##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293542
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4654730
Total Bases	755.9 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.766299441643234	39.0	37.0	39.0	35.0	39.0
2	39.43901794518694	40.0	39.0	41.0	37.0	41.0
3	39.52395756574495	40.0	39.0	41.0	37.0	41.0
4	39.55615320330073	40.0	39.0	41.0	37.0	41.0
5	39.565555467234404	40.0	39.0	41.0	37.0	41.0
6	39.546482395326905	40.0	39.0	41.0	37.0	41.0
7	39.57373338518023	40.0	39.0	41.0	37.0	41.0
8	39.53415643871932	40.0	39.0	41.0	37.0	41.0
9	39.522710876892965	40.0	39.0	41.0	37.0	41.0
10-14	39.454087304741634	40.0	39.0	41.0	36.6	41.0
15-19	39.27805110070831	40.0	38.8	41.0	36.0	41.0
20-24	39.0180289726794	40.0	38.0	41.0	35.2	41.0
25-29	38.651600715830995	40.0	37.8	41.0	34.8	41.0
30-34	38.268638997321	39.6	36.6	40.6	34.0	41.0
35-39	37.51060955200409	38.6	35.2	40.0	33.2	41.0
40-44	36.84428656441942	36.8	35.0	39.8	33.0	41.0
45-49	35.93970730847976	35.2	34.4	38.4	33.0	40.2
50-54	35.22171472888868	35.0	34.0	36.6	32.4	39.2
55-59	34.76969693193804	35.0	34.0	35.4	32.0	38.4
60-64	34.44695563437621	34.8	33.4	35.0	31.8	37.0
65-69	34.46175211021907	35.0	33.0	35.0	31.8	37.0
70-74	34.52784105630187	35.0	33.0	35.0	32.0	37.4
75-79	34.54028216459386	35.0	33.0	35.0	32.0	38.0
80-84	34.564802727548106	35.0	33.0	35.0	31.8	38.6
85-89	34.448052497137326	34.8	33.0	35.0	30.6	39.0
90-94	38.090962464063125	39.8	36.8	40.6	32.8	40.8
95-99	38.28118828930134	40.0	37.0	41.0	33.0	41.0
100-104	38.09466464854858	40.0	36.4	41.0	33.0	41.0
105-109	37.8877070818089	40.0	35.8	41.0	32.2	41.0
110-114	37.601235191972535	39.4	35.0	40.6	32.0	41.0
115-119	37.23664413656955	38.6	35.0	40.0	32.0	41.0
120-124	36.76908218628624	37.8	34.4	40.0	31.2	41.0
125-129	36.06583181466607	36.4	33.6	39.6	30.8	40.6
130-134	35.56128089394667	35.2	33.6	38.8	30.8	40.0
135-139	34.998695984308014	35.0	33.0	37.2	30.4	39.8
140-144	34.63945246536572	35.0	33.0	35.8	31.0	38.8
145-149	34.214076838321	35.0	33.0	35.0	30.4	36.8
150-154	33.949070370717365	35.0	33.0	35.0	30.0	36.0
155-159	33.80560711586456	35.0	33.0	35.0	30.0	35.0
160-164	33.712382354337336	35.0	33.0	35.0	30.0	35.0
165-169	33.64762283111119	35.0	33.0	35.0	30.0	35.0
170-174	33.61480855535824	35.0	33.0	35.0	30.0	35.0
175-178	33.35944450121217	34.75	32.5	35.0	29.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	6.0
28	112.0
29	1325.0
30	7760.0
31	33413.0
32	106779.0
33	224711.0
34	413371.0
35	810038.0
36	1399785.0
37	1325127.0
38	313272.0
39	18904.0
40	127.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.29265284989677	22.761449106607685	23.872211707231138	27.0736863362644
2	26.30584373314886	23.52886203925899	24.420406769028492	25.744887458563653
3	24.900842798615603	22.32092946314824	24.91620351771209	27.862024220524066
4	26.0366551873041	22.00589507876934	24.969697490509652	26.987752243416914
5	26.414894096972326	22.68842661121053	23.89977506751197	26.99690422430517
6	26.86381379800762	22.413351580005713	23.180936380842713	27.541898241143954
7	26.994197300380474	22.952007957497	22.96487658790091	27.08891815422162
8	26.74107842989819	23.071198544276466	23.165124507758776	27.02259851806657
9	27.27275266234561	22.651883138227134	22.501090288803002	27.574273910624246
10-14	27.037168643508863	22.9709392381513	22.810921363859986	27.18097075447985
15-19	27.104777291056624	22.773174813576727	22.78633132319168	27.335716572174974
20-24	26.92883582936067	22.722035434923185	22.928174136845747	27.420954598870395
25-29	26.944862537676727	22.781819783317182	22.818896047676233	27.45442163132985
30-34	26.93840459059924	22.80754415401108	22.83714844899704	27.416902806392635
35-39	26.850515497139472	22.754862258390926	22.903081381734278	27.491540862735324
40-44	27.006176512923414	22.883836441641083	22.826875028197126	27.28311201723838
45-49	27.33412249475265	22.65454709510541	22.621930810165143	27.389399599976798
50-54	27.16317810055578	22.787207850938724	22.71345491575236	27.336159132753135
55-59	27.08471168037673	22.84584927589785	22.737632472775005	27.331806570950413
60-64	27.114384722636974	22.85482938860041	22.65049530262765	27.380290586134965
65-69	26.950521297690738	22.862490412977767	22.788737477791408	27.398250811540088
70-74	26.909006537436113	23.00957090959089	22.77204048355114	27.309382069421854
75-79	26.937025348409037	22.936187491003775	22.72743639265865	27.39935076792854
80-84	26.84262674741607	22.968344028547307	22.750599927385693	27.438429296650934
85-89	26.83636644875213	23.01930294560587	22.685212676138036	27.459117929503968
90-94	25.868576509706187	23.058084748883623	24.019294530145412	27.054044211264777
95-99	26.56038814703681	23.227164944202656	22.92998512461884	27.28246178414169
100-104	26.595829239750252	23.232079738052335	22.87623521212093	27.295855810076485
105-109	26.628891305170455	23.20979018378687	22.902429544138663	27.258888966904017
110-114	26.687897447169362	23.04925460323209	22.828413166190266	27.434434783408285
115-119	26.642933493572897	23.088809912540878	22.766611170445287	27.50164542344094
120-124	26.56606133477974	23.16735732297017	22.849509203909495	27.417072138340593
125-129	26.672756547435572	23.100130389530662	22.760458016026654	27.46665504700711
130-134	26.86649289047344	23.07524827764085	22.632055733946725	27.426203097938977
135-139	27.123556418969535	23.034774479947448	22.5141625992122	27.32750650187082
140-144	27.155505424216358	23.06579114444658	22.51383178700619	27.264871644330867
145-149	27.059555371817485	23.047867327492284	22.589923087230485	27.30265421345975
150-154	26.997305527608596	23.23731779287649	22.64187766372875	27.123499015786166
155-159	27.026618978485033	23.153392714167502	22.59206112558187	27.227927181765594
160-164	26.912930549941965	23.323943615642985	22.635695537410232	27.127430297004818
165-169	26.79749715037027	23.38891172089431	22.73774061715294	27.075850511582484
170-174	26.88618910818445	23.41909885238089	22.644438546568274	27.050273492866385
175-178	26.765229747228343	23.489304377767763	22.680270448868225	27.065195426135674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	8.0
16	13.0
17	24.5
18	50.0
19	103.5
20	213.0
21	392.5
22	666.5
23	1043.0
24	1527.5
25	2148.0
26	2867.0
27	3746.0
28	4865.0
29	6138.0
30	7296.5
31	8574.0
32	10168.5
33	12125.5
34	14234.0
35	16571.0
36	19450.0
37	22825.0
38	26913.0
39	31866.0
40	37974.0
41	45121.5
42	53825.0
43	64643.5
44	77290.0
45	92228.5
46	108772.5
47	127259.0
48	147435.5
49	168245.0
50	188759.0
51	207902.0
52	224334.5
53	236788.0
54	243950.5
55	244556.5
56	240734.0
57	232798.5
58	220701.5
59	205888.0
60	190113.5
61	174008.5
62	156821.0
63	139607.0
64	122623.5
65	105892.5
66	90101.0
67	74991.5
68	60852.5
69	48302.5
70	37439.5
71	28302.0
72	20877.5
73	14906.5
74	10272.5
75	6860.0
76	4496.5
77	2845.5
78	1687.5
79	972.0
80	552.0
81	304.0
82	157.5
83	80.5
84	43.0
85	20.5
86	9.5
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2019.0
92-93	4598.0
94-95	6650.0
96-97	8621.0
98-99	10572.0
100-101	12249.0
102-103	13775.0
104-105	15628.0
106-107	17356.0
108-109	18909.0
110-111	20164.0
112-113	20889.0
114-115	21720.0
116-117	22961.0
118-119	24215.0
120-121	25359.0
122-123	25864.0
124-125	27062.0
126-127	27894.0
128-129	28897.0
130-131	30131.0
132-133	32441.0
134-135	118192.0
136-137	104382.0
138-139	93919.0
140-141	95065.0
142-143	92570.0
144-145	96757.0
146-147	93849.0
148-149	92512.0
150-151	95305.0
152-153	90691.0
154-155	87107.0
156-157	86981.0
158-159	83892.0
160-161	86303.0
162-163	88965.0
164-165	90689.0
166-167	89636.0
168-169	89058.0
170-171	91699.0
172-173	89345.0
174-175	96846.0
176-177	188201.0
178-179	2094792.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.86332411643538
#Duplication Level	Percentage of total
1	82.18118817967188
2	12.741073552929683
3	2.663280905464039
4	0.8942930013204209
5	0.44313211789361207
6	0.23446327344356574
7	0.13283394409447094
8	0.10203155877198222
9	0.06838267741033446
>10	0.506667158284709
>50	0.02455374518206183
>100	0.008099885533298809
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.445056963561797E-5	0.0	2.1483523211872656E-5	0.0	0.0	0.0
2	4.0818694102558043E-4	0.0	2.1483523211872656E-5	0.0	0.0	0.0
3	4.0818694102558043E-4	4.296704642374531E-5	2.1483523211872656E-5	0.0	0.0	0.0
4	4.0818694102558043E-4	6.445056963561797E-5	4.296704642374531E-5	0.0	0.0	0.0
5	4.0818694102558043E-4	8.593409284749062E-5	6.445056963561797E-5	0.0	0.0	0.0
6	4.0818694102558043E-4	8.593409284749062E-5	8.593409284749062E-5	0.0	0.0	0.0
7	4.0818694102558043E-4	8.593409284749062E-5	8.593409284749062E-5	0.0	0.0	0.0
8	4.0818694102558043E-4	8.593409284749062E-5	8.593409284749062E-5	0.0	2.1483523211872656E-5	0.0
9	4.0818694102558043E-4	8.593409284749062E-5	8.593409284749062E-5	0.0	2.1483523211872656E-5	0.0
10-14	4.0818694102558043E-4	9.452750213223969E-5	9.452750213223969E-5	4.296704642374531E-6	2.1483523211872656E-5	0.0
15-19	4.2537375959507856E-4	1.1171432070173782E-4	1.246044346288614E-4	4.296704642374531E-5	3.867034178137078E-5	0.0
20-24	4.5115398744932574E-4	1.6327477641023218E-4	1.546813671254831E-4	4.296704642374531E-5	6.015386499324343E-5	0.0
25-29	4.683408060188238E-4	1.7186818569498124E-4	1.9764841354922847E-4	5.156045570849437E-5	6.445056963561797E-5	0.0
30-34	5.070111478001946E-4	1.7186818569498124E-4	2.1483523211872656E-4	6.445056963561797E-5	6.445056963561797E-5	0.0
35-39	6.616925149256777E-4	1.7186818569498124E-4	2.449121646153483E-4	6.445056963561797E-5	6.87472742779925E-5	0.0
40-44	8.937145656139024E-4	2.062418228339775E-4	2.8358250639671904E-4	6.445056963561797E-5	1.4608795784073407E-4	0.0
45-49	0.0010054288863156403	2.5350557390009726E-4	3.1365943889334077E-4	6.445056963561797E-5	1.8905500426447938E-4	0.0
50-54	0.0011472201395139998	2.5780227854247187E-4	3.351429621052134E-4	6.445056963561797E-5	2.1483523211872656E-4	0.0
55-59	0.0012030772998648687	2.792858017543445E-4	3.995935317408314E-4	9.882420677461424E-5	2.1483523211872656E-4	0.0
60-64	0.001263231164858112	2.792858017543445E-4	4.468572828069512E-4	1.1171432070173782E-4	2.2772534604585012E-4	0.0
65-69	0.001336275143778479	2.792858017543445E-4	4.769342153035729E-4	1.4179125319835952E-4	2.535055739000973E-4	0.0
70-74	0.0017530554940888086	2.792858017543445E-4	5.370880802968164E-4	1.503846624831086E-4	2.792858017543445E-4	0.0
75-79	0.009164871002184874	2.792858017543445E-4	5.58571603508689E-4	1.503846624831086E-4	3.222528481780898E-4	2.5780227854247186E-5
80-84	0.01231865220968778	2.8787921103909354E-4	5.843518313629362E-4	1.546813671254831E-4	3.566264853170861E-4	4.296704642374531E-5
85-89	0.01235732255146915	3.437363713899625E-4	6.359122870714304E-4	1.8475829962210486E-4	3.995935317408314E-4	4.296704642374531E-5
90-94	0.012421773121104769	4.2537375959507856E-4	6.659892195680523E-4	1.933517089068539E-4	4.0818694102558043E-4	4.296704642374531E-5
95-99	0.012473333576813263	4.296704642374531E-4	7.089562659917976E-4	2.1483523211872653E-4	4.0818694102558043E-4	4.296704642374531E-5
100-104	0.012490520395382761	4.296704642374531E-4	7.218463799189211E-4	2.5780227854247187E-4	4.339671688798276E-4	4.296704642374531E-5
105-109	0.012512003918594633	4.296704642374531E-4	7.562200170579174E-4	2.8787921103909354E-4	4.5115398744932574E-4	4.296704642374531E-5
110-114	0.012602234716084498	4.296704642374531E-4	7.948903588392882E-4	3.2654955282046435E-4	4.5115398744932574E-4	4.296704642374531E-5
115-119	0.012679575399647241	4.296704642374531E-4	8.2496729133591E-4	3.652198946018351E-4	4.5115398744932574E-4	4.296704642374531E-5
120-124	0.012825663357487974	4.296704642374531E-4	8.808244516867788E-4	3.9529682709845684E-4	4.5115398744932574E-4	4.296704642374531E-5
125-129	0.013083465636030448	4.296704642374531E-4	9.023079748986515E-4	4.296704642374531E-4	4.5115398744932574E-4	4.296704642374531E-5
130-134	0.01332408109600342	4.296704642374531E-4	9.023079748986515E-4	4.3826387352220215E-4	4.597473967340748E-4	4.296704642374531E-5
135-139	0.01359047678383064	4.296704642374531E-4	9.109013841834006E-4	4.5115398744932574E-4	4.7263751066119837E-4	4.296704642374531E-5
140-144	0.013869762585584986	4.296704642374531E-4	9.538684306071458E-4	4.5115398744932574E-4	4.7263751066119837E-4	5.156045570849437E-5
145-149	0.01406311429449184	4.296704642374531E-4	9.753519538190185E-4	4.5115398744932574E-4	4.94121033873071E-4	6.445056963561797E-5
150-154	0.01423498248018682	4.425605781645767E-4	9.925387723885167E-4	4.5115398744932574E-4	5.027144431578201E-4	6.445056963561797E-5
155-159	0.01436818032410043	4.5115398744932574E-4	0.001035505818812262	4.5115398744932574E-4	5.156045570849437E-4	6.445056963561797E-5
160-164	0.01457442214693441	4.597473967340748E-4	0.0010655827513088836	4.5115398744932574E-4	5.284946710120673E-4	6.445056963561797E-5
165-167	0.014680407528112982	4.7263751066119837E-4	0.0010884985094015479	4.5115398744932574E-4	5.370880802968163E-4	6.445056963561797E-5
>>END_MODULE
