##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293519
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7999939
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.27937188021059	39.0	39.0	39.0	37.0	39.0
2	40.04769073864188	41.0	40.0	41.0	38.0	41.0
3	40.08935418132563	41.0	40.0	41.0	38.0	41.0
4	40.100801768613486	41.0	40.0	41.0	38.0	41.0
5	40.10594755785013	41.0	40.0	41.0	38.0	41.0
6	40.09847725088904	41.0	40.0	41.0	38.0	41.0
7	40.11991966438744	41.0	40.0	41.0	38.0	41.0
8	40.09738286754437	41.0	40.0	41.0	38.0	41.0
9	40.07778409310371	41.0	40.0	41.0	38.0	41.0
10-14	39.997969884520366	41.0	40.0	41.0	38.0	41.0
15-19	39.86653003229149	41.0	40.0	41.0	38.0	41.0
20-24	39.68325918485128	40.4	39.6	41.0	37.2	41.0
25-29	39.54773705149502	40.0	39.0	41.0	37.0	41.0
30-34	39.35020062027972	40.0	38.8	41.0	35.6	41.0
35-39	39.114919226259104	40.0	38.0	41.0	35.0	41.0
40-44	38.64902984885261	40.0	36.8	41.0	35.0	41.0
45-49	37.99531723929395	39.0	35.2	41.0	34.8	41.0
50-54	37.30995803843004	37.2	35.0	40.0	34.0	41.0
55-59	36.61206751701482	36.0	35.0	39.0	34.0	41.0
60-64	35.783595374914725	35.0	34.6	37.2	33.0	39.8
65-69	35.42284159916719	35.0	34.8	36.2	33.0	39.0
70-74	35.234746864944846	35.0	35.0	36.0	33.0	37.6
75-79	35.05422401345811	35.0	35.0	35.0	33.0	37.0
80-84	34.972559640767265	35.0	35.0	35.0	33.0	36.6
85-89	34.783278672499875	35.0	34.6	35.0	32.4	37.2
90-94	39.15920740834262	40.2	39.6	40.2	36.6	40.2
95-99	39.73992583760397	41.0	40.0	41.0	37.0	41.0
100-104	39.62494811765769	41.0	39.2	41.0	36.6	41.0
105-109	39.469031754979376	40.4	39.0	41.0	36.0	41.0
110-114	39.26978423111719	40.0	38.6	41.0	35.2	41.0
115-119	39.00050568225191	40.0	38.0	41.0	35.0	41.0
120-124	38.63638503409893	40.0	37.6	41.0	34.8	41.0
125-129	38.18796524086114	39.6	36.6	40.6	34.2	40.8
130-134	37.833441816415515	39.0	35.2	40.6	34.0	40.8
135-139	37.781762372068556	38.8	35.0	41.0	34.0	41.0
140-144	37.174256363687604	36.8	35.0	40.2	34.0	41.0
145-149	36.50563713626635	35.8	35.0	39.0	34.0	41.0
150-154	35.87287643349546	35.0	35.0	37.2	33.4	39.6
155-159	35.32144114231666	35.0	35.0	36.0	33.0	38.2
160-164	34.941560873351875	35.0	35.0	35.4	33.0	36.8
165-169	34.69154611617638	35.0	35.0	35.0	33.0	36.0
170-174	34.54608992442423	35.0	34.8	35.0	33.0	36.0
175-179	34.27584383700681	34.8	33.8	35.0	32.4	35.4
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	8.0
29	75.0
30	804.0
31	5039.0
32	23380.0
33	81113.0
34	217449.0
35	522607.0
36	1316444.0
37	2523102.0
38	2588557.0
39	711568.0
40	9793.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.948492357254224	26.840967162374614	28.571642858776798	22.638897621594364
2	23.047675738527506	27.811699564209174	27.871275018472012	21.2693496787913
3	20.280579639419752	26.681678447798163	30.300831043836705	22.736910868945376
4	21.334462675277898	25.416468800574606	30.63785861367193	22.61120991047557
5	22.539159361090128	26.40002630020054	28.13906456036727	22.92174977834206
6	22.41113338489206	26.93908041048813	28.009188570062847	22.640597634556965
7	22.59939732040457	27.054893793565178	27.453546833294606	22.89216205273565
8	22.846786706748638	27.249745279307753	27.03008110436842	22.873386909575185
9	22.84303667815467	27.4229215997772	27.1778697312567	22.55617199081143
10-14	22.863661835421496	27.267155412060017	27.045336220688682	22.823846531829805
15-19	22.876071930048468	27.151082027000456	27.169597168178406	22.80324887477267
20-24	22.852964253852438	27.009833449980057	27.18789980773603	22.949302488431474
25-29	22.982295240001203	26.91427022131044	26.96661312042504	23.136821418263313
30-34	22.945199957149672	27.037558661384793	27.00017587634106	23.017065505124474
35-39	22.92687981745861	27.02530606795877	27.029333598668693	23.018480515913932
40-44	22.97917271619196	26.990445802149242	26.93635038967172	23.094031091987077
45-49	23.069735906736287	26.9126127086719	26.94392044739341	23.0737309371984
50-54	23.095656104377795	26.994660834288865	26.879417455558098	23.030265605775245
55-59	23.048333243540984	26.896370084821896	26.90084261892497	23.15445405271215
60-64	23.034258136218288	26.988675788652888	26.936000387002952	23.041065688125872
65-69	22.982262739753388	26.987933282991282	26.955313034261884	23.07449094299344
70-74	22.980622727248296	26.973725674658265	26.915952734139598	23.129698863953838
75-79	22.920552269211054	26.98233074027189	26.99232331646529	23.104793674051766
80-84	22.942072433302304	27.027376083742638	26.918332752287238	23.11221873066782
85-89	22.85829929453212	27.00251089414557	26.915997734482726	23.223192076839585
90-94	21.943195321547588	26.901528942428392	28.85637668779115	22.29889904823286
95-99	22.54499994694819	27.111104204365983	27.51535149226735	22.82854435641848
100-104	22.7239147166091	27.327737994710866	27.106151898320473	22.842195390359556
105-109	22.728182343623732	27.22547952684281	27.19862911394266	22.8477090155908
110-114	22.74296059934065	27.073643755743166	27.193801869987748	22.989593774928434
115-119	22.797729080861927	27.010670751262577	27.00693613633895	23.184664031536546
120-124	22.820813687508554	27.128412636839954	26.980209561958407	23.070564113693088
125-129	22.75186238088421	27.127403415829505	27.03006628455365	23.09066791873264
130-134	22.884067952850966	27.08899682831444	26.914432681542632	23.112502537291967
135-139	22.970387670799745	27.023650828352896	26.950602815555612	23.05535868529174
140-144	22.953554733727255	27.159570894163064	26.906871130207684	22.980003241901997
145-149	22.879218904901254	27.106074108644595	26.95894331693959	23.05576366951456
150-154	22.856533100656602	27.22897582763798	27.010406608769795	22.90408446293562
155-159	22.77312456618608	27.26263414548103	27.077399135794884	22.886842152538005
160-164	22.717647767624634	27.337664263980844	27.073774555303316	22.870913413091206
165-169	22.60652693109368	27.41466103262351	27.215500188043773	22.763311848239038
170-174	22.57040749322928	27.53929392310677	27.19436743338492	22.695931150279034
175-179	22.475086714835843	27.591189938747817	27.225211762011902	22.708511584404437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	2.0
9	3.5
10	10.0
11	33.0
12	82.5
13	174.5
14	328.5
15	578.0
16	1033.0
17	1701.5
18	2772.0
19	4380.0
20	6717.0
21	9990.0
22	14201.0
23	19524.0
24	26307.5
25	34394.0
26	43844.5
27	54539.0
28	66541.5
29	80430.5
30	95275.0
31	110549.5
32	126996.5
33	144354.5
34	163293.0
35	182822.0
36	201572.0
37	220658.5
38	240098.0
39	259049.5
40	276107.0
41	290541.5
42	302591.5
43	311149.0
44	315536.0
45	316837.5
46	314253.0
47	306638.5
48	295362.0
49	281307.0
50	264926.0
51	247639.5
52	230173.0
53	212855.5
54	196560.5
55	182341.5
56	169967.5
57	157992.0
58	146908.0
59	137475.5
60	128440.0
61	119454.0
62	110137.0
63	99846.0
64	89109.5
65	78439.5
66	67382.5
67	56304.0
68	46050.0
69	36954.5
70	28757.0
71	21606.0
72	15966.5
73	11430.5
74	7795.5
75	5201.0
76	3404.0
77	2108.0
78	1249.5
79	707.0
80	384.5
81	183.0
82	89.0
83	48.5
84	29.5
85	14.0
86	6.5
87	4.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1023.0
92-93	1966.0
94-95	2841.0
96-97	3635.0
98-99	4685.0
100-101	5704.0
102-103	6700.0
104-105	8749.0
106-107	8410.0
108-109	10621.0
110-111	10794.0
112-113	12497.0
114-115	11605.0
116-117	12356.0
118-119	13214.0
120-121	14183.0
122-123	16489.0
124-125	14776.0
126-127	15607.0
128-129	16559.0
130-131	17442.0
132-133	18833.0
134-135	87493.0
136-137	86074.0
138-139	72097.0
140-141	74231.0
142-143	78351.0
144-145	92151.0
146-147	127843.0
148-149	78204.0
150-151	84329.0
152-153	79804.0
154-155	123971.0
156-157	74492.0
158-159	85048.0
160-161	87071.0
162-163	89286.0
164-165	85165.0
166-167	90176.0
168-169	94683.0
170-171	90476.0
172-173	96045.0
174-175	154937.0
176-177	100469.0
178-179	5738854.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.12373547221885
#Duplication Level	Percentage of total
1	86.85770680703074
2	11.524170627442148
3	1.2837927620429799
4	0.2331229071716759
5	0.05838949851372514
6	0.020615532710263357
7	0.010372023626850047
8	0.005218271715041115
9	0.0010501325702365913
>10	0.00556143717632275
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.62502001577762E-4	0.0	0.0	0.0	1.2500095313226764E-5	0.0
2	6.250047656613381E-4	1.2500095313226764E-5	2.5000190626453527E-5	1.2500095313226764E-5	3.750028593968029E-5	0.0
3	6.37504860974565E-4	2.5000190626453527E-5	5.0000381252907055E-5	2.5000190626453527E-5	3.750028593968029E-5	0.0
4	6.37504860974565E-4	2.5000190626453527E-5	5.0000381252907055E-5	3.750028593968029E-5	3.750028593968029E-5	0.0
5	6.37504860974565E-4	2.5000190626453527E-5	5.0000381252907055E-5	3.750028593968029E-5	5.0000381252907055E-5	0.0
6	6.750051469142452E-4	2.5000190626453527E-5	5.0000381252907055E-5	3.750028593968029E-5	5.0000381252907055E-5	0.0
7	6.750051469142452E-4	3.750028593968029E-5	5.0000381252907055E-5	3.750028593968029E-5	5.0000381252907055E-5	0.0
8	6.87505242227472E-4	3.750028593968029E-5	7.500057187936058E-5	3.750028593968029E-5	5.0000381252907055E-5	0.0
9	6.87505242227472E-4	5.0000381252907055E-5	7.500057187936058E-5	6.250047656613382E-5	5.0000381252907055E-5	0.0
10-14	7.075053947286347E-4	5.5000419378197766E-5	1.0000076250581411E-4	9.750074344316876E-5	5.0000381252907055E-5	0.0
15-19	7.450056806683151E-4	8.750066719258734E-5	1.8750142969840145E-4	1.0250078156845946E-4	6.500049562877916E-5	0.0
20-24	7.8000594754535E-4	1.1500087688168623E-4	2.3250177282601778E-4	1.1250085781904085E-4	1.2000091500697693E-4	0.0
25-29	8.175062334850302E-4	1.2500095313226764E-4	2.625020015777621E-4	1.1500087688168623E-4	1.7250131532252933E-4	0.0
30-34	8.650065956752921E-4	1.2500095313226764E-4	3.2250245908125046E-4	1.4250108657078508E-4	2.4250184907659923E-4	0.0
35-39	0.0010050076631834318	1.475011246960758E-4	4.1000312627383783E-4	1.625012390719479E-4	3.2500247814389586E-4	1.0000076250581411E-5
40-44	0.001090008311313374	1.850014106357561E-4	4.575034884640995E-4	1.950014868863375E-4	4.3750333596293667E-4	3.750028593968029E-5
45-49	0.0011800089975686065	2.175016584501457E-4	4.8250367909055306E-4	2.3500179188866316E-4	4.975037934664252E-4	8.5000648129942E-5
50-54	0.0012775097410117752	2.3000175376337246E-4	5.200039650302332E-4	2.8500217314157017E-4	5.400041175313963E-4	1.5000114375872116E-4
55-59	0.0013400102175779092	2.5250192532718063E-4	5.275040222181694E-4	3.3500255439447725E-4	6.575050134757278E-4	1.8750142969840147E-4
60-64	0.001482511304148694	2.8750219220421557E-4	5.600042700325588E-4	4.2750325971235533E-4	7.550057569188966E-4	2.0000152501162822E-4
65-69	0.0016425125241579966	3.0250230658008766E-4	6.100046512854661E-4	4.925037553411345E-4	8.72506652863228E-4	2.2000167751279106E-4
70-74	0.002347517899823986	3.2500247814389586E-4	6.250047656613381E-4	5.60004270032559E-4	9.400071675546526E-4	2.3500179188866316E-4
75-79	0.014860113308363976	3.4500263064505865E-4	6.550049944130824E-4	6.350048419119195E-4	9.600073200558154E-4	2.600019825151167E-4
80-84	0.02035765522712111	3.650027831462215E-4	6.750051469142452E-4	6.850052231648266E-4	0.0010675081397495654	2.7500209689098877E-4
85-89	0.020432655799000467	3.850029356473843E-4	7.125054328539256E-4	7.575057759815418E-4	0.0013225100841393917	2.7500209689098877E-4
90-94	0.020547656675882153	3.9250299283532034E-4	7.650058331694779E-4	8.22506271610321E-4	0.0013475102747658452	2.7500209689098877E-4
95-99	0.02061515719057358	4.0000305002325644E-4	7.875060047332861E-4	8.825067291138095E-4	0.00138251054164288	2.7500209689098877E-4
100-104	0.020660157533701193	4.150031643991286E-4	8.475064622367745E-4	9.375071484920072E-4	0.001445011018209014	2.7500209689098877E-4
105-109	0.020720157991204684	4.2500324064971E-4	8.825067291138095E-4	9.725074153690422E-4	0.0014500110563343046	2.7500209689098877E-4
110-114	0.020792658544021396	4.350033169002914E-4	8.950068244270362E-4	9.975076059954957E-4	0.0014800112850860488	2.7500209689098877E-4
115-119	0.020877659192151343	4.500034312761634E-4	9.375071484920073E-4	0.0010450079681857574	0.0014925113803992758	2.7500209689098877E-4
120-124	0.02103266037403535	4.700035837773263E-4	0.0010100077013087225	0.001070008158812211	0.0015150115519630837	2.7500209689098877E-4
125-129	0.021145161231854392	4.8500369815319847E-4	0.0010725081778748563	0.0011075084447518912	0.00159251214290509	2.8750219220421557E-4
130-134	0.02128766231842518	4.875037172158437E-4	0.001112508482877182	0.001170008921318025	0.0016750127719723864	3.200024400186051E-4
135-139	0.021465163671872997	4.875037172158437E-4	0.0011250085781904088	0.001232509397884159	0.0017375132485385203	3.275024972065412E-4
140-144	0.021645165044383463	5.050038506543613E-4	0.0011450087306915716	0.0012900098363250018	0.0018075137822925898	3.525026878329947E-4
145-149	0.02175516588313986	5.200039650302332E-4	0.001197509131007124	0.0013000099125755834	0.0018975144685478228	3.800028975220936E-4
150-154	0.021892666931585352	5.475041747193323E-4	0.0012200093025709323	0.0013275101222646822	0.0019400147926127939	3.8750295471002964E-4
155-159	0.022102668532847564	5.775044034710765E-4	0.001247509512260031	0.0013525103128911357	0.00198501513574041	3.8750295471002964E-4
160-164	0.022427671010991457	5.925045178469486E-4	0.00127500972194913	0.0014050107132066883	0.0020525156504318342	3.8750295471002964E-4
165-168	0.02269079801733488	6.031295988631913E-4	0.0013156350317171167	0.0014218858418795443	0.002103141036450403	3.9062797853833635E-4
>>END_MODULE
