##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293547
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7790753
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-177
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.233503231330786	41.0	40.0	41.0	39.0	41.0
2	40.30731843250582	41.0	40.0	41.0	39.0	41.0
3	40.33179321690727	41.0	40.0	41.0	39.0	41.0
4	40.339289539791594	41.0	40.0	41.0	39.0	41.0
5	40.331199564406674	41.0	40.0	41.0	39.0	41.0
6	40.36624996325772	41.0	40.0	41.0	39.0	41.0
7	40.348269416319575	41.0	40.0	41.0	39.0	41.0
8	40.35448973931018	41.0	40.0	41.0	39.0	41.0
9	40.31766480082221	41.0	40.0	41.0	39.0	41.0
10-14	40.29313495114015	41.0	40.0	41.0	39.0	41.0
15-19	40.21711369876571	41.0	40.0	41.0	38.4	41.0
20-24	40.10501095336998	41.0	40.0	41.0	38.0	41.0
25-29	39.93393162381095	41.0	40.0	41.0	37.8	41.0
30-34	39.68842826874373	41.0	39.6	41.0	36.8	41.0
35-39	39.32279816854673	40.0	38.6	41.0	35.2	41.0
40-44	38.94704694141889	40.0	37.6	41.0	35.0	41.0
45-49	38.49236757987322	39.6	36.2	41.0	35.0	41.0
50-54	37.912800290292864	38.4	35.2	41.0	35.0	41.0
55-59	37.162935983209834	36.8	35.0	39.4	35.0	41.0
60-64	36.20813128076323	35.6	34.8	38.2	34.2	40.2
65-69	35.82290019976246	35.0	35.0	36.8	34.0	39.0
70-74	35.4320667078009	35.0	35.0	36.0	34.0	37.4
75-79	35.184030786241074	35.0	35.0	35.4	34.0	36.6
80-84	35.074819776727615	35.0	35.0	35.0	34.0	36.0
85-89	35.650938927212806	35.8	35.6	35.8	34.4	36.6
90-94	40.161680209044455	41.0	40.0	41.0	38.0	41.0
95-99	40.15201682179408	41.0	40.0	41.0	38.0	41.0
100-104	40.065186100848514	41.0	40.0	41.0	38.0	41.0
105-109	39.957023831862784	41.0	40.0	41.0	38.0	41.0
110-114	39.799588840677	41.0	40.0	41.0	37.2	41.0
115-119	39.582769006985	41.0	39.2	41.0	36.4	41.0
120-124	39.29720730032325	40.0	38.6	41.0	35.0	41.0
125-129	38.39845110620969	39.4	37.2	40.2	34.6	40.6
130-134	38.686591365571545	40.0	36.8	41.0	35.0	41.0
135-139	38.361899984906316	39.4	35.6	41.0	35.0	41.0
140-144	37.700212652319735	38.0	35.0	40.8	35.0	41.0
145-149	36.944261254103154	36.4	35.0	39.2	35.0	41.0
150-154	36.209994684910676	35.4	35.0	37.6	34.0	40.0
155-159	35.600708693887285	35.0	35.0	36.4	34.0	38.2
160-164	35.17571602548419	35.0	35.0	35.8	34.0	36.8
165-169	34.92771617867413	35.0	35.0	35.0	34.0	36.0
170-174	34.80125735806594	35.0	35.0	35.0	34.0	36.0
175-177	34.62141613098894	35.0	34.666666666666664	35.0	33.666666666666664	35.666666666666664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	23.0
30	295.0
31	2166.0
32	10524.0
33	37774.0
34	103472.0
35	263510.0
36	788237.0
37	2066694.0
38	3255098.0
39	1243461.0
40	19496.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.485246291340516	28.20831311171077	28.343062602549455	20.963377994399256
2	20.26267550774617	27.424152710270754	30.097514322428143	22.215657459554937
3	20.983799640419868	26.605284495606522	30.375972643465914	22.034943220507696
4	21.83299868446606	27.490436418661968	28.484833237557396	22.191731659314577
5	21.96315298405687	27.64297623092402	28.217760208801383	22.176110576217727
6	21.987104455756715	27.945925124310833	27.878152471269463	22.188817948662987
7	22.141903356453476	28.142209103535947	27.71832196451357	21.997565575497003
8	22.328727402858235	28.122711630056813	27.596716260931387	21.951844706153565
9	22.045404340247984	28.145969972350553	27.697605096708884	22.111020590692583
10-14	22.16626557150509	27.988365181132043	27.773045814698527	22.07232343266434
15-19	22.11076130895178	27.94156867763617	27.81327042456615	22.134399588845906
20-24	22.182138234904894	27.762507680579784	27.81978327383759	22.235570810677736
25-29	22.223384568860034	27.84342925516956	27.65997330424928	22.273212871721128
30-34	22.100908602801294	27.87018148309926	27.788253587297657	22.240656326801787
35-39	22.094957958492586	27.843344539353254	27.80875738198862	22.252940120165533
40-44	22.16131611411631	27.88542262859572	27.739439307086233	22.21382195020173
45-49	22.24189625829493	27.775507707663174	27.682145743806792	22.3004502902351
50-54	22.263134256727174	27.801633551981432	27.70051880736047	22.234713383930924
55-59	22.227232720636888	27.781037340036324	27.6979182885146	22.293811650812188
60-64	22.18586573082217	27.86735248826397	27.69634462804815	22.25043715286571
65-69	22.19407546356559	27.7609057815079	27.747659308413446	22.297359446513067
70-74	22.190383907691594	27.87925120973544	27.69478123616549	22.235583646407477
75-79	22.14130264430152	27.810307938141538	27.72602596950513	22.322363448051814
80-84	22.208112617612187	27.837031927465805	27.693264052909903	22.261591402012105
85-89	21.962793583624073	27.8091527224647	27.875159179093473	22.35289451481776
90-94	21.39653843737604	27.53333233892505	29.130759392209782	21.93936983148913
95-99	22.020827851424244	28.155072244338207	27.788894201177172	22.035205703060377
100-104	22.05183115832067	27.96891753898371	27.85745931539657	22.121791987299048
105-109	22.02340443771092	28.002899389815006	27.880981766693075	22.092714405780995
110-114	22.052067633774314	27.80457967698436	27.848737534253377	22.294615154987948
115-119	22.16475277581003	27.851736239822948	27.710187358809986	22.273323625557037
120-124	22.01690123317316	27.902931710091693	27.83628173283636	22.243885323898784
125-129	22.050596871408075	27.902329323607177	27.767743236276328	22.279330568708417
130-134	22.188198539331736	27.81455626823568	27.75832341814551	22.23892177428708
135-139	22.18686846405568	27.860395592025355	27.75886870825024	22.193867235668726
140-144	22.177447434474505	27.864896397008433	27.75753568680564	22.200120481711423
145-149	22.18460074188094	27.858369848753657	27.763031320004995	22.19399808936041
150-154	22.114587935201595	27.972891685763514	27.80406480748351	22.10845557155138
155-159	22.04576527767698	27.93660381682442	27.841128756148976	22.176502149349623
160-164	22.068903049788812	28.022018396544308	27.848113590468156	22.06096496319872
165-169	21.972111755552586	28.027031534036173	27.915679613190726	22.08517709722052
170-174	21.952161623253705	28.11849721511404	27.90092511146907	22.028416050163184
175-177	21.919355478372545	28.116836614089575	27.924391221451085	22.039416686086792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	6.0
12	11.0
13	22.5
14	63.5
15	138.0
16	284.5
17	546.5
18	1090.5
19	2054.0
20	3625.5
21	6142.0
22	9731.0
23	14658.5
24	21317.0
25	29696.0
26	40059.5
27	52859.5
28	67345.0
29	83498.0
30	101246.5
31	119998.5
32	140602.5
33	162400.0
34	184805.5
35	208449.0
36	232046.0
37	255161.5
38	278346.0
39	300486.5
40	319135.5
41	333614.5
42	344219.5
43	349649.5
44	349242.0
45	343116.0
46	331749.0
47	315368.5
48	294862.0
49	272258.0
50	248698.5
51	225064.0
52	202180.5
53	181214.0
54	162341.5
55	145577.5
56	131365.0
57	119012.0
58	108541.5
59	99252.0
60	90348.0
61	82115.0
62	74081.0
63	65697.0
64	57576.5
65	49651.5
66	41628.0
67	34318.5
68	27538.5
69	21374.5
70	16327.5
71	12193.5
72	8721.0
73	5883.0
74	3938.5
75	2648.0
76	1661.0
77	952.5
78	564.5
79	345.5
80	180.5
81	82.5
82	49.0
83	28.0
84	11.5
85	5.0
86	2.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-177	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	432.0
92-93	841.0
94-95	1383.0
96-97	2174.0
98-99	2110.0
100-101	2523.0
102-103	2780.0
104-105	3037.0
106-107	3286.0
108-109	3714.0
110-111	4026.0
112-113	4164.0
114-115	4333.0
116-117	4558.0
118-119	5056.0
120-121	6718.0
122-123	5456.0
124-125	5797.0
126-127	5999.0
128-129	6468.0
130-131	6500.0
132-133	20605.0
134-135	50662.0
136-137	58868.0
138-139	62636.0
140-141	52696.0
142-143	50961.0
144-145	49517.0
146-147	46342.0
148-149	47718.0
150-151	45607.0
152-153	45442.0
154-155	45986.0
156-157	48226.0
158-159	50442.0
160-161	49144.0
162-163	51020.0
164-165	55047.0
166-167	53763.0
168-169	57660.0
170-171	58509.0
172-173	56070.0
174-175	56248.0
176-177	6596229.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.88953768339366
#Duplication Level	Percentage of total
1	85.00816973680874
2	11.999629714264788
3	2.0647873312852605
4	0.5029783108337397
5	0.18649947748336515
6	0.08353512144616762
7	0.04255393644148568
8	0.022979696900160038
9	0.0220749932354072
>10	0.06679168130089255
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.262649194500198E-4	1.283572974268341E-5	0.0	0.0	1.283572974268341E-5	0.0
2	5.262649194500198E-4	1.283572974268341E-5	0.0	1.283572974268341E-5	2.567145948536682E-5	0.0
3	5.391006491927032E-4	3.850718922805023E-5	1.283572974268341E-5	2.567145948536682E-5	2.567145948536682E-5	0.0
4	5.519363789353866E-4	3.850718922805023E-5	1.283572974268341E-5	2.567145948536682E-5	2.567145948536682E-5	0.0
5	5.519363789353866E-4	3.850718922805023E-5	2.567145948536682E-5	2.567145948536682E-5	2.567145948536682E-5	0.0
6	5.519363789353866E-4	3.850718922805023E-5	2.567145948536682E-5	2.567145948536682E-5	3.850718922805023E-5	0.0
7	5.6477210867807E-4	3.850718922805023E-5	3.850718922805023E-5	3.850718922805023E-5	5.134291897073364E-5	0.0
8	5.776078384207535E-4	3.850718922805023E-5	5.134291897073364E-5	5.134291897073364E-5	5.134291897073364E-5	0.0
9	5.776078384207535E-4	3.850718922805023E-5	5.134291897073364E-5	5.134291897073364E-5	6.417864871341704E-5	0.0
10-14	5.955778600605102E-4	3.850718922805023E-5	5.647721086780701E-5	6.161150276488036E-5	6.931294061049042E-5	0.0
15-19	6.084135898031936E-4	5.1342918970733634E-5	7.18800865590271E-5	1.2579015147829743E-4	8.214867035317381E-5	0.0
20-24	6.392193411856337E-4	8.214867035317382E-5	8.985010819878386E-5	1.69431632603421E-4	1.1295442173561399E-4	0.0
25-29	6.443536330827071E-4	1.411930271695175E-4	1.001186919929306E-4	2.0793882183147124E-4	1.3862588122098078E-4	0.0
30-34	7.136665736931976E-4	1.411930271695175E-4	1.1808871363268737E-4	2.413117191624481E-4	1.4376017311805417E-4	0.0
35-39	8.779639143995452E-4	1.5402875691220092E-4	1.4889446501512753E-4	2.669831786478149E-4	1.822673623461044E-4	1.0268583794146728E-5
40-44	0.0010756341524368698	1.5402875691220092E-4	1.6686448665488433E-4	3.080575138244018E-4	2.0537167588293454E-4	2.0537167588293456E-5
45-49	0.001227095763400534	1.5402875691220092E-4	1.719987785519577E-4	3.645347246922088E-4	2.4387886511098477E-4	5.1342918970733634E-5
50-54	0.001301542995908098	1.6686448665488433E-4	2.0280452993439788E-4	4.133104977144058E-4	3.029232219273285E-4	5.647721086780701E-5
55-59	0.0013734230824671248	1.6686448665488433E-4	2.3104313536830137E-4	4.6978770858221276E-4	3.1319180572147515E-4	1.0268583794146727E-4
60-64	0.0014838103582542022	1.69431632603421E-4	2.3874457321391144E-4	5.00593459964653E-4	3.362961192583053E-4	1.0525298389000396E-4
65-69	0.0015710933205044496	1.951030920887878E-4	2.5671459485366823E-4	5.134291897073364E-4	3.5683328684659875E-4	1.360587352724441E-4
70-74	0.004631131291160174	2.0537167588293454E-4	2.695503245963516E-4	5.750406924722167E-4	4.36414811251236E-4	1.5402875691220092E-4
75-79	0.025363401971542417	2.0537167588293454E-4	2.772517624419616E-4	6.392193411856338E-4	5.134291897073365E-4	1.6686448665488433E-4
80-84	0.029953458927526006	2.490131570080581E-4	2.849532002875717E-4	6.85427968259294E-4	6.931294061049041E-4	1.7970021639756773E-4
85-89	0.03000993613839381	2.695503245963516E-4	3.0805751382440184E-4	6.956965520534408E-4	8.72829622502472E-4	1.7970021639756773E-4
90-94	0.030061279057364543	2.849532002875717E-4	3.208932435670852E-4	7.419051791271011E-4	8.72829622502472E-4	1.7970021639756773E-4
95-99	0.03013315914392357	2.952217840817184E-4	3.260275354641586E-4	7.881138062007613E-4	8.72829622502472E-4	1.7970021639756773E-4
100-104	0.03016396489530601	3.054903678758652E-4	3.4913184900098874E-4	8.163524116346649E-4	8.753967684510085E-4	1.7970021639756773E-4
105-109	0.030212740668328207	3.260275354641586E-4	3.8507189228050227E-4	8.31755287325885E-4	9.010682279363754E-4	1.7970021639756773E-4
110-114	0.03025894929540187	3.362961192583053E-4	4.004747679717224E-4	8.599938927597884E-4	9.190382495761322E-4	1.7970021639756773E-4
115-119	0.03029488933868138	3.4656470305245203E-4	4.2871337340562586E-4	8.753967684510085E-4	9.318739793188155E-4	1.7970021639756773E-4
120-124	0.030341097965755043	3.4656470305245203E-4	4.543848328909928E-4	8.933667900907653E-4	9.960526280322324E-4	1.7970021639756773E-4
125-129	0.030510529598358465	3.696690165892822E-4	4.903248761705063E-4	9.164711036275955E-4	0.0010422612551058928	1.9253594614025113E-4
130-134	0.030726169858035546	3.8507189228050227E-4	5.313992113470933E-4	9.472768550100357E-4	0.0010756341524368698	1.9253594614025113E-4
135-139	0.030844258571668233	3.876390382290389E-4	5.622049627295332E-4	9.575454388041823E-4	0.0011141413416649198	1.9253594614025113E-4
140-144	0.031016257350220188	3.979076220231857E-4	5.6477210867807E-4	9.806497523410125E-4	0.0011500813849444334	2.1307311372854458E-4
145-149	0.031134346063852875	4.004747679717224E-4	5.878764222149002E-4	0.0010140226496719894	0.0011603499687385802	2.18207405625618E-4
150-154	0.031229330463948733	4.2871337340562586E-4	5.930107141119735E-4	0.0010627984226941863	0.001227095763400534	2.18207405625618E-4
155-159	0.03134998632352996	4.36414811251236E-4	6.340850492885604E-4	0.001080768444333943	0.0012707372445256574	2.2590884347122804E-4
160-164	0.03161440235622923	4.36414811251236E-4	6.70025092568074E-4	0.0011064399038193098	0.0013349158932390744	2.3104313536830137E-4
165-166	0.03186469908621156	4.3641481125123593E-4	6.802936763622207E-4	0.0011167084876134567	0.0013734230824671248	2.3104313536830137E-4
>>END_MODULE
