##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293522
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3241745
Total Bases	556 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.340307766341894	39.0	39.0	39.0	37.0	39.0
2	40.06163964161277	41.0	40.0	41.0	38.0	41.0
3	40.12428152121774	41.0	40.0	41.0	38.0	41.0
4	40.159350905145224	41.0	40.0	41.0	38.0	41.0
5	40.16324880581292	41.0	40.0	41.0	38.0	41.0
6	40.15548261815781	41.0	40.0	41.0	38.0	41.0
7	40.17352351896895	41.0	40.0	41.0	38.0	41.0
8	40.15413488722895	41.0	40.0	41.0	38.0	41.0
9	40.13722485883375	41.0	40.0	41.0	38.0	41.0
10-14	40.07587166788257	41.0	40.0	41.0	38.0	41.0
15-19	39.937285320097665	41.0	40.0	41.0	38.0	41.0
20-24	39.7338598193257	40.8	39.8	41.0	37.4	41.0
25-29	39.45393928270114	40.0	39.0	41.0	36.4	41.0
30-34	39.216605377659256	40.0	38.2	41.0	35.0	41.0
35-39	38.82979814883651	40.0	37.4	41.0	35.0	41.0
40-44	38.27812940252858	39.4	36.0	41.0	34.8	41.0
45-49	37.7765687307299	38.6	35.0	40.8	34.6	41.0
50-54	37.06747396849536	36.8	35.0	39.6	34.0	41.0
55-59	36.36255572230388	35.6	35.0	38.4	34.0	40.6
60-64	35.48545934365596	34.8	34.6	36.6	32.8	39.2
65-69	35.28091543289185	35.0	34.8	36.0	33.4	38.4
70-74	35.203300074496916	35.0	35.0	35.6	34.0	37.0
75-79	35.058117464513714	35.0	35.0	35.0	33.0	36.4
80-84	34.98553476599794	35.0	35.0	35.0	33.0	36.0
85-89	34.85949339013401	35.0	34.8	35.0	32.6	36.2
90-94	39.62287351328732	40.6	39.8	40.6	37.8	40.6
95-99	39.916354379896234	41.0	40.0	41.0	38.0	41.0
100-104	39.8001549574555	41.0	40.0	41.0	37.0	41.0
105-109	39.635848317493085	41.0	39.6	41.0	36.4	41.0
110-114	39.42974022242218	40.4	39.0	41.0	35.4	41.0
115-119	39.119228948010054	40.0	38.0	41.0	35.0	41.0
120-124	38.802110305383174	40.0	37.8	41.0	35.0	41.0
125-129	38.08435099386281	39.4	36.2	40.4	34.4	40.6
130-134	37.99703992234484	39.0	35.2	40.8	34.2	41.0
135-139	37.45491138450503	37.8	35.0	40.4	34.0	41.0
140-144	36.742675262432826	36.4	35.0	39.4	33.8	41.0
145-149	36.14727478778576	35.2	35.0	38.0	33.6	40.4
150-154	35.67164375470926	35.0	35.0	36.6	34.0	39.0
155-159	35.194550422821195	35.0	35.0	35.8	34.0	37.0
160-164	34.86679345625343	35.0	35.0	35.0	33.2	36.2
165-169	34.6781661443947	35.0	35.0	35.0	33.0	36.0
170-174	34.57635239357462	35.0	35.0	35.0	33.0	35.8
175-178	34.336472696785805	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	41.0
31	819.0
32	5075.0
33	21631.0
34	71553.0
35	204436.0
36	581687.0
37	1115826.0
38	1053423.0
39	186496.0
40	756.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.425853976793363	26.482928176028654	27.74656859191577	23.344649255262212
2	23.675859760715294	27.253747595816453	27.173883201794098	21.896509441674162
3	20.569045375253143	26.480028503167276	29.648908226896314	23.302017894683264
4	22.099023828215977	25.43158083069458	29.47560650205368	22.993788839035766
5	22.97487927026956	26.44634294184151	27.337622175710923	23.24115561217801
6	22.781650006400874	26.741831945449135	27.385405082756353	23.091112965393638
7	23.109282192152683	26.78184126141939	26.854857491875517	23.254019054552412
8	23.204570377990866	27.180392041940376	26.56674106075586	23.0482965193129
9	23.123873099210456	27.22404137277917	26.876790123837623	22.775295404172752
10-14	23.121473157203916	26.98915553197429	26.79110170602561	23.098269604796183
15-19	23.081112178780256	26.962435355032554	26.917619985532482	23.03883248065471
20-24	23.029047627126747	26.902128329032664	26.93215536693972	23.136668676900868
25-29	23.0680944984877	26.855752071800836	26.798887636134243	23.277265793577225
30-34	23.032416183259325	26.904571457656296	26.88595802569295	23.177054333391432
35-39	22.945660439053658	26.97885243904132	26.907674724569635	23.167812397335386
40-44	23.085066839001833	26.92328977140398	26.75189442722978	23.239748962364406
45-49	23.185648470191207	26.801404798958583	26.80126290007388	23.211683830776327
50-54	23.172217432277986	26.930144104486935	26.754189487451974	23.143448975783105
55-59	23.174882663503762	26.814496513451857	26.7554850859645	23.255135737079875
60-64	23.142714803292673	26.88683409706809	26.787029824986234	23.183421274653004
65-69	23.070525288077874	26.89205967773529	26.832061127571727	23.20535390661511
70-74	23.083684867255137	26.88924637810809	26.781366208631464	23.245702546005315
75-79	23.057248488082806	26.87187918852347	26.84276523909191	23.22810708430182
80-84	23.046778818198224	26.93221089259026	26.801269069590607	23.219741219620914
85-89	23.074146794396228	26.86211284354568	26.74954384135705	23.314196520701042
90-94	22.40226766428	26.544822534525043	28.106254372147983	22.946655429046974
95-99	23.01219797641185	27.041445698902482	26.893366674839413	23.05298964984625
100-104	23.088806236950134	27.040257939047557	26.870923265570802	23.000012558431504
105-109	23.062267856545958	26.982758508420186	26.894653065671886	23.060320569361966
110-114	23.03291958851152	26.86450313765596	26.96206367025286	23.140513603579656
115-119	23.05027221064605	26.982752335998267	26.794872563074325	23.17210289028136
120-124	22.995508582056942	26.957798086973572	26.846450764956852	23.20024256601263
125-129	22.973353545841448	26.97599251988745	26.91851029059601	23.132143643675096
130-134	23.029781598493333	27.003343052383332	26.837514408101388	23.129360941021943
135-139	23.1118528517803	26.925613014653887	26.777795246981373	23.18473888658444
140-144	23.062634290324006	27.04018768168302	26.86170937543242	23.035468652560557
145-149	22.988371095947297	27.10606466883256	26.883438593800346	23.022125641419798
150-154	22.958034573456267	27.152485800854603	26.910817566783496	22.978662058905634
155-159	22.86492033593212	27.165990826212177	27.06553402912179	22.903554808733915
160-164	22.840265804391738	27.303767689184642	27.029631364530076	22.826335141893537
165-169	22.75436657875178	27.275572780244957	27.088412084157504	22.88164855684576
170-174	22.682809326789346	27.427007324908825	27.14395371480641	22.746229633495414
175-178	22.634209977262362	27.524185403508216	27.104401925327444	22.737202693901978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	4.5
13	7.0
14	13.0
15	29.0
16	54.0
17	99.0
18	193.5
19	386.5
20	749.0
21	1413.5
22	2464.5
23	4011.5
24	6212.0
25	9084.0
26	12627.0
27	16872.0
28	21849.5
29	27517.5
30	33492.0
31	40167.5
32	47517.5
33	54811.5
34	62682.0
35	71120.0
36	80083.5
37	89179.5
38	98450.5
39	107463.5
40	115210.5
41	122235.0
42	128239.0
43	133064.0
44	135676.5
45	135587.5
46	133248.5
47	129048.5
48	123944.0
49	117006.5
50	109009.0
51	101132.5
52	93393.5
53	86544.0
54	79908.0
55	74121.5
56	69686.5
57	65709.0
58	62030.0
59	58407.5
60	54942.5
61	51380.5
62	47071.5
63	42306.5
64	37251.5
65	32281.5
66	27274.0
67	22364.0
68	17789.0
69	13650.0
70	10405.0
71	7640.0
72	5427.5
73	3793.0
74	2558.0
75	1654.5
76	1013.5
77	614.5
78	379.0
79	216.5
80	112.0
81	68.0
82	41.0
83	18.5
84	6.0
85	3.5
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	392.0
92-93	785.0
94-95	1271.0
96-97	1699.0
98-99	2087.0
100-101	2518.0
102-103	2950.0
104-105	3217.0
106-107	3539.0
108-109	3849.0
110-111	4158.0
112-113	4323.0
114-115	4397.0
116-117	4510.0
118-119	4663.0
120-121	4785.0
122-123	5035.0
124-125	5370.0
126-127	5520.0
128-129	5791.0
130-131	6031.0
132-133	6677.0
134-135	31496.0
136-137	34601.0
138-139	33783.0
140-141	34402.0
142-143	35138.0
144-145	36849.0
146-147	31969.0
148-149	30771.0
150-151	29753.0
152-153	29361.0
154-155	26330.0
156-157	26972.0
158-159	27315.0
160-161	28364.0
162-163	30168.0
164-165	30568.0
166-167	30937.0
168-169	32820.0
170-171	33907.0
172-173	35664.0
174-175	37819.0
176-177	81760.0
178-179	2407431.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.39452729788883
#Duplication Level	Percentage of total
1	94.95494939205852
2	4.62040664315003
3	0.32465320011427196
4	0.0523323318120413
5	0.021256188875712907
6	0.014028733674602764
7	0.005478091046598359
8	0.0022245275745066025
9	0.0011290698563739977
>10	0.0035418218373513776
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	1.8508550178993104E-4	0.0	0.0	3.0847583631655175E-5	0.0	0.0
3	1.8508550178993104E-4	0.0	3.0847583631655175E-5	6.169516726331035E-5	3.0847583631655175E-5	0.0
4	1.8508550178993104E-4	0.0	6.169516726331035E-5	6.169516726331035E-5	3.0847583631655175E-5	0.0
5	1.8508550178993104E-4	0.0	6.169516726331035E-5	6.169516726331035E-5	3.0847583631655175E-5	0.0
6	1.8508550178993104E-4	3.0847583631655175E-5	6.169516726331035E-5	6.169516726331035E-5	3.0847583631655175E-5	0.0
7	2.1593308542158622E-4	3.0847583631655175E-5	6.169516726331035E-5	6.169516726331035E-5	3.0847583631655175E-5	0.0
8	2.467806690532414E-4	6.169516726331035E-5	9.254275089496552E-5	6.169516726331035E-5	3.0847583631655175E-5	0.0
9	2.776282526848966E-4	6.169516726331035E-5	9.254275089496552E-5	6.169516726331035E-5	3.0847583631655175E-5	0.0
10-14	2.837977694112276E-4	7.403420071597241E-5	1.2955985125295174E-4	6.169516726331035E-5	3.0847583631655175E-5	0.0
15-19	3.269843864955449E-4	1.1105130107395863E-4	1.5423791815827586E-4	9.871226762129656E-5	3.7017100357986205E-5	0.0
20-24	3.393234199482069E-4	1.233903345266207E-4	1.5423791815827586E-4	2.0359405196892418E-4	6.169516726331035E-5	0.0
25-29	3.393234199482069E-4	1.3572936797928275E-4	1.604074348846069E-4	2.5295018577957244E-4	6.786468398964139E-5	0.0
30-34	4.6271375447482757E-4	1.7274646833726894E-4	1.8508550178993104E-4	2.9613680286388964E-4	9.254275089496552E-5	0.0
35-39	9.747836427603035E-4	2.3444163560057936E-4	1.8508550178993104E-4	3.084758363165517E-4	9.254275089496552E-5	0.0
40-44	0.0013634631965191587	3.393234199482069E-4	1.8508550178993104E-4	3.208148697692138E-4	1.418988847056138E-4	0.0
45-49	0.0017027866164673656	4.010185872115173E-4	2.3444163560057936E-4	3.393234199482069E-4	1.974245352425931E-4	0.0
50-54	0.001838515984446648	4.380356875695035E-4	3.0847583631655177E-4	3.64001486853531E-4	2.7145873595856555E-4	6.169516726331035E-6
55-59	0.0020050929360575865	4.6271375447482757E-4	3.393234199482069E-4	4.010185872115173E-4	2.8996728613755866E-4	3.7017100357986205E-5
60-64	0.0022148565047528414	4.935613381064828E-4	3.4549293667453793E-4	4.3186617084317244E-4	3.393234199482069E-4	7.403420071597241E-5
65-69	0.0023012297389214758	5.18239405011807E-4	3.948490704851862E-4	4.442052042958345E-4	3.393234199482069E-4	9.254275089496552E-5
70-74	0.004368017842242372	5.30578438464469E-4	4.010185872115173E-4	4.8122230465382073E-4	3.393234199482069E-4	9.254275089496552E-5
75-79	0.04192186615541938	6.046126391804414E-4	4.503747210221656E-4	5.24408921738138E-4	3.393234199482069E-4	9.254275089496552E-5
80-84	0.05818471224602799	6.354602228120966E-4	5.059003715591449E-4	6.231211893594345E-4	4.010185872115173E-4	9.254275089496552E-5
85-89	0.05836979774781792	6.909858733490759E-4	5.737650555487862E-4	6.539687729910898E-4	6.046126391804414E-4	9.254275089496552E-5
90-94	0.058462340498712885	7.09494423528069E-4	6.169516726331034E-4	6.786468398964138E-4	6.169516726331034E-4	9.254275089496552E-5
95-99	0.05852403566597619	7.09494423528069E-4	6.169516726331034E-4	7.526810406123862E-4	6.169516726331034E-4	9.254275089496552E-5
100-104	0.058567222283060515	7.09494423528069E-4	6.477992562647587E-4	8.267152413283588E-4	6.169516726331034E-4	9.254275089496552E-5
105-109	0.05864125648377648	7.34172490433393E-4	6.601382897174207E-4	8.637323416863449E-4	6.416297395384276E-4	9.254275089496552E-5
110-114	0.058727629717945116	7.403420071597242E-4	6.97155390075407E-4	8.76071375139007E-4	6.477992562647587E-4	9.254275089496552E-5
115-119	0.05893122376991404	7.465115238860551E-4	7.09494423528069E-4	9.254275089496551E-4	6.601382897174207E-4	9.254275089496552E-5
120-124	0.05911630927170397	7.711895907913793E-4	7.403420071597242E-4	0.0010118007431182898	6.786468398964138E-4	9.254275089496552E-5
125-129	0.05946797172510484	7.711895907913793E-4	8.143762078756965E-4	0.0010734959103816	6.786468398964138E-4	9.254275089496552E-5
130-134	0.05988749886249535	8.020371744230346E-4	8.328847580546895E-4	0.0011413605943712414	7.09494423528069E-4	9.871226762129656E-5
135-139	0.060485941984949464	8.328847580546895E-4	8.575628249600138E-4	0.0012153947950872138	7.09494423528069E-4	1.233903345266207E-4
140-144	0.060954825256150626	8.452237915073518E-4	9.932921929392965E-4	0.0012400728619925377	7.156639402544001E-4	1.233903345266207E-4
145-149	0.06125713157574084	8.637323416863449E-4	0.0010426483267499446	0.0012832594790768551	7.403420071597242E-4	1.233903345266207E-4
150-154	0.061281809642646165	8.637323416863449E-4	0.0010488178434762759	0.0013387851296138345	7.403420071597242E-4	1.233903345266207E-4
155-159	0.061540929345152075	9.130884754969933E-4	0.001054987360202607	0.0013696327132454898	7.403420071597242E-4	1.233903345266207E-4
160-164	0.061991304066174244	9.315970256759863E-4	0.001079665427107931	0.001437497397235131	7.711895907913793E-4	1.233903345266207E-4
165-167	0.06257946466075114	9.562750925813105E-4	0.001079665427107931	0.001449836430687793	7.711895907913793E-4	1.233903345266207E-4
>>END_MODULE
