##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293546
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7918394
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-177
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.130015253092985	41.0	40.0	41.0	38.0	41.0
2	40.24720442049234	41.0	40.0	41.0	38.0	41.0
3	40.29507789079452	41.0	40.0	41.0	39.0	41.0
4	40.30393764190062	41.0	40.0	41.0	39.0	41.0
5	40.31689759312305	41.0	40.0	41.0	39.0	41.0
6	40.341601971308826	41.0	40.0	41.0	39.0	41.0
7	40.31890848068434	41.0	40.0	41.0	39.0	41.0
8	40.3242399910891	41.0	40.0	41.0	39.0	41.0
9	40.27155127668565	41.0	40.0	41.0	39.0	41.0
10-14	40.25467651647544	41.0	40.0	41.0	39.0	41.0
15-19	40.18154496985121	41.0	40.0	41.0	38.0	41.0
20-24	40.06182357180004	41.0	40.0	41.0	38.0	41.0
25-29	39.891267875783896	41.0	40.0	41.0	37.8	41.0
30-34	39.631708904608686	40.8	39.4	41.0	36.6	41.0
35-39	39.268088554320485	40.0	38.6	41.0	35.2	41.0
40-44	38.88136649931791	40.0	37.4	41.0	35.0	41.0
45-49	38.42795915434367	39.4	36.2	41.0	35.0	41.0
50-54	37.844817951721026	38.4	35.0	40.8	35.0	41.0
55-59	37.09571473710452	36.6	35.0	39.4	35.0	41.0
60-64	36.16076871143315	35.6	34.8	38.0	33.8	40.0
65-69	35.797085898984065	35.0	35.0	36.6	34.0	39.0
70-74	35.4368657331272	35.0	35.0	36.0	34.0	37.6
75-79	35.21515832629698	35.0	35.0	35.4	34.0	36.8
80-84	35.12795248632487	35.0	35.0	35.0	34.0	36.0
85-89	35.69383945784966	35.8	35.6	35.8	34.4	36.6
90-94	40.13272546565795	41.0	40.0	41.0	38.0	41.0
95-99	40.12298483165641	41.0	40.0	41.0	38.0	41.0
100-104	40.02531070957268	41.0	40.0	41.0	38.0	41.0
105-109	39.90945436131776	41.0	40.0	41.0	37.8	41.0
110-114	39.75143935113424	41.0	40.0	41.0	37.0	41.0
115-119	39.53246077309791	40.8	39.0	41.0	36.0	41.0
120-124	39.240675193645856	40.0	38.4	41.0	35.0	41.0
125-129	38.36506550940024	39.2	37.2	40.4	34.6	40.8
130-134	38.63141008545244	40.0	36.8	41.0	35.0	41.0
135-139	38.306989126527625	39.2	35.6	41.0	35.0	41.0
140-144	37.65034853347203	37.8	35.0	40.8	35.0	41.0
145-149	36.90278225060693	36.4	35.0	39.2	35.0	41.0
150-154	36.177502284568	35.2	35.0	37.4	34.0	39.8
155-159	35.584730264520964	35.0	35.0	36.4	34.0	38.2
160-164	35.163096664859594	35.0	35.0	35.8	34.0	36.8
165-169	34.921536103410496	35.0	35.0	35.0	34.0	36.0
170-174	34.79752216883819	35.0	35.0	35.0	34.0	36.0
175-177	34.61400459548934	35.0	34.666666666666664	35.0	33.666666666666664	35.666666666666664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	25.0
30	303.0
31	2208.0
32	10843.0
33	39249.0
34	109303.0
35	280228.0
36	827748.0
37	2134907.0
38	3273471.0
39	1217650.0
40	22457.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.47854804901095	28.2129684377918	28.308821207936862	20.999662305260387
2	20.239003009953784	27.43075174082017	30.10298552964149	22.227259719584552
3	20.999902252906335	26.586742210604825	30.411166708804842	22.002188827683998
4	21.8587632795236	27.460303187742362	28.48634457946902	22.19458895326502
5	21.98217214248243	27.66156874740004	28.193835770233207	22.162423339884324
6	21.975554638983613	27.95001107547818	27.873013138775367	22.20142114676284
7	22.156108928148814	28.152501631012548	27.671545517942146	22.019843922896488
8	22.343078659637293	28.098955419495418	27.60285734708326	21.955108573784027
9	22.059953066240453	28.11315021707685	27.717501806553198	22.109394910129502
10-14	22.168328577739373	27.961614438483355	27.76470834868788	22.10534863508939
15-19	22.14164892527449	27.915877891400704	27.789955892571143	22.152517290753657
20-24	22.182386984027318	27.740748944798653	27.81932801020005	22.257536060973983
25-29	22.232735577441588	27.826251131226865	27.65754267847748	22.28347061285407
30-34	22.111966138588205	27.85845463107797	27.768143388671994	22.261435841661832
35-39	22.098147679946212	27.840400465043796	27.798306070650185	22.263145784359807
40-44	22.164340395287226	27.883184393198924	27.713281253749184	22.23919395776467
45-49	22.23512242507761	27.773758163587214	27.680981774839697	22.31013763649548
50-54	22.283940405087193	27.795608553956775	27.676028750274362	22.24442229068167
55-59	22.248817121249587	27.77298780535548	27.698114036760487	22.28008103663445
60-64	22.208074010967373	27.83217152367008	27.688149895041846	22.2716045703207
65-69	22.210695754719957	27.76014934341484	27.73485128423769	22.294303617627513
70-74	22.202684029109943	27.871717421487237	27.69740177111672	22.2281967782861
75-79	22.156831296851358	27.792236658090015	27.71833783466698	22.33259421039165
80-84	22.203984797927458	27.827175561104937	27.68077213637008	22.28806750459752
85-89	21.986188108346212	27.78695023258504	27.865112546811893	22.361749112256852
90-94	21.43166053310146	27.52746388220463	29.088815780582866	21.952059804111045
95-99	22.02744449287921	28.15593383980165	27.767890756577113	22.04873091074203
100-104	22.058155151207888	27.959447793389515	27.859637491391076	22.122759564011524
105-109	22.027599308938537	27.992954973485556	27.883820014274807	22.095625703301096
110-114	22.07950798872842	27.799882140687295	27.81603294988265	22.30457692070163
115-119	22.179838606144532	27.847303815511427	27.68635263487072	22.28650494347332
120-124	22.02563039905246	27.895791620936482	27.832174325956704	22.246403654054355
125-129	22.05270313587064	27.89858975837048	27.77573062190389	22.272976483854993
130-134	22.200681378533773	27.801004593601984	27.758846329080512	22.239467698783734
135-139	22.213417215055035	27.849878299438945	27.73909523719341	22.19760924831261
140-144	22.179459417155652	27.873680295749914	27.74756759353454	22.199292693559894
145-149	22.19802833388633	27.8451389030082	27.750614928996576	22.206217834108898
150-154	22.120695516648382	27.96749961919437	27.782654358734355	22.129150505422892
155-159	22.05163936540621	27.937305185235513	27.849977989182488	22.161077460175786
160-164	22.0741525260865	28.00536075102568	27.8550463241315	22.065440398756316
165-169	21.99056348172382	28.02494981469247	27.900964318533788	22.08352238504992
170-174	21.973123671332857	28.113753607155896	27.863690273135305	22.049432448375942
175-177	21.92991169245889	28.083249967584273	27.925263775605213	22.061574564351623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	1.0
11	4.5
12	9.0
13	25.0
14	68.5
15	137.5
16	278.0
17	571.5
18	1074.5
19	2039.0
20	3642.0
21	6145.0
22	9731.5
23	14633.0
24	21314.0
25	29977.0
26	40578.0
27	53313.0
28	67989.0
29	84432.0
30	102175.5
31	121339.0
32	142390.5
33	164448.0
34	187003.0
35	210983.0
36	235355.5
37	258817.5
38	282695.5
39	304542.5
40	323750.0
41	339611.5
42	350182.5
43	356248.5
44	356045.5
45	349246.5
46	337271.0
47	320540.5
48	300316.5
49	277544.5
50	252848.5
51	228724.5
52	206229.0
53	184747.0
54	164978.5
55	147980.0
56	133700.5
57	121528.0
58	110672.5
59	100956.5
60	92038.5
61	83789.5
62	75503.0
63	66973.5
64	58883.0
65	50607.5
66	42501.5
67	34955.5
68	28102.5
69	21857.5
70	16585.5
71	12421.5
72	8933.0
73	6136.0
74	4041.5
75	2638.0
76	1660.0
77	999.5
78	601.5
79	355.5
80	183.0
81	87.0
82	36.5
83	19.5
84	10.0
85	4.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-177	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	464.0
92-93	1403.0
94-95	1507.0
96-97	1950.0
98-99	2520.0
100-101	3331.0
102-103	3260.0
104-105	3716.0
106-107	4190.0
108-109	5218.0
110-111	4814.0
112-113	5975.0
114-115	5061.0
116-117	8437.0
118-119	6718.0
120-121	6951.0
122-123	7441.0
124-125	7837.0
126-127	7141.0
128-129	7541.0
130-131	8075.0
132-133	24488.0
134-135	59789.0
136-137	63024.0
138-139	61690.0
140-141	62433.0
142-143	60337.0
144-145	58135.0
146-147	54823.0
148-149	60498.0
150-151	64425.0
152-153	59785.0
154-155	61121.0
156-157	57548.0
158-159	59261.0
160-161	60803.0
162-163	63064.0
164-165	64831.0
166-167	68080.0
168-169	60892.0
170-171	66794.0
172-173	61091.0
174-175	64383.0
176-177	6497549.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.79882298225354
#Duplication Level	Percentage of total
1	84.78771928886208
2	12.290246944597301
3	2.0431964304809074
4	0.47841446387596276
5	0.18335860022994974
6	0.08555832555666326
7	0.037343647526467155
8	0.030271671606623182
9	0.013655262411693911
>10	0.050235364852338864
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	6.945852909062115E-4	0.0	0.0	1.2628823471022028E-5	0.0	0.0
2	6.945852909062115E-4	1.2628823471022028E-5	1.2628823471022028E-5	1.2628823471022028E-5	0.0	0.0
3	7.072141143772336E-4	1.2628823471022028E-5	1.2628823471022028E-5	5.051529388408811E-5	0.0	0.0
4	7.072141143772336E-4	1.2628823471022028E-5	1.2628823471022028E-5	5.051529388408811E-5	0.0	0.0
5	7.198429378482556E-4	2.5257646942044056E-5	1.2628823471022028E-5	6.314411735511014E-5	0.0	0.0
6	7.198429378482556E-4	3.788647041306608E-5	1.2628823471022028E-5	6.314411735511014E-5	0.0	0.0
7	7.324717613192776E-4	3.788647041306608E-5	1.2628823471022028E-5	6.314411735511014E-5	1.2628823471022028E-5	0.0
8	7.324717613192776E-4	3.788647041306608E-5	1.2628823471022028E-5	6.314411735511014E-5	1.2628823471022028E-5	0.0
9	7.324717613192776E-4	5.051529388408811E-5	2.5257646942044056E-5	6.314411735511014E-5	1.2628823471022028E-5	0.0
10-14	7.754097611207525E-4	7.324717613192777E-5	5.809258796670133E-5	6.819564674351896E-5	1.7680352859430843E-5	0.0
15-19	7.956158786743878E-4	1.0103058776817623E-4	1.1113364654499385E-4	9.345329368556303E-5	2.5257646942044056E-5	0.0
20-24	8.006674080627966E-4	1.4144282287544672E-4	1.1871094062760707E-4	1.0103058776817623E-4	3.536070571886168E-5	0.0
25-29	8.132962315338186E-4	1.6417470512328637E-4	1.262882347102203E-4	1.0608211715658503E-4	5.809258796670133E-5	0.0
30-34	8.739145841947243E-4	1.74277763900104E-4	1.4144282287544672E-4	1.288139994044247E-4	7.072141143772336E-5	0.0
35-39	0.0010507181127890326	1.9448388145373922E-4	1.692262345116952E-4	1.7932929328851282E-4	8.840176429715421E-5	2.0206117553635245E-5
40-44	0.0012249958766891369	2.247930577841921E-4	1.818550579827172E-4	2.247930577841921E-4	1.1871094062760707E-4	5.3041058578292515E-5
45-49	0.0014699950520269639	2.399476459494185E-4	2.0206117553635245E-4	2.374218812552141E-4	1.5407164634646873E-4	7.577294082613216E-5
50-54	0.0016467985806212725	2.399476459494185E-4	2.2984458717260092E-4	2.5005070472623617E-4	2.0206117553635245E-4	1.0355635246238062E-4
55-59	0.0017402518743068356	2.6520529289146257E-4	2.778341163624846E-4	2.80359881056689E-4	2.67731057585667E-4	1.1365941123919826E-4
60-64	0.0018311794032981944	2.753083516682802E-4	3.2834941024657274E-4	3.510812924944124E-4	3.3592670432918597E-4	1.288139994044247E-4
65-69	0.0019776737555620497	2.9046293983350666E-4	3.384524690233904E-4	3.813904688248652E-4	3.4855552780020796E-4	1.6417470512328637E-4
70-74	0.0048747258598145025	2.9046293983350666E-4	3.561328218828212E-4	3.965450569900917E-4	3.813904688248652E-4	1.8185505798271723E-4
75-79	0.02520965741285417	3.0309176330452865E-4	3.662358806596388E-4	4.2685423332054453E-4	4.42008821485771E-4	2.0206117553635245E-4
80-84	0.029652477509959717	3.131948220813463E-4	3.73813174742252E-4	4.748437625104283E-4	6.390184676337147E-4	2.1468999900737447E-4
85-89	0.029667632098124945	3.157205867755507E-4	3.9149352760168286E-4	5.278848210887207E-4	7.930901139801833E-4	2.1468999900737447E-4
90-94	0.029738353509562672	3.3592670432918597E-4	4.041223510727049E-4	5.885031737496265E-4	8.057189374512054E-4	2.1468999900737447E-4
95-99	0.029748456568339486	3.536070571886168E-4	4.1422540984952254E-4	6.491215264105323E-4	8.360281137816583E-4	2.1468999900737447E-4
100-104	0.029758559627116304	3.561328218828212E-4	4.2937999801474894E-4	6.996368202946204E-4	8.688630548063155E-4	2.1468999900737447E-4
105-109	0.02978129150936415	3.9149352760168286E-4	4.3190576270895335E-4	7.501521141787084E-4	8.890691723599508E-4	2.1468999900737447E-4
110-114	0.0298116006856946	4.041223510727049E-4	4.495861155683842E-4	7.728839964265481E-4	8.96646466442564E-4	2.2731882247839651E-4
115-119	0.029826755273859827	4.1422540984952254E-4	4.6474070373361066E-4	8.15821996228023E-4	9.016979958309728E-4	2.2731882247839651E-4
120-124	0.02994294044979323	4.445345861799754E-4	4.975756447582679E-4	8.688630548063155E-4	9.496875250208564E-4	2.2984458717260092E-4
125-129	0.030172785036965834	4.723179978162238E-4	5.203075270061076E-4	9.59790583797674E-4	9.875739954339226E-4	2.5005070472623617E-4
130-134	0.030283918683510824	4.925241153698591E-4	5.531424680307648E-4	9.976770542107403E-4	0.0010153574070701711	2.525764694204406E-4
135-139	0.03046577374149354	5.001014094524723E-4	5.859774090554221E-4	0.0010279862305411932	0.0010481923480948282	2.525764694204406E-4
140-144	0.030665309152335684	5.051529388408812E-4	6.087092913032617E-4	0.0010481923480948282	0.0010810272891194855	2.525764694204406E-4
145-149	0.030794123151740117	5.177817623119032E-4	6.364927029395102E-4	0.001070924230342668	0.001121439524226756	2.525764694204406E-4
150-154	0.03087242185726045	5.177817623119032E-4	6.541730557989411E-4	0.0011189137595325517	0.0011669032887224355	2.525764694204406E-4
155-159	0.030950720562780788	5.177817623119032E-4	6.693276439641675E-4	0.0011492229358630046	0.0011845836415818663	2.525764694204406E-4
160-164	0.03113762715015191	5.405136445597428E-4	6.693276439641675E-4	0.0011871094062760707	0.0011871094062760707	2.525764694204406E-4
165-166	0.031313167796399116	5.556682327249692E-4	6.756420556996785E-4	0.0011997382297470926	0.0011871094062760707	2.525764694204406E-4
>>END_MODULE
