##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293543
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3828596
Total Bases	617.8 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.68201476468136	39.0	37.0	39.0	35.0	39.0
2	39.35854997497777	40.0	39.0	41.0	36.0	41.0
3	39.45687949316146	40.0	39.0	41.0	37.0	41.0
4	39.50398109385268	40.0	39.0	41.0	37.0	41.0
5	39.5150261349069	40.0	39.0	41.0	37.0	41.0
6	39.50355717866288	40.0	39.0	41.0	37.0	41.0
7	39.52926529725257	40.0	39.0	41.0	37.0	41.0
8	39.505045452693366	40.0	39.0	41.0	37.0	41.0
9	39.495273985555016	40.0	39.0	41.0	37.0	41.0
10-14	39.40890315927823	40.0	39.0	41.0	36.4	41.0
15-19	39.22231052845482	40.0	38.8	41.0	36.0	41.0
20-24	38.96759574528104	40.0	38.0	41.0	35.0	41.0
25-29	38.603551327954165	40.0	37.6	40.6	34.6	41.0
30-34	38.18505060340657	39.6	36.6	40.0	34.0	41.0
35-39	37.38819739664358	38.2	35.0	40.0	33.0	41.0
40-44	36.698480591840976	36.6	35.0	39.6	33.0	41.0
45-49	35.78017952272844	35.2	34.0	38.0	32.8	40.0
50-54	35.06791011639776	35.0	34.0	36.4	32.0	39.2
55-59	34.630857212408934	35.0	33.2	35.2	32.0	37.8
60-64	34.315380886361474	34.8	33.0	35.0	31.6	37.0
65-69	34.33177979604012	35.0	33.0	35.0	31.8	37.0
70-74	34.39754369486883	35.0	33.0	35.0	32.0	37.2
75-79	34.41806239153988	35.0	33.0	35.0	31.4	38.0
80-84	34.43196519037266	35.0	33.0	35.0	31.0	38.2
85-89	34.316636072335655	34.8	32.8	35.0	30.6	39.0
90-94	37.61933296849211	39.8	35.8	40.6	31.6	40.6
95-99	37.85456727091342	40.0	36.0	41.0	32.0	41.0
100-104	37.67123084478172	40.0	35.0	41.0	32.0	41.0
105-109	37.44926186343912	39.4	35.0	40.4	31.0	41.0
110-114	37.165063158177574	39.0	34.8	40.0	31.0	41.0
115-119	36.81027658469301	38.0	34.0	40.0	30.6	41.0
120-124	36.35536784629504	37.2	34.0	40.0	30.0	41.0
125-129	35.68291415910079	35.8	33.0	39.6	30.0	40.6
130-134	35.175742040012764	35.0	33.0	38.6	30.0	40.0
135-139	34.65501125997328	35.0	33.0	36.8	30.0	39.6
140-144	34.32850649388633	35.0	33.0	35.6	30.0	38.4
145-149	33.940075794661105	35.0	33.0	35.0	30.0	36.6
150-154	33.695542135288335	35.0	33.0	35.0	30.0	35.8
155-159	33.56331742563189	35.0	33.0	35.0	30.0	35.0
160-164	33.4812811041691	35.0	33.0	35.0	30.0	35.0
165-169	33.419034346413994	34.8	33.0	35.0	30.0	35.0
170-174	33.38885856034883	34.2	33.0	35.0	30.0	35.0
175-178	33.150844947015585	34.0	32.5	35.0	29.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	5.0
28	134.0
29	1800.0
30	9875.0
31	41537.0
32	129523.0
33	243733.0
34	388692.0
35	701396.0
36	1120341.0
37	968308.0
38	211255.0
39	11944.0
40	53.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.26333517561007	22.802118583418046	23.866686377982948	27.06785986298894
2	26.297159585393704	23.52468111025556	24.43728719352995	25.740872110820785
3	24.863762068392695	22.34881925384658	24.948623464058365	27.83879521370236
4	26.053519358010092	22.02750042051969	24.98913439809267	26.92984582337755
5	26.37572624533902	22.73102202478402	23.884055669493463	27.009196060383495
6	26.829913628912532	22.451023821787413	23.17442216415626	27.544640385143797
7	26.98130071702525	22.96949064356751	22.96209889996228	27.087109739444955
8	26.682052637572625	23.103769632523257	23.199601107037672	27.01457662286645
9	27.273026456695877	22.64728375623858	22.523086792129543	27.556602994936004
10-14	27.013824388888253	22.983265928293296	22.850130961846066	27.152778720972385
15-19	27.09083956625353	22.812331204441524	22.81976996267039	27.277059266634556
20-24	26.901866898466174	22.749044297178393	22.955845955018496	27.393242849336936
25-29	26.950840464755228	22.821159505991233	22.809343163917006	27.418656865336537
30-34	26.935524145143546	22.7953745968496	22.872342759591245	27.396758498415608
35-39	26.833538978779686	22.79974173300082	22.894006053393987	27.47271323482551
40-44	26.992693927486734	22.86966814989098	22.84518397866999	27.2924539439523
45-49	27.3187455662598	22.649639711267525	22.630379387117365	27.401235335355313
50-54	27.16413536450438	22.813475226950036	22.698022982837575	27.324366425708014
55-59	27.091132101689496	22.852042889873992	22.741239869654567	27.315585138781945
60-64	27.130838563274896	22.884159101665468	22.63177937813235	27.353222956927294
65-69	26.930404775014132	22.885799389645708	22.78764852703184	27.396147308308322
70-74	26.900498250533616	23.044964786046897	22.76065168536978	27.293885278049707
75-79	26.916472774876222	22.94499602465238	22.720819851454685	27.417711349016717
80-84	26.816185358810387	22.990302450297705	22.736648108079308	27.456864082812604
85-89	26.79571310213979	23.05124907407311	22.672441803731708	27.480596020055394
90-94	25.8134315626082	23.110337114641894	24.031539734683943	27.044691588065962
95-99	26.47578280968056	23.290792675207108	22.98357731513138	27.24984719998095
100-104	26.542707988688523	23.273842033025748	22.91007653027571	27.27337344801002
105-109	26.525663886390127	23.280128385966968	22.925209319160487	27.268998408482414
110-114	26.588144074052412	23.124441969180744	22.851991351023457	27.435422605743394
115-119	26.563037615105667	23.129206393240832	22.822308814428315	27.485447177225193
120-124	26.503119768613242	23.215769830650295	22.864792405634173	27.416317995102286
125-129	26.607720442791145	23.145157941797343	22.782491906215764	27.46462970919574
130-134	26.806632592476333	23.13385121099587	22.61582292281324	27.44369327371456
135-139	27.126268917998704	23.047370668149984	22.50101161416852	27.325348799682793
140-144	27.149768042580845	23.084389707047613	22.516994810575422	27.24884743979612
145-149	27.027505892734567	23.106688452872323	22.572591632628605	27.2932140217645
150-154	26.975411853416	23.27254610580929	22.58055095046256	27.17149109031215
155-159	26.99964695200558	23.171336041374975	22.591726131261474	27.23729087535797
160-164	26.898633462732572	23.332567851744386	22.607098883795274	27.161699801727767
165-169	26.793517552773892	23.422865694620565	22.716883952084675	27.066732800520864
170-174	26.865418392723022	23.43573459658306	22.61902254022239	27.079824470471532
175-178	26.7241612700025	23.495558764124212	22.685027606704253	27.095252359169038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.5
13	2.5
14	3.0
15	5.0
16	11.5
17	20.0
18	45.0
19	94.5
20	185.0
21	330.5
22	550.5
23	884.0
24	1309.5
25	1831.0
26	2520.0
27	3285.5
28	4134.0
29	5139.0
30	6202.5
31	7362.5
32	8745.5
33	10253.5
34	11945.5
35	13856.0
36	16172.5
37	18955.5
38	22316.5
39	26600.5
40	31485.5
41	37394.0
42	44616.5
43	53031.0
44	63500.0
45	75642.5
46	89401.5
47	104787.5
48	120803.0
49	137984.5
50	155384.5
51	170869.0
52	184101.0
53	194283.5
54	200219.0
55	201701.5
56	198326.5
57	191094.0
58	181563.0
59	170024.5
60	156740.0
61	142802.5
62	128829.0
63	114836.0
64	100728.5
65	86837.5
66	73951.5
67	61728.0
68	50150.0
69	39685.5
70	30685.5
71	23028.0
72	16788.5
73	12017.0
74	8203.0
75	5376.0
76	3469.5
77	2127.0
78	1256.5
79	732.0
80	402.0
81	228.0
82	118.5
83	50.0
84	31.0
85	21.5
86	6.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1779.0
92-93	3924.0
94-95	5568.0
96-97	7208.0
98-99	8996.0
100-101	10652.0
102-103	12024.0
104-105	13333.0
106-107	15020.0
108-109	16285.0
110-111	17116.0
112-113	18176.0
114-115	18798.0
116-117	20120.0
118-119	20888.0
120-121	21834.0
122-123	22102.0
124-125	23083.0
126-127	23958.0
128-129	24947.0
130-131	25837.0
132-133	27785.0
134-135	104938.0
136-137	95373.0
138-139	82438.0
140-141	85757.0
142-143	87997.0
144-145	82843.0
146-147	84473.0
148-149	87071.0
150-151	83299.0
152-153	73881.0
154-155	76405.0
156-157	76035.0
158-159	73425.0
160-161	87968.0
162-163	71661.0
164-165	71592.0
166-167	75068.0
168-169	75784.0
170-171	75702.0
172-173	74844.0
174-175	77333.0
176-177	150895.0
178-179	1614381.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.30177373312694
#Duplication Level	Percentage of total
1	84.61663612789494
2	11.197892778939734
3	2.2420273663618544
4	0.7274864898812379
5	0.3181209781066292
6	0.18506964701881232
7	0.12606150722359374
8	0.08076319062280632
9	0.06190551377322142
>10	0.4182351897605631
>50	0.022761352609303696
>100	0.003039857807319771
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	2.611923535416116E-5	0.0	0.0	0.0
2	4.1790776566657856E-4	0.0	2.611923535416116E-5	0.0	0.0	0.0
3	4.701462363749009E-4	5.223847070832232E-5	2.611923535416116E-5	0.0	0.0	0.0
4	4.701462363749009E-4	5.223847070832232E-5	2.611923535416116E-5	0.0	0.0	0.0
5	4.701462363749009E-4	5.223847070832232E-5	5.223847070832232E-5	0.0	0.0	0.0
6	4.701462363749009E-4	5.223847070832232E-5	5.223847070832232E-5	2.611923535416116E-5	0.0	0.0
7	4.701462363749009E-4	7.835770606248349E-5	5.223847070832232E-5	2.611923535416116E-5	0.0	0.0
8	4.701462363749009E-4	7.835770606248349E-5	5.223847070832232E-5	2.611923535416116E-5	0.0	0.0
9	4.701462363749009E-4	7.835770606248349E-5	5.223847070832232E-5	2.611923535416116E-5	0.0	0.0
10-14	4.858177775873976E-4	7.835770606248349E-5	7.313385899165124E-5	2.611923535416116E-5	0.0	0.0
15-19	4.96265471729062E-4	7.835770606248349E-5	1.0447694141664464E-4	2.611923535416116E-5	0.0	0.0
20-24	5.014893187998942E-4	9.402924727498019E-5	1.0447694141664464E-4	4.179077656665785E-5	0.0	0.0
25-29	5.328324012248877E-4	1.0447694141664464E-4	1.305961767708058E-4	5.223847070832232E-5	0.0	0.0
30-34	5.537277895082166E-4	1.0447694141664464E-4	1.305961767708058E-4	6.791001192081902E-5	0.0	0.0
35-39	6.320854955707E-4	1.0447694141664464E-4	1.5149156505413474E-4	9.402924727498019E-5	0.0	2.0895388283328926E-5
40-44	8.462632254748216E-4	1.0447694141664464E-4	1.932823416207926E-4	1.0447694141664464E-4	5.223847070832232E-6	5.223847070832232E-5
45-49	9.873070963872918E-4	1.305961767708058E-4	2.3507311818745047E-4	1.253723296999736E-4	2.611923535416116E-5	5.223847070832232E-5
50-54	0.0010395455670956142	1.305961767708058E-4	2.5596850647077935E-4	1.305961767708058E-4	2.611923535416116E-5	6.791001192081902E-5
55-59	0.0011962609792205811	1.305961767708058E-4	2.611923535416116E-4	1.305961767708058E-4	5.746231777915455E-5	7.835770606248349E-5
60-64	0.001305961767708058	1.3582002384163804E-4	2.7686389475410834E-4	1.4104387091247027E-4	9.925309434581243E-5	7.835770606248349E-5
65-69	0.0013216333089205547	1.5671541212496697E-4	2.8731158889577275E-4	1.7238695333746367E-4	1.0447694141664464E-4	1.0447694141664464E-4
70-74	0.0016141687448871598	1.5671541212496697E-4	3.029831301082695E-4	1.828346474791281E-4	1.0447694141664464E-4	1.0447694141664464E-4
75-79	0.007485772852502588	1.6716310626663144E-4	3.60445447887424E-4	2.0373003576245707E-4	1.0447694141664464E-4	1.305961767708058E-4
80-84	0.009961876364077065	1.985061886916248E-4	4.1790776566657856E-4	2.0895388283328928E-4	1.2014848262914134E-4	1.305961767708058E-4
85-89	0.010003667140643725	2.4029696525828268E-4	4.440270010207397E-4	2.298492711166182E-4	2.4029696525828268E-4	1.305961767708058E-4
90-94	0.010003667140643725	2.8731158889577275E-4	4.440270010207397E-4	2.3507311818745047E-4	2.611923535416116E-4	1.305961767708058E-4
95-99	0.010019338681856222	3.1343082424993394E-4	4.858177775873976E-4	2.3507311818745047E-4	2.611923535416116E-4	1.305961767708058E-4
100-104	0.010029786375997886	3.1343082424993394E-4	5.223847070832232E-4	2.3507311818745047E-4	2.611923535416116E-4	1.305961767708058E-4
105-109	0.010097696387918704	3.3432621253326293E-4	5.537277895082166E-4	2.611923535416116E-4	2.611923535416116E-4	1.305961767708058E-4
110-114	0.01013426331741453	3.395500596040951E-4	5.955185660748744E-4	2.6641620061244387E-4	2.611923535416116E-4	1.305961767708058E-4
115-119	0.01013426331741453	3.395500596040951E-4	6.007424131457067E-4	2.8731158889577275E-4	2.8731158889577275E-4	1.305961767708058E-4
120-124	0.010264859494185336	3.395500596040951E-4	6.007424131457067E-4	2.8731158889577275E-4	2.977592830374372E-4	1.305961767708058E-4
125-129	0.010562618777222772	3.395500596040951E-4	6.164139543582035E-4	2.8731158889577275E-4	3.1343082424993394E-4	1.305961767708058E-4
130-134	0.01086037806026021	3.395500596040951E-4	6.52980883854029E-4	2.8731158889577275E-4	3.4999775374575955E-4	1.305961767708058E-4
135-139	0.011105898872589327	3.395500596040951E-4	6.52980883854029E-4	2.8731158889577275E-4	4.0746007152491414E-4	1.305961767708058E-4
140-144	0.011252166590572628	3.395500596040951E-4	6.52980883854029E-4	2.8731158889577275E-4	4.1790776566657856E-4	1.305961767708058E-4
145-149	0.011335748143705944	3.395500596040951E-4	6.52980883854029E-4	2.8731158889577275E-4	4.440270010207397E-4	1.305961767708058E-4
150-154	0.011419329696839259	3.395500596040951E-4	6.582047309248611E-4	2.8731158889577275E-4	4.7014623637490083E-4	1.305961767708058E-4
155-159	0.011555149720680896	3.552216008165918E-4	6.791001192081902E-4	3.029831301082695E-4	5.067131658707264E-4	1.305961767708058E-4
160-164	0.011764103603514185	3.656692949582562E-4	7.104432016331836E-4	3.1343082424993394E-4	5.223847070832232E-4	1.305961767708058E-4
165-167	0.01192778414506693	3.656692949582562E-4	7.487514134859532E-4	3.1343082424993394E-4	5.397975306526639E-4	1.305961767708058E-4
>>END_MODULE
