##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293527
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7510541
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-169
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.078478234790275	41.0	40.0	41.0	38.0	41.0
2	40.154208598288726	41.0	40.0	41.0	38.0	41.0
3	40.20758770906117	41.0	40.0	41.0	39.0	41.0
4	40.20862398594189	41.0	40.0	41.0	38.0	41.0
5	40.199440359888854	41.0	40.0	41.0	38.0	41.0
6	40.18561112974418	41.0	40.0	41.0	38.0	41.0
7	40.17169788434681	41.0	40.0	41.0	38.0	41.0
8	40.16591641001627	41.0	40.0	41.0	38.0	41.0
9	40.143076910171985	41.0	40.0	41.0	38.0	41.0
10-14	40.06708187865561	41.0	40.0	41.0	38.0	41.0
15-19	39.915768517873744	41.0	40.0	41.0	38.0	41.0
20-24	39.71788053616909	40.6	39.8	41.0	37.4	41.0
25-29	39.546060610014635	40.0	39.0	41.0	36.8	41.0
30-34	39.33221124816441	40.0	38.8	41.0	35.4	41.0
35-39	38.86638246166289	40.0	37.4	41.0	35.0	41.0
40-44	38.41029063019561	39.4	36.4	41.0	35.0	41.0
45-49	37.65479599938273	38.0	35.0	40.4	34.6	41.0
50-54	36.85140359396214	36.4	35.0	39.2	34.0	41.0
55-59	36.115325966531564	35.4	35.0	37.6	34.0	39.8
60-64	35.192299675882204	35.0	34.4	36.0	33.0	39.0
65-69	35.27107874119854	35.0	35.0	36.0	34.0	37.2
70-74	35.06166570424155	35.0	35.0	35.0	34.0	36.4
75-79	34.95850842169692	35.0	35.0	35.0	33.2	36.0
80-84	34.92788269180609	35.0	35.0	35.0	33.0	36.0
85-89	38.89600392301966	39.8	38.8	39.8	36.8	40.0
90-94	39.99254151315137	41.0	40.0	41.0	38.0	41.0
95-99	39.85597929136904	41.0	40.0	41.0	37.8	41.0
100-104	39.69400171999073	41.0	39.8	41.0	37.0	41.0
105-109	39.460492457633094	40.4	39.0	41.0	35.8	41.0
110-114	39.1933086309466	40.0	38.2	41.0	35.0	41.0
115-119	38.758748911343886	39.6	37.6	40.6	34.8	40.8
120-124	38.42557300324335	39.8	36.8	40.6	34.8	40.8
125-129	38.04844770333458	39.0	35.4	41.0	34.6	41.0
130-134	37.28491198612057	37.2	35.0	40.2	34.0	41.0
135-139	36.56024692149677	36.2	35.0	39.0	34.0	41.0
140-144	36.00830985752428	35.0	35.0	37.2	34.0	39.4
145-149	35.42735694141101	35.0	35.0	36.2	34.0	37.8
150-154	35.019194924836725	35.0	35.0	35.4	34.0	36.6
155-159	34.779742585892166	35.0	35.0	35.0	33.6	36.0
160-164	34.650118935296916	35.0	35.0	35.0	33.0	36.0
165-169	34.44021916336148	35.0	34.8	35.0	32.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	161.0
31	1786.0
32	11668.0
33	49837.0
34	153333.0
35	412212.0
36	1144669.0
37	2597023.0
38	2632260.0
39	505134.0
40	2456.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.96957036783369	26.77104086110441	27.34749733740885	22.911891433653047
2	22.589677627750117	26.988215629207	27.528323192696767	22.893783550346107
3	22.52534138353016	26.95803138548874	27.459979247833143	23.05664798314795
4	22.908403003192447	27.054096369356085	27.039436972649504	22.998063654801964
5	22.82566595402382	27.25867550686429	27.097195794550622	22.818462744561277
6	22.568973393527845	27.17592514307558	27.265971918667375	22.9891295447292
7	22.97191374096753	27.255639773486358	26.862019660101716	22.9104268254444
8	22.93826769602882	27.326380349964136	26.98620512157513	22.74914683243191
9	22.663227589064487	27.092256070501445	27.07978027148777	23.164736068946297
10-14	22.842074891808725	27.199931403077354	27.016738208339454	22.941255496774467
15-19	22.763534610888883	27.052626435299405	27.17352318561339	23.01031576819832
20-24	22.790118581337882	27.049769117830525	27.09678304132818	23.06332925950341
25-29	22.83735352752884	27.150398353460826	26.999911191484077	23.01233692752626
30-34	22.799425500772845	27.079588540958632	27.03146684107044	23.089519117198083
35-39	22.768825840908132	27.115386228502047	27.085833097775513	23.029954832814305
40-44	22.933011084021775	27.086056783392838	26.939167231761335	23.041764900824056
45-49	22.967610455758113	27.041593941102242	26.93555364387199	23.055241959267647
50-54	22.943484364175628	27.049185937471083	26.965373067000098	23.041956631353187
55-59	22.97926607417495	27.107538591427698	26.877573799277577	23.035621535119773
60-64	22.914668863401452	27.033799562508214	26.991427115569973	23.060104458520364
65-69	22.901479400751555	27.06589312274575	26.995780996335682	23.036846480167007
70-74	22.92496639057027	27.10335780072301	26.933974529930666	23.03770127877606
75-79	22.906565585621593	27.06793824838983	26.939808996449123	23.08568716953945
80-84	22.863719138208552	27.09761919946912	26.977302966590557	23.061358695731773
85-89	22.808506071666475	27.063605671016244	27.139560252716816	22.98832800460047
90-94	22.76847260334443	27.19083692772245	27.06797853047444	22.97271193845868
95-99	22.758095080717595	27.167983752535335	27.14202139142904	22.931899775318033
100-104	22.782351706825267	27.070202671204456	27.162492639462666	22.98495298250761
105-109	22.780985563027485	27.047740946154093	27.06730634871372	23.1039671421047
110-114	22.772933315600504	27.121662960457176	27.078796411316524	23.026607312625796
115-119	22.804615733877682	27.118376065561474	27.049105916955124	23.02790228360572
120-124	22.769746201172495	27.1047129968938	27.094770158908503	23.030770643025203
125-129	22.854734844322845	27.081214504486063	27.00004091967068	23.06400973152041
130-134	22.964297636970027	27.119227003821063	26.93822836777929	22.978246991429625
135-139	22.904424147193357	27.115553084305322	26.97699401144468	23.003028757056637
140-144	22.829743304176983	27.19833115088398	27.067815949589118	22.904109595349915
145-149	22.84977403834571	27.211802066061345	27.050344083395494	22.888079812197446
150-154	22.776889360890483	27.24225056079308	27.10113505699865	22.87972502131779
155-159	22.73656680726484	27.291983385072328	27.155212280929447	22.816237526733392
160-164	22.735624938727984	27.3649016065376	27.126204384638974	22.773269070095438
165-169	22.66005240837664	27.378748742215038	27.191142857695965	22.77005599171236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	3.5
14	8.5
15	18.5
16	48.5
17	109.5
18	226.5
19	489.0
20	1045.5
21	2053.5
22	3621.0
23	6092.5
24	9912.5
25	15306.5
26	22444.0
27	31575.5
28	42606.0
29	55466.5
30	70018.5
31	86100.5
32	102980.5
33	119933.5
34	137326.5
35	155648.0
36	174177.0
37	193258.5
38	212784.5
39	232620.0
40	253466.5
41	274234.0
42	293424.0
43	310654.0
44	326400.5
45	338944.0
46	347505.0
47	350686.5
48	347520.0
49	338585.5
50	323383.5
51	301350.5
52	275566.5
53	247572.0
54	217980.0
55	189862.5
56	163628.5
57	140263.5
58	120210.5
59	103914.0
60	90625.5
61	79342.5
62	70010.5
63	61397.0
64	52940.5
65	45487.0
66	38603.0
67	31894.0
68	25860.5
69	20503.5
70	15844.5
71	11827.0
72	8552.0
73	5960.5
74	4123.0
75	2777.5
76	1765.0
77	1089.0
78	646.0
79	364.5
80	197.0
81	106.5
82	55.0
83	24.5
84	12.5
85	5.0
86	1.5
87	1.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	655.0
92-93	1672.0
94-95	2491.0
96-97	3384.0
98-99	4160.0
100-101	4704.0
102-103	5282.0
104-105	6282.0
106-107	6705.0
108-109	7258.0
110-111	7475.0
112-113	7929.0
114-115	8251.0
116-117	8768.0
118-119	9197.0
120-121	9902.0
122-123	10023.0
124-125	10639.0
126-127	11155.0
128-129	23317.0
130-131	71912.0
132-133	72897.0
134-135	73729.0
136-137	69633.0
138-139	60901.0
140-141	62033.0
142-143	69247.0
144-145	60595.0
146-147	59483.0
148-149	60144.0
150-151	60162.0
152-153	64104.0
154-155	64871.0
156-157	66173.0
158-159	68310.0
160-161	70638.0
162-163	72775.0
164-165	76756.0
166-167	93614.0
168-169	6063315.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.285321472881
#Duplication Level	Percentage of total
1	81.25677164383217
2	13.118004296305527
3	3.150358176104446
4	0.8854816888531604
5	0.35758316208750085
6	0.18978164733791203
7	0.15678032001469508
8	0.1107878489601492
9	0.09679194443595873
>10	0.6725292662064417
>50	0.0029396738096587162
>100	0.002190332052359172
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.3314620078633483E-5	0.0	0.0
3	0.0	1.3314620078633483E-5	0.0	2.6629240157266966E-5	1.3314620078633483E-5	0.0
4	0.0	1.3314620078633483E-5	1.3314620078633483E-5	2.6629240157266966E-5	2.6629240157266966E-5	0.0
5	0.0	1.3314620078633483E-5	1.3314620078633483E-5	2.6629240157266966E-5	2.6629240157266966E-5	0.0
6	0.0	1.3314620078633483E-5	1.3314620078633483E-5	2.6629240157266966E-5	3.994386023590045E-5	0.0
7	0.0	1.3314620078633483E-5	1.3314620078633483E-5	2.6629240157266966E-5	3.994386023590045E-5	0.0
8	0.0	1.3314620078633483E-5	1.3314620078633483E-5	5.325848031453393E-5	3.994386023590045E-5	0.0
9	0.0	1.3314620078633483E-5	1.3314620078633483E-5	5.325848031453393E-5	5.325848031453393E-5	0.0
10-14	5.325848031453393E-6	1.3314620078633483E-5	3.994386023590045E-5	6.391017637744072E-5	5.592140433026062E-5	0.0
15-19	1.3314620078633483E-5	1.3314620078633483E-5	6.657310039316741E-5	9.320234055043439E-5	9.053941653470768E-5	0.0
20-24	1.597754409436018E-5	1.3314620078633483E-5	6.657310039316741E-5	1.3048327677060812E-4	1.2515742873915473E-4	0.0
25-29	2.6629240157266966E-5	1.3314620078633483E-5	9.320234055043438E-5	1.7042713700650858E-4	1.571125169278751E-4	0.0
30-34	5.059555629880724E-5	2.1303392125813573E-5	1.1716865669197466E-4	2.1037099724240902E-4	2.1569684527386244E-4	0.0
35-39	1.3314620078633483E-4	2.6629240157266966E-5	1.1983158070770134E-4	2.3700023739967602E-4	2.3966316141540268E-4	1.3314620078633483E-5
40-44	2.2368561732104253E-4	2.6629240157266966E-5	1.1983158070770134E-4	2.556407055097629E-4	3.035733377928434E-4	3.195508818872036E-5
45-49	2.8759579369848327E-4	2.6629240157266966E-5	1.1983158070770134E-4	2.822699456670298E-4	3.355284259815638E-4	5.325848031453393E-5
50-54	3.541688940916506E-4	2.6629240157266966E-5	1.3314620078633483E-4	3.115621098400235E-4	3.834610582646444E-4	5.325848031453393E-5
55-59	3.9411275432755106E-4	2.6629240157266966E-5	1.4646082086496831E-4	3.3286550196583707E-4	4.7932632283080535E-4	5.325848031453393E-5
60-64	4.3139369054772487E-4	2.6629240157266966E-5	1.677642129907819E-4	3.435171980287439E-4	5.672028153497863E-4	5.325848031453393E-5
65-69	4.3938246259490497E-4	3.195508818872036E-5	1.730900610222353E-4	3.86123982280371E-4	6.817085480260343E-4	5.325848031453393E-5
70-74	0.00791687309875547	4.526970826735384E-5	1.837417570851421E-4	4.3405661456345155E-4	7.935513566865556E-4	5.325848031453393E-5
75-79	0.013975025234533704	6.391017637744072E-5	1.8640468110086878E-4	4.7932632283080535E-4	8.707761531426298E-4	5.325848031453393E-5
80-84	0.01405757587902123	7.98877204718009E-5	1.8640468110086878E-4	5.219331070824326E-4	0.0010438662141648652	5.325848031453393E-5
85-89	0.014105508511304312	9.852818858188778E-5	1.9439345314804885E-4	5.325848031453393E-4	0.0010917988464479455	5.325848031453393E-5
90-94	0.01413213775146158	1.0651696062906786E-4	2.2102269330531583E-4	5.325848031453393E-4	0.001097124694479399	5.325848031453393E-5
95-99	0.014142789447524487	1.0651696062906786E-4	2.3167438936822263E-4	5.485623472396995E-4	0.0011370685547152995	5.325848031453393E-5
100-104	0.01418007038374466	1.1450573267624796E-4	2.449890094468561E-4	5.592140433026063E-4	0.001158371946841113	5.325848031453393E-5
105-109	0.014201373775870474	1.3048327677060815E-4	2.529777814940362E-4	5.725286633812398E-4	0.0011929899590455602	5.325848031453393E-5
110-114	0.014206699623901925	1.597754409436018E-4	2.6362947755694297E-4	5.885062074756E-4	0.0012782035275488144	5.325848031453393E-5
115-119	0.01423599178807492	1.597754409436018E-4	2.7960702165130317E-4	6.57742231884494E-4	0.001318147387784715	5.325848031453393E-5
120-124	0.014374463836892708	1.597754409436018E-4	2.7960702165130317E-4	6.896973200732145E-4	0.0013820575641621555	5.325848031453393E-5
125-129	0.01453423927783631	1.597754409436018E-4	2.8493286968275653E-4	7.110007121990281E-4	0.0014725969806968632	5.325848031453393E-5
130-134	0.014704666414842818	1.597754409436018E-4	3.062362618085701E-4	7.482816484192017E-4	0.0015897656373888378	5.325848031453393E-5
135-139	0.014829823843581974	1.597754409436018E-4	3.062362618085701E-4	7.642591925135619E-4	0.0016376982696719184	5.325848031453393E-5
140-144	0.014899059867990866	1.730900610222353E-4	3.222138059029303E-4	7.935513566865556E-4	0.0017335635342380796	5.592140433026062E-5
145-149	0.014981610512478394	1.7841590905368868E-4	3.3286550196583707E-4	8.121918247966425E-4	0.0017761703184897067	6.657310039316741E-5
150-154	0.015114756713264732	1.9971930117950224E-4	3.5683181810737736E-4	8.121918247966425E-4	0.001832091722819967	6.657310039316741E-5
155-158	0.015215282094858413	1.9971930117950224E-4	3.761380172213959E-4	8.25506444875276E-4	0.0018773614310873214	6.657310039316741E-5
>>END_MODULE
