##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293525
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7573520
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-169
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.05359370543684	41.0	40.0	41.0	38.0	41.0
2	40.13885854926111	41.0	40.0	41.0	38.0	41.0
3	40.19702542542966	41.0	40.0	41.0	38.0	41.0
4	40.201702114736605	41.0	40.0	41.0	38.0	41.0
5	40.18951557532033	41.0	40.0	41.0	38.0	41.0
6	40.17226335970592	41.0	40.0	41.0	38.0	41.0
7	40.161688884428905	41.0	40.0	41.0	38.0	41.0
8	40.15568956046858	41.0	40.0	41.0	38.0	41.0
9	40.13173728992595	41.0	40.0	41.0	38.0	41.0
10-14	40.05637280947301	41.0	40.0	41.0	38.0	41.0
15-19	39.89996052033929	41.0	40.0	41.0	38.0	41.0
20-24	39.70178131172823	40.6	39.6	41.0	37.4	41.0
25-29	39.52977033136507	40.0	39.0	41.0	36.8	41.0
30-34	39.318741377853364	40.0	38.8	41.0	35.4	41.0
35-39	38.854359267553264	40.0	37.6	41.0	35.0	41.0
40-44	38.416065105789656	39.4	36.4	41.0	35.0	41.0
45-49	37.676568121560386	38.0	35.0	40.4	34.6	41.0
50-54	36.88680893428683	36.6	35.0	39.2	34.0	41.0
55-59	36.14917649917079	35.4	35.0	37.8	34.0	40.0
60-64	35.210423977225915	34.8	34.4	36.0	33.0	39.0
65-69	35.29684323801878	35.0	35.0	36.0	34.0	37.2
70-74	35.07566315266878	35.0	35.0	35.0	33.8	36.6
75-79	34.97207111092332	35.0	35.0	35.0	33.0	36.0
80-84	34.935415157020785	35.0	35.0	35.0	33.0	36.0
85-89	38.8780362631907	39.8	38.8	39.8	36.8	40.0
90-94	39.965123798266646	41.0	40.0	41.0	38.0	41.0
95-99	39.825032758340654	41.0	40.0	41.0	37.6	41.0
100-104	39.658399222104	41.0	39.6	41.0	36.8	41.0
105-109	39.42821989817704	40.2	39.0	41.0	35.8	41.0
110-114	39.160159209763336	40.0	38.0	41.0	35.0	41.0
115-119	38.71199647276973	39.6	37.6	40.6	34.8	40.8
120-124	38.36839240864019	39.6	36.8	40.6	34.8	40.8
125-129	37.98261650069387	38.8	35.4	41.0	34.6	41.0
130-134	37.20323400893805	37.2	35.0	39.8	34.0	41.0
135-139	36.47984659928042	36.0	35.0	39.0	34.0	40.8
140-144	35.93720482967532	35.0	35.0	37.0	34.0	39.2
145-149	35.36999061362065	35.0	35.0	36.0	34.0	37.8
150-154	34.978947589834505	35.0	35.0	35.2	34.0	36.4
155-159	34.747956824243765	35.0	35.0	35.0	33.2	36.0
160-164	34.62895259865361	35.0	35.0	35.0	33.0	35.8
165-169	34.42074329491792	35.0	34.8	35.0	32.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	5.0
30	168.0
31	1793.0
32	12068.0
33	51125.0
34	159360.0
35	429297.0
36	1174419.0
37	2627370.0
38	2620557.0
39	495006.0
40	2352.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.89867855369762	26.847159577052675	27.390169960599565	22.86399190865014
2	22.61793723394142	27.00057569003581	27.556354244789745	22.825132831233034
3	22.47495220188235	26.973917016129885	27.494295915241523	23.056834866746243
4	22.90879274102399	27.08118549894897	27.057009158224975	22.953012601802065
5	22.801418627005674	27.277619917818928	27.09877309362093	22.822188361554467
6	22.568845134098808	27.155655494406826	27.2656043689064	23.009895002587967
7	22.956406004077365	27.233611319439312	26.934463763217103	22.87551891326622
8	22.936679377620976	27.340417665761755	26.971051769850746	22.751851186766523
9	22.645454161341092	27.132165756477832	27.079270933462908	23.143109148718167
10-14	22.831845693944164	27.222028858443633	27.01810518754819	22.928020260064013
15-19	22.752463847722062	27.072713876770642	27.19044512987356	22.984377145633733
20-24	22.77136391004447	27.058590985433455	27.122254909209985	23.047790195312086
25-29	22.83633501991148	27.163202843591883	27.009287623192385	22.991174513304248
30-34	22.78171840834909	27.085054241620803	27.056216924230743	23.07701042579936
35-39	22.76686138017725	27.13136560014366	27.097252004352008	23.004521015327086
40-44	22.92719369593003	27.092284697208168	26.95500110912759	23.025520497734213
45-49	22.958552958201732	27.055855137373374	26.9591893861771	23.026402518247792
50-54	22.954208875133357	27.051149267447634	26.97249099494027	23.02215086247874
55-59	22.974149404768195	27.1111398662709	26.913213406711805	23.001497322249097
60-64	22.89396476143194	27.042498072230615	27.00125172971089	23.06228543662656
65-69	22.8913873601707	27.09720711109233	27.000190136158615	23.01121539257835
70-74	22.911908861401304	27.119133507272707	26.948087547137924	23.020870084188065
75-79	22.872608773727407	27.079223399423253	26.949716908386062	23.09845091846328
80-84	22.83744942906337	27.09991655135261	27.004130179889934	23.05850383969409
85-89	22.77384360244642	27.099950881492358	27.154063104078418	22.972142411982805
90-94	22.763768019253288	27.19858779225352	27.076474619238528	22.961169569254665
95-99	22.740709845130848	27.203292571716858	27.15105512272087	22.90494246043143
100-104	22.769460786963574	27.08308460190114	27.18005875523964	22.967395855895646
105-109	22.780698350199362	27.065532530203328	27.067182681035607	23.086586438561703
110-114	22.769338800219703	27.129230331377897	27.089957588629655	23.011473279772744
115-119	22.793326286378676	27.132282671235448	27.051934689968142	23.022456352417734
120-124	22.730656828120903	27.123602485885172	27.11206892513718	23.033671760856745
125-129	22.82778623789995	27.095164525657793	27.03904273987805	23.038006496564208
130-134	22.940373433458014	27.145475907081046	26.954700805973296	22.959449853487644
135-139	22.877170886822277	27.117061852291968	26.992473906432473	23.01329335445328
140-144	22.819912264609762	27.211547554130988	27.085911856194105	22.882628325065145
145-149	22.841099614981417	27.22237465869666	27.072809939196407	22.863715787125514
150-154	22.753648201087394	27.251308011414423	27.123222633757493	22.87182115374069
155-159	22.727718206433607	27.299582861171025	27.167409825525972	22.805289106869395
160-164	22.687030995028305	27.402488738162802	27.15079410207238	22.75968616473651
165-169	22.632797314204932	27.389349512991878	27.216323621421285	22.761529551381905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	3.0
13	5.5
14	8.0
15	21.0
16	45.5
17	100.0
18	234.0
19	518.0
20	1080.0
21	2041.5
22	3583.0
23	6152.0
24	10151.5
25	15632.0
26	22864.5
27	31997.0
28	43343.5
29	56358.0
30	71034.0
31	87345.0
32	104317.5
33	121814.0
34	139705.0
35	158041.0
36	176497.5
37	195672.5
38	215247.5
39	235263.5
40	256166.0
41	276056.5
42	295569.0
43	314613.5
44	330228.5
45	341689.5
46	349386.0
47	352424.5
48	350299.0
49	341012.0
50	325393.0
51	304268.0
52	277604.0
53	248886.5
54	219122.5
55	190167.5
56	163930.5
57	141092.5
58	121563.5
59	104552.0
60	90623.5
61	79545.0
62	69949.0
63	61145.0
64	53254.5
65	45741.0
66	38692.0
67	32237.0
68	26071.0
69	20494.5
70	15737.0
71	11801.0
72	8441.5
73	5948.0
74	4171.0
75	2813.0
76	1815.0
77	1081.5
78	637.0
79	385.0
80	211.5
81	107.5
82	59.0
83	25.5
84	13.0
85	3.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	776.0
92-93	1793.0
94-95	2774.0
96-97	3707.0
98-99	4249.0
100-101	5019.0
102-103	5795.0
104-105	6748.0
106-107	7752.0
108-109	7562.0
110-111	7938.0
112-113	8601.0
114-115	8903.0
116-117	9451.0
118-119	9810.0
120-121	10526.0
122-123	10708.0
124-125	11487.0
126-127	12074.0
128-129	23333.0
130-131	75671.0
132-133	75954.0
134-135	79684.0
136-137	73954.0
138-139	63808.0
140-141	68831.0
142-143	74539.0
144-145	64975.0
146-147	62728.0
148-149	61761.0
150-151	64219.0
152-153	64915.0
154-155	68816.0
156-157	70973.0
158-159	72710.0
160-161	74222.0
162-163	76117.0
164-165	80085.0
166-167	94136.0
168-169	6046416.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.12047403236868
#Duplication Level	Percentage of total
1	80.98239629333155
2	13.361331035861468
3	3.110359844220029
4	0.8628861268750257
5	0.34518791994982057
6	0.2103378687419661
7	0.13596081884451858
8	0.1337214819728678
9	0.12092924722377076
>10	0.7289344517119758
>50	0.0057350534791606725
>100	0.0022198577878446905
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3203899903875609E-5	0.0	0.0	0.0	0.0	0.0
2	1.3203899903875609E-5	0.0	0.0	0.0	1.3203899903875609E-5	0.0
3	1.3203899903875609E-5	0.0	0.0	0.0	1.3203899903875609E-5	0.0
4	1.3203899903875609E-5	0.0	0.0	2.6407799807751217E-5	1.3203899903875609E-5	0.0
5	1.3203899903875609E-5	0.0	0.0	2.6407799807751217E-5	1.3203899903875609E-5	0.0
6	1.3203899903875609E-5	1.3203899903875609E-5	0.0	5.2815599615502434E-5	2.6407799807751217E-5	0.0
7	1.3203899903875609E-5	1.3203899903875609E-5	0.0	5.2815599615502434E-5	2.6407799807751217E-5	0.0
8	2.6407799807751217E-5	1.3203899903875609E-5	0.0	6.601949951937804E-5	2.6407799807751217E-5	0.0
9	2.6407799807751217E-5	1.3203899903875609E-5	0.0	6.601949951937804E-5	2.6407799807751217E-5	0.0
10-14	4.2252479692401945E-5	1.3203899903875609E-5	5.281559961550243E-6	6.601949951937804E-5	3.9611699711626826E-5	0.0
15-19	8.186417940402877E-5	1.3203899903875609E-5	2.1126239846200972E-5	6.866027950015316E-5	8.450495938480389E-5	0.0
20-24	9.770885928867951E-5	1.3203899903875609E-5	5.017481963472731E-5	1.0034963926945462E-4	1.0827197921178E-4	0.0
25-29	1.0563119923100487E-4	1.584467988465073E-5	6.601949951937804E-5	1.0563119923100487E-4	1.3732055900030632E-4	0.0
30-34	1.1883509913488048E-4	2.6407799807751217E-5	6.601949951937804E-5	1.2147587911565561E-4	2.0069927853890926E-4	0.0
35-39	1.927769385965839E-4	4.2252479692401945E-5	6.601949951937804E-5	1.4788367892340683E-4	2.9840813782758876E-4	0.0
40-44	2.799226779621629E-4	5.2815599615502434E-5	8.186417940402877E-5	1.6372835880805757E-4	3.5386451742386624E-4	0.0
45-49	3.2745671761611514E-4	6.073793955782779E-5	9.242729932712926E-5	1.822138186734834E-4	4.2780635688556974E-4	0.0
50-54	3.7499075727006725E-4	1.0034963926945463E-4	9.242729932712926E-5	2.0069927853890923E-4	4.753403965395219E-4	2.3767019826976095E-5
55-59	4.5157337671254585E-4	1.1883509913488048E-4	9.242729932712926E-5	2.191847384043351E-4	5.096705362895986E-4	2.6407799807751217E-5
60-64	4.7798117652029707E-4	1.1883509913488048E-4	9.242729932712926E-5	2.455925382120863E-4	5.915347156936273E-4	2.6407799807751217E-5
65-69	4.885442964433975E-4	1.2147587911565558E-4	1.1355353917333023E-4	3.03689697789139E-4	6.892435749823069E-4	2.6407799807751217E-5
70-74	0.007322882886689412	1.3996133898108146E-4	1.452428989426317E-4	3.353790575584405E-4	7.631854144440101E-4	3.168935976930146E-5
75-79	0.013605298460953427	1.6636913878883267E-4	1.584467988465073E-4	3.7499075727006725E-4	8.556127137711395E-4	3.9611699711626826E-5
80-84	0.013668677180492032	1.7957303869270825E-4	1.584467988465073E-4	3.987577770970434E-4	0.0010431080924061733	3.9611699711626826E-5
85-89	0.013732055900030632	1.8485459865425852E-4	1.584467988465073E-4	4.1988401694324437E-4	0.0010774382321562497	3.9611699711626826E-5
90-94	0.013745259799934509	1.8485459865425852E-4	1.7693225871193312E-4	4.3308791684712E-4	0.0010853605720985752	3.9611699711626826E-5
95-99	0.013782230719665362	1.8749537863503362E-4	1.9541771857735902E-4	4.4893259673177064E-4	0.001130253831771752	3.9611699711626826E-5
100-104	0.013827123979338538	1.9805849855813416E-4	2.0069927853890923E-4	4.7798117652029707E-4	0.0011566616315795033	3.9611699711626826E-5
105-109	0.013850890999165514	2.0069927853890923E-4	2.2182551838511024E-4	5.096705362895984E-4	0.0011857102113680296	3.9611699711626826E-5
110-114	0.013861454119088613	2.3502941828898585E-4	2.3502941828898585E-4	5.228744361934741E-4	0.0012068364512142307	3.9611699711626826E-5
115-119	0.013898425038819464	2.3767019826976095E-4	2.6407799807751217E-4	5.387191160781249E-4	0.0012438073709450821	3.9611699711626826E-5
120-124	0.014075357297531399	2.455925382120863E-4	2.746411180006127E-4	5.888939357128521E-4	0.0012860598506374843	3.9611699711626826E-5
125-129	0.014191551616685505	2.6143721809673707E-4	2.772818979813878E-4	6.364279753668043E-4	0.0013283123303298862	3.9611699711626826E-5
130-134	0.014352639195512787	2.6407799807751217E-4	2.931265778660385E-4	6.601949951937805E-4	0.001431302749580116	3.9611699711626826E-5
135-139	0.01442658103497449	2.6407799807751217E-4	3.03689697789139E-4	6.892435749823067E-4	0.0014867591291763936	3.9611699711626826E-5
140-144	0.014468833514666893	2.6407799807751217E-4	3.2217515765456487E-4	7.182921547708332E-4	0.0015316523888495707	3.9611699711626826E-5
145-149	0.014542775354128595	2.6407799807751217E-4	3.3009749759689024E-4	7.262144947131584E-4	0.0015818272084842978	3.9611699711626826E-5
150-154	0.014621998753551848	2.746411180006127E-4	3.3273827757766535E-4	7.367776146362589E-4	0.0016187981282151497	4.753403965395219E-5
155-158	0.0146695327932058	2.772818979813878E-4	3.433013975007658E-4	7.493213195449409E-4	0.0016603904129123578	6.601949951937804E-5
>>END_MODULE
