##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293548
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6058032
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-177
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.11756441695917	41.0	40.0	41.0	38.0	41.0
2	40.236093008422536	41.0	40.0	41.0	38.0	41.0
3	40.28161719845653	41.0	40.0	41.0	39.0	41.0
4	40.29499381977514	41.0	40.0	41.0	39.0	41.0
5	40.300748328830224	41.0	40.0	41.0	39.0	41.0
6	40.319453908464006	41.0	40.0	41.0	39.0	41.0
7	40.30909427351985	41.0	40.0	41.0	39.0	41.0
8	40.31604752170342	41.0	40.0	41.0	39.0	41.0
9	40.26655240513751	41.0	40.0	41.0	39.0	41.0
10-14	40.23542038074412	41.0	40.0	41.0	38.4	41.0
15-19	40.16275572661221	41.0	40.0	41.0	38.0	41.0
20-24	40.0337092639986	41.0	40.0	41.0	38.0	41.0
25-29	39.86757151497384	41.0	40.0	41.0	37.6	41.0
30-34	39.61982511812417	40.8	39.2	41.0	36.6	41.0
35-39	39.25138952715997	40.0	38.6	41.0	35.2	41.0
40-44	38.87011455205255	40.0	37.4	41.0	35.0	41.0
45-49	38.42689533498667	39.4	36.2	41.0	35.0	41.0
50-54	37.85719454766829	38.4	35.0	40.8	35.0	41.0
55-59	37.113616072018104	36.6	35.0	39.4	35.0	41.0
60-64	36.176301115609824	35.6	34.8	38.2	33.6	40.2
65-69	35.82249743811192	35.0	35.0	36.8	34.0	39.0
70-74	35.45014420524685	35.0	35.0	36.0	34.0	37.6
75-79	35.21138141891625	35.0	35.0	35.4	34.0	36.8
80-84	35.10288403890901	35.0	35.0	35.0	34.0	36.0
85-89	35.65627857363579	35.8	35.6	35.8	34.4	36.6
90-94	40.08790445809615	41.0	40.0	41.0	38.0	41.0
95-99	40.07098137257741	41.0	40.0	41.0	38.0	41.0
100-104	39.9792625135347	41.0	40.0	41.0	38.0	41.0
105-109	39.8596504018871	41.0	40.0	41.0	37.6	41.0
110-114	39.69933316501869	41.0	39.8	41.0	36.8	41.0
115-119	39.46353838475534	40.6	39.0	41.0	35.6	41.0
120-124	39.17463051269018	40.0	38.2	41.0	35.0	41.0
125-129	38.282715772695994	39.2	37.2	40.2	34.2	40.6
130-134	38.58368237904006	40.0	36.6	41.0	35.0	41.0
135-139	38.264613252749584	39.2	35.6	41.0	35.0	41.0
140-144	37.61424774222762	37.6	35.0	40.6	35.0	41.0
145-149	36.86024471597274	36.4	35.0	39.2	34.4	41.0
150-154	36.15267992330122	35.2	35.0	37.4	34.0	39.8
155-159	35.55615742093322	35.0	35.0	36.2	34.0	38.2
160-164	35.136233502362195	35.0	35.0	35.6	34.0	36.8
165-169	34.896006950041915	35.0	35.0	35.0	34.0	36.0
170-174	34.764234661475406	35.0	35.0	35.0	34.0	36.0
175-177	34.57987197739823	35.0	34.666666666666664	35.0	33.666666666666664	35.666666666666664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	35.0
30	295.0
31	1967.0
32	9453.0
33	32659.0
34	89655.0
35	227028.0
36	652822.0
37	1646300.0
38	2476760.0
39	907084.0
40	13973.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.47982513132978	28.244073322821666	28.32083092330975	20.955270622538805
2	20.2739602564001	27.441403412857508	30.07432116568549	22.210315165056905
3	20.98229259931278	26.592480858470207	30.376300422315367	22.048926119901644
4	21.864641850686827	27.498484656403267	28.51038092898816	22.126492563921747
5	21.968735061155172	27.696700842781947	28.18402081732153	22.150543278741345
6	21.942257815739502	27.949357150969163	27.937075934891066	22.171309098400272
7	22.120550040012997	28.166804004997005	27.70615936000338	22.006486594986622
8	22.292800698312586	28.135688289530332	27.63006203994961	21.941448972207475
9	22.04542663359982	28.116936325196036	27.755961011760917	22.081676029443226
10-14	22.141946427486683	27.988561301756082	27.782319406698413	22.087172864058825
15-19	22.112738922475152	27.950126377675126	27.81400296333859	22.12313173651113
20-24	22.16768415881593	27.761903535669667	27.83179752104314	22.23861478447126
25-29	22.21887570088768	27.838284776310196	27.68566755672469	22.257171966077433
30-34	22.09650262659557	27.877379980825456	27.789166514802165	22.23695087777681
35-39	22.08625837565731	27.841384792949263	27.814960369968333	22.257396461425095
40-44	22.139866544118618	27.89378794961796	27.739153573305654	22.227191932957766
45-49	22.243022156370255	27.773217440911502	27.68502048189907	22.298739920819173
50-54	22.256960676338455	27.81812311324866	27.70438650703727	22.220529703375618
55-59	22.241747815132044	27.798255274980388	27.691732232513793	22.268264677373775
60-64	22.178836295351363	27.88463646279848	27.68134602128216	22.255181220567998
65-69	22.18343184717413	27.778073803505826	27.74886299709213	22.289631352227918
70-74	22.19568335063268	27.891704764847724	27.685126126768562	22.22748575775103
75-79	22.14038816566172	27.8102327620587	27.730011990692688	22.31936708158689
80-84	22.178440127090777	27.8584266309587	27.68526808706194	22.277865154888584
85-89	21.9686624303074	27.814095402599392	27.861411098521767	22.355831068571444
90-94	21.405968739888667	27.56067129339206	29.09704237349692	21.936317593222356
95-99	21.993554423770263	28.175337484054435	27.8082712095426	22.022836882632703
100-104	22.030002696309943	27.975736384196793	27.868297226714972	22.125963692778292
105-109	22.00932067455816	28.006108527514606	27.897748296374797	22.086822501552437
110-114	22.04885382058424	27.83661028242398	27.830338429766105	22.284197467225674
115-119	22.16675714902815	27.87470134095557	27.691787154002107	22.266754356014168
120-124	22.0091943566252	27.915030943750587	27.829629936072653	22.24614476355156
125-129	22.02583278044541	27.929183196120626	27.772501286343054	22.272482737090915
130-134	22.17917991168902	27.822285384585683	27.752085133345144	22.246449570380157
135-139	22.193771221283946	27.858450221027642	27.755360778532555	22.192417779155853
140-144	22.16985093446719	27.89857403888764	27.740841562478046	22.190733464167124
145-149	22.18348715232525	27.853030566270455	27.764160734148387	22.199321547255916
150-154	22.080234534352606	27.997055393547893	27.81709713482951	22.105612937269992
155-159	22.03209255095514	27.95941916833362	27.846637039450606	22.161851241260635
160-164	22.042507857833655	28.034589579303677	27.886254080029016	22.036648482833655
165-169	21.956567688642828	28.065335135986036	27.916999505599353	22.061097669771783
170-174	21.92812540040642	28.15324291320886	27.899626004846027	22.019005681538694
175-177	21.899799975910124	28.140566392222944	27.93768087186238	22.021952760004552
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	3.0
12	7.0
13	18.0
14	38.5
15	90.0
16	209.0
17	429.0
18	854.5
19	1610.5
20	2807.5
21	4655.0
22	7396.5
23	11291.5
24	16478.0
25	23018.0
26	31024.0
27	40949.5
28	52074.0
29	64457.0
30	78639.5
31	93594.5
32	109336.5
33	126147.5
34	143843.0
35	161907.0
36	180218.0
37	198716.0
38	217103.5
39	234174.5
40	248586.0
41	260300.5
42	268685.5
43	272517.5
44	272023.5
45	267195.0
46	258571.5
47	245713.5
48	229168.0
49	211958.0
50	193627.0
51	174542.0
52	156777.5
53	140410.0
54	125692.5
55	113108.5
56	102262.0
57	92881.0
58	84477.5
59	76850.0
60	70237.0
61	63772.0
62	57325.5
63	50881.5
64	44372.0
65	38249.5
66	32111.0
67	26167.0
68	21070.0
69	16457.5
70	12470.5
71	9319.5
72	6652.5
73	4596.5
74	3057.0
75	2016.0
76	1301.0
77	800.5
78	459.5
79	257.0
80	146.0
81	75.5
82	32.5
83	12.5
84	6.5
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-177	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	385.0
92-93	749.0
94-95	1171.0
96-97	1708.0
98-99	1958.0
100-101	2211.0
102-103	2593.0
104-105	3046.0
106-107	3485.0
108-109	3553.0
110-111	3858.0
112-113	3939.0
114-115	4300.0
116-117	5934.0
118-119	5770.0
120-121	9867.0
122-123	5590.0
124-125	5160.0
126-127	6669.0
128-129	5928.0
130-131	6201.0
132-133	18480.0
134-135	47754.0
136-137	52035.0
138-139	43247.0
140-141	49719.0
142-143	41803.0
144-145	43274.0
146-147	81996.0
148-149	46408.0
150-151	40226.0
152-153	45459.0
154-155	37936.0
156-157	49301.0
158-159	41747.0
160-161	39556.0
162-163	42994.0
164-165	42598.0
166-167	52444.0
168-169	46363.0
170-171	48210.0
172-173	49434.0
174-175	50654.0
176-177	4962319.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.46631873322649
#Duplication Level	Percentage of total
1	87.77690749401552
2	10.116737190225509
3	1.5170304875950715
4	0.3409371267190394
5	0.10632700372891879
6	0.04683657074905827
7	0.03672795805195254
8	0.013753657199600824
9	0.017065965859985655
>10	0.027676545855329013
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.263084777366643E-4	0.0	0.0	0.0	0.0	0.0
2	7.263084777366643E-4	3.301402171530292E-5	0.0	0.0	0.0	0.0
3	7.428154885943158E-4	4.9521032572954387E-5	1.650701085765146E-5	1.650701085765146E-5	0.0	0.0
4	7.428154885943158E-4	4.9521032572954387E-5	1.650701085765146E-5	1.650701085765146E-5	0.0	0.0
5	7.593224994519672E-4	6.602804343060584E-5	3.301402171530292E-5	1.650701085765146E-5	0.0	0.0
6	7.593224994519672E-4	6.602804343060584E-5	3.301402171530292E-5	1.650701085765146E-5	1.650701085765146E-5	0.0
7	7.758295103096187E-4	6.602804343060584E-5	3.301402171530292E-5	1.650701085765146E-5	1.650701085765146E-5	0.0
8	7.758295103096187E-4	6.602804343060584E-5	3.301402171530292E-5	1.650701085765146E-5	3.301402171530292E-5	0.0
9	7.923365211672702E-4	9.904206514590877E-5	4.9521032572954387E-5	4.9521032572954387E-5	3.301402171530292E-5	0.0
10-14	8.35254749397164E-4	1.1554907600356023E-4	5.612383691601496E-5	6.272664125907555E-5	4.6219630401424095E-5	0.0
15-19	8.418575537402246E-4	1.1554907600356023E-4	6.602804343060584E-5	1.1224767383202993E-4	4.9521032572954387E-5	0.0
20-24	8.616659667694063E-4	1.1554907600356023E-4	7.923365211672702E-5	1.254532825181511E-4	6.602804343060584E-5	0.0
25-29	8.913785863131789E-4	1.1554907600356023E-4	1.1224767383202993E-4	1.6837151074804488E-4	6.932944560213614E-5	0.0
30-34	9.408996188861333E-4	1.35357489032742E-4	1.2875468468968138E-4	1.8157711943416608E-4	9.243926080284819E-5	0.0
35-39	0.001049845890546633	1.650701085765146E-4	1.4526169554733283E-4	2.2779674983559013E-4	9.904206514590877E-5	0.0
40-44	0.0012479300208384507	1.650701085765146E-4	1.650701085765146E-4	2.641121737224234E-4	1.0894627166049965E-4	0.0
45-49	0.0014889323793601619	2.0138553246334782E-4	1.8817992377722666E-4	3.433458258391504E-4	1.7827571726263577E-4	0.0
50-54	0.0017299347378818733	2.178925433209993E-4	2.1459114114946902E-4	3.928668584121048E-4	1.9808413029181755E-4	6.602804343060585E-6
55-59	0.001931320270345221	2.3109815200712046E-4	2.542079672078325E-4	4.2588088012740776E-4	2.14591141149469E-4	1.9808413029181756E-5
60-64	0.0021029931832647966	2.4100235852171132E-4	2.641121737224234E-4	4.489906953281198E-4	2.905233910946657E-4	3.6315423886833214E-5
65-69	0.0022977759113850835	2.542079672078325E-4	2.773177824085446E-4	5.08415934415665E-4	3.8956545624057443E-4	7.593224994519672E-5
70-74	0.0050940635506712415	2.641121737224234E-4	2.971261954377263E-4	5.678411735032103E-4	4.29182282298938E-4	8.58364564597876E-5
75-79	0.024456787286696403	2.641121737224234E-4	3.2353741280996865E-4	6.074579995615738E-4	4.522920974996501E-4	9.904206514590877E-5
80-84	0.02893348863129148	2.70714978065484E-4	3.49948630182211E-4	6.470748256199373E-4	6.635818364775888E-4	9.904206514590877E-5
85-89	0.028969804055178315	2.9382479326619604E-4	3.796612497259836E-4	7.098014668790128E-4	8.088435320249216E-4	9.904206514590877E-5
90-94	0.0289896124682075	3.1363320629537776E-4	3.8296265189751387E-4	7.461168907658461E-4	8.088435320249216E-4	9.904206514590877E-5
95-99	0.02901932508775127	3.301402171530292E-4	4.126752714412866E-4	7.79130912481149E-4	8.121449341964519E-4	9.904206514590877E-5
100-104	0.02904573630512351	3.301402171530292E-4	4.1597667361281687E-4	8.319533472256337E-4	8.253505428825732E-4	9.904206514590877E-5
105-109	0.029101860142039526	3.301402171530292E-4	4.3908648881352887E-4	8.649673689409366E-4	8.253505428825732E-4	9.904206514590877E-5
110-114	0.029194299402842377	3.301402171530292E-4	4.6879910835730153E-4	8.913785863131789E-4	8.286519450541035E-4	9.904206514590877E-5
115-119	0.029257026044101454	3.4664722801068065E-4	5.018131300726044E-4	9.012827928277699E-4	8.484603580832851E-4	9.904206514590877E-5
120-124	0.02934616390273277	3.796612497259836E-4	5.447313583024983E-4	9.541052275722545E-4	8.715701732839972E-4	1.0234346731743908E-4
125-129	0.02949802840262316	3.796612497259836E-4	5.843481843608618E-4	9.739136406014361E-4	9.078855971708305E-4	1.1554907600356023E-4
130-134	0.029791853195889357	3.9946966275516537E-4	6.20663608247695E-4	0.0010267360753459208	9.243926080284818E-4	1.2875468468968138E-4
135-139	0.03002295134789648	4.225794779558775E-4	6.635818364775888E-4	0.0010564486948896935	9.871192492875572E-4	1.320560868612117E-4
140-144	0.03023754248904595	4.5889490184271065E-4	6.767874451637099E-4	0.0010861613144334662	0.0010366402818605118	1.3865889120427227E-4
145-149	0.030432325217166233	5.051145322441347E-4	6.99897260364422E-4	0.001115873933977239	0.001096065520948057	1.4856309771886316E-4
150-154	0.03054787429316979	5.282243474448468E-4	7.428154885943158E-4	0.00112907954266336	0.001138983749177951	1.4856309771886316E-4
155-159	0.03067662897785947	5.678411735032103E-4	7.659253037950277E-4	0.0011554907600356023	0.001138983749177951	1.4856309771886316E-4
160-164	0.030914329934209655	5.843481843608617E-4	7.923365211672702E-4	0.0011719977708932539	0.0011554907600356023	1.4856309771886316E-4
165-166	0.031140475982959485	5.942523908754526E-4	8.088435320249216E-4	0.0011719977708932539	0.0011885047817509053	1.650701085765146E-4
>>END_MODULE
