##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293526
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2346940
Total Bases	385.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-169
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.09780309679839	41.0	40.0	41.0	38.0	41.0
2	40.169447876809805	41.0	40.0	41.0	38.0	41.0
3	40.22457540456936	41.0	40.0	41.0	39.0	41.0
4	40.2218288494806	41.0	40.0	41.0	39.0	41.0
5	40.21124655934962	41.0	40.0	41.0	39.0	41.0
6	40.19512556776057	41.0	40.0	41.0	38.0	41.0
7	40.18252746129002	41.0	40.0	41.0	38.0	41.0
8	40.17326390960144	41.0	40.0	41.0	38.0	41.0
9	40.146739584309785	41.0	40.0	41.0	38.0	41.0
10-14	40.07480694010073	41.0	40.0	41.0	38.0	41.0
15-19	39.914557082839785	41.0	40.0	41.0	38.0	41.0
20-24	39.70820447050202	40.4	39.6	41.0	37.4	41.0
25-29	39.525450075417346	40.0	39.0	41.0	36.8	41.0
30-34	39.299789854022684	40.0	38.6	41.0	35.4	41.0
35-39	38.8241328708872	40.0	37.4	41.0	35.0	41.0
40-44	38.3808963160541	39.4	36.4	41.0	35.0	41.0
45-49	37.61975406273701	38.0	35.0	40.4	34.6	41.0
50-54	36.8162100437165	36.4	35.0	39.2	34.0	41.0
55-59	36.08795623237066	35.2	35.0	37.6	34.0	39.6
60-64	35.16315534270156	34.8	34.4	36.0	33.0	38.8
65-69	35.27627642802969	35.0	35.0	36.0	34.0	37.0
70-74	35.06911007524692	35.0	35.0	35.0	34.0	36.4
75-79	34.97175598864904	35.0	35.0	35.0	33.2	36.0
80-84	34.934699140156965	35.0	35.0	35.0	33.0	36.0
85-89	38.91814200618678	39.8	38.8	39.8	36.8	40.0
90-94	40.009040405201866	41.0	40.0	41.0	38.0	41.0
95-99	39.86040765479548	41.0	40.0	41.0	37.6	41.0
100-104	39.6866329501359	41.0	39.6	41.0	37.0	41.0
105-109	39.458019147214955	40.2	39.0	41.0	35.8	41.0
110-114	39.19240392136874	40.0	38.2	41.0	35.0	41.0
115-119	38.72036405736439	39.6	37.6	40.6	34.8	40.8
120-124	38.39082966328603	39.6	36.8	40.6	34.8	40.8
125-129	38.01066196908548	38.8	35.4	41.0	34.6	41.0
130-134	37.21639017574254	37.2	35.0	39.8	34.0	41.0
135-139	36.47995974500115	36.0	35.0	38.8	34.0	40.8
140-144	35.93087230843171	35.0	35.0	37.0	34.0	39.2
145-149	35.36882973961562	35.0	35.0	36.0	34.0	37.6
150-154	34.974630611830975	35.0	35.0	35.2	34.0	36.4
155-159	34.75000503543832	35.0	35.0	35.0	33.4	36.0
160-164	34.632144963908836	35.0	35.0	35.0	33.0	35.8
165-169	34.41760650174252	35.0	34.8	35.0	32.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	48.0
31	513.0
32	3206.0
33	13855.0
34	45149.0
35	127847.0
36	369103.0
37	836450.0
38	809883.0
39	140437.0
40	448.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.95533758851952	26.833579043350063	27.348078774915425	22.863004593214995
2	22.683281208722846	26.966390278405072	27.45903176050517	22.891296752366912
3	22.55975866447374	26.96059549881974	27.396823097309685	23.082822739396832
4	22.938805423231955	27.015731122227237	26.976147664618612	23.069315789922197
5	22.836800259060734	27.25361534593982	27.061450228808575	22.84813416619087
6	22.54885084407782	27.15719191798682	27.221360580159697	23.072596657775655
7	22.98891322317571	27.198692765899423	26.90217900755878	22.910215003366087
8	23.002974085404826	27.255958822978005	26.939887683536863	22.80117940808031
9	22.680000340869388	27.052630233410312	27.077002394607447	23.190367031112853
10-14	22.86371189719379	27.16227939359336	27.003459824281833	22.970548884931016
15-19	22.766666382608843	27.040367457199586	27.1485679224863	23.04439823770527
20-24	22.83346826079917	27.02628102976642	27.04950275678117	23.090747952653242
25-29	22.876801281668897	27.132572626483846	26.952644720359274	23.03798137148798
30-34	22.818129138367407	27.041679804340973	27.02635772537858	23.113833331913046
35-39	22.815231748574742	27.124860456594547	27.009476168968956	23.050431625861762
40-44	22.942197073636308	27.059191969117236	26.9160183046861	23.082592652560354
45-49	22.990506787561678	27.014793731411967	26.91708352152164	23.077615959504715
50-54	23.00835982172531	27.025573725787623	26.896614314809923	23.069452137677146
55-59	23.01129129845671	27.08651265051514	26.845236776398202	23.056959274629943
60-64	22.97746001175999	26.985163659914612	26.92095238906832	23.116423939257075
65-69	22.95172437301337	27.044969193929113	26.9311188185467	23.07218761451081
70-74	22.96033984677921	27.07955891501274	26.898787357154426	23.06131388105363
75-79	22.930513775384117	27.024210248238134	26.888535710329194	23.156740266048555
80-84	22.889089623083674	27.03665198087723	26.959163847392777	23.115094548646322
85-89	22.837260432733686	27.031223635883322	27.11500933129948	23.016506600083513
90-94	22.80131550754922	27.183999749089473	27.01230842290629	23.00237632045502
95-99	22.78218651299744	27.148416527095176	27.10829567667985	22.96110128322753
100-104	22.83234384880058	27.047285735749167	27.10709298421343	23.013277431236826
105-109	22.8349351134415	27.00052481649396	27.032457144265653	23.13208292579889
110-114	22.80084659494684	27.09501920659307	27.063987000963397	23.040147197496697
115-119	22.8544549561815	27.067070503199766	27.005574475535155	23.072900065083584
120-124	22.79056435863778	27.08992061302169	27.05605334664088	23.063461681699653
125-129	22.865874600391642	27.065942839254685	26.970115656990483	23.098066903363186
130-134	23.002129865873165	27.07163949787123	26.90191594833132	23.02431468792428
135-139	22.936690773226854	27.10129350149964	26.9385359296462	23.023479795627303
140-144	22.886770274697543	27.165517666295756	27.01420638176672	22.933505677239978
145-149	22.85797092966448	27.189047810331473	27.04258686618989	22.910394393814162
150-154	22.80529518841787	27.23108783301913	27.07022386861459	22.89339310994841
155-159	22.755062674504522	27.2518922427706	27.14828180849249	22.84476327423239
160-164	22.736970010551918	27.33846194587151	27.1059836302673	22.81858441330927
165-169	22.680086746388636	27.370221134039173	27.146746677890533	22.802945441681654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	7.5
16	15.5
17	41.5
18	78.5
19	159.0
20	320.0
21	575.5
22	1070.0
23	1891.5
24	2978.5
25	4620.5
26	6870.0
27	9618.0
28	13002.5
29	16971.5
30	21585.5
31	26428.0
32	31371.0
33	36726.0
34	42423.5
35	48130.5
36	54121.0
37	60094.0
38	66059.0
39	72623.5
40	78884.5
41	84942.0
42	91372.5
43	97162.5
44	102121.0
45	106312.5
46	108753.5
47	109835.0
48	109077.5
49	106133.5
50	101357.5
51	94494.5
52	86499.5
53	77815.0
54	68659.5
55	59829.5
56	51627.5
57	44115.0
58	37779.0
59	32793.0
60	28604.5
61	24984.5
62	21978.0
63	19343.0
64	16802.0
65	14343.5
66	12093.0
67	10089.0
68	8226.5
69	6572.0
70	5011.5
71	3674.5
72	2701.0
73	1905.5
74	1292.0
75	818.5
76	519.0
77	332.0
78	201.5
79	121.0
80	74.0
81	43.0
82	19.5
83	9.5
84	5.5
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	215.0
92-93	496.0
94-95	759.0
96-97	1059.0
98-99	1299.0
100-101	1542.0
102-103	1705.0
104-105	1962.0
106-107	2129.0
108-109	2261.0
110-111	2346.0
112-113	2489.0
114-115	2602.0
116-117	2686.0
118-119	2984.0
120-121	3124.0
122-123	3160.0
124-125	3367.0
126-127	3574.0
128-129	7317.0
130-131	22533.0
132-133	22981.0
134-135	23386.0
136-137	22578.0
138-139	19691.0
140-141	19812.0
142-143	21735.0
144-145	19165.0
146-147	18958.0
148-149	18819.0
150-151	19978.0
152-153	20101.0
154-155	20807.0
156-157	21234.0
158-159	21713.0
160-161	22685.0
162-163	23234.0
164-165	25272.0
166-167	32151.0
168-169	1883031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.66662342337699
#Duplication Level	Percentage of total
1	92.12665806558358
2	6.188229785740922
3	0.8758285597126982
4	0.27765881558383454
5	0.19340593819656787
6	0.12649243272037244
7	0.06910835286365163
8	0.053202342992087315
9	0.02833268240817236
>10	0.061083024198091404
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.2608673421561695E-5	0.0	0.0	0.0	0.0	0.0
2	8.521734684312339E-5	0.0	0.0	0.0	0.0	0.0
3	8.521734684312339E-5	0.0	4.2608673421561695E-5	0.0	0.0	0.0
4	8.521734684312339E-5	4.2608673421561695E-5	4.2608673421561695E-5	0.0	0.0	0.0
5	8.521734684312339E-5	4.2608673421561695E-5	4.2608673421561695E-5	0.0	0.0	0.0
6	8.521734684312339E-5	4.2608673421561695E-5	4.2608673421561695E-5	0.0	0.0	0.0
7	8.521734684312339E-5	4.2608673421561695E-5	4.2608673421561695E-5	0.0	0.0	0.0
8	8.521734684312339E-5	4.2608673421561695E-5	4.2608673421561695E-5	0.0	0.0	0.0
9	8.521734684312339E-5	8.521734684312339E-5	4.2608673421561695E-5	0.0	4.2608673421561695E-5	0.0
10-14	8.521734684312339E-5	8.521734684312339E-5	4.2608673421561695E-5	0.0	4.2608673421561695E-5	0.0
15-19	8.521734684312339E-5	8.521734684312339E-5	4.2608673421561695E-5	3.408693873724936E-5	8.521734684312339E-5	0.0
20-24	8.521734684312339E-5	8.521734684312339E-5	4.2608673421561695E-5	1.0226081621174806E-4	1.1930428558037273E-4	0.0
25-29	8.521734684312339E-5	8.521734684312339E-5	8.521734684312339E-5	2.1304336710780848E-4	1.6191295900193443E-4	0.0
30-34	9.373908152743573E-5	8.521734684312339E-5	8.521734684312339E-5	2.7269550989799486E-4	2.812172445823072E-4	0.0
35-39	1.533912243176221E-4	8.521734684312339E-5	8.521734684312339E-5	3.9199979547836756E-4	4.1756499953130466E-4	0.0
40-44	2.7269550989799486E-4	8.521734684312339E-5	8.521734684312339E-5	4.3460846889992926E-4	6.30608366639113E-4	0.0
45-49	3.323476526881812E-4	8.521734684312339E-5	1.4486948963330978E-4	4.8573887700580326E-4	7.243474481665488E-4	0.0
50-54	4.0052153016267996E-4	8.521734684312339E-5	2.1304336710780848E-4	5.453910197959896E-4	8.095647950096721E-4	0.0
55-59	4.5165193826855396E-4	8.521734684312339E-5	2.1304336710780848E-4	5.53912754480302E-4	8.521734684312339E-4	0.0
60-64	4.6869540763717855E-4	1.2782602026468508E-4	2.1304336710780848E-4	5.53912754480302E-4	8.947821418527956E-4	0.0
65-69	4.8573887700580326E-4	1.2782602026468508E-4	2.3860857116074545E-4	5.53912754480302E-4	9.544342846429819E-4	0.0
70-74	0.008018952337937911	1.2782602026468508E-4	2.5565204052937015E-4	5.79477958533239E-4	0.0010396516314861053	0.0
75-79	0.014111992637221233	1.533912243176221E-4	2.5565204052937015E-4	5.965214279018637E-4	0.001082260304907667	0.0
80-84	0.014180166514695733	1.7043469368624678E-4	2.8121724458230715E-4	6.135648972704883E-4	0.0012015645904880398	0.0
85-89	0.014188688249380044	1.7043469368624678E-4	2.9826071395093186E-4	6.391301013234254E-4	0.001261216733278226	0.0
90-94	0.014188688249380044	1.7043469368624678E-4	2.9826071395093186E-4	6.391301013234254E-4	0.001354955814805662	0.0
95-99	0.014188688249380044	1.7043469368624678E-4	2.9826071395093186E-4	6.391301013234254E-4	0.0013634775494899742	0.0
100-104	0.014188688249380044	1.7043469368624678E-4	3.238259180038689E-4	6.817387747449871E-4	0.001389042753542911	0.0
105-109	0.014222775188117293	1.7043469368624678E-4	3.4086938737249356E-4	6.817387747449871E-4	0.0014060862229115358	0.0
110-114	0.014231296922801607	1.7043469368624678E-4	3.4086938737249356E-4	7.073039787979241E-4	0.0014316514269644728	0.0
115-119	0.01435060120838198	2.1304336710780848E-4	3.4086938737249356E-4	7.584343869037981E-4	0.0014913035697546593	0.0
120-124	0.014444340289909412	2.1304336710780848E-4	3.4086938737249356E-4	7.839995909567352E-4	0.0015253905084919085	0.0
125-129	0.014699992330438783	2.5565204052937015E-4	3.4086938737249356E-4	8.606952031155462E-4	0.0016532165287565937	2.5565204052937016E-5
130-134	0.01490451396286228	2.5565204052937015E-4	3.4086938737249356E-4	9.28869080590045E-4	0.0017469556102840292	4.2608673421561695E-5
135-139	0.014955644370968154	2.5565204052937015E-4	3.4086938737249356E-4	9.373908152743571E-4	0.001832172957127153	4.2608673421561695E-5
140-144	0.01508347039123284	2.8121724458230715E-4	3.4086938737249356E-4	9.544342846429819E-4	0.001866259895864402	4.2608673421561695E-5
145-149	0.015168687738075965	2.9826071395093186E-4	3.579128567411182E-4	0.0010226081621174806	0.002019651120182024	4.2608673421561695E-5
150-154	0.01527094855428771	2.9826071395093186E-4	3.8347806079405526E-4	0.0010652168355390424	0.0020793032629722106	4.2608673421561695E-5
155-158	0.015381731105183772	2.9826071395093186E-4	3.8347806079405526E-4	0.0010652168355390424	0.0021304336710780847	4.2608673421561695E-5
>>END_MODULE
