##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293540
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8852482
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.980601824437485	41.0	40.0	41.0	38.0	41.0
2	40.10546036693438	41.0	40.0	41.0	38.0	41.0
3	40.12733174718684	41.0	40.0	41.0	38.0	41.0
4	40.125262384040994	41.0	40.0	41.0	38.0	41.0
5	40.111465349491816	41.0	40.0	41.0	38.0	41.0
6	40.09874563992336	41.0	40.0	41.0	38.0	41.0
7	40.09130919441576	41.0	40.0	41.0	38.0	41.0
8	40.07079347916211	41.0	40.0	41.0	38.0	41.0
9	40.040698303594404	41.0	40.0	41.0	38.0	41.0
10-14	39.960961253578375	41.0	40.0	41.0	38.0	41.0
15-19	39.79009434868097	40.8	40.0	41.0	37.8	41.0
20-24	39.567694325726954	40.0	39.0	41.0	36.8	41.0
25-29	39.41607775084999	40.0	39.0	41.0	36.0	41.0
30-34	39.12774015242279	40.0	38.2	41.0	35.0	41.0
35-39	38.656830073193035	40.0	37.2	41.0	35.0	41.0
40-44	38.174662428006066	39.0	35.6	41.0	35.0	41.0
45-49	37.41921463381682	37.4	35.0	40.2	34.0	41.0
50-54	36.66920454625042	36.2	35.0	39.0	34.0	41.0
55-59	35.69888996103013	35.0	34.6	37.0	33.4	39.6
60-64	35.399384782708395	35.0	34.8	36.0	33.6	38.8
65-69	35.24925143027684	35.0	35.0	36.0	34.0	37.0
70-74	35.05951463103794	35.0	35.0	35.0	33.2	36.6
75-79	34.97429276896581	35.0	35.0	35.0	33.0	36.0
80-84	34.84533763525303	35.0	34.8	35.0	32.6	36.0
85-89	39.910830454103156	41.0	40.0	41.0	38.0	41.0
90-94	39.85338130706468	41.0	40.0	41.0	37.8	41.0
95-99	39.70050774085169	41.0	39.8	41.0	37.0	41.0
100-104	39.51454875117529	40.6	39.0	41.0	36.2	41.0
105-109	39.25424931202107	40.0	38.4	41.0	35.2	41.0
110-114	39.00093361695294	40.0	38.0	41.0	35.0	41.0
115-119	38.33638724240699	39.4	36.8	40.4	34.4	40.6
120-124	38.285709169213774	39.4	36.2	40.8	35.0	41.0
125-129	37.720768130219824	38.4	35.0	40.8	34.0	41.0
130-134	36.95738269768183	36.8	35.0	39.6	34.0	41.0
135-139	36.32433986082488	35.8	35.0	38.6	33.8	40.6
140-144	35.79257905021556	35.0	35.0	36.8	34.0	39.0
145-149	35.263640522626076	35.0	35.0	36.0	34.0	37.4
150-154	34.910900192362774	35.0	35.0	35.2	33.6	36.4
155-159	34.70736373221262	35.0	35.0	35.0	33.0	36.0
160-164	34.59814813551967	35.0	35.0	35.0	33.0	36.0
165-168	34.36285425716156	35.0	34.75	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	3.0
30	177.0
31	2265.0
32	14956.0
33	65170.0
34	207553.0
35	570933.0
36	1512552.0
37	3074484.0
38	2891093.0
39	510964.0
40	2331.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.33709145073664	27.47440774237101	27.946467442690082	22.242033364202264
2	22.23672411872738	27.624907907183545	27.791697288963707	22.346670685125368
3	22.297159147005328	27.749867212381794	27.589019667026715	22.363953973586163
4	22.50775545208677	27.820355918261114	27.52644964429185	22.145438985360265
5	22.280248635354468	27.85334101780721	27.618943478224523	22.247466868613795
6	22.231313206849784	27.782671571656405	27.72231561724723	22.263699604246582
7	22.469653143604244	27.694888281049312	27.616537373360373	22.218921201986063
8	22.319345015330164	27.63727731951333	27.52617853388462	22.517199131271884
9	22.281434743386093	27.632216591911735	27.56957879157506	22.516769873127107
10-14	22.342945176279375	27.685713453018035	27.657269452793017	22.314071917909576
15-19	22.327082958203135	27.520806029314716	27.661760848539423	22.490350163942722
20-24	22.36428156532823	27.495197392098625	27.500411748930976	22.640109293642166
25-29	22.376813643902356	27.598748012139417	27.546071260014987	22.478367083943237
30-34	22.261345462210485	27.56893716361129	27.62041425218374	22.549303121994487
35-39	22.32342070845216	27.609389095623126	27.534535512187432	22.532654683737285
40-44	22.42758132690922	27.485575231895414	27.524633204563422	22.56221023663194
45-49	22.523545374054418	27.558034006733926	27.42146665759953	22.49695396161212
50-54	22.482108407562986	27.476350700289476	27.427964270359436	22.613576621788102
55-59	22.494891263263796	27.570744566326145	27.432182296445223	22.502181873964837
60-64	22.41130566546196	27.50894946750527	27.500773229473946	22.578971637558823
65-69	22.41510572966994	27.566863168995994	27.432717739499495	22.58531336183457
70-74	22.39701588774764	27.516163263590933	27.518262109993557	22.568558738667868
75-79	22.37969193272576	27.560871628996253	27.460822851715484	22.598613586562504
80-84	22.333088053723237	27.52588257169006	27.483230126872893	22.657799247713804
85-89	22.28449151322759	27.788068928013637	27.644766744513007	22.282672814245768
90-94	22.28853042914007	27.753709559478736	27.618119501059496	22.339640510321697
95-99	22.25779143357239	27.752533798340462	27.644814374496097	22.344860393591055
100-104	22.338593582712708	27.538467890765666	27.637924159331995	22.485014367189635
105-109	22.36923623047435	27.61995575735561	27.440359175895544	22.570448836274497
110-114	22.288864415327954	27.645835872323488	27.528139151214493	22.53716056113407
115-119	22.284534796877377	27.64809006047779	27.56443878461234	22.502936358032493
120-124	22.28980998940669	27.697621563057588	27.527682378733537	22.484886068802183
125-129	22.371876224953308	27.59958495699693	27.459858424220062	22.568680393829695
130-134	22.499257601345825	27.65521449004071	27.411442815964328	22.434085092649138
135-139	22.416594931924063	27.650698376341275	27.44146267960707	22.49124401212759
140-144	22.3647592542699	27.769391413684247	27.483569568572978	22.382279763472877
145-149	22.307899271825022	27.723983604028817	27.616833380070304	22.351283744075857
150-154	22.287535969822187	27.86538708475511	27.552333854680867	22.294743090741836
155-159	22.207448443674878	27.824673961648667	27.640335773931934	22.32754182074452
160-164	22.203687991505063	27.956176624297807	27.652244639725293	22.18789074447184
165-168	22.0948719153063	27.986188511299787	27.661362409000308	22.25757716439361
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.5
13	2.0
14	6.5
15	17.0
16	30.5
17	67.5
18	158.5
19	367.5
20	817.0
21	1627.5
22	3138.0
23	5776.5
24	9912.5
25	16171.0
26	24920.0
27	36682.5
28	51770.5
29	69597.5
30	90367.5
31	113875.0
32	139771.0
33	167447.0
34	197215.5
35	228245.0
36	259418.0
37	290181.0
38	319424.5
39	346700.0
40	370646.0
41	390233.0
42	403573.0
43	410534.5
44	410678.5
45	404968.5
46	393422.5
47	375470.0
48	354639.0
49	330995.0
50	303863.0
51	275670.5
52	248738.0
53	223461.5
54	200096.5
55	178582.5
56	159308.5
57	142749.5
58	127919.0
59	114462.0
60	102449.5
61	92021.5
62	82079.5
63	71993.5
64	62227.5
65	53172.5
66	44746.5
67	36834.0
68	29541.0
69	23182.0
70	17772.5
71	13268.0
72	9841.0
73	6994.0
74	4824.5
75	3189.5
76	2072.0
77	1374.5
78	821.0
79	478.5
80	285.5
81	156.0
82	91.5
83	55.0
84	27.5
85	14.5
86	5.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1016.0
92-93	2487.0
94-95	3846.0
96-97	5140.0
98-99	6479.0
100-101	7628.0
102-103	9067.0
104-105	9012.0
106-107	9924.0
108-109	10464.0
110-111	10880.0
112-113	11261.0
114-115	12209.0
116-117	12814.0
118-119	13586.0
120-121	14270.0
122-123	14665.0
124-125	15506.0
126-127	16290.0
128-129	57529.0
130-131	109969.0
132-133	105741.0
134-135	103187.0
136-137	89587.0
138-139	85392.0
140-141	90498.0
142-143	90384.0
144-145	82111.0
146-147	80836.0
148-149	86511.0
150-151	83295.0
152-153	83941.0
154-155	86845.0
156-157	91654.0
158-159	92366.0
160-161	94290.0
162-163	98815.0
164-165	103952.0
166-167	233777.0
168-169	6715258.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.98866481166945
#Duplication Level	Percentage of total
1	90.4428353735848
2	8.374009274344273
3	0.8985549452215368
4	0.14738894074483286
5	0.060311237729315584
6	0.029811846648791385
7	0.014473010741948783
8	0.010395996733144824
9	0.0062726056986770725
>10	0.015946768552662954
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.1296266967840206E-5	1.1296266967840206E-5	1.1296266967840206E-5	0.0	0.0
2	0.0	2.259253393568041E-5	1.1296266967840206E-5	1.1296266967840206E-5	0.0	0.0
3	0.0	2.259253393568041E-5	1.1296266967840206E-5	2.259253393568041E-5	0.0	0.0
4	0.0	2.259253393568041E-5	1.1296266967840206E-5	4.518506787136082E-5	0.0	0.0
5	0.0	3.388880090352062E-5	1.1296266967840206E-5	7.907386877488144E-5	0.0	0.0
6	1.1296266967840206E-5	3.388880090352062E-5	3.388880090352062E-5	7.907386877488144E-5	0.0	0.0
7	1.1296266967840206E-5	3.388880090352062E-5	4.518506787136082E-5	1.0166640271056185E-4	0.0	0.0
8	1.1296266967840206E-5	3.388880090352062E-5	5.648133483920103E-5	1.1296266967840205E-4	0.0	0.0
9	2.259253393568041E-5	3.388880090352062E-5	5.648133483920103E-5	1.4685147058192267E-4	0.0	0.0
10-14	3.1629547509952575E-5	6.55183484134732E-5	6.777760180704124E-5	1.8977728505971544E-4	0.0	0.0
15-19	1.0166640271056185E-4	1.0392565610412988E-4	9.94071493169938E-5	2.77888167408869E-4	0.0	0.0
20-24	1.3555520361408245E-4	1.3329595022051443E-4	1.4007371040121856E-4	3.2081398188666185E-4	6.777760180704124E-6	0.0
25-29	1.491107239754907E-4	1.5814773754976288E-4	1.6944400451760307E-4	3.4340651582234224E-4	2.937029411638454E-5	0.0
30-34	1.9429579184685155E-4	1.965550452404196E-4	1.807402714854433E-4	3.7503606333229484E-4	5.196282805206494E-5	6.777760180704124E-6
35-39	3.027399547381175E-4	2.3948085971821237E-4	2.0559205881469177E-4	4.405544117457681E-4	5.8740588232769064E-5	1.1296266967840206E-5
40-44	3.6825830315159074E-4	2.485178732924845E-4	2.3044384614394022E-4	5.264060407013536E-4	7.229610859417731E-5	1.1296266967840206E-5
45-49	4.1344337102295156E-4	2.711104072281649E-4	2.485178732924845E-4	5.783688687534185E-4	9.262938913628968E-5	1.8074027148544328E-5
50-54	4.586284388943124E-4	2.9144368777027726E-4	2.6433264704746085E-4	6.393687103797556E-4	1.2199968325267423E-4	2.259253393568041E-5
55-59	4.7896171943642474E-4	3.049992081316856E-4	2.9596219455741335E-4	7.003685520060928E-4	1.807402714854433E-4	2.259253393568041E-5
60-64	5.173690271270815E-4	3.0951771491882166E-4	3.2081398188666185E-4	7.297388461224773E-4	2.4174011311178036E-4	2.259253393568041E-5
65-69	7.839609275681102E-4	3.569620361837505E-4	3.2759174206736596E-4	7.613683936324299E-4	2.552956334731887E-4	2.259253393568041E-5
70-74	0.005264060407013536	3.931100904808392E-4	3.501842760030464E-4	8.133312216844948E-4	2.846659275895731E-4	2.485178732924845E-5
75-79	0.007157314750823554	4.202211312036556E-4	3.592212895773185E-4	8.517385293751515E-4	3.840730769065669E-4	3.388880090352062E-5
80-84	0.007207018325482051	4.586284388943124E-4	3.6599904975802267E-4	9.195161311821926E-4	6.00961402689099E-4	3.388880090352062E-5
85-89	0.007231870112811299	5.038135067656731E-4	3.908508370872712E-4	9.556641854792814E-4	6.099984162633712E-4	3.388880090352062E-5
90-94	0.007256721900140548	5.264060407013536E-4	4.066656108422474E-4	0.0010121455203184824	6.099984162633712E-4	4.292581447779278E-5
95-99	0.007281573687469798	5.580355882113062E-4	4.2473963799079175E-4	0.0010799231221255237	6.099984162633712E-4	4.518506787136082E-5
100-104	0.007317721741766886	5.806281221469866E-4	4.834802262235608E-4	0.0011138119230290442	6.122576696569392E-4	4.518506787136082E-5
105-109	0.007342573529096133	6.032206560826669E-4	5.377023076691938E-4	0.0011770710180489493	6.212946832312113E-4	4.518506787136082E-5
110-114	0.007362906809638246	6.371094569861877E-4	5.806281221469866E-4	0.0012154783257396062	6.258131900183474E-4	4.518506787136082E-5
115-119	0.007421647397871015	6.777760180704123E-4	6.145169230505072E-4	0.0012538856334302628	6.55183484134732E-4	4.518506787136082E-5
120-124	0.007536869320942985	7.003685520060928E-4	6.709982578897083E-4	0.0012877744343337836	6.664797511025722E-4	4.518506787136082E-5
125-129	0.007704054072067021	7.094055655803649E-4	7.048870587932287E-4	0.0013374780089922805	6.845537782511164E-4	4.7444321264928866E-5
130-134	0.007837350022287536	7.18442579154637E-4	7.342573529096134E-4	0.0013758853166829371	7.003685520060928E-4	5.8740588232769064E-5
135-139	0.007943534931785234	7.568498868452938E-4	7.432943664838855E-4	0.0014142926243735938	7.252203393353412E-4	6.777760180704124E-5
140-144	0.008006794026805139	7.613683936324299E-4	7.591091402388619E-4	0.0014504406786706825	7.342573529096134E-4	7.907386877488144E-5
145-149	0.008103941922728564	7.68146153813134E-4	7.749239139938381E-4	0.001475292465999931	7.342573529096134E-4	7.907386877488144E-5
150-154	0.008194312058471286	7.794424207809743E-4	7.907386877488143E-4	0.0015182182804777237	7.432943664838855E-4	7.907386877488144E-5
155-157	0.008257571153491192	7.907386877488144E-4	8.058003770392679E-4	0.0015249960406584278	7.643807314905207E-4	8.283929109749483E-5
>>END_MODULE
