162-163	26054.0
164-165	27681.0
166-167	28839.0
168-169	28180.0
170-171	28837.0
172-173	29756.0
174-175	29727.0
176-177	57732.0
178-179	3383850.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.67007525117933
#Duplication Level	Percentage of total
1	93.79345666535477
2	5.1828019966604435
3	0.6411029706166413
4	0.16590083276355347
5	0.06613348862187905
6	0.03719447276727568
7	0.022591045151094988
8	0.00980039095642928
9	0.01873746706819407
>10	0.06038880159731362
>50	0.0018918684423963649
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.4758517796670178E-5	0.0	0.0
2	2.4758517796670178E-5	0.0	0.0	2.4758517796670178E-5	0.0	0.0
3	2.4758517796670178E-5	0.0	0.0	2.4758517796670178E-5	0.0	0.0
4	4.9517035593340355E-5	0.0	0.0	2.4758517796670178E-5	0.0	0.0
5	4.9517035593340355E-5	0.0	0.0	2.4758517796670178E-5	0.0	0.0
6	4.9517035593340355E-5	2.4758517796670178E-5	0.0	4.9517035593340355E-5	0.0	0.0
7	4.9517035593340355E-5	2.4758517796670178E-5	0.0	7.427555339001054E-5	0.0	0.0
8	4.9517035593340355E-5	2.4758517796670178E-5	0.0	7.427555339001054E-5	0.0	0.0
9	4.9517035593340355E-5	2.4758517796670178E-5	0.0	7.427555339001054E-5	0.0	0.0
10-14	8.417896050867861E-5	4.951703559334036E-5	9.90340711866807E-6	7.427555339001054E-5	0.0	0.0
15-19	9.903407118668072E-5	7.427555339001054E-5	2.475851779667018E-5	7.427555339001054E-5	0.0	0.0
20-24	1.2874429254268493E-4	1.237925889833509E-4	3.961362847467229E-5	8.417896050867861E-5	0.0	0.0
25-29	1.7330962457669125E-4	1.9806814237336145E-4	8.417896050867861E-5	9.903407118668072E-5	4.951703559334035E-6	0.0
30-34	2.525368815260358E-4	2.673919922040379E-4	9.903407118668072E-5	9.903407118668072E-5	2.475851779667018E-5	0.0
35-39	7.378038303407713E-4	3.268124349160463E-4	9.903407118668072E-5	9.903407118668072E-5	2.475851779667018E-5	1.980681423733614E-5
40-44	0.0012082156684775046	3.614743598313846E-4	1.0398577474601475E-4	1.3369599610201896E-4	5.942044271200843E-5	3.961362847467228E-5
45-49	0.001426090625088202	3.961362847467229E-4	1.4359940322068704E-4	2.079715494920295E-4	2.376817708480337E-4	7.922725694934457E-5
50-54	0.0017231928386482443	4.25846506102727E-4	1.7826132813602528E-4	2.6739199220403797E-4	3.119573242380442E-4	1.237925889833509E-4
55-59	0.0018766956489875996	4.951703559334036E-4	1.9806814237336145E-4	3.0700562067871026E-4	3.9118458118738884E-4	1.535028103393551E-4
60-64	0.002094570605598297	4.951703559334036E-4	2.129232530513635E-4	3.317641384753804E-4	5.050737630520717E-4	1.931164388140274E-4
65-69	0.002248073415937652	5.100254666114057E-4	2.624402886447039E-4	4.2089480254339296E-4	5.545907986454119E-4	1.9806814237336145E-4
70-74	0.005328033029843422	5.248805772894078E-4	3.1195732423804426E-4	4.456533203400632E-4	6.882867947474309E-4	2.1787495661069756E-4
75-79	0.06564968578965064	5.59542502204746E-4	3.3671584203471443E-4	4.456533203400632E-4	7.179970161034352E-4	2.228266601700316E-4
80-84	0.09250772589547845	5.793493164420822E-4	3.466192491533825E-4	4.654601345773993E-4	8.071276801714478E-4	2.228266601700316E-4
85-89	0.09270579403785181	5.942044271200843E-4	3.614743598313846E-4	4.951703559334036E-4	0.0011636503364434983	2.228266601700316E-4
90-94	0.09277511788768249	6.635282769507606E-4	3.7137776695005267E-4	4.951703559334036E-4	0.0012082156684775046	2.228266601700316E-4
95-99	0.09282463492327583	6.783833876287629E-4	3.7632947050938667E-4	5.298322808487419E-4	0.0012131673720368386	2.228266601700316E-4
100-104	0.09300289625141187	7.328521267814373E-4	4.0108798830605684E-4	5.694459093234141E-4	0.0012280224827148408	2.228266601700316E-4
105-109	0.09315639906175122	7.526589410187733E-4	4.2089480254339296E-4	5.892527235607502E-4	0.0012824912218675152	2.228266601700316E-4
110-114	0.09327523994717522	8.120793837307818E-4	4.2089480254339296E-4	6.486731662727587E-4	0.0013122014432235193	2.228266601700316E-4
115-119	0.09333961209344657	8.31886197968118E-4	4.3574991322139513E-4	6.93238498306765E-4	0.0013369599610201896	2.228266601700316E-4
120-124	0.09380012052446464	8.566447157647881E-4	4.456533203400632E-4	6.93238498306765E-4	0.0013617184788168597	2.228266601700316E-4
125-129	0.09464686183311075	8.764515300021243E-4	4.6050843101806525E-4	7.080936089847672E-4	0.0013815252930541957	2.228266601700316E-4
130-134	0.09547379632751954	9.309202691547986E-4	4.902186523740695E-4	7.576106445781074E-4	0.0013963804037321983	2.376817708480337E-4
135-139	0.096384909782437	9.507270833921348E-4	5.199288737300738E-4	7.873208659341116E-4	0.0014706559571222087	2.475851779667018E-4
140-144	0.0970385346522691	9.655821940701369E-4	5.199288737300738E-4	7.922725694934458E-4	0.0015152212891562148	2.475851779667018E-4
145-149	0.09783575892532188	9.903407118668072E-4	5.199288737300738E-4	7.922725694934458E-4	0.001559786621190221	2.921505100007081E-4
150-154	0.09841510824176396	9.903407118668072E-4	5.3973568796741E-4	8.417896050867861E-4	0.0015845451389868916	2.9710221356004214E-4
155-159	0.09888056837634136	0.0010051958225448094	5.694459093234141E-4	8.863549371207924E-4	0.0016538689888175682	2.9710221356004214E-4
160-164	0.09926680125396942	0.0010299543403414794	5.694459093234141E-4	9.309202691547986E-4	0.001658820692376902	2.9710221356004214E-4
165-167	0.09998314770221972	0.0010398577474601475	5.694459093234141E-4	9.408236762734667E-4	0.0016588206923769018	2.9710221356004214E-4
>>END_MODULE
