##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293539
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8785426
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.99953047239826	41.0	40.0	41.0	38.0	41.0
2	40.11335147550045	41.0	40.0	41.0	38.0	41.0
3	40.13003148623641	41.0	40.0	41.0	38.0	41.0
4	40.12868323061397	41.0	40.0	41.0	38.0	41.0
5	40.119558914957565	41.0	40.0	41.0	38.0	41.0
6	40.10088628599228	41.0	40.0	41.0	38.0	41.0
7	40.09775883377767	41.0	40.0	41.0	38.0	41.0
8	40.076780568181896	41.0	40.0	41.0	38.0	41.0
9	40.042871683171654	41.0	40.0	41.0	38.0	41.0
10-14	39.96845596331925	41.0	40.0	41.0	38.0	41.0
15-19	39.80005871086957	40.8	40.0	41.0	37.8	41.0
20-24	39.57391586930446	40.0	39.2	41.0	36.8	41.0
25-29	39.41502281164283	40.0	39.0	41.0	36.0	41.0
30-34	39.12317194408103	40.0	38.2	41.0	35.0	41.0
35-39	38.639268147042614	40.0	37.2	41.0	35.0	41.0
40-44	38.14448173600233	39.0	35.6	41.0	35.0	41.0
45-49	37.37191293854163	37.4	35.0	40.0	34.0	41.0
50-54	36.60881758038825	36.2	35.0	39.0	34.0	40.8
55-59	35.661300226078964	34.8	34.6	37.0	33.4	39.4
60-64	35.35773734819461	35.0	34.8	36.0	33.6	38.8
65-69	35.20955029386168	35.0	35.0	35.8	34.0	37.0
70-74	35.028890004878534	35.0	35.0	35.0	33.2	36.4
75-79	34.94466640547652	35.0	35.0	35.0	33.0	36.0
80-84	34.8199658161141	35.0	34.8	35.0	32.6	36.0
85-89	39.93142126517257	41.0	40.0	41.0	38.0	41.0
90-94	39.87586412430515	41.0	40.0	41.0	38.0	41.0
95-99	39.722287659711476	41.0	40.0	41.0	37.0	41.0
100-104	39.5395208095597	40.6	39.0	41.0	36.4	41.0
105-109	39.275377221926554	40.0	38.6	41.0	35.4	41.0
110-114	39.023334073593375	40.0	38.0	41.0	35.0	41.0
115-119	38.38170585508344	39.4	37.0	40.4	34.4	40.6
120-124	38.32084679899651	39.4	36.4	40.8	35.0	41.0
125-129	37.74708023148756	38.6	35.0	40.8	34.0	41.0
130-134	36.999216105773655	36.8	35.0	39.6	34.0	41.0
135-139	36.36535615061076	35.8	35.0	38.6	33.8	40.8
140-144	35.82937419330504	35.0	35.0	36.8	34.0	39.2
145-149	35.29359078126024	35.0	35.0	36.0	34.0	37.4
150-154	34.92750783335015	35.0	35.0	35.2	33.6	36.4
155-159	34.71812861558202	35.0	35.0	35.0	33.0	36.0
160-164	34.603099207192045	35.0	35.0	35.0	33.0	36.0
165-168	34.37252855646693	35.0	34.75	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	4.0
30	174.0
31	2235.0
32	14982.0
33	64398.0
34	204191.0
35	560298.0
36	1502685.0
37	3070477.0
38	2868810.0
39	495596.0
40	1576.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.3771505217846	27.493385067497012	27.885272723257813	22.244191687460575
2	22.265977768181074	27.602349618561465	27.764060615842645	22.36761199741481
3	22.328194443843703	27.724791034606632	27.561805198746196	22.38520932280347
4	22.54233317769679	27.780918079555843	27.51028806115947	22.166460681587893
5	22.32551955932473	27.823044665107872	27.596248605360742	22.255187170206657
6	22.25440177858194	27.765494809244313	27.68070666123646	22.29939675093729
7	22.48279138655314	27.682960393724787	27.588599573885205	22.245648645836866
8	22.34169407379904	27.60092680764712	27.507226172071793	22.55015294648205
9	22.34315103217533	27.604876530745347	27.50748796928003	22.544484467799286
10-14	22.384758576305806	27.66841129843903	27.607804106482714	22.339026018772454
15-19	22.358767804771222	27.489656164652686	27.63628081324685	22.515295217329246
20-24	22.39857919240342	27.47051537398414	27.462401937026158	22.66850349658628
25-29	22.396564492148702	27.561613972959307	27.53203316492564	22.50978836996635
30-34	22.29406974687397	27.541858527975766	27.58359810895909	22.58047361619118
35-39	22.354722468779546	27.580670533221724	27.499499739682516	22.565107258316218
40-44	22.451771832122883	27.46743982591169	27.496726965772634	22.5840613761928
45-49	22.551659987802527	27.522064382535348	27.395097289533826	22.5311783401283
50-54	22.518514184741868	27.435441377572356	27.395577630498508	22.650466807187268
55-59	22.51786993595985	27.52725934974582	27.409639555327196	22.545231158967134
60-64	22.44487404481012	27.482692358913503	27.469479567638494	22.602954028637885
65-69	22.44939061577663	27.525556529643524	27.406486606341	22.618566248238846
70-74	22.4302794195751	27.46605116245928	27.49377434856318	22.609895069402437
75-79	22.414425891243063	27.515580917760847	27.424580208176586	22.6454129828195
80-84	22.37185994168069	27.50550286349233	27.431862723560585	22.69077447126639
85-89	22.325681190644598	27.749459161115237	27.62216652897651	22.302693119263655
90-94	22.336424206316565	27.733917153681364	27.57656124657244	22.353097393429636
95-99	22.284106534258814	27.73053876208744	27.614964778706074	22.37038992494767
100-104	22.378125222561682	27.506439451352353	27.597657783678265	22.517777542407703
105-109	22.40814273373631	27.582385964856083	27.40706335544448	22.602407945963133
110-114	22.313705615017938	27.61560203499617	27.508318724407037	22.56237362557886
115-119	22.32026228495055	27.617105093483023	27.54319542340126	22.519437198165157
120-124	22.31031033880169	27.67473509415717	27.50840047910062	22.506554087940525
125-129	22.42139669269503	27.55855790291547	27.418849055309423	22.601196349080073
130-134	22.51729550717454	27.64252114827655	27.391556951376867	22.44862639317204
135-139	22.45292427793636	27.613522904487876	27.41426525977875	22.51928755779701
140-144	22.40591174948579	27.74280103712311	27.446608517776394	22.40467869561471
145-149	22.348877708279623	27.684390014955557	27.57169385786481	22.39503841890001
150-154	22.32486732166857	27.851697773227695	27.50752371726896	22.315911187834768
155-159	22.23942385613194	27.790987236417052	27.607518548038275	22.362070359412737
160-164	22.243079472734994	27.915545683070004	27.61070127916774	22.230673565027264
165-168	22.147758845732167	27.916327421970834	27.65190866109611	22.28400507120089
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	2.0
14	3.5
15	11.0
16	34.5
17	86.0
18	185.5
19	374.5
20	801.5
21	1643.5
22	3128.5
23	5603.5
24	9778.5
25	15994.5
26	24493.0
27	36053.0
28	50450.5
29	67709.5
30	88253.5
31	111903.5
32	137330.0
33	165049.5
34	194387.0
35	224283.0
36	255505.0
37	286279.5
38	315631.0
39	342373.5
40	366534.5
41	386055.0
42	399485.0
43	406912.0
44	407587.5
45	402093.5
46	390351.0
47	373127.0
48	351588.5
49	327686.5
50	301874.5
51	274828.0
52	248508.5
53	223208.5
54	199183.5
55	178261.0
56	159500.5
57	142555.5
58	128474.5
59	115445.0
60	103553.5
61	93109.0
62	82690.0
63	72446.5
64	62754.0
65	53563.5
66	45001.5
67	36981.5
68	29764.0
69	23346.5
70	18058.5
71	13596.0
72	9771.5
73	6986.0
74	4899.0
75	3348.0
76	2194.0
77	1395.0
78	877.5
79	521.5
80	309.0
81	168.5
82	86.5
83	45.5
84	25.0
85	15.5
86	10.0
87	3.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	937.0
92-93	2249.0
94-95	3512.0
96-97	4790.0
98-99	5601.0
100-101	6499.0
102-103	7453.0
104-105	8528.0
106-107	9274.0
108-109	9713.0
110-111	10493.0
112-113	10669.0
114-115	11228.0
116-117	11930.0
118-119	12493.0
120-121	13000.0
122-123	13644.0
124-125	14560.0
126-127	15108.0
128-129	55659.0
130-131	96124.0
132-133	98611.0
134-135	99240.0
136-137	84035.0
138-139	80631.0
140-141	86644.0
142-143	86143.0
144-145	78735.0
146-147	76902.0
148-149	77785.0
150-151	79751.0
152-153	82575.0
154-155	83733.0
156-157	85174.0
158-159	89680.0
160-161	90848.0
162-163	94988.0
164-165	106328.0
166-167	198629.0
168-169	6781530.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.93086996568076
#Duplication Level	Percentage of total
1	90.32960649877873
2	8.490844880782298
3	0.8824476551667293
4	0.1646882731012465
5	0.04600747191251247
6	0.028789712355840342
7	0.01447658126906871
8	0.013518710147016605
9	0.009412036131468467
>10	0.020208180355055498
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	3.4147461944361036E-5	0.0	0.0
2	0.0	0.0	2.2764974629574025E-5	4.552994925914805E-5	0.0	0.0
3	1.1382487314787012E-5	1.1382487314787012E-5	2.2764974629574025E-5	7.967741120350909E-5	0.0	0.0
4	2.2764974629574025E-5	1.1382487314787012E-5	2.2764974629574025E-5	7.967741120350909E-5	0.0	0.0
5	3.4147461944361036E-5	1.1382487314787012E-5	2.2764974629574025E-5	9.10598985182961E-5	0.0	0.0
6	5.691243657393506E-5	2.2764974629574025E-5	2.2764974629574025E-5	1.1382487314787011E-4	0.0	0.0
7	5.691243657393506E-5	2.2764974629574025E-5	3.4147461944361036E-5	1.1382487314787011E-4	0.0	0.0
8	5.691243657393506E-5	3.4147461944361036E-5	4.552994925914805E-5	1.3658984777744414E-4	0.0	0.0
9	5.691243657393506E-5	4.552994925914805E-5	4.552994925914805E-5	1.4797233509223116E-4	0.0	0.0
10-14	7.057142135167947E-5	6.601842642576467E-5	7.740091374055167E-5	1.6390781733293297E-4	0.0	0.0
15-19	8.87834010553387E-5	7.967741120350909E-5	1.1837786807378493E-4	1.889492894254644E-4	0.0	0.0
20-24	1.115483756849127E-4	9.10598985182961E-5	1.4114284270335894E-4	2.230967513698254E-4	0.0	0.0
25-29	1.2293086299969971E-4	9.56128934442109E-5	1.8211979703659223E-4	2.731796955548883E-4	2.048847716661662E-5	0.0
30-34	1.5707832494406078E-4	1.115483756849127E-4	2.139907615179958E-4	3.2098614227699373E-4	2.5041472092531425E-5	0.0
35-39	2.3675573614756986E-4	1.3658984777744414E-4	2.6862670062897346E-4	3.8245157377684365E-4	3.4147461944361036E-5	0.0
40-44	3.437511169065678E-4	1.5935482240701817E-4	3.141566498881215E-4	4.279815230359917E-4	5.235944164802025E-5	0.0
45-49	3.983870560175454E-4	1.775668021106774E-4	3.710690864620566E-4	5.008294418506285E-4	8.65069035923813E-5	0.0
50-54	4.575759900544379E-4	2.2082025390686801E-4	3.9383406109163064E-4	5.71400863202308E-4	1.2293086299969971E-4	6.829492388872207E-6
55-59	4.985529443876711E-4	2.504147209253143E-4	4.1887553318416205E-4	6.670137566465188E-4	1.5480182748110334E-4	2.5041472092531425E-5
60-64	5.190414215542878E-4	2.9139167525854754E-4	4.325345179619065E-4	6.943317262020078E-4	1.5935482240701817E-4	3.642395940731844E-5
65-69	8.036036044239629E-4	3.2781563466586594E-4	4.484700002026082E-4	7.307556856093262E-4	1.6618431479589038E-4	5.691243657393506E-5
70-74	0.005552377312153104	3.642395940731844E-4	4.735114722951397E-4	7.853916247203038E-4	1.7073730972180518E-4	7.512441627759427E-5
75-79	0.007724155891814466	3.892810661657158E-4	4.939999494617562E-4	8.172625892017074E-4	2.982211676474197E-4	1.2065436553674231E-4
80-84	0.007817492287795718	4.029400509434602E-4	5.463593911097766E-4	8.810045181645148E-4	5.440828936468192E-4	1.2520736046265713E-4
85-89	0.007853916247203038	4.1659903572120467E-4	5.873363454430099E-4	9.219814724977481E-4	5.577418784245636E-4	1.2520736046265713E-4
90-94	0.007881234216758525	4.325345179619065E-4	6.032718276837117E-4	9.9027639638647E-4	5.577418784245636E-4	1.2520736046265713E-4
95-99	0.007944976145721334	4.439170052766935E-4	6.16930812461456E-4	0.0010380828431085756	5.577418784245636E-4	1.2520736046265713E-4
100-104	0.007979123607665695	4.598524875173953E-4	6.806727414242632E-4	0.0010836127923677236	5.577418784245636E-4	1.2520736046265713E-4
105-109	0.007990506094980483	4.757879697580971E-4	7.216496957574965E-4	0.00110410126953434	5.577418784245636E-4	1.2520736046265713E-4
110-114	0.007990506094980483	4.917234519987989E-4	7.512441627759428E-4	0.0011405252289416587	5.577418784245636E-4	1.2520736046265713E-4
115-119	0.008038312541702588	5.008294418506285E-4	7.603501526277723E-4	0.0011837786807378493	5.577418784245636E-4	1.3431335031448676E-4
120-124	0.008115713455443138	5.14488426628373E-4	7.740091374055169E-4	0.0012475206097006563	5.691243657393505E-4	1.707373097218052E-4
125-129	0.008188561374257778	5.304239088690748E-4	7.876681221832613E-4	0.0012793915741820604	5.987188327577968E-4	1.8894928942546439E-4
130-134	0.008256856298146499	5.622948733504784E-4	8.081565993498779E-4	0.0013021565488116342	6.305897972392004E-4	1.935022843513792E-4
135-139	0.00829100376009086	5.987188327577968E-4	8.195390866646649E-4	0.0013476864980707822	6.465252794799023E-4	1.935022843513792E-4
140-144	0.008316045232183392	6.328662947021578E-4	8.400275638312815E-4	0.0014068754321076747	6.601842642576467E-4	1.935022843513792E-4
145-149	0.008377510663683242	6.374192896280727E-4	8.71898528312685E-4	0.0014432993915149933	6.647372591835615E-4	1.935022843513792E-4
150-154	0.008457188074886749	6.579077667946892E-4	8.923870054793018E-4	0.0014865528433111838	6.761197464983485E-4	2.003317767402514E-4
155-157	0.008533071323651997	6.601842642576468E-4	9.030106603064364E-4	0.001517664975304935	7.019200510785324E-4	2.2006142141921554E-4
>>END_MODULE
