##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293521
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8002007
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.33961629876105	39.0	39.0	39.0	37.0	39.0
2	40.06111829194851	41.0	40.0	41.0	38.0	41.0
3	40.1247998158462	41.0	40.0	41.0	38.0	41.0
4	40.15889776152408	41.0	40.0	41.0	38.0	41.0
5	40.17042486966082	41.0	40.0	41.0	38.0	41.0
6	40.16179141057987	41.0	40.0	41.0	38.0	41.0
7	40.1850495756877	41.0	40.0	41.0	38.0	41.0
8	40.16835963777587	41.0	40.0	41.0	38.0	41.0
9	40.144266057252885	41.0	40.0	41.0	38.0	41.0
10-14	40.0877305156069	41.0	40.0	41.0	38.0	41.0
15-19	39.952291868777415	41.0	40.0	41.0	38.0	41.0
20-24	39.758707909153294	41.0	40.0	41.0	37.4	41.0
25-29	39.496330133177835	40.0	39.0	41.0	36.4	41.0
30-34	39.279777460829514	40.0	38.4	41.0	35.4	41.0
35-39	38.90834511891829	40.0	37.6	41.0	35.0	41.0
40-44	38.36741497476821	39.4	36.2	41.0	35.0	41.0
45-49	37.86289864530236	38.8	35.0	41.0	34.8	41.0
50-54	37.173417693836065	36.8	35.0	39.8	34.0	41.0
55-59	36.47458363882961	35.8	35.0	38.8	34.0	40.8
60-64	35.58021176437361	34.8	34.6	36.8	33.4	39.4
65-69	35.339081657889075	35.0	34.8	36.0	33.4	38.8
70-74	35.23704925526808	35.0	35.0	35.8	34.0	37.0
75-79	35.0794775860606	35.0	35.0	35.0	33.2	36.8
80-84	35.00665450555091	35.0	35.0	35.0	33.0	36.0
85-89	34.87382210487944	35.0	34.8	35.0	32.6	36.0
90-94	39.573351043259564	40.6	39.8	40.6	37.8	40.6
95-99	39.88184905495107	41.0	40.0	41.0	37.8	41.0
100-104	39.76622246167366	41.0	40.0	41.0	37.0	41.0
105-109	39.60636951037215	41.0	39.4	41.0	36.4	41.0
110-114	39.40484637046799	40.4	39.0	41.0	35.4	41.0
115-119	39.10619316228594	40.0	38.0	41.0	35.0	41.0
120-124	38.79662784316258	40.0	37.8	41.0	35.0	41.0
125-129	38.07635816250006	39.4	36.2	40.4	34.4	40.6
130-134	37.97119911412042	39.0	35.2	40.8	34.0	41.0
135-139	37.43456919755668	37.8	35.0	40.4	34.0	41.0
140-144	36.71958636513237	36.4	35.0	39.4	33.6	41.0
145-149	36.12408410654903	35.2	35.0	38.0	33.6	40.0
150-154	35.64798214543808	35.0	35.0	36.6	34.0	39.0
155-159	35.17300895155879	35.0	35.0	35.8	34.0	37.0
160-164	34.860867486167635	35.0	35.0	35.0	33.2	36.2
165-169	34.67959939439313	35.0	35.0	35.0	33.0	36.0
170-174	34.58011664146609	35.0	35.0	35.0	33.0	35.8
175-178	34.34210261838892	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	7.0
30	169.0
31	1947.0
32	13245.0
33	56963.0
34	181854.0
35	506481.0
36	1394879.0
37	2692583.0
38	2639271.0
39	511295.0
40	3313.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.355878968863685	26.550364177387	27.801712745315022	23.292044108434297
2	23.570186829379182	27.382980294818537	27.210048679037648	21.836784196764636
3	20.54092179624437	26.55576282300178	29.715920018565345	23.18739536218851
4	22.008378648006683	25.57714583353901	29.54829957034529	22.866175948109017
5	22.88358408084372	26.547277451769286	27.421970513147514	23.14716795423948
6	22.665951179497842	26.81973409920786	27.53742904748771	22.97688567380658
7	22.997143091726862	26.838566874535353	26.979119113492402	23.185170920245383
8	23.073623904602933	27.25948627638041	26.694590494609667	22.97229932440699
9	23.01253922922087	27.331568192829625	26.95789943697875	22.69799314097076
10-14	23.031861881650443	27.09536994906403	26.874017980739083	22.99875018854645
15-19	23.008122837183222	27.04770690652982	26.984375294847908	22.959794961439048
20-24	22.93657828592252	26.98171596200803	27.041463472851245	23.0402422792182
25-29	22.979919912591928	26.92540258962533	26.899051700404662	23.195625797378085
30-34	22.95440131457021	27.00571994001005	26.968991654218748	23.070887091200994
35-39	22.850647343847612	27.066719636711138	26.991256068633778	23.091376950807465
40-44	22.991222077161392	27.010388768717647	26.852793305479487	23.145595848641474
45-49	23.07974986775193	26.903648047296137	26.908806753105814	23.107795331846123
50-54	23.07208429085353	27.01936651642519	26.855865034859377	23.052684157861897
55-59	23.0513594901879	26.9249826949664	26.84352812988042	23.180129684965284
60-64	23.02388638250379	26.971758460096325	26.912083431069227	23.092271726330658
65-69	22.983186593063465	26.989454020722555	26.927129656347464	23.10022972986652
70-74	22.985638477946846	26.983400539389677	26.878219426701328	23.152741555962148
75-79	22.944461308269286	26.966749716664832	26.960661244110383	23.128127730955498
80-84	22.930772242513658	27.0477169040217	26.895230159133828	23.12628069433081
85-89	22.96895266400042	26.96160350772	26.860906270139477	23.2085375581401
90-94	22.30395970811481	26.611554794966562	28.208534203444476	22.875951293474152
95-99	22.914816080358307	27.148082691462456	26.980194173921667	22.956907054257574
100-104	22.985666443872635	27.160338635229692	26.9387937213442	22.915201199553476
105-109	22.94106768051388	27.097560778848056	26.988547624966102	22.972823915671967
110-114	22.923047495641782	26.959100901599868	27.088117339964114	23.02973426279424
115-119	22.934251219563663	27.072656052609833	26.915296753870514	23.077795973955993
120-124	22.91390180807148	27.04574729994192	26.934572172854516	23.10577871913209
125-129	22.87197385840633	27.0599595959699	27.027681772543495	23.04038477308027
130-134	22.953712746594903	27.13256297040742	26.9002306434853	23.013493639512376
135-139	23.03276429288136	27.032473835032683	26.866883617059788	23.06787825502617
140-144	22.99124585479791	27.132916282344404	26.94406701208953	22.931770850768153
145-149	22.900000019401375	27.205417351708434	26.970325347477935	22.92425728141226
150-154	22.861820050556535	27.261538606984164	27.00570048812389	22.870940854335412
155-159	22.76518466354569	27.26542181150659	27.136390533538364	22.83300299140936
160-164	22.71612817690275	27.44199561759537	27.12315824581138	22.718717959690498
165-169	22.647866732029957	27.378787998588628	27.168731425081123	22.8046138443003
170-174	22.601069814279242	27.51511151503394	27.252099529900125	22.63171914078669
175-178	22.54830155898074	27.59124157138365	27.22117161547323	22.63928525416237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	3.0
12	7.5
13	21.0
14	39.0
15	73.5
16	147.5
17	293.0
18	558.0
19	1094.0
20	2083.0
21	3849.5
22	6646.5
23	10688.0
24	16410.5
25	23772.5
26	32774.5
27	43476.0
28	55972.5
29	70300.0
30	85798.0
31	102649.5
32	120549.5
33	139652.0
34	159825.5
35	179980.5
36	201820.5
37	224461.0
38	246159.5
39	267723.5
40	288022.0
41	305226.5
42	318655.5
43	328543.0
44	334850.0
45	335542.0
46	330364.5
47	319860.5
48	304819.5
49	287154.0
50	267156.0
51	247113.0
52	227793.0
53	209329.5
54	193405.5
55	179699.0
56	167978.5
57	158270.5
58	149446.0
59	140895.5
60	132394.0
61	122704.5
62	112099.5
63	101010.5
64	89150.0
65	77370.0
66	65315.5
67	53362.5
68	42876.5
69	33470.0
70	25195.5
71	18427.0
72	13106.0
73	9115.5
74	6114.5
75	3953.0
76	2481.0
77	1516.5
78	913.0
79	512.0
80	293.0
81	167.0
82	83.5
83	41.5
84	23.0
85	11.5
86	5.5
87	3.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	907.0
92-93	2016.0
94-95	3150.0
96-97	4298.0
98-99	5392.0
100-101	7439.0
102-103	7353.0
104-105	8921.0
106-107	8811.0
108-109	9940.0
110-111	10465.0
112-113	11543.0
114-115	11123.0
116-117	11752.0
118-119	11934.0
120-121	12449.0
122-123	12705.0
124-125	13505.0
126-127	13924.0
128-129	14547.0
130-131	15362.0
132-133	16745.0
134-135	83148.0
136-137	88825.0
138-139	85012.0
140-141	94672.0
142-143	89724.0
144-145	92139.0
146-147	79990.0
148-149	75029.0
150-151	74875.0
152-153	75720.0
154-155	68601.0
156-157	67460.0
158-159	75115.0
160-161	72238.0
162-163	73902.0
164-165	75193.0
166-167	80905.0
168-169	80275.0
170-171	83496.0
172-173	87707.0
174-175	93321.0
176-177	211452.0
178-179	5878927.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.37992510950636
#Duplication Level	Percentage of total
1	89.39623142329395
2	9.04117582615102
3	1.080590064285952
4	0.2840594739228623
5	0.08196051482032232
6	0.04535981147784367
7	0.021783917898585064
8	0.009394022539152115
9	0.015753860628482896
>10	0.023691084981841873
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.7490594547093E-5	1.2496864849031E-5	2.4993729698062E-5	2.4993729698062E-5	0.0	0.0
2	2.3744043213158898E-4	1.2496864849031E-5	4.9987459396124E-5	3.7490594547093E-5	0.0	0.0
3	2.3744043213158898E-4	2.4993729698062E-5	4.9987459396124E-5	4.9987459396124E-5	0.0	0.0
4	2.4993729698062E-4	2.4993729698062E-5	6.2484324245155E-5	4.9987459396124E-5	0.0	0.0
5	2.62434161829651E-4	2.4993729698062E-5	6.2484324245155E-5	4.9987459396124E-5	0.0	0.0
6	2.62434161829651E-4	2.4993729698062E-5	8.747805394321699E-5	6.2484324245155E-5	0.0	0.0
7	2.74931026678682E-4	2.4993729698062E-5	8.747805394321699E-5	7.4981189094186E-5	0.0	0.0
8	2.74931026678682E-4	2.4993729698062E-5	8.747805394321699E-5	8.747805394321699E-5	0.0	0.0
9	2.8742789152771297E-4	2.4993729698062E-5	9.9974918792248E-5	8.747805394321699E-5	0.0	0.0
10-14	2.924266374673254E-4	3.4991221577286796E-5	1.324667673997286E-4	9.9974918792248E-5	0.0	0.0
15-19	3.324166049842246E-4	6.74830701847674E-5	2.074479564939146E-4	1.299673944299224E-4	0.0	0.0
20-24	3.8490343735015476E-4	9.247679988282939E-5	2.674329077692634E-4	1.449636322487596E-4	0.0	0.0
25-29	4.198946589274416E-4	1.099724106714728E-4	2.9242663746732544E-4	1.549611241279844E-4	1.4996237818837201E-5	0.0
30-34	5.573601722667826E-4	1.5496112412798443E-4	3.2741785904461223E-4	2.3744043213158898E-4	2.9992475637674402E-5	0.0
35-39	0.0010222435446507357	1.6745798897701536E-4	3.5990970765209274E-4	2.74931026678682E-4	4.498871345651159E-5	3.24918486074806E-5
40-44	0.0013896513712122473	1.7495610788643398E-4	3.99899675168992E-4	3.024241293465502E-4	6.49836972149612E-5	7.74805620639922E-5
45-49	0.001687076754619185	1.87452972735465E-4	4.4988713456511605E-4	3.574103346822866E-4	8.747805394321699E-5	9.74755458224418E-5
50-54	0.0018770291003244563	2.12446702433527E-4	5.098720858404647E-4	4.0739779407841055E-4	1.024742917620542E-4	1.224692755205038E-4
55-59	0.0020369889703920527	2.12446702433527E-4	5.648582911762011E-4	4.37390269716085E-4	1.1996990255069761E-4	1.37465513339341E-4
60-64	0.0021894507215502314	2.149460754033332E-4	6.048482586931003E-4	4.548858805047284E-4	1.474630052185658E-4	1.549611241279844E-4
65-69	0.0022994231322217037	2.3494105916178277E-4	6.12346377602519E-4	4.873777291122089E-4	1.524617511581782E-4	1.7495610788643398E-4
70-74	0.004376402070130656	2.474379240108138E-4	6.12346377602519E-4	5.273676966291082E-4	1.7245673491662777E-4	1.7495610788643398E-4
75-79	0.04239186494088295	2.4993729698062E-4	6.523363451194183E-4	5.598595452365886E-4	1.87452972735465E-4	2.049485835241084E-4
80-84	0.05867527983917035	2.549360429202324E-4	6.823288207570926E-4	6.048482586931004E-4	2.899272644975192E-4	2.12446702433527E-4
85-89	0.058770256012022994	2.9242663746732544E-4	7.273175342136041E-4	6.2484324245155E-4	5.573601722667826E-4	2.12446702433527E-4
90-94	0.05882274284438891	3.224191131049998E-4	7.548106368814724E-4	6.673325829382553E-4	5.748557830554259E-4	2.12446702433527E-4
95-99	0.058995199579305546	3.4991221577286796E-4	7.74805620639922E-4	7.173200423343793E-4	5.798545289950383E-4	2.12446702433527E-4
100-104	0.05915016070343353	3.7490594547093E-4	7.948006043983714E-4	7.598093828210847E-4	5.873526479044569E-4	2.12446702433527E-4
105-109	0.05919764878985985	4.0239904813879813E-4	8.122962151870149E-4	8.297918259756584E-4	5.873526479044569E-4	2.12446702433527E-4
110-114	0.059320118065380345	4.37390269716085E-4	8.447880637944955E-4	9.172698799188754E-4	5.873526479044569E-4	2.12446702433527E-4
115-119	0.059432589849021625	4.62383999414147E-4	8.872774042812009E-4	9.472623555565496E-4	5.973501397836818E-4	2.12446702433527E-4
120-124	0.05967003028115322	5.098720858404648E-4	9.197692528886815E-4	9.822535771338366E-4	6.023488857232941E-4	2.12446702433527E-4
125-129	0.06017990236699368	5.24868323659302E-4	9.722560852546116E-4	0.0010097466798017047	6.12346377602519E-4	2.2494356728255802E-4
130-134	0.060709769436592585	5.348658155385268E-4	0.0010297416635601546	0.001062233512167635	6.148457505723251E-4	2.3744043213158898E-4
135-139	0.061272128354798984	5.473626803875578E-4	0.0010597341391978287	0.001087227241865697	6.2484324245155E-4	2.424391780712014E-4
140-144	0.061864479748643064	5.49862053357364E-4	0.0011022234796845341	0.0011022234796845341	6.37340107300581E-4	2.4993729698062E-4
145-149	0.06219939572659709	5.49862053357364E-4	0.0011422134472014332	0.0011122209715637588	6.773300748174802E-4	2.4993729698062E-4
150-154	0.06249682111000402	5.49862053357364E-4	0.0011497115661108519	0.0011447128201712395	6.873275666967049E-4	2.549360429202324E-4
155-159	0.06285173207171652	5.548607992969764E-4	0.0011847027876881388	0.0012071971444163943	7.048231774853484E-4	2.699322807390696E-4
160-164	0.0631741511848215	5.673576641460074E-4	0.0012071971444163946	0.0012271921281748442	7.298169071834103E-4	2.8742789152771297E-4
165-167	0.0637631700813725	5.99849512753488E-4	0.001224692755205038	0.0012455208632867563	7.581431341745472E-4	2.8742789152771297E-4
>>END_MODULE
