##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293524
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8004124
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.34019338031245	39.0	39.0	39.0	37.0	39.0
2	40.057498109724435	41.0	40.0	41.0	38.0	41.0
3	40.112656300677	41.0	40.0	41.0	38.0	41.0
4	40.14807029476305	41.0	40.0	41.0	38.0	41.0
5	40.15117107131274	41.0	40.0	41.0	38.0	41.0
6	40.147582171390646	41.0	40.0	41.0	38.0	41.0
7	40.172724710411785	41.0	40.0	41.0	38.0	41.0
8	40.15575558299697	41.0	40.0	41.0	38.0	41.0
9	40.13419069969431	41.0	40.0	41.0	38.0	41.0
10-14	40.07873433744904	41.0	40.0	41.0	38.0	41.0
15-19	39.944303211694375	41.0	40.0	41.0	38.0	41.0
20-24	39.74601328015409	40.8	39.8	41.0	37.4	41.0
25-29	39.478884659957785	40.0	39.0	41.0	36.4	41.0
30-34	39.254970512700694	40.0	38.4	41.0	35.0	41.0
35-39	38.882198601620864	40.0	37.6	41.0	35.0	41.0
40-44	38.33318749184795	39.4	36.2	41.0	35.0	41.0
45-49	37.83430179242601	38.6	35.0	40.8	34.8	41.0
50-54	37.14195529704437	36.8	35.0	39.8	34.0	41.0
55-59	36.44078407580893	35.6	35.0	38.8	34.0	40.8
60-64	35.56509321944537	34.8	34.6	36.8	33.2	39.4
65-69	35.31961266467136	35.0	34.8	36.0	33.4	38.8
70-74	35.219085536405984	35.0	35.0	35.8	33.8	37.0
75-79	35.06167800498842	35.0	35.0	35.0	33.0	36.6
80-84	34.98945058822177	35.0	35.0	35.0	33.0	36.0
85-89	34.858886793857764	35.0	34.8	35.0	32.6	36.2
90-94	39.575579234960585	40.6	39.8	40.6	37.8	40.6
95-99	39.87893257265155	41.0	40.0	41.0	37.8	41.0
100-104	39.76388719171642	41.0	40.0	41.0	37.0	41.0
105-109	39.598312312146696	41.0	39.4	41.0	36.2	41.0
110-114	39.398212229386495	40.4	39.0	41.0	35.4	41.0
115-119	39.10089726295751	40.0	38.0	41.0	35.0	41.0
120-124	38.789489513506716	40.0	37.8	41.0	35.0	41.0
125-129	38.08517037530182	39.4	36.2	40.4	34.4	40.6
130-134	37.95831508957359	39.0	35.2	40.8	34.0	41.0
135-139	37.40635964881852	37.6	35.0	40.4	34.0	41.0
140-144	36.69663923036745	36.4	35.0	39.4	33.6	41.0
145-149	36.102995734745264	35.2	35.0	37.8	33.6	40.0
150-154	35.627780753960465	35.0	35.0	36.6	34.0	39.0
155-159	35.15740226172551	35.0	35.0	35.8	34.0	37.0
160-164	34.845367215448796	35.0	35.0	35.0	33.0	36.0
165-169	34.66572785083797	35.0	35.0	35.0	33.0	36.0
170-174	34.56810218864787	35.0	35.0	35.0	33.0	35.6
175-178	34.333417391081085	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	8.0
30	183.0
31	2104.0
32	13485.0
33	58326.0
34	186224.0
35	514327.0
36	1416789.0
37	2709294.0
38	2612719.0
39	488424.0
40	2241.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.329639071058867	26.565355559209223	27.80156829154571	23.303437078186196
2	23.612965016533977	27.365942856457497	27.194843558145777	21.826248568862752
3	20.574706238933828	26.552487192852087	29.660197668102096	23.21260890011199
4	22.023009638531338	25.548942020388488	29.555051870760625	22.87299647031955
5	22.907041420147912	26.496078771393343	27.40589725996249	23.19098254849625
6	22.68388145910783	26.81580145435028	27.500398544550286	22.999918541991605
7	23.030202930389386	26.86074078812372	26.942623577545778	23.166432703941116
8	23.096831083576415	27.27425262277296	26.665441464924832	22.96347482872579
9	23.048468514480785	27.321890565413533	26.911764485407772	22.717876434697914
10-14	23.057046592481576	27.067081919270613	26.84617079895314	23.029700689294668
15-19	23.011969829552868	27.035125892602363	26.978660000769604	22.97424427707517
20-24	22.95621357190368	26.98139359160353	27.011852889835293	23.050539946657498
25-29	22.98350950085231	26.929555314235508	26.87090554818991	23.21602963672227
30-34	22.95654839930016	26.98521662083196	26.956898718710505	23.101336261157375
35-39	22.866969577182964	27.05148246079146	26.977832927126066	23.10371503489951
40-44	23.007649556653543	27.001595677428288	26.835276415008064	23.155478350910105
45-49	23.109522041387663	26.89116010696486	26.878569097630173	23.120748754017303
50-54	23.09607397386647	27.027027567289064	26.829776750085333	23.047121708759136
55-59	23.085297029381355	26.898416366363136	26.819871856058203	23.196414748197306
60-64	23.04150210566453	26.964542278455454	26.891669844195317	23.1022857716847
65-69	23.008274234632047	26.975634060641738	26.894640812661073	23.12145089206514
70-74	23.000013493044335	26.960804205432098	26.859363997859102	23.17981830366446
75-79	22.970393762015682	26.954237590522084	26.918313609334387	23.157055038127844
80-84	22.969379284978594	27.022954666869232	26.868224430306174	23.139441617846003
85-89	22.96436437016718	26.96739830617317	26.836288393333234	23.231948930326414
90-94	22.337745092600063	26.60809444967417	28.173134911126553	22.881025546599222
95-99	22.935238413973593	27.133139283446155	26.963884659628544	22.96773764295171
100-104	22.991615607003837	27.143269902995137	26.93638941020643	22.928725079794596
105-109	22.96771903316205	27.081718266822357	26.971808757101417	22.978753942914175
110-114	22.958487146423074	26.939718035484738	27.051050054658116	23.050744763434075
115-119	22.975247608254715	27.047672654494164	26.878553054825822	23.0985266824253
120-124	22.936768884619475	27.020049102007317	26.906066560696264	23.137115452676948
125-129	22.897205983261916	27.019528853639173	27.019936778746978	23.06332838435193
130-134	22.970890771981683	27.10031485208162	26.896376513718334	23.03241786221836
135-139	23.047901634661503	27.00284609994136	26.864334789562843	23.084917475834292
140-144	23.00527129185646	27.106779064693033	26.921424071664546	22.966525571785965
145-149	22.905980063384103	27.200362444685418	26.957282787123045	22.936374704807434
150-154	22.887644683555482	27.229429362046677	26.9821457318113	22.90078022258654
155-159	22.79181334587278	27.2326694895972	27.13607999262569	22.839437171904333
160-164	22.74244629998683	27.424462999868304	27.096736799917775	22.736353900227087
165-169	22.685321802605078	27.378019312467906	27.13704416163192	22.799614723295097
170-174	22.62834425562221	27.50162402655894	27.210790609585096	22.65924110823375
175-178	22.57255377248549	27.591825868301946	27.184223190105293	22.65139716910727
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	4.0
12	11.5
13	18.0
14	31.0
15	57.5
16	122.5
17	278.0
18	555.0
19	1096.0
20	2075.5
21	3822.0
22	6603.5
23	10700.0
24	16385.5
25	23717.5
26	32539.0
27	43277.5
28	56069.0
29	70127.5
30	85753.5
31	102636.0
32	120274.0
33	139260.0
34	159097.0
35	179392.0
36	200937.0
37	223599.5
38	245573.0
39	266790.5
40	286490.0
41	304328.0
42	319797.0
43	329965.5
44	334601.0
45	334268.0
46	329169.0
47	319091.0
48	304305.5
49	286432.5
50	266943.5
51	247277.0
52	228275.5
53	209737.0
54	193416.0
55	180017.5
56	168526.0
57	158972.0
58	150304.0
59	141737.0
60	132938.0
61	123149.5
62	112840.0
63	102102.5
64	90324.5
65	78101.5
66	65950.0
67	54309.5
68	43602.0
69	33968.0
70	25552.0
71	18652.5
72	13253.0
73	9139.0
74	6166.5
75	3994.5
76	2558.5
77	1601.0
78	911.5
79	537.0
80	304.0
81	156.5
82	89.5
83	43.5
84	18.0
85	10.5
86	6.5
87	2.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	858.0
92-93	2000.0
94-95	3138.0
96-97	4260.0
98-99	5369.0
100-101	6495.0
102-103	7370.0
104-105	8104.0
106-107	8851.0
108-109	9888.0
110-111	10291.0
112-113	10871.0
114-115	12096.0
116-117	11779.0
118-119	12129.0
120-121	12408.0
122-123	13051.0
124-125	13484.0
126-127	14021.0
128-129	14848.0
130-131	15462.0
132-133	17192.0
134-135	81479.0
136-137	85742.0
138-139	86361.0
140-141	86414.0
142-143	88420.0
144-145	94820.0
146-147	80710.0
148-149	75773.0
150-151	76071.0
152-153	74936.0
154-155	69656.0
156-157	67562.0
158-159	69318.0
160-161	71417.0
162-163	74928.0
164-165	77453.0
166-167	78578.0
168-169	82536.0
170-171	83993.0
172-173	88872.0
174-175	95135.0
176-177	187915.0
178-179	5912070.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.26435139859765
#Duplication Level	Percentage of total
1	89.18281805248142
2	9.183773557747934
3	1.1908498393188243
4	0.24841911275132106
5	0.08400747543308529
6	0.030926338024817767
7	0.019708713159068178
8	0.01148124645341554
9	0.01890404789275043
>10	0.029111616737367996
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.748067871012493E-5	1.2493559570041643E-5	0.0	0.0	0.0	0.0
2	2.623647509708745E-4	1.2493559570041643E-5	1.2493559570041643E-5	0.0	0.0	0.0
3	2.873518701109578E-4	2.4987119140083287E-5	1.2493559570041643E-5	1.2493559570041643E-5	0.0	0.0
4	2.873518701109578E-4	2.4987119140083287E-5	2.4987119140083287E-5	2.4987119140083287E-5	0.0	0.0
5	2.9984542968099947E-4	2.4987119140083287E-5	2.4987119140083287E-5	2.4987119140083287E-5	1.2493559570041643E-5	0.0
6	3.3732610839112435E-4	2.4987119140083287E-5	2.4987119140083287E-5	3.748067871012493E-5	1.2493559570041643E-5	0.0
7	3.3732610839112435E-4	2.4987119140083287E-5	2.4987119140083287E-5	3.748067871012493E-5	1.2493559570041643E-5	0.0
8	3.3732610839112435E-4	2.4987119140083287E-5	2.4987119140083287E-5	4.997423828016657E-5	1.2493559570041643E-5	0.0
9	3.3732610839112435E-4	2.4987119140083287E-5	3.748067871012493E-5	4.997423828016657E-5	1.2493559570041643E-5	0.0
10-14	3.49819667961166E-4	2.4987119140083287E-5	4.997423828016657E-5	8.745491699029151E-5	1.2493559570041643E-5	0.0
15-19	3.6231322753120765E-4	2.9984542968099948E-5	7.246264550624154E-5	1.1494074804438313E-4	1.2493559570041643E-5	0.0
20-24	3.8979905858529934E-4	3.748067871012493E-5	1.0744461230235813E-4	1.74909833980583E-4	1.2493559570041643E-5	0.0
25-29	4.072900419833576E-4	4.997423828016657E-5	1.499227148404997E-4	2.1488922460471626E-4	1.2493559570041643E-5	0.0
30-34	5.172333661997242E-4	5.2472950194174904E-5	2.223853603467413E-4	2.5986603905686623E-4	2.248840722607496E-5	2.4987119140083286E-6
35-39	8.995362890429983E-4	6.746522167822488E-5	2.773570224549245E-4	3.248325488210827E-4	4.747552636615825E-5	2.248840722607496E-5
40-44	0.001269345652316231	7.496135742024987E-5	2.9984542968099947E-4	3.972951943273243E-4	7.746006933425818E-5	4.747552636615825E-5
45-49	0.0015242142675450806	9.245234081830817E-5	3.173364130790577E-4	4.597629921775325E-4	8.74549169902915E-5	5.9969085936199896E-5
50-54	0.0017915764423439714	9.994847656033315E-5	3.64811939445216E-4	5.272282138557573E-4	9.49510527323165E-5	7.496135742024987E-5
55-59	0.0020039669550346796	9.994847656033315E-5	3.748067871012493E-4	5.871972997919573E-4	1.3493044335644976E-4	8.745491699029151E-5
60-64	0.002111411567337038	1.0994332421636647E-4	3.823029228432743E-4	6.096857070180322E-4	2.1488922460471626E-4	9.994847656033315E-5
65-69	0.002158887093703196	1.2493559570041644E-4	4.022926181553409E-4	6.521638095561738E-4	2.498711914008329E-4	1.2243688378640812E-4
70-74	0.004165352760651884	1.474240029264914E-4	4.247810253814158E-4	7.046367597503486E-4	3.0734156542302447E-4	1.5242142675450806E-4
75-79	0.040514114973731044	1.4992271484049973E-4	4.472694326074909E-4	7.44616150374482E-4	3.323286845631077E-4	1.74909833980583E-4
80-84	0.05597864300952858	1.82405969722608E-4	4.7225655174757417E-4	7.895929648266319E-4	4.597629921775325E-4	1.8740339355062465E-4
85-89	0.056063599214604874	2.3487891991678294E-4	4.997423828016658E-4	8.495620507628319E-4	6.746522167822487E-4	1.8740339355062465E-4
90-94	0.05612106958862707	2.8235444628294117E-4	5.497166210818323E-4	8.720504579889068E-4	6.746522167822487E-4	1.8740339355062465E-4
95-99	0.056226015489015416	3.073415654230244E-4	5.846985878779489E-4	9.070324247850232E-4	6.746522167822487E-4	1.8740339355062465E-4
100-104	0.05630347555834967	3.123389892510411E-4	6.471663857281571E-4	9.345182558391149E-4	6.746522167822487E-4	1.8740339355062465E-4
105-109	0.05639342918725397	3.323286845631077E-4	6.746522167822487E-4	9.620040868932065E-4	6.746522167822487E-4	1.8740339355062465E-4
110-114	0.056510868647212366	3.49819667961166E-4	7.07135471664357E-4	9.969860536893234E-4	6.796496406102653E-4	1.8740339355062465E-4
115-119	0.05660332098803067	3.49819667961166E-4	7.770994052565903E-4	9.994847656033315E-4	6.946419120943154E-4	1.8740339355062465E-4
120-124	0.056958138079819844	3.79804210929266E-4	8.395672031067985E-4	9.994847656033315E-4	6.99639335922332E-4	1.8740339355062465E-4
125-129	0.05753534053195577	3.997939062413326E-4	8.870427294729568E-4	0.0010319680204854398	7.07135471664357E-4	1.8740339355062465E-4
130-134	0.057995103524133305	4.072900419833576E-4	9.095311366990316E-4	0.001064451275367548	7.196290312343986E-4	2.0489437694868294E-4
135-139	0.05874471709833581	4.247810253814158E-4	9.445131034951481E-4	0.001086939682593623	7.246264550624153E-4	2.123905126907079E-4
140-144	0.05939937961980598	4.2977844920943253E-4	9.744976464632482E-4	0.0011294177851317645	7.29623878890432E-4	2.123905126907079E-4
145-149	0.059706721185229	4.3727458495145747E-4	9.919886298613066E-4	0.0011843894472399479	7.396187265464653E-4	2.2238536034674124E-4
150-154	0.05987913230729559	4.3727458495145747E-4	0.0010119783251733732	0.0011993817187239979	7.59608421858532E-4	2.2488407226074959E-4
155-159	0.06027892621353692	4.3727458495145747E-4	0.001049459003883498	0.001224368837864081	7.746006933425819E-4	2.2488407226074959E-4
160-164	0.0607211982223164	4.4726943260749077E-4	0.0010769448349375898	0.0012493559570041645	7.870942529126237E-4	2.3238020800277456E-4
165-167	0.06134337748890447	4.622617040915408E-4	0.0010994332421636647	0.00129100115557097	7.870942529126236E-4	2.3737763183079123E-4
>>END_MODULE
