##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293541
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4164183
Total Bases	678.3 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.81777601992996	39.0	37.0	39.0	35.0	39.0
2	39.50030582229456	40.0	39.0	41.0	37.0	41.0
3	39.57735575021559	40.0	39.0	41.0	37.0	41.0
4	39.600904667254056	40.0	39.0	41.0	37.0	41.0
5	39.616903483828644	40.0	40.0	41.0	37.0	41.0
6	39.596637323575834	40.0	39.0	41.0	37.0	41.0
7	39.61190634513421	40.0	40.0	41.0	37.0	41.0
8	39.58532562089611	40.0	39.0	41.0	37.0	41.0
9	39.577777921863664	40.0	39.0	41.0	37.0	41.0
10-14	39.496009085095444	40.0	39.0	41.0	36.8	41.0
15-19	39.31377328998269	40.0	38.8	41.0	36.0	41.0
20-24	39.0625487880816	40.0	38.0	41.0	35.2	41.0
25-29	38.688286081567504	40.0	37.8	41.0	34.8	41.0
30-34	38.302561342765195	39.8	36.6	40.8	34.2	41.0
35-39	37.53808648659293	38.6	35.2	40.0	33.2	41.0
40-44	36.87761205499375	37.0	35.0	39.8	33.0	41.0
45-49	35.972809456260684	35.2	34.6	38.4	33.0	40.2
50-54	35.25740521009764	35.0	34.0	36.6	32.6	39.4
55-59	34.80817101457838	35.0	34.0	35.6	32.0	38.4
60-64	34.47770422193261	34.8	33.8	35.0	31.8	37.0
65-69	34.497820821035	35.0	33.6	35.0	31.8	37.0
70-74	34.56277032013242	35.0	34.0	35.0	32.0	37.4
75-79	34.57315343730091	35.0	33.2	35.0	32.0	38.0
80-84	34.593790618712	35.0	33.0	35.0	32.0	38.6
85-89	34.48958006888746	34.8	33.0	35.0	31.0	39.0
90-94	38.22309791071814	39.8	37.8	40.6	32.8	40.8
95-99	38.404334914441066	40.0	37.2	41.0	33.0	41.0
100-104	38.223368873130866	40.0	36.8	41.0	33.0	41.0
105-109	38.00115850012406	40.0	36.0	41.0	33.0	41.0
110-114	37.7176520468562	39.6	35.2	41.0	32.0	41.0
115-119	37.349611248436524	38.8	35.0	40.0	32.0	41.0
120-124	36.87143533784924	38.0	34.6	40.0	31.8	41.0
125-129	36.15933280833711	36.6	33.8	39.6	31.0	40.6
130-134	35.63847627997265	35.2	33.8	38.8	31.0	40.0
135-139	35.077931433160174	35.0	33.4	37.2	30.6	39.8
140-144	34.69657791569256	35.0	33.4	35.8	31.0	38.8
145-149	34.26948197512943	35.0	33.0	35.0	31.0	36.8
150-154	33.99272416205225	35.0	33.0	35.0	30.4	36.0
155-159	33.853356073126896	35.0	33.0	35.0	30.2	35.0
160-164	33.75960210335823	35.0	33.0	35.0	30.0	35.0
165-169	33.691202437087014	35.0	33.0	35.0	30.0	35.0
170-174	33.64525834309035	35.0	33.0	35.0	30.0	35.0
175-178	33.38404718848449	34.75	32.75	35.0	29.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	3.0
28	114.0
29	1230.0
30	6621.0
31	28754.0
32	90943.0
33	185714.0
34	347641.0
35	698903.0
36	1252895.0
37	1236886.0
38	296470.0
39	17903.0
40	106.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.284747812476063	22.76946041996713	23.824193125037972	27.121598642518833
2	26.36687676790381	23.466379839694845	24.365307672597485	25.801435719803862
3	24.987038273774232	22.243955176801787	24.838677839086323	27.93032871033766
4	26.115663024415593	21.951748998543053	24.90397276008283	27.028615216958524
5	26.448357336841344	22.662476649080983	23.811441524063664	27.07772449001401
6	26.963992696766688	22.341885551139324	23.10090598804135	27.593215764052637
7	27.08543308495328	22.90720652766701	22.882904041441023	27.124456345938686
8	26.73674523910212	23.041902817431414	23.129891265585588	27.091460677880868
9	27.36616042090369	22.589281018629585	22.429585827520068	27.61497273294665
10-14	27.07672069166989	22.916874690665612	22.762332971437615	27.24407164622688
15-19	27.151770227197026	22.729817589668848	22.74266524790097	27.375746935233153
20-24	26.985960991627888	22.653773861523376	22.889421526383448	27.470843620465285
25-29	27.00170477618299	22.724505623311945	22.750345025662895	27.52344457484217
30-34	27.009446991162495	22.749682230584007	22.784565423757794	27.456305354495708
35-39	26.899187667785014	22.709607142625575	22.84053798788382	27.550667201705593
40-44	27.051817847582587	22.801481106858176	22.785545207787457	27.36115583777178
45-49	27.368331314930206	22.588349263228825	22.567038960583623	27.476280461257346
50-54	27.199990970617765	22.732185401073874	22.653749847208925	27.414073781099436
55-59	27.145031810561637	22.764292539496942	22.70251331413629	27.388162335805127
60-64	27.170237235011047	22.81034719175406	22.602315988514434	27.41709958472046
65-69	27.022237015039924	22.801399458189035	22.729779166765727	27.44658436000531
70-74	26.988631383395013	22.973053777895927	22.690290028079936	27.348024810629116
75-79	27.028312636596425	22.844010457753658	22.63897624095771	27.48870066469221
80-84	26.90421626523138	22.909367815967745	22.6738066026397	27.51260931616118
85-89	26.881527540936602	22.97906696223485	22.615754398882085	27.523651097946466
90-94	25.935808961003147	22.981402277306294	23.983510851530866	27.099277910159685
95-99	26.673796508928827	23.15477251225521	22.851677894955124	27.31975308386084
100-104	26.738887269213652	23.13262890872423	22.794500830364996	27.333982991697127
105-109	26.700710328779003	23.139973248150884	22.831366848473372	27.327949574596737
110-114	26.761973842446352	22.969832834889516	22.770929100358636	27.497264222305496
115-119	26.74536420694534	23.024336056603186	22.695912818213536	27.534386918237942
120-124	26.65558099857935	23.09346097536036	22.78742287005437	27.463535156005918
125-129	26.757733963723407	23.019901358821663	22.68278354923413	27.539581128220796
130-134	26.945732210432706	22.99199575807013	22.566101406548373	27.49617062494879
135-139	27.194120362830432	22.96759930055466	22.442069184799678	27.39621115181523
140-144	27.248693861620737	22.972687048009266	22.452104587092233	27.32651450327776
145-149	27.11212462565596	22.97994672847304	22.533653847930847	27.374274797940156
150-154	27.067050979936468	23.153187308733738	22.571471994007496	27.20828971732229
155-159	27.110590755587012	23.074325480833878	22.511327889985296	27.303755873593815
160-164	26.97595535660423	23.227319377100635	22.567558601197497	27.229166665097637
165-169	26.889330541488874	23.315481885247923	22.644365013325302	27.150822559937897
170-174	26.99040601421776	23.340707486428304	22.556871799391274	27.112014699962657
175-178	26.792056418113276	23.430676015366476	22.63818746965771	27.13908009686254
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.5
15	5.5
16	14.5
17	25.0
18	44.0
19	94.5
20	178.0
21	318.0
22	532.5
23	852.5
24	1272.0
25	1818.5
26	2477.5
27	3232.0
28	4058.5
29	5075.5
30	6196.0
31	7411.0
32	8889.0
33	10468.0
34	12281.5
35	14378.0
36	16781.0
37	19687.5
38	23213.5
39	27645.5
40	32975.5
41	39369.0
42	47091.0
43	56323.5
44	67721.0
45	81169.5
46	96079.0
47	112312.5
48	130149.5
49	148860.0
50	167035.5
51	184496.0
52	199754.5
53	211297.5
54	217636.0
55	218850.5
56	216385.5
57	209691.5
58	198884.5
59	186253.0
60	172575.0
61	157838.5
62	142364.5
63	126501.5
64	111196.0
65	96348.0
66	81938.0
67	68297.5
68	55830.0
69	44236.0
70	34269.0
71	26185.0
72	19320.0
73	13680.0
74	9398.5
75	6234.5
76	3946.5
77	2467.0
78	1493.5
79	882.5
80	487.5
81	250.0
82	137.0
83	80.0
84	40.5
85	16.0
86	4.5
87	3.0
88	2.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1827.0
92-93	3768.0
94-95	5547.0
96-97	7400.0
98-99	8971.0
100-101	10678.0
102-103	12040.0
104-105	13183.0
106-107	14840.0
108-109	16258.0
110-111	16956.0
112-113	18212.0
114-115	19197.0
116-117	19978.0
118-119	20890.0
120-121	21261.0
122-123	22177.0
124-125	23360.0
126-127	24096.0
128-129	25042.0
130-131	25864.0
132-133	27867.0
134-135	104246.0
136-137	88702.0
138-139	79412.0
140-141	82562.0
142-143	84492.0
144-145	79305.0
146-147	78612.0
148-149	80378.0
150-151	80360.0
152-153	81955.0
154-155	82344.0
156-157	73963.0
158-159	76725.0
160-161	76173.0
162-163	75172.0
164-165	77554.0
166-167	76013.0
168-169	77388.0
170-171	84686.0
172-173	78082.0
174-175	83403.0
176-177	165438.0
178-179	1937806.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.78192453586128
#Duplication Level	Percentage of total
1	83.70731232724854
2	11.817391645026188
3	2.406923305826207
4	0.76760181536561
5	0.37013281125113595
6	0.19575443883508128
7	0.1252823086399899
8	0.08668347361984023
9	0.07345849093763475
>10	0.4218954778247683
>50	0.022032537505801832
>100	0.005531367919234166
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.4014314452558882E-5	0.0	0.0	0.0	0.0	0.0
2	3.1218608788326544E-4	0.0	0.0	0.0	0.0	0.0
3	3.1218608788326544E-4	7.204294335767664E-5	0.0	0.0	0.0	0.0
4	3.1218608788326544E-4	7.204294335767664E-5	0.0	0.0	0.0	0.0
5	3.1218608788326544E-4	9.605725781023553E-5	0.0	0.0	0.0	0.0
6	3.1218608788326544E-4	1.2007157226279441E-4	0.0	0.0	0.0	0.0
7	3.1218608788326544E-4	1.2007157226279441E-4	0.0	0.0	0.0	0.0
8	3.1218608788326544E-4	1.2007157226279441E-4	0.0	0.0	0.0	0.0
9	3.1218608788326544E-4	1.2007157226279441E-4	2.4014314452558882E-5	0.0	0.0	0.0
10-14	3.2179181366428903E-4	1.2007157226279443E-4	2.4014314452558882E-5	4.8028628905117765E-6	0.0	0.0
15-19	3.3620040233582437E-4	1.2007157226279443E-4	2.4014314452558882E-5	2.8817177343070657E-5	0.0	0.0
20-24	3.3620040233582437E-4	1.2967729804381798E-4	2.8817177343070657E-5	5.283149179562954E-5	9.605725781023553E-6	0.0
25-29	3.3620040233582437E-4	1.4408588671535328E-4	7.204294335767664E-5	7.204294335767664E-5	2.4014314452558882E-5	0.0
30-34	3.3620040233582437E-4	1.4408588671535328E-4	1.200715722627944E-4	7.204294335767664E-5	2.4014314452558882E-5	0.0
35-39	4.802862890511777E-4	1.4408588671535328E-4	1.4408588671535328E-4	7.204294335767664E-5	5.7634354686141315E-5	0.0
40-44	6.579922160001133E-4	1.4888874960586508E-4	1.5849447538688864E-4	7.204294335767664E-5	8.645153202921198E-5	0.0
45-49	7.972752398249548E-4	1.6810020116791218E-4	1.8731165272995929E-4	7.204294335767664E-5	9.605725781023553E-5	9.605725781023553E-6
50-54	9.749811667738905E-4	1.6810020116791218E-4	2.1132596718251816E-4	7.204294335767664E-5	9.605725781023553E-5	4.8028628905117764E-5
55-59	0.0010998556019271967	1.8731165272995929E-4	2.641574589781477E-4	7.204294335767664E-5	9.605725781023553E-5	7.204294335767664E-5
60-64	0.001119067053489244	1.9211451562047105E-4	2.8817177343070657E-4	7.204294335767664E-5	1.2007157226279443E-4	7.204294335767664E-5
65-69	0.0011622928195038499	1.9211451562047105E-4	2.9297463632121836E-4	7.204294335767664E-5	1.4888874960586508E-4	7.204294335767664E-5
70-74	0.0015513247136353038	1.9211451562047105E-4	3.1218608788326544E-4	7.204294335767664E-5	1.9211451562047105E-4	7.204294335767664E-5
75-79	0.008534687356439427	1.9211451562047105E-4	3.313975394453126E-4	7.204294335767664E-5	1.9211451562047105E-4	7.204294335767664E-5
80-84	0.011839057025111527	2.0172024140149462E-4	3.6501757967889503E-4	8.16486691387002E-5	2.161288300730299E-4	7.204294335767664E-5
85-89	0.011939917145812277	2.3534028163507706E-4	3.8903189413145385E-4	9.605725781023553E-5	2.737631847591712E-4	7.204294335767664E-5
90-94	0.012064791580965581	2.5455173319712416E-4	4.08243345693501E-4	9.605725781023553E-5	2.8817177343070657E-4	7.204294335767664E-5
95-99	0.012122425935651724	2.641574589781477E-4	4.130462085840127E-4	9.605725781023553E-5	2.9297463632121836E-4	7.204294335767664E-5
100-104	0.012136834524323258	2.641574589781477E-4	4.322576601460598E-4	9.605725781023553E-5	3.1218608788326544E-4	7.204294335767664E-5
105-109	0.012165651701666327	2.8336891054019477E-4	4.322576601460598E-4	9.605725781023553E-5	3.2659467655480083E-4	7.204294335767664E-5
110-114	0.012175257427447354	3.1218608788326544E-4	4.322576601460598E-4	9.605725781023553E-5	3.3620040233582437E-4	7.204294335767664E-5
115-119	0.012218483193461959	3.1218608788326544E-4	4.322576601460598E-4	1.3928302382484151E-4	3.5541185389787144E-4	7.204294335767664E-5
120-124	0.012348160491505777	3.1218608788326544E-4	4.466662488175952E-4	1.4408588671535328E-4	3.6021471678838324E-4	7.684580624818842E-5
125-129	0.012655543716498529	3.1218608788326544E-4	4.994977406132248E-4	1.6329733827740041E-4	3.6021471678838324E-4	9.605725781023553E-5
130-134	0.01298213839305333	3.1218608788326544E-4	5.571320952993661E-4	1.6810020116791218E-4	3.6982044256940683E-4	1.2007157226279443E-4
135-139	0.01336156456140376	3.1218608788326544E-4	6.051607242044838E-4	1.6810020116791218E-4	3.9383475702196565E-4	1.2007157226279443E-4
140-144	0.01354887621413372	3.1218608788326544E-4	6.243721757665309E-4	1.6810020116791218E-4	4.08243345693501E-4	1.2007157226279443E-4
145-149	0.013692962100849077	3.1218608788326544E-4	6.243721757665309E-4	1.6810020116791218E-4	4.226519343650363E-4	1.2007157226279443E-4
150-154	0.013880273753579035	3.1218608788326544E-4	6.291750386570427E-4	1.6810020116791218E-4	4.418633859270834E-4	1.2007157226279443E-4
155-159	0.014057979680527969	3.2179181366428903E-4	6.724008046716487E-4	1.6810020116791218E-4	4.610748374891305E-4	1.2007157226279443E-4
160-164	0.014211671293024346	3.3620040233582437E-4	7.204294335767665E-4	1.6810020116791218E-4	4.802862890511777E-4	1.2007157226279443E-4
165-167	0.014344550499661839	3.3620040233582437E-4	7.204294335767665E-4	1.6810020116791218E-4	4.8028628905117765E-4	1.2007157226279441E-4
>>END_MODULE
