##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293538
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8839917
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.9760535081947	41.0	40.0	41.0	38.0	41.0
2	40.09766426539978	41.0	40.0	41.0	38.0	41.0
3	40.11703107619675	41.0	40.0	41.0	38.0	41.0
4	40.11676172977642	41.0	40.0	41.0	38.0	41.0
5	40.10246170863369	41.0	40.0	41.0	38.0	41.0
6	40.087825824608984	41.0	40.0	41.0	38.0	41.0
7	40.08550317836695	41.0	40.0	41.0	38.0	41.0
8	40.061757254055664	41.0	40.0	41.0	38.0	41.0
9	40.032586957547224	41.0	40.0	41.0	38.0	41.0
10-14	39.95391766687403	41.0	40.0	41.0	38.0	41.0
15-19	39.778302284964894	40.8	40.0	41.0	37.8	41.0
20-24	39.552929377051846	40.0	39.0	41.0	36.8	41.0
25-29	39.39474868372633	40.0	39.0	41.0	36.0	41.0
30-34	39.10534831944689	40.0	38.0	41.0	35.0	41.0
35-39	38.62658443512535	40.0	37.2	41.0	35.0	41.0
40-44	38.148869904547745	39.0	35.6	41.0	35.0	41.0
45-49	37.39284690116435	37.4	35.0	40.2	34.0	41.0
50-54	36.63902688226598	36.2	35.0	39.0	34.0	41.0
55-59	35.68544645837738	34.8	34.6	37.0	33.2	39.6
60-64	35.38113891793328	35.0	34.8	36.0	33.4	38.8
65-69	35.231452806627026	35.0	35.0	36.0	33.8	37.0
70-74	35.04084642423679	35.0	35.0	35.0	33.0	36.6
75-79	34.95726220053876	35.0	35.0	35.0	33.0	36.0
80-84	34.82714303765521	35.0	34.8	35.0	32.6	36.0
85-89	39.90446840168296	41.0	40.0	41.0	38.0	41.0
90-94	39.844737508723384	41.0	40.0	41.0	37.8	41.0
95-99	39.687334479855636	41.0	39.8	41.0	37.0	41.0
100-104	39.502229699955834	40.4	39.0	41.0	36.2	41.0
105-109	39.241929257218416	40.0	38.4	41.0	35.2	41.0
110-114	38.98627686719508	40.0	38.0	41.0	35.0	41.0
115-119	38.335535382475896	39.4	37.0	40.4	34.4	40.6
120-124	38.26383151131016	39.2	36.2	40.8	35.0	41.0
125-129	37.68308709378844	38.4	35.0	40.6	34.0	41.0
130-134	36.92394800447054	36.8	35.0	39.6	34.0	41.0
135-139	36.293291611746746	35.6	35.0	38.4	33.6	40.6
140-144	35.76397122331229	35.0	35.0	36.8	34.0	39.0
145-149	35.2417168965578	35.0	35.0	36.0	34.0	37.4
150-154	34.89022677645802	35.0	35.0	35.0	33.2	36.2
155-159	34.68900089886731	35.0	35.0	35.0	33.0	36.0
160-164	34.58251719869314	35.0	35.0	35.0	33.0	35.8
165-168	34.35137055908571	35.0	34.75	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	171.0
31	2288.0
32	15363.0
33	66985.0
34	212323.0
35	581841.0
36	1538504.0
37	3086956.0
38	2850529.0
39	483284.0
40	1667.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.365651170706695	27.48411551827919	27.901958807984283	22.248274503029837
2	22.271532639955783	27.634388422425232	27.770611420899087	22.323467516719898
3	22.319858885552886	27.714773792559367	27.56059813683771	22.404769185050043
4	22.561953918798107	27.797546062932494	27.49379886711606	22.14670115115334
5	22.3180828507779	27.85199227549309	27.606831602604416	22.223093271124604
6	22.220627184621755	27.75608639764378	27.723529530876817	22.299756886857647
7	22.513627673201004	27.641752744963554	27.627883836465884	22.216735745369558
8	22.326137225044082	27.61256695057205	27.527000536317253	22.53429528806662
9	22.28499430481078	27.627770713231808	27.55559808989157	22.531636892065844
10-14	22.359834374010525	27.671581079324614	27.637879405428805	22.330705141236056
15-19	22.3329879680997	27.516213104715803	27.651485868023425	22.499313059161075
20-24	22.37641824012601	27.489979826733666	27.48307478452569	22.65052714861463
25-29	22.380757647385153	27.585019180609955	27.536640898325178	22.49758227367972
30-34	22.274367508201717	27.54940798652295	27.604311216949206	22.571913288326122
35-39	22.338096613350555	27.60778862516469	27.51015422429871	22.543960537186038
40-44	22.432316955012137	27.48426484094817	27.508016195174683	22.575402008865016
45-49	22.530792992739638	27.552344665679552	27.40494735414371	22.5119149874371
50-54	22.49722706672472	27.451438740884104	27.423596850513416	22.627737341877758
55-59	22.509109531232024	27.5410436545954	27.412345613652256	22.53750120052032
60-64	22.426948126322905	27.486495631124136	27.49384185394501	22.59271438860795
65-69	22.430997938102813	27.54060473644719	27.42149728328897	22.60690004216103
70-74	22.40061529989478	27.505133815170435	27.50375371171471	22.590497173220065
75-79	22.387058611523162	27.54753692823134	27.437167113673127	22.628237346572373
80-84	22.34006269515879	27.51947105385718	27.459676374789492	22.680789876194538
85-89	22.309876891378053	27.769190593079095	27.63638617873901	22.28454633680384
90-94	22.311975860876775	27.735150569584565	27.602798762142893	22.35007480739576
95-99	22.2597666967633	27.741903744572284	27.622877721270676	22.375451837393737
100-104	22.357243311609366	27.535922601502932	27.617182128712802	22.489651958174896
105-109	22.384039787692988	27.599363276430562	27.43188789730415	22.5847090385723
110-114	22.311115987059104	27.633018761670254	27.519246050253948	22.5366192010167
115-119	22.312884885749934	27.628682495912855	27.551804943077595	22.50662767525962
120-124	22.30409206576185	27.67818680135908	27.516463505947826	22.50125762693125
125-129	22.370676116275703	27.59916044795935	27.44081857247523	22.589344863289725
130-134	22.503373411703226	27.645096254029266	27.39929986154634	22.452230472721173
135-139	22.432255626739543	27.63918511819959	27.42984960385837	22.498709651202496
140-144	22.390438861109725	27.744244054087062	27.462275277916405	22.403041806886804
145-149	22.337354807341082	27.713026783953655	27.58171641469807	22.367901994007198
150-154	22.306536859614365	27.860774414286883	27.540235780988574	22.29245294511017
155-159	22.22034264184378	27.806389000748606	27.625016417731235	22.348251939676377
160-164	22.2171261713656	27.93562754881061	27.627741246543792	22.219505033279997
165-168	22.105358391768274	27.946921681358504	27.667586557888114	22.280133368985105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.5
14	5.5
15	11.0
16	27.0
17	74.0
18	173.0
19	384.5
20	785.0
21	1612.0
22	3139.0
23	5658.0
24	9754.0
25	16106.0
26	25056.0
27	36722.0
28	51191.0
29	69111.0
30	89947.5
31	113283.5
32	139061.0
33	166946.0
34	196732.0
35	227209.5
36	258425.5
37	288879.5
38	318049.0
39	345468.5
40	369455.5
41	389175.5
42	401885.5
43	408800.0
44	410208.0
45	403929.5
46	391879.0
47	375149.0
48	353766.5
49	329421.0
50	303285.0
51	276268.5
52	249445.0
53	223622.5
54	199808.0
55	178631.5
56	159923.0
57	143132.5
58	128418.0
59	115509.5
60	103716.5
61	92631.5
62	82455.0
63	72805.5
64	63043.5
65	53713.0
66	44972.0
67	36856.5
68	29597.5
69	23273.0
70	17921.0
71	13560.0
72	9959.5
73	7046.0
74	4910.5
75	3268.5
76	2061.0
77	1345.5
78	861.5
79	502.5
80	309.0
81	181.5
82	99.5
83	48.0
84	20.5
85	10.0
86	6.5
87	3.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	974.0
92-93	2420.0
94-95	3830.0
96-97	5080.0
98-99	6061.0
100-101	7035.0
102-103	8185.0
104-105	10108.0
106-107	9817.0
108-109	10360.0
110-111	10904.0
112-113	11429.0
114-115	12054.0
116-117	12725.0
118-119	13604.0
120-121	14278.0
122-123	14886.0
124-125	15527.0
126-127	16463.0
128-129	57916.0
130-131	100592.0
132-133	102905.0
134-135	107279.0
136-137	87642.0
138-139	85527.0
140-141	94721.0
142-143	90516.0
144-145	81998.0
146-147	79667.0
148-149	80476.0
150-151	82823.0
152-153	84757.0
154-155	88951.0
156-157	91485.0
158-159	95144.0
160-161	94585.0
162-163	98661.0
164-165	105492.0
166-167	207299.0
168-169	6735741.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.815481321319
#Duplication Level	Percentage of total
1	90.1110988707259
2	8.655316629708079
3	0.9501006859960895
4	0.15653830921019335
5	0.05520102195129109
6	0.028784595499648596
7	0.016541166177100646
8	0.01039645998293389
9	0.007318434202350029
>10	0.008703826546415074
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.393697022268422E-5	0.0	0.0	0.0	0.0	0.0
2	5.656161703780703E-5	0.0	0.0	3.393697022268422E-5	0.0	0.0
3	5.656161703780703E-5	0.0	0.0	5.656161703780703E-5	0.0	0.0
4	5.656161703780703E-5	0.0	1.1312323407561407E-5	5.656161703780703E-5	1.1312323407561407E-5	0.0
5	5.656161703780703E-5	1.1312323407561407E-5	2.2624646815122813E-5	6.787394044536844E-5	2.2624646815122813E-5	0.0
6	6.787394044536844E-5	1.1312323407561407E-5	2.2624646815122813E-5	7.918626385292984E-5	2.2624646815122813E-5	0.0
7	7.918626385292984E-5	2.2624646815122813E-5	4.5249293630245627E-5	1.0181091066805265E-4	2.2624646815122813E-5	0.0
8	7.918626385292984E-5	2.2624646815122813E-5	6.787394044536844E-5	1.1312323407561406E-4	2.2624646815122813E-5	0.0
9	7.918626385292984E-5	3.393697022268422E-5	7.918626385292984E-5	1.2443555748317545E-4	2.2624646815122813E-5	0.0
10-14	8.371119321595441E-5	4.751175831175791E-5	9.502351662351582E-5	1.6515992175039653E-4	2.4887111496635096E-5	0.0
15-19	9.72859813050281E-5	5.656161703780703E-5	1.2896048684620003E-4	2.0814675069912988E-4	3.61994349041965E-5	0.0
20-24	1.0633584003107721E-4	5.656161703780703E-5	1.583725277058597E-4	2.5792097369240006E-4	4.5249293630245627E-5	0.0
25-29	1.3574788089073687E-4	8.59736578974667E-5	1.900470332470316E-4	3.1900752009323167E-4	4.977422299327018E-5	0.0
30-34	1.538475983428351E-4	1.1086076939410177E-4	2.036218213361053E-4	4.1176857203523517E-4	5.656161703780703E-5	0.0
35-39	2.036218213361053E-4	1.3122295152771232E-4	2.375587915587895E-4	4.6833018907304225E-4	7.466133448990528E-5	0.0
40-44	2.647083677369369E-4	1.8552210388400706E-4	2.5565850901088777E-4	5.294167354738738E-4	1.085983047125895E-4	0.0
45-49	3.280573788192808E-4	2.2172153878820353E-4	2.941204085965966E-4	5.543038469705088E-4	1.1764816343863861E-4	1.3574788089073688E-5
50-54	3.5520695499742814E-4	2.5792097369240006E-4	3.3031984350079306E-4	5.63353705696558E-4	1.3574788089073687E-4	2.4887111496635096E-5
55-59	3.77831601812551E-4	2.6018343837391235E-4	3.733066724495264E-4	6.244402520973896E-4	1.6968485111342108E-4	4.751175831175791E-5
60-64	4.2986828948733344E-4	2.6697083241844917E-4	4.1855596607977204E-4	7.036265159503195E-4	2.1040921538064214E-4	6.787394044536844E-5
65-69	6.832643338167089E-4	2.8054562050752285E-4	4.389181482133825E-4	7.375634861730037E-4	2.3529632687727727E-4	7.918626385292984E-5
70-74	0.005133532362351366	3.031702673226457E-4	4.6833018907304225E-4	7.669755270326633E-4	2.9185794391508425E-4	1.1086076939410177E-4
75-79	0.007000065724598997	3.4163216690835446E-4	4.977422299327018E-4	8.05437426618372E-4	3.9819378394616154E-4	1.3574788089073687E-4
80-84	0.0070226903714141215	3.710442077680141E-4	5.045296239772387E-4	8.868861551528141E-4	5.63353705696558E-4	1.3574788089073687E-4
85-89	0.0070362651595031935	3.733066724495264E-4	5.135794827032878E-4	9.343979134645722E-4	5.656161703780703E-4	1.3574788089073687E-4
90-94	0.007054364876955294	3.9593131926464925E-4	5.339416648368983E-4	9.954844598654036E-4	5.656161703780703E-4	1.3574788089073687E-4
95-99	0.0070769895237704165	4.321307541688457E-4	5.452539882444598E-4	0.0010248965007250634	5.656161703780703E-4	1.3574788089073687E-4
100-104	0.00710187663526705	4.4570554225791936E-4	5.882408171931932E-4	0.0010497836122216983	5.656161703780703E-4	1.3574788089073687E-4
105-109	0.007122238817400661	4.75117583117579E-4	6.244402520973896E-4	0.0010746707237183335	5.701410997410949E-4	1.3574788089073687E-4
110-114	0.007156175787623345	5.113170180217755E-4	6.380150401864633E-4	0.0010882455118074073	5.79190958467144E-4	1.3574788089073687E-4
115-119	0.0072082124752981275	5.294167354738738E-4	6.696895457276353E-4	0.0011176575526670668	5.972906759192422E-4	1.4253527493527372E-4
120-124	0.007334910497462816	5.429915235629475E-4	6.832643338167089E-4	0.0011493320582082389	6.19915322734365E-4	1.4706020429829827E-4
125-129	0.007391472114500623	5.497789176074843E-4	7.013640512688071E-4	0.0012149435339720949	6.35752575504951E-4	1.5158513366132282E-4
130-134	0.007436721408130867	5.678786350595826E-4	7.149388393578809E-4	0.0012601928276023406	6.448024342310001E-4	1.674223864319088E-4
135-139	0.007497807954531699	5.927657465562178E-4	7.2398869808393E-4	0.0012896048684620002	6.448024342310001E-4	1.8325963920249477E-4
140-144	0.00751817013666531	6.086029993268036E-4	7.330385568099791E-4	0.0013280667680477091	6.51589828275537E-4	2.036218213361053E-4
145-149	0.007615456117970339	6.221777874158773E-4	7.375634861730037E-4	0.0013687911323149301	6.76476939772172E-4	2.036218213361053E-4
150-154	0.007669755270326632	6.289651814604142E-4	7.782878504402248E-4	0.0014027281025376145	6.900517278612458E-4	2.036218213361053E-4
155-157	0.007745170759710376	6.372608852926258E-4	8.031749619368598E-4	0.0014328942982911113	6.900517278612458E-4	2.036218213361053E-4
>>END_MODULE
