##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293517
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7793635
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.29079101086977	39.0	39.0	39.0	37.0	39.0
2	40.045396275293875	41.0	40.0	41.0	38.0	41.0
3	40.089181235713504	41.0	40.0	41.0	38.0	41.0
4	40.1077089188806	41.0	40.0	41.0	38.0	41.0
5	40.10879069907688	41.0	40.0	41.0	38.0	41.0
6	40.09878561159202	41.0	40.0	41.0	38.0	41.0
7	40.11817579345196	41.0	40.0	41.0	38.0	41.0
8	40.09941830737519	41.0	40.0	41.0	38.0	41.0
9	40.075228952857046	41.0	40.0	41.0	38.0	41.0
10-14	40.00271267515094	41.0	40.0	41.0	38.0	41.0
15-19	39.86668054123653	41.0	40.0	41.0	38.0	41.0
20-24	39.677574969831156	40.4	39.6	41.0	37.2	41.0
25-29	39.53754875613241	40.0	39.0	41.0	36.8	41.0
30-34	39.33731243508325	40.0	38.8	41.0	35.4	41.0
35-39	39.110682473582614	40.0	38.0	41.0	35.0	41.0
40-44	38.65602261332485	40.0	36.8	41.0	35.0	41.0
45-49	37.995122455696226	39.0	35.2	41.0	34.8	41.0
50-54	37.30792956046825	37.2	35.0	40.0	34.0	41.0
55-59	36.629113629262854	36.2	35.0	39.0	34.0	41.0
60-64	35.7818269908714	35.0	34.6	37.4	33.2	40.0
65-69	35.42801257692976	35.0	34.8	36.2	33.0	39.0
70-74	35.25814809135917	35.0	35.0	36.0	33.0	37.8
75-79	35.071010638809746	35.0	35.0	35.0	33.0	37.0
80-84	34.984194820517004	35.0	35.0	35.0	33.0	37.0
85-89	34.76076205775611	35.0	34.6	35.0	32.4	37.0
90-94	39.20502803334209	40.2	39.6	40.2	37.0	40.2
95-99	39.75056160210817	41.0	40.0	41.0	37.0	41.0
100-104	39.643198353120965	41.0	39.6	41.0	36.8	41.0
105-109	39.48012933814767	40.6	39.0	41.0	36.0	41.0
110-114	39.28828755934087	40.0	38.6	41.0	35.2	41.0
115-119	39.02190520458184	40.0	38.0	41.0	35.0	41.0
120-124	38.674606184082236	40.0	37.6	41.0	34.8	41.0
125-129	38.19827743434435	39.6	36.6	40.6	34.2	40.8
130-134	37.843848212884545	39.0	35.2	40.6	33.8	40.8
135-139	37.825468822910885	38.8	35.0	41.0	34.0	41.0
140-144	37.22770071104609	37.2	35.0	40.2	34.0	41.0
145-149	36.55637176935501	35.8	35.0	39.0	34.0	41.0
150-154	35.90860753723157	35.0	35.0	37.2	33.4	39.8
155-159	35.363635891839365	35.0	35.0	36.2	33.0	38.4
160-164	34.953860865552976	35.0	35.0	35.4	33.0	36.8
165-169	34.69656766536615	35.0	35.0	35.0	33.0	36.0
170-174	34.550565180185046	35.0	35.0	35.0	33.0	36.0
175-179	34.2703311131585	34.8	33.8	35.0	32.4	35.4
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	82.0
30	765.0
31	4904.0
32	23027.0
33	79706.0
34	211298.0
35	506461.0
36	1273993.0
37	2453498.0
38	2532691.0
39	698427.0
40	8780.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.938941200094693	26.84969208847989	28.597579948252644	22.61378676317277
2	23.03507926660666	27.832622389937427	27.890926377742865	21.241371965713046
3	20.24974225762433	26.677808237106305	30.3277738821487	22.74467562312066
4	21.317267231529318	25.408092629434147	30.678252189126127	22.596387949910408
5	22.525483936571316	26.382657129824526	28.15307619615237	22.93878273745178
6	22.38159729060958	26.92190486210863	28.035249277134483	22.661248570147304
7	22.573523137791287	27.0484696807074	27.453454004453636	22.92455317704768
8	22.83911165970693	27.263542621639324	27.05135665193456	22.845989066719188
9	22.870444920759056	27.43044805151896	27.14975746233946	22.54934956538252
10-14	22.855709819615623	27.25788929042738	27.04435863367992	22.84204225627708
15-19	22.857749945949482	27.15366321363523	27.176961712987584	22.8116251274277
20-24	22.84409521359417	26.999583634594128	27.20512572118145	22.95119543063025
25-29	22.972872093702104	26.913313235736595	26.959892271064785	23.153922399496512
30-34	22.947415422970156	27.03564382986886	26.998526361575827	23.01841438558516
35-39	22.927101410317523	27.01864790947998	27.031815064472482	23.022435615730018
40-44	22.96137040033309	27.001667386270977	26.936678456201758	23.100283757194173
45-49	23.066407395265497	26.915561223998814	26.93186683748982	23.086164543245864
50-54	23.097668802811526	26.995754869197746	26.874345539661533	23.032230788329194
55-59	23.042300543969535	26.884081689737844	26.906281856925556	23.16733590936707
60-64	23.01430333855768	27.004744256049968	26.931533231925798	23.049419173466553
65-69	22.982107835432373	26.975109817177735	26.96523509248252	23.077547254907373
70-74	22.970747282878914	26.996591449304464	26.903810609555105	23.12885065826152
75-79	22.92489961359494	26.977319312490255	26.981191703229623	23.11658937068518
80-84	22.935464645188027	27.04512592647718	26.918907544425675	23.100501883909114
85-89	22.857365016452526	27.012635310737544	26.885742018968045	23.244257653841885
90-94	21.93625542346646	26.893073537385	28.878443922375784	22.29222711677276
95-99	22.539269850195613	27.112874319910222	27.525690211087124	22.822165618807038
100-104	22.724949289622447	27.32337203825382	27.10688579951445	22.844792872609286
105-109	22.73663301766722	27.241510674955784	27.19732869761269	22.82452760976431
110-114	22.743441157519793	27.088100336549726	27.185769774246022	22.98268873168446
115-119	22.817296372815335	27.003531577785562	26.99775082944783	23.18142121995127
120-124	22.785755867281228	27.13949151958334	26.99727675179917	23.07747586133626
125-129	22.741283920225452	27.11734522937894	27.04234772942653	23.099023120969076
130-134	22.869351131043096	27.09825754885683	26.908113011687906	23.124278308412165
135-139	22.974167290833215	27.030209946313562	26.929257907936428	23.066364854916795
140-144	22.973206855404197	27.14462035272019	26.91019060024423	22.97198219163138
145-149	22.878338474934946	27.110234907323584	26.961578788222994	23.049847829518477
150-154	22.851085819115717	27.240946621880767	27.015643381800764	22.892324177202756
155-159	22.736353256015114	27.290253164749966	27.085641283502465	22.88775229573245
160-164	22.69574175919648	27.374239239099012	27.079228300909424	22.85079070079508
165-169	22.590386441523297	27.449347391484807	27.2128776411719	22.747388525819993
170-174	22.546421892801007	27.58428571873482	27.21492431707584	22.654368071388337
175-179	22.448073869915568	27.627823227955435	27.246355914579535	22.67774698754946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	3.0
9	6.5
10	13.5
11	39.5
12	97.5
13	169.5
14	301.5
15	543.5
16	971.5
17	1655.5
18	2707.0
19	4287.0
20	6417.0
21	9511.0
22	13796.5
23	19064.5
24	25709.0
25	33559.0
26	42657.0
27	53245.5
28	64957.0
29	77976.0
30	92292.5
31	107831.0
32	124008.0
33	141207.0
34	159126.0
35	177731.0
36	197033.5
37	215549.5
38	234385.0
39	252381.5
40	268297.5
41	282782.5
42	295604.5
43	304420.5
44	307918.5
45	308240.0
46	305561.5
47	298451.0
48	287221.5
49	273388.0
50	258240.5
51	241471.5
52	223686.0
53	207323.0
54	191634.0
55	177006.5
56	164709.5
57	153762.5
58	143440.0
59	134061.5
60	125541.5
61	116680.5
62	107507.5
63	97467.5
64	86754.0
65	76030.0
66	65416.5
67	55079.5
68	45166.5
69	35970.5
70	27883.0
71	21179.5
72	15530.5
73	11077.5
74	7591.0
75	5001.0
76	3235.0
77	2022.5
78	1205.0
79	706.5
80	403.0
81	205.5
82	89.5
83	39.0
84	18.0
85	9.5
86	5.5
87	2.0
88	1.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1180.0
92-93	1927.0
94-95	3166.0
96-97	3526.0
98-99	4641.0
100-101	6229.0
102-103	6416.0
104-105	7205.0
106-107	8046.0
108-109	8998.0
110-111	9688.0
112-113	10500.0
114-115	11149.0
116-117	11971.0
118-119	12820.0
120-121	13022.0
122-123	13579.0
124-125	14199.0
126-127	14878.0
128-129	15262.0
130-131	16324.0
132-133	17517.0
134-135	68601.0
136-137	74987.0
138-139	79946.0
140-141	72246.0
142-143	81038.0
144-145	81253.0
146-147	75798.0
148-149	77383.0
150-151	78661.0
152-153	82278.0
154-155	77493.0
156-157	81112.0
158-159	88451.0
160-161	87985.0
162-163	84840.0
164-165	87729.0
166-167	85046.0
168-169	88828.0
170-171	92520.0
172-173	89661.0
174-175	95072.0
176-177	100685.0
178-179	5749779.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.23004707732473
#Duplication Level	Percentage of total
1	87.03605778957156
2	11.435525217686388
3	1.2204558160517363
4	0.20777464217851915
5	0.057402653739415645
6	0.019600766645358675
7	0.010382188696065836
8	0.004179384398566963
9	0.0021030019605915787
>10	0.006518539071772973
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.849294969548869E-4	1.2830983231829563E-5	1.2830983231829563E-5	2.5661966463659126E-5	0.0	0.0
2	6.15887195127819E-4	1.2830983231829563E-5	2.5661966463659126E-5	2.5661966463659126E-5	0.0	0.0
3	6.15887195127819E-4	3.849294969548869E-5	2.5661966463659126E-5	2.5661966463659126E-5	0.0	0.0
4	6.15887195127819E-4	3.849294969548869E-5	2.5661966463659126E-5	2.5661966463659126E-5	0.0	0.0
5	6.15887195127819E-4	3.849294969548869E-5	3.849294969548869E-5	2.5661966463659126E-5	1.2830983231829563E-5	0.0
6	6.287181783596486E-4	3.849294969548869E-5	5.132393292731825E-5	3.849294969548869E-5	1.2830983231829563E-5	0.0
7	6.287181783596486E-4	3.849294969548869E-5	5.132393292731825E-5	3.849294969548869E-5	1.2830983231829563E-5	0.0
8	6.287181783596486E-4	3.849294969548869E-5	6.415491615914782E-5	3.849294969548869E-5	1.2830983231829563E-5	0.0
9	6.287181783596486E-4	6.415491615914782E-5	7.698589939097738E-5	5.132393292731825E-5	1.2830983231829563E-5	0.0
10-14	6.287181783596486E-4	6.415491615914782E-5	9.238307926917286E-5	7.441970274461147E-5	2.3095769817293212E-5	0.0
15-19	6.543801448233077E-4	8.981688262280695E-5	1.5140560213558886E-4	1.1804504573283198E-4	3.0794359756390954E-5	0.0
20-24	7.108364710433579E-4	1.0264786585463649E-4	2.0016333841654118E-4	1.591041920746866E-4	4.362534298822051E-5	0.0
25-29	7.544618140315785E-4	1.1804504573283199E-4	2.2582530488020032E-4	1.8219996189197983E-4	7.185350609824556E-5	0.0
30-34	8.340139100689217E-4	1.4370701219649113E-4	2.7201684451478675E-4	2.1299432164837074E-4	1.2574363567192972E-4	0.0
35-39	9.289631859844605E-4	1.6167038872105251E-4	3.4900274390576414E-4	2.4892107469749356E-4	1.8733235518471163E-4	0.0
40-44	0.0010033828887290718	2.0016333841654118E-4	4.465182164676688E-4	2.9511261433207993E-4	2.617520579293231E-4	1.5397179878195477E-5
45-49	0.001057273018302756	2.232591082338344E-4	4.978421493949871E-4	3.387379573203005E-4	3.207745807957391E-4	4.6191539634586425E-5
50-54	0.001149656097571929	2.745830411611526E-4	5.235041158586463E-4	3.695323170766914E-4	3.6439992378395965E-4	1.0778025914736833E-4
55-59	0.0011958476372065153	3.0794359756390946E-4	5.696956554932327E-4	4.003266768330824E-4	4.465182164676688E-4	1.3344222561102745E-4
60-64	0.0013241574695248112	3.207745807957391E-4	5.953576219568917E-4	4.439520198213029E-4	5.029745426877189E-4	1.411408155501252E-4
65-69	0.0014319377286721793	3.207745807957391E-4	6.492477515305759E-4	5.106731326268167E-4	5.876590320177939E-4	1.411408155501252E-4
70-74	0.002181267149411026	3.207745807957391E-4	7.082702743969919E-4	5.748280487859644E-4	6.518139481769419E-4	1.4883940548922293E-4
75-79	0.014463084298918285	3.3360556402756863E-4	7.64726600617042E-4	6.004900152496237E-4	7.262336509215533E-4	1.6680278201378431E-4
80-84	0.019767412766956625	3.3873795732030043E-4	7.95520960373433E-4	6.518139481769419E-4	8.725068597644104E-4	1.6680278201378431E-4
85-89	0.01984696486299397	3.669661204303255E-4	8.237491234834579E-4	7.005716844578942E-4	0.0011214279344619038	1.796337652456139E-4
90-94	0.019944480335555875	4.054590701258142E-4	8.673744664716784E-4	7.159688643360897E-4	0.0011522222942182947	1.796337652456139E-4
95-99	0.020021466234946852	4.439520198213029E-4	8.827716463498741E-4	7.698589939097739E-4	0.0011650532774501242	1.796337652456139E-4
100-104	0.02009845213433783	4.824449695167916E-4	9.058674161671672E-4	8.109181402516284E-4	0.0011907152439137832	1.796337652456139E-4
105-109	0.020157474657204244	4.952759527486212E-4	9.520589558017535E-4	8.776392530571421E-4	0.0012009800304992474	1.796337652456139E-4
110-114	0.020221629573363393	5.004083460413529E-4	9.931181021436083E-4	9.366617759235581E-4	0.0012394729801947359	1.796337652456139E-4
115-119	0.020244725343180687	5.004083460413529E-4	0.0010444420350709264	9.9825049543634E-4	0.0012728335365974925	1.796337652456139E-4
120-124	0.02029348307946164	5.235041158586462E-4	0.0011034645579373423	0.0010316110518390968	0.0013472532393421042	1.796337652456139E-4
125-129	0.020521874580988204	5.465998856759394E-4	0.0011753180640355879	0.0010803687881200494	0.0014293715320258132	1.8989855183107755E-4
130-134	0.02068611116635562	5.59430868907769E-4	0.0012420391768411016	0.00111116314787644	0.001501225038124059	2.027295350629071E-4
135-139	0.0208760097181867	5.773942454323303E-4	0.0012702673399511268	0.0011393913109864653	0.0015782109375150361	2.181267149411026E-4
140-144	0.02110696741635963	5.9022522866416E-4	0.0012959293064147858	0.0011727518673892222	0.0016449320503205502	2.181267149411026E-4
145-149	0.021319961738008004	6.389829649451123E-4	0.0013421208460493723	0.0011958476372065153	0.0017860728658706752	2.181267149411026E-4
150-154	0.021438006783740835	6.595125381160395E-4	0.0013703490091593975	0.0012266419969629062	0.0018733235518471163	2.181267149411026E-4
155-159	0.021576581402644596	6.826083079333328E-4	0.0013908785823303247	0.0012677011433047606	0.0019477432545917275	2.2582530488020032E-4
160-164	0.021733119398072914	7.313660442142851E-4	0.001411408155501252	0.0012907969131220542	0.002011898170750876	2.3095769817293212E-4
165-168	0.02190569612254102	7.506125190620296E-4	0.001411408155501252	0.0013087602896466155	0.002027295350629071	2.3416544398088953E-4
>>END_MODULE
