##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293535
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7803528
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.91993570087786	41.0	40.0	41.0	38.0	41.0
2	40.04995637870461	41.0	40.0	41.0	38.0	41.0
3	40.07489394540521	41.0	40.0	41.0	38.0	41.0
4	40.07299185701647	41.0	40.0	41.0	38.0	41.0
5	40.05431492012331	41.0	40.0	41.0	38.0	41.0
6	40.037350926401494	41.0	40.0	41.0	38.0	41.0
7	40.02676122902359	41.0	40.0	41.0	38.0	41.0
8	39.99725636917046	41.0	40.0	41.0	38.0	41.0
9	39.96328045468665	41.0	40.0	41.0	38.0	41.0
10-14	39.86440287008646	41.0	40.0	41.0	38.0	41.0
15-19	39.64289641813293	40.2	39.4	41.0	37.2	41.0
20-24	39.33299112914057	40.0	38.6	41.0	36.0	41.0
25-29	39.05880204440864	40.0	38.0	41.0	35.0	41.0
30-34	38.62805287557116	40.0	37.0	41.0	35.0	41.0
35-39	38.04502740299004	39.0	35.4	41.0	34.0	41.0
40-44	37.54949187085636	37.8	35.0	40.4	34.0	41.0
45-49	36.807700311961455	36.4	35.0	39.4	34.0	41.0
50-54	36.146807085205566	35.2	35.0	38.0	33.4	40.4
55-59	35.3225129966856	34.8	34.6	36.4	32.4	39.0
60-64	35.14802853273545	35.0	34.8	36.0	32.8	38.2
65-69	35.11773331241971	35.0	35.0	35.2	33.0	37.0
70-74	34.99596538898817	35.0	35.0	35.0	33.0	36.6
75-79	34.9468583697015	35.0	35.0	35.0	33.0	36.0
80-84	34.817229822203494	35.0	34.6	35.0	32.6	36.6
85-89	39.779338089130974	41.0	40.0	41.0	37.0	41.0
90-94	39.68204879001497	41.0	39.8	41.0	37.0	41.0
95-99	39.48107267118803	40.6	39.0	41.0	36.0	41.0
100-104	39.244973337069354	40.0	38.4	41.0	35.0	41.0
105-109	38.905591283095255	40.0	38.0	41.0	35.0	41.0
110-114	38.5483333295843	40.0	37.0	41.0	35.0	41.0
115-119	37.799005259301595	38.8	35.6	40.4	33.8	40.6
120-124	37.64085373963452	38.6	35.0	40.4	34.0	41.0
125-129	37.027895579000976	36.8	35.0	39.8	33.4	41.0
130-134	36.2996811963734	35.6	35.0	38.8	33.0	40.8
135-139	35.77967660687411	35.0	35.0	37.2	33.0	39.6
140-144	35.39431693551619	35.0	35.0	36.2	33.0	38.4
145-149	34.980435214962576	35.0	35.0	35.4	33.0	36.8
150-154	34.70723296216954	35.0	35.0	35.0	33.0	36.0
155-159	34.54617029576995	35.0	35.0	35.0	33.0	36.0
160-164	34.455764591870995	35.0	34.6	35.0	33.0	35.0
165-168	34.206919333572614	35.0	33.75	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	7.0
30	195.0
31	2427.0
32	16311.0
33	72774.0
34	248626.0
35	758842.0
36	1853810.0
37	2727910.0
38	1885176.0
39	236709.0
40	741.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.83346353085425	25.92549164941806	26.478933631044832	23.762111188682862
2	23.505419599955303	25.98719450997036	26.48824993003165	24.01913596004269
3	23.83592395644637	26.118084025584327	26.090916826338034	23.955075191631273
4	23.965288520781883	26.25491956971257	26.157335502608564	23.622456406896983
5	23.560061551646896	26.19426751592357	26.323362971209946	23.92230796121959
6	23.84008873934969	26.16604950991398	26.10744781078507	23.88641393995126
7	23.984869407785812	26.156143733962384	26.1065123364714	23.752474521780407
8	23.691566173658888	26.003187276319125	26.03878655910506	24.26645999091693
9	23.9935706003746	26.10351369278101	25.769228994885392	24.133686711959
10-14	23.70640817845467	26.24318897811349	26.214212340879662	23.836190502552178
15-19	23.732263150718495	25.9796697083678	26.27132496993668	24.016742170977025
20-24	23.689938704647435	26.074839482859545	26.093548969132936	24.14167284336008
25-29	23.728805740172906	26.072602033336718	26.180063684015742	24.018528542474634
30-34	23.672392794643653	26.15900654165654	26.12364048671319	24.044960176986617
35-39	23.723941273741826	26.048179746391632	26.120841752602157	24.107037227264385
40-44	23.829793396012676	26.04592691920885	26.044545492756612	24.079734192021863
45-49	24.000370089016148	26.021363670380882	25.963646186699147	24.014620053903823
50-54	23.94652008681202	25.99248442499341	25.921744626276734	24.13925086191784
55-59	23.91341454788142	26.037492272725878	26.029390808875164	24.01970237051754
60-64	23.911189913075216	26.070966875495287	25.951920720986717	24.065922490442784
65-69	23.85523573440116	26.03392465561731	26.013539004409285	24.09730060557225
70-74	23.85208587705458	26.06606909080098	26.004917263063575	24.076927769080857
75-79	23.84117030143289	26.099905068579236	25.98121772613618	24.077706903851695
80-84	23.820425838159355	26.057298698742414	25.930153643326452	24.192121819771774
85-89	23.63133187963188	26.200290432737606	26.251723579386145	23.916654108244373
90-94	23.79585338728684	26.22431496248533	26.009790884654443	23.970040765573383
95-99	23.590246064288547	26.272794275616306	26.23698363397201	23.89997602612313
100-104	23.6732459103303	26.0570732981531	26.240162338623236	24.029518452893363
105-109	23.688617840469238	26.23555364295183	26.044140324038263	24.031688192540667
110-114	23.684479263084853	26.134007542978427	26.082371867830307	24.099141326106412
115-119	23.587803125531863	26.246793328816143	26.180041058000175	23.98536248765182
120-124	23.678019313999396	26.21081887650167	26.08543268175793	24.025729127741002
125-129	23.73051762429635	26.195681411165182	25.97940707924894	24.094393885289527
130-134	23.886975365037454	26.18123985106502	25.98037928405822	23.951405499839304
135-139	23.78385331308055	26.32218015431686	25.984962230402235	23.90900430220035
140-144	23.71431011266241	26.329836426311676	26.130066003418094	23.82578745760782
145-149	23.60562562235318	26.44392242808627	26.231056773262335	23.71939517629822
150-154	23.58972371558158	26.540610012548242	26.2159121116056	23.65375416026458
155-159	23.482052908437545	26.58902162341593	26.258480644803804	23.67044482334272
160-164	23.408984842789696	26.684355177769937	26.41996913809541	23.486690841344952
165-168	23.356080993140534	26.78388942621907	26.327796084424104	23.532233496216296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	2.5
14	7.5
15	19.0
16	44.5
17	97.0
18	207.5
19	422.5
20	801.0
21	1537.0
22	2789.0
23	4797.5
24	7864.5
25	12309.0
26	18570.5
27	26667.5
28	36632.5
29	48205.5
30	61226.5
31	75595.5
32	90985.5
33	107448.0
34	124897.0
35	143132.0
36	162106.5
37	181592.5
38	201363.5
39	220969.0
40	240228.5
41	259036.0
42	275875.5
43	290370.5
44	302190.5
45	309984.5
46	313897.5
47	313121.5
48	307427.5
49	298220.5
50	286185.5
51	271400.5
52	254496.0
53	237184.5
54	220918.5
55	205733.5
56	192401.0
57	181432.0
58	171495.5
59	162691.0
60	154767.5
61	146349.0
62	136652.0
63	125742.0
64	114018.0
65	101383.5
66	87773.0
67	74175.5
68	61081.5
69	48861.5
70	38003.5
71	28611.5
72	20796.5
73	14656.0
74	10070.0
75	6594.0
76	4161.5
77	2556.0
78	1561.5
79	947.5
80	531.5
81	288.0
82	153.5
83	82.5
84	50.5
85	25.0
86	10.0
87	6.0
88	4.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1230.0
92-93	3062.0
94-95	4889.0
96-97	6422.0
98-99	7610.0
100-101	8541.0
102-103	9961.0
104-105	11678.0
106-107	11977.0
108-109	12435.0
110-111	13040.0
112-113	13726.0
114-115	14399.0
116-117	14951.0
118-119	15793.0
120-121	16681.0
122-123	17656.0
124-125	18508.0
126-127	19698.0
128-129	65212.0
130-131	112989.0
132-133	115438.0
134-135	118419.0
136-137	95053.0
138-139	90564.0
140-141	98386.0
142-143	95464.0
144-145	86788.0
146-147	84436.0
148-149	84901.0
150-151	87462.0
152-153	89191.0
154-155	92739.0
156-157	96736.0
158-159	99332.0
160-161	98521.0
162-163	103558.0
164-165	109210.0
166-167	214962.0
168-169	5541910.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.98990881328693
#Duplication Level	Percentage of total
1	86.81678579763523
2	11.049298081076502
3	1.5987703448479964
4	0.31264632265643655
5	0.10351808480200186
6	0.05260643714718406
7	0.029066621642776718
8	0.014626025599075627
9	0.008410929704302267
>10	0.010668076914021078
>50	0.0013553797062450666
>100	0.002247898268214925
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2814716625608314E-5	1.2814716625608314E-5	0.0	1.2814716625608314E-5	0.0	0.0
2	2.562943325121663E-5	3.844414987682495E-5	0.0	3.844414987682495E-5	0.0	0.0
3	2.562943325121663E-5	5.125886650243326E-5	0.0	6.407358312804158E-5	0.0	0.0
4	2.562943325121663E-5	5.125886650243326E-5	0.0	7.68882997536499E-5	0.0	0.0
5	2.562943325121663E-5	5.125886650243326E-5	1.2814716625608314E-5	7.68882997536499E-5	0.0	0.0
6	3.844414987682495E-5	6.407358312804158E-5	1.2814716625608314E-5	1.0251773300486652E-4	0.0	0.0
7	3.844414987682495E-5	7.68882997536499E-5	1.2814716625608314E-5	1.2814716625608315E-4	0.0	0.0
8	5.125886650243326E-5	8.97030163792582E-5	1.2814716625608314E-5	1.2814716625608315E-4	0.0	0.0
9	5.125886650243326E-5	1.0251773300486652E-4	2.562943325121663E-5	1.2814716625608315E-4	0.0	0.0
10-14	6.151063980291991E-5	1.230212796058398E-4	5.125886650243326E-5	1.383989395565698E-4	7.688829975364987E-6	0.0
15-19	7.68882997536499E-5	1.435248262068131E-4	1.102065629802315E-4	1.7684308943339476E-4	1.2814716625608314E-5	0.0
20-24	7.68882997536499E-5	1.794060327585164E-4	1.4096188288169147E-4	2.3066489926094964E-4	1.2814716625608314E-5	0.0
25-29	9.226595970437986E-5	2.0759840933485472E-4	1.5633954283242145E-4	2.767978791131396E-4	3.075531990145995E-5	0.0
30-34	1.4865071285705646E-4	2.306648992609497E-4	2.1528723931021969E-4	3.3574557559093785E-4	9.995478967974485E-5	0.0
35-39	3.4856029221654614E-4	2.3835372923631466E-4	2.767978791131396E-4	3.7931561211800615E-4	1.383989395565698E-4	0.0
40-44	5.459069282509143E-4	2.6910904913777465E-4	2.947384823889913E-4	4.2032270531995266E-4	1.460877695319348E-4	0.0
45-49	6.61239377881389E-4	3.0242731236435625E-4	3.2036791564020786E-4	4.664556851721426E-4	1.665913161329081E-4	5.1258866502433254E-6
50-54	7.176241310340656E-4	3.3061968894069446E-4	3.408714622411812E-4	5.279663249750625E-4	1.9734663603436804E-4	1.2814716625608314E-5
55-59	7.816977141621072E-4	3.5368617886678946E-4	3.998191587189794E-4	5.664104748518875E-4	2.332278425860713E-4	1.2814716625608314E-5
60-64	8.380824673147839E-4	3.588120655170328E-4	4.5876685519677764E-4	6.253581713296858E-4	3.101161423397212E-4	2.3066489926094965E-5
65-69	0.0014839441852454427	3.818785554431278E-4	5.151516083494542E-4	6.407358312804157E-4	3.870044420933711E-4	2.562943325121663E-5
70-74	0.01219192139760375	4.0494504536922274E-4	5.664104748518875E-4	6.638023212065106E-4	4.433891952460477E-4	3.844414987682495E-5
75-79	0.016884670625901516	4.25448591970196E-4	5.920399081031041E-4	7.07372357733579E-4	5.279663249750625E-4	7.176241310340657E-5
80-84	0.01692567771910346	4.767074584726293E-4	6.227952280045641E-4	7.432535642852823E-4	6.663652645316323E-4	7.68882997536499E-5
85-89	0.01694105537905419	4.920851184233593E-4	6.561134912311457E-4	7.714459408616205E-4	6.663652645316323E-4	7.68882997536499E-5
90-94	0.01694105537905419	5.125886650243326E-4	6.843058678074839E-4	8.047642040882021E-4	6.91994697782849E-4	7.68882997536499E-5
95-99	0.01696412186898029	5.279663249750626E-4	7.048094144084573E-4	8.457712972901488E-4	7.022464710833358E-4	7.68882997536499E-5
100-104	0.016976936585605895	5.638475315267658E-4	7.22750017684309E-4	8.919042771423387E-4	7.124982443838223E-4	7.68882997536499E-5
105-109	0.017005128962182234	6.099805113789559E-4	7.381276776350389E-4	9.35474313669407E-4	7.22750017684309E-4	7.68882997536499E-5
110-114	0.017020506622132964	6.176693413543208E-4	7.586312242360121E-4	9.739184635462319E-4	7.406906209601607E-4	7.68882997536499E-5
115-119	0.01716146850501465	6.279211146548074E-4	8.252677506891754E-4	0.0010354291033491518	7.688829975364989E-4	7.68882997536499E-5
120-124	0.017379318687649994	6.586764345562673E-4	8.81652503841852E-4	0.0011046285731274366	8.098900907384455E-4	8.97030163792582E-5
125-129	0.01758179121033461	6.894317544577273E-4	9.27785483694042E-4	0.0011302580063786533	8.867783904920953E-4	8.97030163792582E-5
130-134	0.017748382526467515	7.304388476596739E-4	9.585408035955019E-4	0.0011763909862308433	9.43163143644772E-4	8.97030163792582E-5
135-139	0.017873966749398475	7.355647343099172E-4	9.764814068713536E-4	0.0011892057028564517	9.995478967974485E-4	8.97030163792582E-5
140-144	0.017961106822452614	7.586312242360122E-4	0.0010277402733737868	0.0012327757393835199	0.00104824381997476	8.97030163792582E-5
145-149	0.018040558065531388	7.970753741128371E-4	0.0010405549899993954	0.00130966403913717	0.0010841250265264636	8.97030163792582E-5
150-154	0.018278911794767698	8.15015977388689E-4	0.001050806763299882	0.0013352934723883862	0.00112513211972841	8.97030163792582E-5
155-157	0.01845746351308451	8.457712972901489E-4	0.001059349907716954	0.0013583599623144813	0.0011874970739730371	8.97030163792582E-5
>>END_MODULE
