##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293545
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7669488
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.190441135053604	41.0	40.0	41.0	39.0	41.0
2	40.28526141510359	41.0	40.0	41.0	39.0	41.0
3	40.324634577953574	41.0	40.0	41.0	39.0	41.0
4	40.33019622691893	41.0	40.0	41.0	39.0	41.0
5	40.336455445265706	41.0	40.0	41.0	39.0	41.0
6	40.35069068495837	41.0	40.0	41.0	39.0	41.0
7	40.33747024573218	41.0	40.0	41.0	39.0	41.0
8	40.3407124439076	41.0	40.0	41.0	39.0	41.0
9	40.301529776172806	41.0	40.0	41.0	39.0	41.0
10-14	40.27948666195188	41.0	40.0	41.0	39.0	41.0
15-19	40.19865104424181	41.0	40.0	41.0	38.2	41.0
20-24	40.08299654422825	41.0	40.0	41.0	38.0	41.0
25-29	39.91120833620184	41.0	40.0	41.0	37.8	41.0
30-34	39.66465829270481	41.0	39.4	41.0	36.6	41.0
35-39	39.300387613879835	40.0	38.6	41.0	35.2	41.0
40-44	38.92201489851734	40.0	37.6	41.0	35.0	41.0
45-49	38.479828118904415	39.6	36.2	41.0	35.0	41.0
50-54	37.902835234894425	38.4	35.2	41.0	35.0	41.0
55-59	37.15828081353019	36.8	35.0	39.6	35.0	41.0
60-64	36.20896597008822	35.6	34.8	38.2	34.2	40.2
65-69	35.83321074366372	35.0	35.0	36.8	34.0	39.0
70-74	35.45334671623451	35.0	35.0	36.0	34.0	37.6
75-79	35.21127989247783	35.0	35.0	35.4	34.0	36.8
80-84	35.10315744675525	35.0	35.0	35.0	34.0	36.0
85-89	35.97952716009204	36.2	35.8	36.2	34.6	37.0
90-94	40.135221661125236	41.0	40.0	41.0	38.0	41.0
95-99	40.10995870938972	41.0	40.0	41.0	38.0	41.0
100-104	40.01383591380782	41.0	40.0	41.0	38.0	41.0
105-109	39.89617779039506	41.0	40.0	41.0	37.8	41.0
110-114	39.729913809715995	41.0	40.0	41.0	37.0	41.0
115-119	39.48953196474915	40.6	39.0	41.0	35.8	41.0
120-124	39.19225059326689	40.0	38.4	41.0	35.0	41.0
125-129	38.297246184149806	39.2	37.0	40.4	34.6	40.6
130-134	38.614183328373585	40.0	36.6	41.0	35.0	41.0
135-139	38.187780214509885	39.0	35.4	41.0	35.0	41.0
140-144	37.49415754510346	37.4	35.0	40.6	35.0	41.0
145-149	36.733474556509464	36.2	35.0	39.0	34.6	41.0
150-154	36.02958163834313	35.0	35.0	37.0	34.0	39.4
155-159	35.46597128383708	35.0	35.0	36.0	34.0	37.8
160-164	35.091374082202684	35.0	35.0	35.4	34.0	36.6
165-169	34.880793351837255	35.0	35.0	35.0	34.0	36.0
170-174	34.774205465875944	35.0	35.0	35.0	34.0	36.0
175-176	34.53462642093411	35.0	34.5	35.0	33.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	37.0
30	332.0
31	2340.0
32	11358.0
33	39058.0
34	106235.0
35	269673.0
36	798692.0
37	2053797.0
38	3176343.0
39	1191777.0
40	19844.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.474746684524444	28.2007742889747	28.34820264403569	20.976276382465166
2	20.25270787306793	27.39105922064159	30.121306663495663	22.234926242794824
3	20.9896149521324	26.602010460150666	30.387673857759474	22.02070072995746
4	21.84736451768358	27.492395841808477	28.487051547639165	22.17318809286878
5	21.93210289917658	27.70093649015423	28.234322812683192	22.132637797985993
6	22.008261829212067	27.91393636706909	27.89080574870187	22.186996055016973
7	22.13867470683832	28.175114166682313	27.6789271982693	22.007283928210068
8	22.330356341909656	28.088211364304893	27.62587280924098	21.95555948454447
9	22.049959527937197	28.09878573380648	27.744224907842607	22.107029830413712
10-14	22.160441479274755	27.961397162365987	27.785854805431605	22.092306552927653
15-19	22.11963562626345	27.92735577655249	27.820323859949976	22.13268473723409
20-24	22.16713292986442	27.753714459165984	27.829351842000406	22.249800768969195
25-29	22.214332951560785	27.83636404411872	27.669406354113864	22.279896650206638
30-34	22.114946916925877	27.85154889087772	27.775921938987324	22.25758225320908
35-39	22.077064335976534	27.849851254738255	27.816023703277192	22.257060706008012
40-44	22.15791979855761	27.887473062087064	27.723532522640365	22.231074616714963
45-49	22.23839453168191	27.77195035705121	27.674869561045014	22.314785550221867
50-54	22.274615984795858	27.813556785016157	27.6753024452219	22.23652478496609
55-59	22.234866264866703	27.790509614201103	27.695608885495353	22.279015235436837
60-64	22.19671769484482	27.854279190475296	27.690724595957384	22.258278518722502
65-69	22.190427835599976	27.76370208806637	27.746720511199708	22.299149565133945
70-74	22.200947442645454	27.86565152719451	27.691551248271075	22.241849781888963
75-79	22.143089603895334	27.816743438414665	27.719164564831445	22.321002392858556
80-84	22.185319280765547	27.837787867977625	27.69202064075203	22.284872210504794
85-89	22.213412420750902	27.922995641951587	27.793717129487653	22.069874807809857
90-94	21.346792509673357	27.511875498376615	28.997134295362752	22.144197696587277
95-99	22.0478116719855	28.164674298414322	27.794590555102396	21.992923474497783
100-104	22.001925496815282	28.000935147304634	27.84775816503689	22.149381190843194
105-109	22.070337787455045	27.91569798147445	27.885149755331824	22.128814475738682
110-114	22.088072378525734	27.806786822590684	27.817557062364717	22.287583736518865
115-119	22.113717074879244	27.916321721612942	27.70425876352917	22.26570243997864
120-124	22.033311688929995	27.853164353724647	27.855931498540965	22.257592458804396
125-129	22.08562928265101	27.93849071333998	27.7727471739119	22.203132830097115
130-134	22.1554028012553	27.82057099946978	27.748566499406845	22.275459699868076
135-139	22.223439960758018	27.835832119377844	27.739760607467435	22.200967312396703
140-144	22.185249677605075	27.87215242911452	27.752518964870347	22.190078928410053
145-149	22.158921644196923	27.895462133287324	27.733479115168358	22.2121371073474
150-154	22.116070646415416	27.95105547285237	27.814112171557635	22.118761709174574
155-159	22.05622453991275	28.00345823823419	27.831826192471038	22.108491029382023
160-164	22.023723273576003	28.043866488860953	27.85499551020747	22.077414727355574
165-169	21.99942248057451	28.006063112803265	27.912675842408095	22.08183856421413
170-174	21.941959305848677	28.166770280011278	27.906704057462576	21.984566356677465
175-176	21.89910195539549	28.12971640729619	27.864299756005572	22.106881881302744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	3.5
11	6.0
12	11.5
13	28.0
14	59.0
15	141.5
16	301.5
17	562.0
18	1078.0
19	2027.5
20	3551.5
21	5931.5
22	9373.5
23	14274.5
24	20851.5
25	29050.5
26	39179.0
27	51558.0
28	66172.5
29	82038.5
30	98980.0
31	118056.0
32	138347.0
33	159320.0
34	181426.5
35	204240.5
36	227845.0
37	251482.5
38	273888.5
39	295387.0
40	314280.0
41	328902.0
42	339323.5
43	344190.5
44	343646.5
45	338429.5
46	327009.5
47	310235.5
48	290686.0
49	268629.0
50	245220.0
51	221914.5
52	199393.5
53	178400.5
54	159574.5
55	143421.5
56	129672.0
57	117848.0
58	107529.5
59	98051.5
60	89260.0
61	80872.5
62	72810.0
63	64686.5
64	56676.5
65	48955.5
66	40958.0
67	33598.5
68	27051.0
69	21032.5
70	15964.0
71	11826.5
72	8496.0
73	5878.0
74	3918.0
75	2521.0
76	1562.0
77	969.0
78	586.5
79	331.0
80	166.0
81	83.0
82	41.5
83	18.5
84	10.0
85	3.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	478.0
92-93	955.0
94-95	1534.0
96-97	2127.0
98-99	2398.0
100-101	2695.0
102-103	3402.0
104-105	4029.0
106-107	4603.0
108-109	5250.0
110-111	5505.0
112-113	7697.0
114-115	5693.0
116-117	6348.0
118-119	6689.0
120-121	6053.0
122-123	8119.0
124-125	9808.0
126-127	6967.0
128-129	8471.0
130-131	8293.0
132-133	32379.0
134-135	51795.0
136-137	63250.0
138-139	55518.0
140-141	54162.0
142-143	52804.0
144-145	55186.0
146-147	49635.0
148-149	51390.0
150-151	52153.0
152-153	47018.0
154-155	47905.0
156-157	52362.0
158-159	51504.0
160-161	51524.0
162-163	51337.0
164-165	59176.0
166-167	52914.0
168-169	54040.0
170-171	57558.0
172-173	56772.0
174-175	105348.0
176-177	6356644.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.92786961624272
#Duplication Level	Percentage of total
1	85.04137188316382
2	12.040229960029553
3	2.0359155296405724
4	0.5092611530278288
5	0.17735732647153898
6	0.07532724716496891
7	0.025965050509657275
8	0.022997757606118163
9	0.012625582618970585
>10	0.058948509766968096
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.387849619166234E-4	0.0	0.0	7.823208015971862E-5	0.0	0.0
2	9.387849619166234E-4	0.0	0.0	9.127076018633838E-5	0.0	0.0
3	9.387849619166234E-4	0.0	1.3038680026619768E-5	1.0430944021295815E-4	1.3038680026619768E-5	0.0
4	9.387849619166234E-4	0.0	1.3038680026619768E-5	1.0430944021295815E-4	1.3038680026619768E-5	0.0
5	9.518236419432431E-4	1.3038680026619768E-5	2.6077360053239537E-5	1.0430944021295815E-4	1.3038680026619768E-5	0.0
6	9.518236419432431E-4	1.3038680026619768E-5	2.6077360053239537E-5	1.1734812023957793E-4	1.3038680026619768E-5	0.0
7	9.648623219698629E-4	1.3038680026619768E-5	2.6077360053239537E-5	1.1734812023957793E-4	2.6077360053239537E-5	0.0
8	9.648623219698629E-4	2.6077360053239537E-5	3.911604007985931E-5	1.303868002661977E-4	3.911604007985931E-5	0.0
9	9.648623219698629E-4	3.911604007985931E-5	5.2154720106479074E-5	1.303868002661977E-4	5.2154720106479074E-5	0.0
10-14	9.779010019964826E-4	3.911604007985931E-5	5.2154720106479074E-5	1.4342548029281748E-4	6.258566412777491E-5	0.0
15-19	9.909396820231024E-4	4.433151209050721E-5	5.2154720106479074E-5	1.9558020039929652E-4	6.519340013309885E-5	0.0
20-24	9.961551540337503E-4	5.2154720106479074E-5	7.040887214374674E-5	2.1122661643124024E-4	6.519340013309885E-5	0.0
25-29	0.0010430944021295816	5.737019211712699E-5	9.909396820231024E-5	2.6598907254304335E-4	7.562434415439467E-5	0.0
30-34	0.0011135032742733285	7.823208015971862E-5	1.1734812023957793E-4	3.3118247267614217E-4	1.0170170420763418E-4	0.0
35-39	0.0013586304587737798	9.387849619166231E-5	1.4342548029281748E-4	3.9116040079859303E-4	1.4603321629814143E-4	5.2154720106479076E-6
40-44	0.0015959344352582596	1.0430944021295815E-4	1.5646416031943724E-4	4.3809964889442426E-4	1.903647283886486E-4	1.3038680026619768E-5
45-49	0.0017237134995191337	1.0430944021295815E-4	1.9036472838864865E-4	4.902543690009034E-4	2.4251944849512774E-4	2.6077360053239537E-5
50-54	0.0018332384117427394	1.1734812023957793E-4	2.2165756045253605E-4	5.684864491606219E-4	2.6859680854836727E-4	2.6077360053239537E-5
55-59	0.00193233237994505	1.1995585624490188E-4	2.920664325962828E-4	6.049947532351573E-4	3.024973766175787E-4	3.911604007985931E-5
60-64	0.002036641820158008	1.356022722768456E-4	3.181437926495223E-4	6.362875852990446E-4	3.2857473667081814E-4	6.519340013309885E-5
65-69	0.002112266164312403	1.5385642431411326E-4	3.6247530474002956E-4	6.571494733416363E-4	3.4943662471340975E-4	8.605528817569047E-5
70-74	0.005077262002365739	2.0079567240994443E-4	3.9898360881456495E-4	7.014809854321436E-4	3.9898360881456495E-4	9.127076018633838E-5
75-79	0.026804918398724918	2.3469624047915585E-4	4.3549191288910023E-4	7.35381553501355E-4	4.5374606492636793E-4	9.127076018633838E-5
80-84	0.031728323976776544	2.7381228055901516E-4	4.6417700894766376E-4	7.562434415439466E-4	6.649726813576082E-4	9.127076018633838E-5
85-89	0.03179873284892029	3.442211527027619E-4	4.902543690009033E-4	7.718898575758903E-4	8.99668921836764E-4	9.127076018633838E-5
90-94	0.031843064361010795	3.598675687347056E-4	5.05900785032847E-4	8.266523136876934E-4	8.99668921836764E-4	9.127076018633838E-5
95-99	0.03187957266508533	3.755139847666493E-4	5.476245611180303E-4	8.840225058048204E-4	8.99668921836764E-4	9.127076018633838E-5
100-104	0.03195519700923973	4.041990808252128E-4	5.841328651925656E-4	9.153153378687077E-4	9.153153378687077E-4	9.127076018633838E-5
105-109	0.031989097577308945	4.1202228884118465E-4	6.023870172298332E-4	9.544313779485671E-4	9.257462818900036E-4	9.127076018633838E-5
110-114	0.03200474399334089	4.172377608518326E-4	6.128179612511291E-4	0.0010039783620497222	9.283540178953275E-4	9.127076018633838E-5
115-119	0.0320177826733675	4.172377608518326E-4	6.258566412777489E-4	0.0010430944021295814	9.518236419432431E-4	9.127076018633838E-5
120-124	0.032158600417654996	4.3809964889442426E-4	6.284643772830729E-4	0.0010796027062041171	9.622545859645389E-4	9.127076018633838E-5
125-129	0.03237765024210221	4.433151209050722E-4	6.415030573096927E-4	0.0011265419542999477	0.00100919383406037	9.127076018633838E-5
130-134	0.03265407025866655	4.485305929157201E-4	6.80619097389552E-4	0.0011421883703318916	0.0010561330821562014	9.387849619166234E-5
135-139	0.03287833555512441	4.5635380093169195E-4	7.145196654587633E-4	0.001173481202395779	0.001113503274273328	1.0430944021295815E-4
140-144	0.03310260085158227	4.5635380093169195E-4	7.327738174960311E-4	0.0011969508264436947	0.0011734812023957795	1.0430944021295815E-4
145-149	0.03319387161176861	4.720002169636356E-4	7.614589135545946E-4	0.0012125972424756385	0.001191735354433047	1.0430944021295815E-4
150-154	0.033264280483912355	4.928621050062273E-4	7.771053295865382E-4	0.0012673596985874414	0.0012178127144862865	1.1734812023957793E-4
155-159	0.03338423634015726	4.980775770168751E-4	7.979672176291298E-4	0.0013169066826885967	0.001262144226576794	1.1734812023957793E-4
160-164	0.033532877292460725	5.241549370701147E-4	8.083981616504256E-4	0.0013638459307844276	0.0012882215866300332	1.1734812023957793E-4
165	0.03367891050875886	5.345858810914105E-4	8.083981616504257E-4	0.0013821000828216955	0.0013169066826885967	1.1734812023957793E-4
>>END_MODULE
