##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293523
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3965184
Total Bases	679.3 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.32228970963264	39.0	39.0	39.0	37.0	39.0
2	40.02445737700949	41.0	40.0	41.0	38.0	41.0
3	40.088268539366645	41.0	40.0	41.0	38.0	41.0
4	40.123693881544966	41.0	40.0	41.0	38.0	41.0
5	40.141807542852995	41.0	40.0	41.0	38.0	41.0
6	40.13033367430112	41.0	40.0	41.0	38.0	41.0
7	40.15473985570405	41.0	40.0	41.0	38.0	41.0
8	40.12562443508296	41.0	40.0	41.0	38.0	41.0
9	40.106470468961845	41.0	40.0	41.0	38.0	41.0
10-14	40.04797169563884	41.0	40.0	41.0	38.0	41.0
15-19	39.90325230808961	41.0	40.0	41.0	38.0	41.0
20-24	39.70043332163148	40.6	39.8	41.0	37.2	41.0
25-29	39.420752025631096	40.0	38.8	41.0	36.4	41.0
30-34	39.192947767367166	40.0	38.0	41.0	35.0	41.0
35-39	38.803550755778296	40.0	37.4	41.0	35.0	41.0
40-44	38.25284501299309	39.4	36.0	41.0	34.8	41.0
45-49	37.751653845067466	38.4	35.0	40.8	34.4	41.0
50-54	37.06065085504229	36.8	35.0	39.6	34.0	41.0
55-59	36.35877341379366	35.6	35.0	38.6	34.0	40.6
60-64	35.48991542384918	34.8	34.6	36.6	32.8	39.4
65-69	35.281102112789725	35.0	34.8	36.0	33.4	38.6
70-74	35.20606983181612	35.0	35.0	35.6	33.8	37.0
75-79	35.05759419991607	35.0	35.0	35.0	33.0	36.6
80-84	34.989491130802506	35.0	35.0	35.0	33.0	36.0
85-89	34.85744858246175	35.0	34.8	35.0	32.6	36.2
90-94	39.588176567442765	40.6	39.8	40.6	37.8	40.6
95-99	39.87922842058792	41.0	40.0	41.0	37.8	41.0
100-104	39.76170484325313	41.0	40.0	41.0	37.0	41.0
105-109	39.586222322629084	41.0	39.2	41.0	36.2	41.0
110-114	39.383237108794994	40.4	39.0	41.0	35.4	41.0
115-119	39.08911030022315	40.0	38.0	41.0	35.0	41.0
120-124	38.775656076409234	40.0	37.4	41.0	35.0	41.0
125-129	38.05482493579266	39.4	36.0	40.4	34.4	40.6
130-134	37.961306953540365	39.0	35.2	40.8	34.0	41.0
135-139	37.41460137980458	37.6	35.0	40.4	34.0	41.0
140-144	36.69436164158729	36.4	35.0	39.2	33.8	41.0
145-149	36.095520369419	35.2	35.0	37.8	33.6	40.0
150-154	35.61597951592953	35.0	35.0	36.6	34.0	38.8
155-159	35.15508441772017	35.0	35.0	35.8	34.0	37.0
160-164	34.838967388989644	35.0	35.0	35.0	33.0	36.0
165-169	34.662227557103016	35.0	35.0	35.0	33.0	36.0
170-174	34.55815352726705	35.0	35.0	35.0	33.0	35.6
175-178	34.32041682897747	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	71.0
31	957.0
32	6386.0
33	27581.0
34	91091.0
35	259542.0
36	724668.0
37	1372262.0
38	1267303.0
39	214566.0
40	757.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.43290601394538	26.53316970914843	27.724413293304927	23.309510983601264
2	23.62127457389115	27.293084003163536	27.185522790367354	21.900118632577957
3	20.58915803150623	26.50116615017109	29.648535856091417	23.261139962231262
4	22.07370452417845	25.46867938537026	29.49905981664407	22.95855627380722
5	22.95237749370521	26.431005471625024	27.426974385047455	23.189642649622314
6	22.729437019820516	26.77454564529666	27.462685212085997	23.033332122796825
7	23.042058073471498	26.808465887081155	26.935748757182516	23.213727282264834
8	23.14427275001614	27.2107423009878	26.62670887403964	23.01827607495642
9	23.081652705145586	27.21729937374911	26.92379470914843	22.77725321195687
10-14	23.08828039253664	27.032112507263218	26.797833341403575	23.081773758796565
15-19	23.053174833752987	26.990328822067273	26.937125742462392	23.01937060171735
20-24	22.97711783362386	26.909747441732847	26.98377679320808	23.129357931435212
25-29	23.0288884450255	26.8786013461166	26.822654383756213	23.269855825101686
30-34	22.96797323907289	26.94514050293757	26.926210738265866	23.160675519723675
35-39	22.89801431661179	27.002156772548258	26.93709547904965	23.162733431790304
40-44	23.058491106591774	26.9564539754019	26.772588611272518	23.212466306733813
45-49	23.139254067402675	26.836787397507912	26.837301875524567	23.186656659564854
50-54	23.136843082187358	26.971510023242303	26.771680708890177	23.11996618568016
55-59	23.14752102298405	26.841034363096387	26.767226943314608	23.244217670604943
60-64	23.09236595325715	26.917409129059333	26.82918623700691	23.16103868067661
65-69	23.027370230486152	26.9463409516431	26.854188859836015	23.172099958034735
70-74	23.060675116211506	26.89403568661631	26.827925261475887	23.2173639356963
75-79	23.033135410613983	26.898620593647106	26.876170185292786	23.192073810446125
80-84	23.019486611466203	26.96760099909613	26.828878558977337	23.184033830460326
85-89	23.024954201368715	26.878001121763834	26.801137097294852	23.2959075795726
90-94	22.398587294732977	26.565941716070657	28.10844596367041	22.927025025525953
95-99	22.978691754592003	27.07831776547524	26.92465134466071	23.018339135272043
100-104	23.060122104292365	27.090426719568345	26.870056465167032	22.979394710972258
105-109	23.01424867615226	27.038398912825514	26.92370618334547	23.023646227676757
110-114	22.999421252866707	26.885752771944375	27.010314418689845	23.104511556499077
115-119	23.015102477816963	27.00164560085162	26.844882563289357	23.13836935804206
120-124	22.984603397146234	26.974725170510904	26.863332402069968	23.177339030272893
125-129	22.933513806144294	26.993251061689318	26.974309654171293	23.098925477995095
130-134	23.017614062482618	27.056490365648244	26.84089550331381	23.085000068555328
135-139	23.097203468339497	26.97302034740333	26.809834328733075	23.119941855524097
140-144	23.05635113323477	27.072540588929428	26.880979830998143	22.99012844683766
145-149	22.963671077877283	27.12258175294437	26.91869719798332	22.995049971195026
150-154	22.935164357248162	27.20523755118395	26.936621619355233	22.922976472212657
155-159	22.832223069450166	27.204889126945154	27.084947408583904	22.877940395020783
160-164	22.79306641069551	27.360973922051677	27.054477156684055	22.791482510568756
165-169	22.720086029512046	27.356286067539436	27.086529243762563	22.837098659185955
170-174	22.68265787176952	27.426358172546212	27.19009193494657	22.700892020737697
175-178	22.610528727903212	27.565764569093353	27.1461780877549	22.677528615248534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	3.0
12	7.0
13	10.0
14	18.5
15	35.5
16	58.0
17	116.0
18	242.0
19	510.0
20	990.5
21	1773.0
22	3063.0
23	4996.0
24	7813.5
25	11489.5
26	15728.0
27	20845.5
28	26995.5
29	33887.5
30	41462.0
31	49505.5
32	57996.0
33	67481.5
34	77775.0
35	88070.0
36	99052.5
37	110080.0
38	120606.0
39	131445.0
40	141814.5
41	150638.5
42	157750.0
43	162713.0
44	165723.0
45	166210.5
46	163354.5
47	157845.5
48	150675.0
49	142457.0
50	133264.0
51	123510.5
52	113539.0
53	104560.5
54	97081.5
55	90489.5
56	84649.5
57	79578.0
58	75307.0
59	71240.0
60	66498.0
61	61864.5
62	56914.5
63	51122.0
64	45234.0
65	39119.5
66	32865.5
67	27109.5
68	21763.5
69	16895.0
70	12775.5
71	9350.0
72	6720.0
73	4646.0
74	3086.5
75	1995.0
76	1248.0
77	787.0
78	484.0
79	274.0
80	146.0
81	72.5
82	39.5
83	22.5
84	10.0
85	4.5
86	1.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	429.0
92-93	1057.0
94-95	1659.0
96-97	2127.0
98-99	2725.0
100-101	3261.0
102-103	3753.0
104-105	4291.0
106-107	4491.0
108-109	5098.0
110-111	5384.0
112-113	5454.0
114-115	5673.0
116-117	5757.0
118-119	6222.0
120-121	6205.0
122-123	6504.0
124-125	6782.0
126-127	7285.0
128-129	7390.0
130-131	8012.0
132-133	8706.0
134-135	41388.0
136-137	43182.0
138-139	42621.0
140-141	43211.0
142-143	43739.0
144-145	45355.0
146-147	39910.0
148-149	37823.0
150-151	37458.0
152-153	36684.0
154-155	33255.0
156-157	33193.0
158-159	34501.0
160-161	35991.0
162-163	37129.0
164-165	37064.0
166-167	38985.0
168-169	40808.0
170-171	42085.0
172-173	44899.0
174-175	47149.0
176-177	100412.0
178-179	2920077.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.9350129893828
#Duplication Level	Percentage of total
1	94.07666240192421
2	5.382501465307922
3	0.4253033869793119
4	0.0726476682720034
5	0.0252222658173262
6	0.004256556420123265
7	0.0021549156862188086
8	0.003272651596131055
9	0.0011044170666563828
>10	0.006874270930124381
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.5219510620440313E-5	0.0	0.0
2	1.2609755310220155E-4	2.5219510620440313E-5	0.0	2.5219510620440313E-5	0.0	0.0
3	1.2609755310220155E-4	5.0439021240880626E-5	0.0	2.5219510620440313E-5	0.0	0.0
4	1.5131706372264187E-4	5.0439021240880626E-5	0.0	2.5219510620440313E-5	0.0	0.0
5	1.5131706372264187E-4	7.565853186132094E-5	0.0	2.5219510620440313E-5	0.0	0.0
6	1.5131706372264187E-4	1.0087804248176125E-4	0.0	2.5219510620440313E-5	0.0	0.0
7	1.7653657434308219E-4	1.0087804248176125E-4	0.0	5.0439021240880626E-5	0.0	0.0
8	1.7653657434308219E-4	1.2609755310220155E-4	0.0	5.0439021240880626E-5	0.0	0.0
9	1.7653657434308219E-4	1.5131706372264187E-4	0.0	5.0439021240880626E-5	2.5219510620440313E-5	0.0
10-14	2.4210730195622698E-4	1.5131706372264187E-4	0.0	7.565853186132095E-5	3.5307314868616436E-5	0.0
15-19	3.0767802956937183E-4	1.7653657434308219E-4	2.521951062044031E-5	1.1096584672993736E-4	5.043902124088062E-5	0.0
20-24	3.328975401898121E-4	1.7653657434308219E-4	6.557072761314481E-5	1.2609755310220155E-4	7.565853186132095E-5	0.0
25-29	3.7324875718251657E-4	1.7653657434308219E-4	1.0087804248176124E-4	1.2609755310220155E-4	1.0087804248176124E-4	0.0
30-34	5.346536251533346E-4	1.7653657434308219E-4	1.4627316159855378E-4	1.61404867970818E-4	1.0592194460584933E-4	0.0
35-39	9.482535993285558E-4	1.9671218283943444E-4	2.2193169345987476E-4	2.269755955839628E-4	1.6644877009490606E-4	0.0
40-44	0.001447599909613274	2.0175608496352248E-4	3.1272193169345987E-4	2.723707147007554E-4	2.269755955839628E-4	5.043902124088062E-5
45-49	0.001805716960423526	2.0679998708761057E-4	3.6820485505842854E-4	2.7741461682484345E-4	2.269755955839628E-4	1.0087804248176124E-4
50-54	0.00208313157724837	2.521951062044031E-4	4.0351216992704495E-4	3.0263412744528374E-4	2.521951062044031E-4	1.0087804248176124E-4
55-59	0.002340370585576861	2.572390083284912E-4	4.0351216992704495E-4	3.328975401898121E-4	3.177658338175479E-4	1.0087804248176124E-4
60-64	0.0024462925301827103	2.7741461682484345E-4	4.489072890438375E-4	3.8838046355478084E-4	3.429853444379882E-4	1.0592194460584933E-4
65-69	0.0025572583769126475	2.875024210730195E-4	4.943024081606301E-4	4.993463102847182E-4	3.5307314868616437E-4	1.2609755310220155E-4
70-74	0.004272185099102589	3.0263412744528374E-4	5.49785331525599E-4	5.396975272774228E-4	3.6820485505842854E-4	1.3114145522628962E-4
75-79	0.04107249499644909	3.0263412744528374E-4	5.548292336496869E-4	5.548292336496869E-4	3.7829265930660466E-4	1.5131706372264187E-4
80-84	0.05767702078894699	3.2280973594163594E-4	5.548292336496869E-4	5.69960940021951E-4	4.8421460391245396E-4	1.5131706372264187E-4
85-89	0.05777789883142875	3.581170508102524E-4	5.548292336496869E-4	5.800487442701272E-4	7.414536122409452E-4	1.5131706372264187E-4
90-94	0.05779303053780103	3.7829265930660466E-4	5.69960940021951E-4	5.800487442701272E-4	7.565853186132093E-4	1.5131706372264187E-4
95-99	0.0578838207760346	3.7829265930660466E-4	5.850926463942152E-4	6.052682548905675E-4	7.565853186132093E-4	1.5131706372264187E-4
100-104	0.05803513783975724	3.7829265930660466E-4	6.103121570146555E-4	6.153560591387437E-4	7.818048292336496E-4	1.5131706372264187E-4
105-109	0.058156191490735365	4.0855607205113304E-4	6.304877655110078E-4	6.557072761314482E-4	7.818048292336496E-4	1.5131706372264187E-4
110-114	0.058226806120472596	4.287316805474853E-4	6.304877655110078E-4	6.557072761314482E-4	7.818048292336496E-4	1.5131706372264187E-4
115-119	0.058272201239589386	4.287316805474853E-4	6.557072761314482E-4	6.758828846278005E-4	7.818048292336496E-4	1.5131706372264187E-4
120-124	0.058529440247917876	4.337755826715734E-4	6.657950803796243E-4	7.061462973723286E-4	7.818048292336496E-4	1.5131706372264187E-4
125-129	0.059028786558202595	4.943024081606301E-4	6.809267867518885E-4	7.464975143650331E-4	7.818048292336496E-4	1.5131706372264187E-4
130-134	0.05962901091096908	5.59873135773775E-4	7.011023952482405E-4	7.969365356059139E-4	7.818048292336496E-4	1.5131706372264187E-4
135-139	0.06027463038285236	5.901365485183033E-4	7.061462973723287E-4	8.070243398540899E-4	8.221560462263542E-4	1.5131706372264187E-4
140-144	0.060950513267480154	6.304877655110078E-4	7.364097101168571E-4	8.27199948350442E-4	8.625072632190587E-4	1.5131706372264187E-4
145-149	0.061253147394925433	6.304877655110078E-4	7.969365356059139E-4	8.87726773839499E-4	8.826828717154111E-4	1.5131706372264187E-4
150-154	0.06141959616502034	6.708389825037124E-4	8.070243398540899E-4	9.432096972044679E-4	8.826828717154111E-4	1.5131706372264187E-4
155-159	0.06170205468396926	6.809267867518885E-4	8.171121441022661E-4	0.0010087804248176124	8.826828717154111E-4	1.5131706372264187E-4
160-164	0.06211565465814449	6.910145910000645E-4	8.423316547227064E-4	0.0010541755439344052	8.826828717154111E-4	1.5131706372264187E-4
165-167	0.06281339445197666	7.061462973723289E-4	8.574633610949706E-4	0.0010592194460584932	8.82682871715411E-4	1.5131706372264187E-4
>>END_MODULE
