##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293528
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7563065
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-169
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.05919478412522	41.0	40.0	41.0	38.0	41.0
2	40.1438147100415	41.0	40.0	41.0	38.0	41.0
3	40.20264482719638	41.0	40.0	41.0	38.0	41.0
4	40.20819588354721	41.0	40.0	41.0	38.0	41.0
5	40.19418053395019	41.0	40.0	41.0	38.0	41.0
6	40.17630616687811	41.0	40.0	41.0	38.0	41.0
7	40.16816502304291	41.0	40.0	41.0	38.0	41.0
8	40.157141846592616	41.0	40.0	41.0	38.0	41.0
9	40.13566867400981	41.0	40.0	41.0	38.0	41.0
10-14	40.058069869821296	41.0	40.0	41.0	38.0	41.0
15-19	39.90636428485012	41.0	40.0	41.0	38.0	41.0
20-24	39.708279857438754	40.6	39.6	41.0	37.4	41.0
25-29	39.54427880759983	40.0	39.0	41.0	36.8	41.0
30-34	39.332554248839585	40.0	38.8	41.0	35.4	41.0
35-39	38.874927691352646	40.0	37.6	41.0	35.0	41.0
40-44	38.43432169365198	39.4	36.4	41.0	35.0	41.0
45-49	37.695073571362926	38.0	35.0	40.6	34.6	41.0
50-54	36.9110747296235	36.6	35.0	39.2	34.0	41.0
55-59	36.17028482500151	35.4	35.0	37.8	34.0	40.0
60-64	35.2148794966062	34.8	34.4	36.0	33.0	39.0
65-69	35.31348808981544	35.0	35.0	36.0	34.0	37.2
70-74	35.09326073490047	35.0	35.0	35.0	34.0	36.8
75-79	34.989699334859615	35.0	35.0	35.0	33.2	36.0
80-84	34.95297845516335	35.0	35.0	35.0	33.0	36.0
85-89	38.886166018671	39.8	38.8	39.8	36.8	40.0
90-94	39.971370975364245	41.0	40.0	41.0	38.0	41.0
95-99	39.83673716380186	41.0	40.0	41.0	37.6	41.0
100-104	39.671800552458365	41.0	39.6	41.0	36.8	41.0
105-109	39.440192121448845	40.2	39.0	41.0	35.8	41.0
110-114	39.172642541286294	40.0	38.0	41.0	35.0	41.0
115-119	38.71932420907232	39.6	37.6	40.6	34.6	40.8
120-124	38.383487351001676	39.6	36.8	40.6	34.8	40.8
125-129	38.020667140353126	39.0	35.4	41.0	34.6	41.0
130-134	37.241230659414256	37.2	35.0	39.8	34.0	41.0
135-139	36.51582891219973	36.0	35.0	39.0	34.0	41.0
140-144	35.96873110470936	35.0	35.0	37.0	34.0	39.4
145-149	35.39386127798009	35.0	35.0	36.0	34.0	37.8
150-154	34.99998972071826	35.0	35.0	35.4	34.0	36.6
155-159	34.766773062078634	35.0	35.0	35.0	33.6	36.0
160-164	34.644945895225305	35.0	35.0	35.0	33.0	36.0
165-169	34.4335953993066	35.0	34.8	35.0	32.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	165.0
31	1787.0
32	11877.0
33	50612.0
34	156457.0
35	419042.0
36	1153612.0
37	2600847.0
38	2646036.0
39	519200.0
40	3427.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.914122779587377	26.861186569201774	27.382906268820907	22.841784382389942
2	22.58678194620832	27.02697914139307	27.558192346621375	22.828046565777235
3	22.474790313186517	26.990195112695716	27.503968298566782	23.031046275550985
4	22.88022118016968	27.060219633177816	27.078651313984476	22.980907872668023
5	22.802422033924078	27.267899984992855	27.14780317239109	22.781874808691978
6	22.546837823025452	27.179853670436522	27.250724408688804	23.02258409784922
7	22.955415562341457	27.23799147567818	26.907649213645524	22.898943748334837
8	22.90929669386684	27.329277217635973	26.993579983776417	22.76784610472077
9	22.61416502436512	27.1265419509154	27.122231529148564	23.13706149557091
10-14	22.828834606075713	27.22762266356299	27.044141495544466	22.89940123481684
15-19	22.73924129965827	27.077387276190272	27.19523367840948	22.98813774574197
20-24	22.765429095214703	27.083287000706726	27.111280942316373	23.040002961762195
25-29	22.837746336967882	27.17679670874176	27.012712438674	22.972744515616352
30-34	22.770390046892363	27.112029316156878	27.055607746330356	23.061972890620403
35-39	22.76702104239485	27.142630666270883	27.09964809240698	22.990700198927286
40-44	22.907001328165236	27.129670312234524	26.957843149569655	23.005485210030592
45-49	22.959099254072257	27.071289219383942	26.960699134543997	23.008912391999807
50-54	22.926575402961628	27.08289033612695	26.98045303061656	23.01008123029486
55-59	22.934582738611926	27.138743353389135	26.91211301238321	23.01456089561573
60-64	22.88442053585418	27.061081717531188	27.01747770249231	23.037020044122322
65-69	22.875564338003176	27.11179396183955	27.01390243241331	22.998739267743964
70-74	22.88255621232926	27.13187841172858	26.98545100432166	23.0001143716205
75-79	22.857357433791723	27.08621967416649	26.978184109220273	23.078238782821515
80-84	22.82214155239972	27.11488265670069	27.019862978831995	23.043112812067594
85-89	22.781557477028162	27.09394141131935	27.168308086734676	22.956193024917805
90-94	22.74131097667417	27.21673008468596	27.092334421416282	22.949624517223583
95-99	22.732068399979592	27.211780004133573	27.155854756585924	22.900296839300914
100-104	22.754355099912228	27.0903483875751	27.193978391681746	22.961318120830924
105-109	22.750335671634	27.073194221611203	27.099158213885683	23.077311892869112
110-114	22.743859918176028	27.144217144379933	27.122342781077773	22.989580156366262
115-119	22.769680810881884	27.13346796227044	27.082611348180997	23.01423987866668
120-124	22.731369885746037	27.128262369289985	27.139609659128432	23.000758085835542
125-129	22.80316659846084	27.117127458589014	27.05265915922604	23.027046783724103
130-134	22.93664361204114	27.14010194791026	26.976359330054095	22.946895109994504
135-139	22.863838251249312	27.141511295908433	27.00873811760606	22.985912335236193
140-144	22.803108456234753	27.19989781829582	27.116567511365115	22.880426214104315
145-149	22.803031438970216	27.22743607632489	27.110264655405107	22.859267829299785
150-154	22.741641231721662	27.2752909216137	27.136064623550343	22.8470032231143
155-159	22.68608551304178	27.30223645464351	27.20498866221576	22.806689370098947
160-164	22.693114493054676	27.40602615210604	27.16340910792978	22.737450246909503
165-169	22.618128167390662	27.42326932968549	27.215327482733652	22.743275020190197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.5
12	3.0
13	4.5
14	10.5
15	24.0
16	52.5
17	116.0
18	258.5
19	532.0
20	1053.5
21	2014.5
22	3661.0
23	6233.0
24	9954.0
25	15405.0
26	22802.0
27	31999.0
28	43329.5
29	56732.5
30	71747.5
31	88027.5
32	104546.5
33	121629.5
34	139371.0
35	157800.0
36	176700.0
37	196212.5
38	216223.0
39	236010.5
40	256080.0
41	276263.5
42	295840.0
43	313658.0
44	329371.5
45	342590.5
46	350789.0
47	352588.5
48	349300.0
49	340127.5
50	324064.5
51	302067.5
52	276219.5
53	248199.0
54	218843.0
55	189941.5
56	163806.5
57	140800.5
58	120752.0
59	103787.0
60	90114.5
61	78885.0
62	69227.0
63	61067.5
64	53251.0
65	45439.0
66	38236.5
67	31686.0
68	25580.5
69	20131.5
70	15523.5
71	11720.0
72	8475.5
73	5932.5
74	4044.0
75	2667.5
76	1728.5
77	1067.0
78	637.0
79	360.0
80	196.0
81	109.0
82	58.0
83	29.0
84	13.0
85	6.0
86	3.5
87	2.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	780.0
92-93	2693.0
94-95	2883.0
96-97	3829.0
98-99	4581.0
100-101	5648.0
102-103	6617.0
104-105	6657.0
106-107	7056.0
108-109	7560.0
110-111	8171.0
112-113	8499.0
114-115	8755.0
116-117	9402.0
118-119	10122.0
120-121	10770.0
122-123	10750.0
124-125	11249.0
126-127	11995.0
128-129	24495.0
130-131	75305.0
132-133	85049.0
134-135	78322.0
136-137	73270.0
138-139	64986.0
140-141	66902.0
142-143	72436.0
144-145	64204.0
146-147	62515.0
148-149	62318.0
150-151	68334.0
152-153	64781.0
154-155	66912.0
156-157	68972.0
158-159	73352.0
160-161	73765.0
162-163	76867.0
164-165	79459.0
166-167	95270.0
168-169	6027534.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.32595629156397
#Duplication Level	Percentage of total
1	81.33168215051921
2	13.082249331054557
3	3.1187250753809805
4	0.860933778083741
5	0.344199050783518
6	0.20004913322471018
7	0.16088840364108709
8	0.11388622316701777
9	0.1030661291032112
>10	0.6780452048426017
>50	0.0041566876068279985
>100	0.0021188325924945584
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.3222152659007956E-5	1.3222152659007956E-5	0.0
2	0.0	1.3222152659007956E-5	1.3222152659007956E-5	2.6444305318015912E-5	1.3222152659007956E-5	0.0
3	0.0	1.3222152659007956E-5	1.3222152659007956E-5	2.6444305318015912E-5	1.3222152659007956E-5	0.0
4	0.0	1.3222152659007956E-5	2.6444305318015912E-5	3.966645797702387E-5	2.6444305318015912E-5	0.0
5	0.0	1.3222152659007956E-5	2.6444305318015912E-5	3.966645797702387E-5	3.966645797702387E-5	0.0
6	0.0	1.3222152659007956E-5	3.966645797702387E-5	5.2888610636031824E-5	3.966645797702387E-5	0.0
7	0.0	1.3222152659007956E-5	3.966645797702387E-5	6.611076329503978E-5	5.2888610636031824E-5	0.0
8	0.0	1.3222152659007956E-5	3.966645797702387E-5	6.611076329503978E-5	5.2888610636031824E-5	0.0
9	1.3222152659007956E-5	1.3222152659007956E-5	3.966645797702387E-5	6.611076329503978E-5	5.2888610636031824E-5	0.0
10-14	2.379987478621432E-5	1.3222152659007956E-5	4.759974957242864E-5	1.0313279074026206E-4	6.34663327632382E-5	0.0
15-19	2.6444305318015912E-5	1.3222152659007956E-5	7.933291595404774E-5	1.269326655264764E-4	9.784392967665888E-5	0.0
20-24	2.9088735849817504E-5	1.3222152659007956E-5	1.1106608233566683E-4	1.3222152659007956E-4	1.2428823499467477E-4	0.0
25-29	3.966645797702387E-5	1.3222152659007956E-5	1.1899937393107158E-4	1.3222152659007956E-4	2.0097672041692092E-4	0.0
30-34	7.404405489044455E-5	1.3222152659007956E-5	1.1899937393107158E-4	1.3751038765368273E-4	2.961762195617782E-4	5.2888610636031825E-6
35-39	1.6659912350350025E-4	1.586658319080955E-5	1.3751038765368273E-4	1.4544367924908751E-4	3.675758439204212E-4	2.6444305318015912E-5
40-44	2.8030963637096863E-4	2.6444305318015912E-5	1.5073254031269069E-4	1.5866583190809547E-4	4.4690875987446894E-4	3.7022027445222275E-5
45-49	3.1204280275258777E-4	2.6444305318015912E-5	1.771768456307066E-4	1.6659912350350025E-4	5.262416758285166E-4	3.966645797702387E-5
50-54	3.75509135515826E-4	3.437759691342068E-5	1.9039899828971455E-4	1.798212761625082E-4	5.791302864645486E-4	4.495531904062705E-5
55-59	3.966645797702386E-4	5.024418010423023E-5	2.0626558148052412E-4	2.0891001201232568E-4	6.39952188695985E-4	5.2888610636031824E-5
60-64	4.4426432934266733E-4	5.2888610636031824E-5	2.115544425441273E-4	2.3270988679854003E-4	7.034185214592233E-4	5.2888610636031824E-5
65-69	4.759974957242863E-4	6.0821902231436594E-5	2.2477659520313522E-4	2.565097615847544E-4	8.039068816676837E-4	5.2888610636031824E-5
70-74	0.007481093974466701	7.933291595404774E-5	2.4857646998934955E-4	2.961762195617782E-4	8.673732144309219E-4	5.2888610636031824E-5
75-79	0.013322641019216413	1.2428823499467477E-4	2.7502077530736546E-4	3.226205248797941E-4	9.361284082577634E-4	5.2888610636031824E-5
80-84	0.013423129379424876	1.9568785935331775E-4	2.776652058391671E-4	3.4906483019781E-4	0.0011873493087789142	5.2888610636031824E-5
85-89	0.0134601514068701	2.3270988679854003E-4	2.9088735849817503E-4	3.6757584392042115E-4	0.0012032158919697239	5.2888610636031824E-5
90-94	0.013473373559529106	2.3799874786214315E-4	3.04109511157183E-4	4.0459787136564346E-4	0.001208504753033327	5.2888610636031824E-5
95-99	0.013507751156442527	2.4328760892574638E-4	3.2262052487979417E-4	4.231088850882546E-4	0.0012455267804785495	5.2888610636031824E-5
100-104	0.01351304001750613	2.5122090052115116E-4	3.384871080706037E-4	4.416198988108657E-4	0.0012561045026057557	5.5533041167833416E-5
105-109	0.01351304001750613	2.5122090052115116E-4	3.569981217932148E-4	4.786419262560879E-4	0.0012666822247329622	6.611076329503978E-5
110-114	0.013539484322824147	2.5122090052115116E-4	3.781535660476276E-4	4.997973705105007E-4	0.0013222152659007956	6.611076329503978E-5
115-119	0.01359766179452378	2.5915419211655595E-4	3.834424271112307E-4	5.024418010423023E-4	0.0013724594460050258	6.611076329503978E-5
120-124	0.013690216863136836	2.6973191424376234E-4	3.834424271112307E-4	5.024418010423023E-4	0.001441214639831867	6.611076329503978E-5
125-129	0.013822438389726915	2.776652058391671E-4	3.834424271112307E-4	5.288861063603182E-4	0.0015443474305721291	6.611076329503978E-5
130-134	0.01398110422163501	2.776652058391671E-4	3.834424271112307E-4	5.817747169963501E-4	0.0016210359159943752	6.611076329503978E-5
135-139	0.01407630372077987	2.882429279663734E-4	3.966645797702386E-4	6.08219022314366E-4	0.0016950799708848197	7.668848542224615E-5
140-144	0.014163569928329322	2.9088735849817503E-4	4.0988673242924663E-4	6.214411749733739E-4	0.0017321019983300421	7.933291595404774E-5
145-149	0.01421116967790175	2.9088735849817503E-4	4.0988673242924663E-4	6.425966192277867E-4	0.0017929239005614786	7.933291595404774E-5
150-154	0.014261413858005981	2.935317890299766E-4	4.0988673242924663E-4	6.478854802913898E-4	0.0018775456775791295	7.933291595404774E-5
155-158	0.014355952249517887	3.1733166381619094E-4	4.1980334692350257E-4	6.511910184561417E-4	0.0019502675172036734	8.263845411879972E-5
>>END_MODULE
