##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293534
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7772982
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.939515748267524	41.0	40.0	41.0	38.0	41.0
2	40.05920945140488	41.0	40.0	41.0	38.0	41.0
3	40.081676633240626	41.0	40.0	41.0	38.0	41.0
4	40.08009062673759	41.0	40.0	41.0	38.0	41.0
5	40.065436791182584	41.0	40.0	41.0	38.0	41.0
6	40.042377815875554	41.0	40.0	41.0	38.0	41.0
7	40.0320463883745	41.0	40.0	41.0	38.0	41.0
8	40.00439252271522	41.0	40.0	41.0	38.0	41.0
9	39.96775819112922	41.0	40.0	41.0	38.0	41.0
10-14	39.87486038176854	41.0	40.0	41.0	38.0	41.0
15-19	39.65976208358646	40.2	39.4	41.0	37.2	41.0
20-24	39.34658991877249	40.0	38.6	41.0	36.0	41.0
25-29	39.07030830638744	40.0	38.0	41.0	35.0	41.0
30-34	38.63806891615084	40.0	37.0	41.0	35.0	41.0
35-39	38.05403491221258	39.0	35.4	41.0	34.2	41.0
40-44	37.54835724564909	37.8	35.0	40.4	34.0	41.0
45-49	36.79625299531119	36.4	35.0	39.4	34.0	41.0
50-54	36.131239465111335	35.2	35.0	38.0	33.4	40.4
55-59	35.31314154593437	34.8	34.6	36.4	32.4	38.8
60-64	35.138193141319505	35.0	34.8	36.0	33.0	37.8
65-69	35.104464026804635	35.0	35.0	35.2	33.0	37.0
70-74	34.98784204054506	35.0	35.0	35.0	33.0	36.2
75-79	34.93492013747105	35.0	35.0	35.0	33.0	36.0
80-84	34.81056690984232	35.0	34.8	35.0	32.6	36.2
85-89	39.81022472456517	41.0	40.0	41.0	37.6	41.0
90-94	39.718587388581895	41.0	40.0	41.0	37.0	41.0
95-99	39.52208801258379	40.6	39.0	41.0	36.0	41.0
100-104	39.287613389156796	40.0	38.6	41.0	35.2	41.0
105-109	38.943788960000795	40.0	38.0	41.0	35.0	41.0
110-114	38.58717059696875	40.0	37.0	41.0	35.0	41.0
115-119	37.842698285434	39.2	35.8	40.4	34.0	40.6
120-124	37.692443231358155	38.8	35.0	40.6	34.0	41.0
125-129	37.08020271914988	37.0	35.0	40.0	33.6	41.0
130-134	36.358198659915196	35.6	35.0	39.0	33.0	40.8
135-139	35.83087144158411	35.0	35.0	37.2	33.0	39.6
140-144	35.437144390448964	35.0	35.0	36.2	33.0	38.4
145-149	35.0161296497937	35.0	35.0	35.4	33.0	36.8
150-154	34.73535983554431	35.0	35.0	35.0	33.0	36.0
155-159	34.57388688858208	35.0	35.0	35.0	33.0	36.0
160-164	34.478989594237866	35.0	34.8	35.0	33.0	35.0
165-168	34.233849390798014	35.0	33.75	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	183.0
31	2432.0
32	16220.0
33	71239.0
34	240074.0
35	739284.0
36	1827660.0
37	2724212.0
38	1907633.0
39	243323.0
40	716.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.863364150335098	25.908319355428844	26.468207439564377	23.760109054671684
2	23.565897875487167	25.954582166792616	26.464926845321397	24.01459311239882
3	23.850035932155766	26.1020673918967	26.038513919111097	24.009382756836438
4	23.975779179727937	26.28457135241018	26.108924477118308	23.63072499074358
5	23.585967393208936	26.17528253635477	26.294438865290054	23.944311205146235
6	23.9015862895347	26.133278064969147	26.045422464634548	23.919713180861603
7	24.054127489295617	26.13085943078216	26.038899871374976	23.776113208547248
8	23.707362245274723	25.983451396130853	26.01889467903052	24.290291679563904
9	24.01834971443392	26.072722154766343	25.731012885402283	24.177915245397454
10-14	23.733861727712736	26.22343908682665	26.182878076907933	23.859821108552676
15-19	23.771484354395778	25.93783441155531	26.24245109534539	24.048230138703524
20-24	23.726011458665415	26.029176447340284	26.078848503701668	24.165963590292634
25-29	23.762604879311443	26.037273725836496	26.166886788107835	24.033234606744234
30-34	23.703641665450917	26.13750552876618	26.092783953442833	24.066068852340067
35-39	23.75827964094089	26.01580191488929	26.089426168747075	24.136492275422743
40-44	23.859828827597955	26.03160537358764	26.01386186150952	24.094703937304885
45-49	24.03722278013766	25.999092754878372	25.93098504537898	24.032699419604985
50-54	23.971484817538492	25.98186127280367	25.884120148483554	24.16253376117428
55-59	23.93657929479317	26.021238695779818	26.00647988120904	24.035702128217974
60-64	23.95520792406312	26.041364819833625	25.91882754906675	24.084599707036503
65-69	23.892009527360283	26.013465617185272	25.982131439388382	24.112393416066062
70-74	23.88086837200961	26.03326496832232	25.984946317899617	24.100920341768447
75-79	23.87846260289809	26.06774337056229	25.95549301413537	24.09830101240425
80-84	23.855830362144157	26.0193501027019	25.916902933777543	24.207916601376407
85-89	23.677983558948164	26.155318512251796	26.23179109381702	23.93490683498302
90-94	23.831971398821945	26.198477535280063	25.98725100311114	23.98230006278685
95-99	23.63454035178499	26.237466851208147	26.195896056521022	23.932096740485836
100-104	23.705529252839273	26.03386980542878	26.20548146885388	24.05511947287807
105-109	23.71784251245338	26.207624550513064	26.02925233804206	24.0452805989915
110-114	23.7123786714275	26.097277289124456	26.069248300553994	24.121095738894052
115-119	23.625744495254924	26.216018385708406	26.154704130637818	24.003532988398852
120-124	23.705305061937363	26.18767954082909	26.052369699405297	24.05464569782825
125-129	23.784332187271602	26.14804854247057	25.970261354610912	24.097357915646917
130-134	23.91469962564132	26.15256302684289	25.96097973796596	23.97175760954983
135-139	23.825069695731372	26.30153137210392	25.953447506494282	23.91995142567043
140-144	23.733833083426426	26.294451788608676	26.112273520085417	23.859441607879482
145-149	23.622554021015535	26.4301794522667	26.194995576044878	23.752270950672884
150-154	23.620410229429847	26.490651405726684	26.203471730510557	23.68546663433291
155-159	23.513456058313714	26.540853095791135	26.22626804357859	23.719422802316565
160-164	23.44131878413093	26.650448685055938	26.383105048504333	23.525127482308797
165-168	23.3800455305417	26.736162645545473	26.3155780301969	23.56821379371592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	2.0
13	6.0
14	13.0
15	27.0
16	59.0
17	106.5
18	193.0
19	394.0
20	776.5
21	1444.0
22	2705.5
23	4718.5
24	7746.0
25	12178.0
26	18238.5
27	26190.0
28	35756.5
29	47234.5
30	60192.0
31	74623.5
32	90412.5
33	106409.5
34	123660.5
35	141828.0
36	160258.5
37	179777.5
38	199208.0
39	218792.0
40	238586.5
41	256709.5
42	273794.5
43	288879.5
44	300562.5
45	308112.5
46	311658.0
47	311449.5
48	306953.0
49	297722.5
50	285406.5
51	270498.0
52	253339.0
53	236370.0
54	220283.5
55	205593.0
56	192609.0
57	181609.5
58	171678.5
59	162804.0
60	154949.0
61	146316.5
62	137049.5
63	126655.5
64	114532.5
65	101933.5
66	88571.5
67	74850.5
68	61563.5
69	49220.5
70	38204.5
71	28679.0
72	21009.0
73	15006.5
74	10252.5
75	6697.0
76	4277.0
77	2712.0
78	1658.0
79	995.0
80	584.5
81	296.0
82	148.0
83	79.0
84	44.5
85	25.5
86	9.5
87	4.0
88	3.5
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1182.0
92-93	2888.0
94-95	4567.0
96-97	5929.0
98-99	7020.0
100-101	7847.0
102-103	9283.0
104-105	10214.0
106-107	10996.0
108-109	11638.0
110-111	12074.0
112-113	12629.0
114-115	13371.0
116-117	13971.0
118-119	14719.0
120-121	15685.0
122-123	16310.0
124-125	17304.0
126-127	18297.0
128-129	62242.0
130-131	108761.0
132-133	111039.0
134-135	111349.0
136-137	91030.0
138-139	86235.0
140-141	90695.0
142-143	91443.0
144-145	83814.0
146-147	81939.0
148-149	83189.0
150-151	84547.0
152-153	87324.0
154-155	88422.0
156-157	90906.0
158-159	94895.0
160-161	95968.0
162-163	100030.0
164-165	108726.0
166-167	206023.0
168-169	5608481.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.9684197721016
#Duplication Level	Percentage of total
1	86.7953521535102
2	11.0594239471915
3	1.5512580040134933
4	0.3289579380432174
5	0.12095107503293065
6	0.05364322206528217
7	0.04256700216994162
8	0.019852464007889835
9	0.009463871719328632
>10	0.016337574959092053
>50	0.0
>100	0.002192747287116622
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2865075462673141E-5	1.2865075462673141E-5	0.0	0.0	0.0
2	1.2865075462673141E-5	2.5730150925346283E-5	2.5730150925346283E-5	0.0	0.0	0.0
3	1.2865075462673141E-5	2.5730150925346283E-5	2.5730150925346283E-5	2.5730150925346283E-5	0.0	0.0
4	2.5730150925346283E-5	5.1460301850692565E-5	5.1460301850692565E-5	3.859522638801943E-5	1.2865075462673141E-5	0.0
5	2.5730150925346283E-5	5.1460301850692565E-5	5.1460301850692565E-5	3.859522638801943E-5	1.2865075462673141E-5	0.0
6	2.5730150925346283E-5	5.1460301850692565E-5	5.1460301850692565E-5	5.1460301850692565E-5	3.859522638801943E-5	0.0
7	3.859522638801943E-5	6.432537731336571E-5	5.1460301850692565E-5	6.432537731336571E-5	3.859522638801943E-5	0.0
8	6.432537731336571E-5	7.719045277603885E-5	5.1460301850692565E-5	6.432537731336571E-5	3.859522638801943E-5	0.0
9	6.432537731336571E-5	7.719045277603885E-5	5.1460301850692565E-5	7.719045277603885E-5	3.859522638801943E-5	0.0
10-14	7.20444225909696E-5	1.1063964897898902E-4	1.0549361879391976E-4	9.520155842378125E-5	3.859522638801943E-5	0.0
15-19	9.005552823871199E-5	1.5180789045954308E-4	1.4151583008940456E-4	1.5952693573714695E-4	4.1168241480554055E-5	0.0
20-24	9.005552823871199E-5	2.1356025268037416E-4	2.187062828654434E-4	1.9554914703263175E-4	5.1460301850692565E-5	0.0
25-29	9.005552823871199E-5	2.4958246397585896E-4	2.7788562999373986E-4	2.161332677729088E-4	7.20444225909696E-5	0.0
30-34	1.4923487536700844E-4	2.650205545310667E-4	3.113348261966901E-4	2.418634186982551E-4	8.490949805364273E-5	0.0
35-39	3.1390784128922463E-4	3.0104276582655154E-4	3.6022211295484794E-4	2.7273959980867065E-4	1.2865075462673142E-4	0.0
40-44	4.940188977666487E-4	3.2419990165936317E-4	3.936713091577981E-4	3.1133482619668997E-4	1.543809055520777E-4	0.0
45-49	6.535458335037957E-4	3.5507608276977873E-4	4.2197447517567895E-4	3.3706497712203626E-4	1.8783010175502787E-4	1.0292060370138514E-5
50-54	7.847696032230615E-4	3.6279512804738255E-4	4.760077921189063E-4	3.6022211295484794E-4	2.4700944888332436E-4	3.344919620295017E-5
55-59	8.748251314617736E-4	3.80806233695125E-4	5.19749048691995E-4	3.8337924878765957E-4	3.061887960116207E-4	3.859522638801943E-5
60-64	9.314314634975354E-4	4.1425542989807524E-4	5.892204561904299E-4	4.116824148055405E-4	3.4993005258470946E-4	5.40333169432272E-5
65-69	0.0015541011158909156	4.657157317487677E-4	6.458267882261916E-4	4.5542367137862917E-4	3.8595226388019423E-4	7.20444225909696E-5
70-74	0.012679818376010649	4.940188977666486E-4	6.947140749843496E-4	4.91445882674114E-4	4.2197447517567906E-4	9.520155842378125E-5
75-79	0.017375570919886348	5.531982448949452E-4	7.178712108171614E-4	5.454791996173413E-4	4.6056970156369855E-4	1.2865075462673142E-4
80-84	0.017391009010441553	5.712093505426875E-4	7.384553315574382E-4	5.917934712829645E-4	5.557712599874797E-4	1.2865075462673142E-4
85-89	0.01740644710099676	5.892204561904299E-4	7.719045277603885E-4	6.561188485963302E-4	5.660633203576183E-4	1.2865075462673142E-4
90-94	0.017421885191551974	6.329617127635185E-4	7.821965881305269E-4	6.998601051694189E-4	5.660633203576183E-4	1.2865075462673142E-4
95-99	0.01745790740284745	6.586918636888649E-4	8.31083874888685E-4	7.410283466499727E-4	5.686363354501529E-4	1.2865075462673142E-4
100-104	0.017499075644328007	7.101521655395575E-4	8.799711616468431E-4	7.641854824827845E-4	5.789283958202914E-4	1.2865075462673142E-4
105-109	0.017550535946178704	7.796235730379924E-4	9.520155842378125E-4	7.950616635932E-4	5.789283958202914E-4	1.2865075462673142E-4
110-114	0.017581412127289114	8.079267390558732E-4	0.0010060489011810398	8.104997541484079E-4	5.789283958202914E-4	1.2865075462673142E-4
115-119	0.017643164489509946	8.23364829611081E-4	0.0011038234746973557	8.388029201662888E-4	6.046585467456377E-4	1.2865075462673142E-4
120-124	0.017787253334691885	8.336568899812196E-4	0.0011758678972883252	8.568140258140313E-4	6.818489995216765E-4	1.2865075462673142E-4
125-129	0.018003386602464793	8.490949805364273E-4	0.0012067440783987407	8.773981465543083E-4	7.513204070201115E-4	1.2865075462673142E-4
130-134	0.018268407156995858	8.748251314617736E-4	0.001235047244416622	9.082743276647237E-4	7.950616635932E-4	1.2865075462673142E-4
135-139	0.018422788062547938	9.082743276647237E-4	0.001253058350064364	9.185663880348624E-4	8.31083874888685E-4	1.3636979990433532E-4
140-144	0.018554011832267202	9.262854333124661E-4	0.0012813615160822446	9.468695540527433E-4	8.748251314617736E-4	1.4923487536700847E-4
145-149	0.018651786405783518	9.725997049780895E-4	0.0013096646821001256	9.803187502556935E-4	8.92836237109516E-4	1.6724598101475083E-4
150-154	0.018764999069855043	0.0010292060370138511	0.0013353948330254721	0.001018913976643713	9.237124182199316E-4	1.6724598101475083E-4
155-157	0.01892023764710463	0.0010549361879391976	0.0013379678481180064	0.0010549361879391976	9.605923012129279E-4	1.7582269798986625E-4
>>END_MODULE
