##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293549
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6440038
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.38363733257474	39.0	39.0	39.0	37.0	39.0
2	40.16647743382881	41.0	40.0	41.0	38.0	41.0
3	40.20895730739477	41.0	40.0	41.0	38.0	41.0
4	40.220717952285376	41.0	40.0	41.0	38.0	41.0
5	40.217194681149394	41.0	40.0	41.0	38.0	41.0
6	40.20823588308019	41.0	40.0	41.0	38.0	41.0
7	40.230191654148626	41.0	40.0	41.0	38.0	41.0
8	40.2120791833837	41.0	40.0	41.0	38.0	41.0
9	40.191592968861364	41.0	40.0	41.0	38.0	41.0
10-14	40.1286137131489	41.0	40.0	41.0	38.0	41.0
15-19	40.00551046437924	41.0	40.0	41.0	38.0	41.0
20-24	39.812899520158105	41.0	40.0	41.0	37.6	41.0
25-29	39.54604388980313	40.6	39.0	41.0	36.4	41.0
30-34	39.347826798537525	40.0	39.0	41.0	35.4	41.0
35-39	39.128237814745816	40.0	38.0	41.0	35.0	41.0
40-44	38.56531026680277	39.8	36.4	41.0	35.0	41.0
45-49	37.890636545933425	38.8	35.0	41.0	35.0	41.0
50-54	37.20227775053501	36.8	35.0	40.2	34.0	41.0
55-59	36.540805100839464	35.6	35.0	39.0	34.0	41.0
60-64	35.832619000074224	35.0	34.8	37.0	33.6	40.0
65-69	35.49515521492265	35.0	35.0	36.0	33.6	39.0
70-74	35.291359398811	35.0	35.0	36.0	33.8	37.8
75-79	35.110586303993855	35.0	35.0	35.0	33.0	37.0
80-84	35.03025066001163	35.0	35.0	35.0	33.0	37.0
85-89	34.83636351214077	35.0	34.6	35.0	32.6	37.2
90-94	39.59363720911939	40.6	39.8	40.6	37.8	40.6
95-99	39.856711894174154	41.0	40.0	41.0	37.6	41.0
100-104	39.730608097931935	41.0	40.0	41.0	37.0	41.0
105-109	39.57094095796195	41.0	39.2	41.0	36.0	41.0
110-114	39.36859834242394	40.4	39.0	41.0	35.2	41.0
115-119	39.06656715019598	40.0	38.0	41.0	35.0	41.0
120-124	38.76204673582424	40.0	37.4	41.0	35.0	41.0
125-129	38.20578154527799	39.6	36.0	40.6	34.4	41.0
130-134	38.03678843483325	39.0	35.0	41.0	34.4	41.0
135-139	37.63319980878524	38.0	35.0	41.0	34.0	41.0
140-144	37.001127285980765	36.6	35.0	39.6	34.0	41.0
145-149	36.35825440250299	35.4	35.0	38.4	34.0	41.0
150-154	35.759253923651954	35.0	35.0	36.8	34.0	39.4
155-159	35.2765733501288	35.0	35.0	36.0	33.2	37.8
160-164	34.92952842996658	35.0	35.0	35.0	33.0	36.6
165-169	34.7037466079817	35.0	35.0	35.0	33.0	36.0
170-174	34.573373436113016	35.0	35.0	35.0	33.0	36.0
175-178	34.26422354569513	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	28.0
30	340.0
31	2739.0
32	14107.0
33	54519.0
34	156311.0
35	395443.0
36	1071251.0
37	2055826.0
38	2040418.0
39	638209.0
40	10847.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.008249330205814	26.492700819467213	26.901099030782117	23.597950819544852
2	23.820309755936222	26.807962934380203	27.02667282398023	22.345054485703344
3	22.0324010510497	26.145560010670742	28.042614034265018	23.779424904014544
4	22.794741273265778	25.445470973929034	28.1790728564024	23.580714896402785
5	23.477237246115628	26.12009432242481	26.80540083769692	23.597267593762645
6	23.480047788537895	26.184736798136903	26.737947819562557	23.597267593762645
7	23.56495411983594	26.326040312184496	26.403011907693713	23.705993660285856
8	23.568541055192533	26.59029341131217	26.350201660300765	23.490963873194538
9	23.628136976831506	26.5746723854735	26.37167668886426	23.425513948830737
10-14	23.61476438493065	26.54212288809476	26.279335618827094	23.563777108147498
15-19	23.61745070448342	26.464685456824945	26.348397323121386	23.56946651557025
20-24	23.566941685747818	26.412682658083696	26.40338457630219	23.6169910798663
25-29	23.604158857447736	26.40451500441457	26.2762145192311	23.715111618906594
30-34	23.674965893058395	26.40776964359527	26.24053460554115	23.67672985780519
35-39	23.639804609848575	26.417368965835298	26.27911822880548	23.663708195510647
40-44	23.666981468121772	26.407928027753876	26.225084386148033	23.700006117976322
45-49	23.781393836495994	26.320083825592334	26.1852523230453	23.71327001486637
50-54	23.757167271373245	26.384157981676502	26.196982067497114	23.661692679453132
55-59	23.725953169841542	26.346155100327046	26.18563430836899	23.74225742146242
60-64	23.716636454629615	26.365605296117817	26.201000677325197	23.71675757192737
65-69	23.70117381294955	26.374139407251945	26.221904280689028	23.702782499109475
70-74	23.647897729796004	26.36523262750934	26.23298185507601	23.753887787618645
75-79	23.671748520738543	26.338943962753014	26.24406564060647	23.745241875901975
80-84	23.63876113774484	26.39017347413167	26.229988083921246	23.741077304202243
85-89	23.638565486725387	26.351310349410983	26.184587730693515	23.825536433170114
90-94	22.98181000227174	26.21356777691962	27.355996964306247	23.448625256502392
95-99	23.440839175900248	26.439925081840705	26.491741568660515	23.62749417359854
100-104	23.51784632774343	26.506893314380285	26.39187655401229	23.583383803863992
105-109	23.45144048052215	26.44956299411839	26.4573758396663	23.64162068569316
110-114	23.48646401601593	26.341402210306246	26.459694275538286	23.712439498139542
115-119	23.489996820895406	26.409021428233558	26.354315056965373	23.746666693905656
120-124	23.4745619045614	26.393161280625538	26.35448999591982	23.77778681889324
125-129	23.503483999493717	26.37335906911012	26.367393647812627	23.75576328358354
130-134	23.595266920352266	26.390654845605088	26.29297284891965	23.721105385122996
135-139	23.646883230722096	26.343570331435203	26.28173574474278	23.727810693099922
140-144	23.627196966014033	26.391098406693164	26.324859979879744	23.656844647413052
145-149	23.57293986239586	26.45513533275347	26.342195975072546	23.629728829778127
150-154	23.511160373817532	26.510403269070014	26.36711934468421	23.611317012428245
155-159	23.47685868329035	26.50764990588043	26.44636996275168	23.56912144807754
160-164	23.415260047630817	26.63035686292668	26.470962663532188	23.483420425910314
165-169	23.36163600988983	26.645605899511928	26.506259588717878	23.486498501880362
170-174	23.308231034946445	26.73101980034222	26.564563544726198	23.396185619985136
175-178	23.23380995688233	26.83692182475189	26.542317963527896	23.386950254837892
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	1.0
11	5.5
12	12.5
13	27.5
14	66.0
15	131.0
16	233.5
17	461.0
18	889.0
19	1601.0
20	2808.0
21	4564.0
22	7037.5
23	10606.5
24	15140.0
25	20704.0
26	27307.0
27	34838.0
28	43745.5
29	53770.5
30	64326.0
31	75262.5
32	86555.0
33	98522.5
34	110645.0
35	123163.0
36	136076.0
37	148977.0
38	162238.0
39	175616.0
40	189074.0
41	201687.5
42	213805.5
43	225288.5
44	233888.0
45	241042.5
46	246356.5
47	247998.0
48	246209.0
49	241500.5
50	234791.0
51	225573.5
52	214227.0
53	202140.5
54	190262.5
55	179277.5
56	168517.5
57	157630.5
58	147984.5
59	138849.5
60	129034.0
61	119475.5
62	109453.5
63	98271.0
64	86747.5
65	75013.5
66	63443.0
67	52192.5
68	41907.0
69	32843.5
70	24809.5
71	18321.5
72	13037.5
73	8913.5
74	6038.0
75	3903.5
76	2411.5
77	1456.5
78	834.0
79	457.5
80	271.5
81	139.0
82	63.5
83	30.5
84	16.0
85	7.5
86	3.5
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	667.0
92-93	1657.0
94-95	2533.0
96-97	3237.0
98-99	4108.0
100-101	4698.0
102-103	5708.0
104-105	6366.0
106-107	7138.0
108-109	7937.0
110-111	8619.0
112-113	9189.0
114-115	9703.0
116-117	9970.0
118-119	10603.0
120-121	11342.0
122-123	11703.0
124-125	12060.0
126-127	12736.0
128-129	13190.0
130-131	13862.0
132-133	14847.0
134-135	57255.0
136-137	64380.0
138-139	60804.0
140-141	59373.0
142-143	64412.0
144-145	63863.0
146-147	62221.0
148-149	63289.0
150-151	63700.0
152-153	60026.0
154-155	64864.0
156-157	62003.0
158-159	68550.0
160-161	65426.0
162-163	66159.0
164-165	67295.0
166-167	67243.0
168-169	67976.0
170-171	71148.0
172-173	72527.0
174-175	74734.0
176-177	197701.0
178-179	4693216.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.28236763462047
#Duplication Level	Percentage of total
1	94.81772738697669
2	4.551224084046143
3	0.44669911156318454
4	0.10538314899131446
5	0.040212512100122916
6	0.02182074929166412
7	0.008376936761337758
8	0.0021103828552226753
9	0.0031899188460648344
>10	0.0032557685682679843
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	3.105571737309624E-5	0.0	0.0	0.0	0.0
2	0.0	3.105571737309624E-5	0.0	0.0	0.0	0.0
3	0.0	4.658357605964437E-5	0.0	0.0	1.552785868654812E-5	0.0
4	0.0	4.658357605964437E-5	1.552785868654812E-5	0.0	1.552785868654812E-5	0.0
5	0.0	4.658357605964437E-5	1.552785868654812E-5	0.0	1.552785868654812E-5	0.0
6	0.0	4.658357605964437E-5	3.105571737309624E-5	1.552785868654812E-5	1.552785868654812E-5	0.0
7	0.0	6.211143474619248E-5	3.105571737309624E-5	1.552785868654812E-5	3.105571737309624E-5	0.0
8	1.552785868654812E-5	7.763929343274061E-5	3.105571737309624E-5	4.658357605964437E-5	4.658357605964437E-5	0.0
9	1.552785868654812E-5	9.316715211928873E-5	3.105571737309624E-5	4.658357605964437E-5	4.658357605964437E-5	0.0
10-14	3.105571737309624E-5	1.4285629991624274E-4	4.658357605964437E-5	7.763929343274061E-5	5.5900291271573236E-5	0.0
15-19	3.105571737309624E-5	1.7391201728933898E-4	6.211143474619248E-5	8.695600864466949E-5	6.211143474619248E-5	0.0
20-24	3.105571737309624E-5	2.0807330639974485E-4	8.385043690735986E-5	1.1180058254314647E-4	7.142814995812137E-5	0.0
25-29	4.037243258502512E-5	2.2360116508629295E-4	1.2111729775507536E-4	1.304340129670042E-4	8.074486517005024E-5	0.0
30-34	9.627272385659836E-5	2.3291788029822184E-4	1.3664515644162348E-4	1.4285629991624274E-4	9.937829559390798E-5	0.0
35-39	1.770175890266486E-4	2.763958846205565E-4	1.801231607639582E-4	1.925454477131967E-4	1.7080644555202934E-4	0.0
40-44	2.763958846205566E-4	3.1676831720558167E-4	2.0186216292512562E-4	2.142844498743641E-4	1.8943987597588708E-4	9.316715211928873E-6
45-49	3.944076106383223E-4	3.509296063159876E-4	2.2049559334898333E-4	2.422345955101507E-4	2.3602345203553146E-4	1.552785868654812E-5
50-54	5.621084844530421E-4	4.0372432585025124E-4	2.763958846205565E-4	2.5155131072207955E-4	2.763958846205565E-4	3.726686084771549E-5
55-59	6.925424974200463E-4	4.192521845367993E-4	2.919237433071047E-4	3.1055717373096244E-4	3.198738889428913E-4	8.074486517005024E-5
60-64	7.732873625900964E-4	4.503079019098955E-4	3.2608503241751056E-4	3.38507319366749E-4	3.4782403457867797E-4	1.2732844122969458E-4
65-69	8.229765103870506E-4	4.5651904538451484E-4	3.416128911040587E-4	3.6335189326522604E-4	3.8819646716370306E-4	1.397507281789331E-4
70-74	0.0015124134360697873	5.186304801307073E-4	3.6335189326522604E-4	3.8819646716370306E-4	4.4099118669796666E-4	1.397507281789331E-4
75-79	0.012925389570682658	5.403694822918746E-4	3.7887975195177416E-4	4.130410410621801E-4	4.658357605964437E-4	1.397507281789331E-4
80-84	0.018037160650294298	5.558973409784228E-4	4.161466127994897E-4	4.8757476275761104E-4	5.403694822918747E-4	1.397507281789331E-4
85-89	0.0181023776567778	5.590029127157324E-4	4.192521845367993E-4	5.279471953426361E-4	8.012375082258833E-4	1.397507281789331E-4
90-94	0.018161383519786686	5.776363431395902E-4	4.316744714860378E-4	5.900586300888286E-4	8.074486517005025E-4	1.397507281789331E-4
95-99	0.018254550671905977	6.180087757246153E-4	4.4099118669796666E-4	6.552756365723307E-4	8.074486517005025E-4	1.397507281789331E-4
100-104	0.01827628967406714	6.521700648350211E-4	4.5962461712182446E-4	6.894369256827367E-4	8.074486517005025E-4	1.397507281789331E-4
105-109	0.018332189965338714	6.739090669961885E-4	5.062081931814688E-4	7.422316452170003E-4	8.074486517005025E-4	1.397507281789331E-4
110-114	0.01835392896749988	6.925424974200463E-4	5.124193366560881E-4	7.732873625900965E-4	8.074486517005025E-4	1.397507281789331E-4
115-119	0.018406723687034145	7.049647843692848E-4	5.279471953426361E-4	7.763929343274061E-4	8.136597951751217E-4	1.397507281789331E-4
120-124	0.018512313126102676	7.329149300050714E-4	5.683196279276612E-4	8.012375082258833E-4	8.229765103870506E-4	1.397507281789331E-4
125-129	0.018763864436824755	7.60865075640858E-4	5.931642018261382E-4	8.35398797336289E-4	8.44715512548218E-4	1.397507281789331E-4
130-134	0.01901231017580952	7.857096495393351E-4	6.055864887753768E-4	8.757712299213141E-4	9.0993251903172E-4	1.397507281789331E-4
135-139	0.01921727791047196	8.043430799631928E-4	6.211143474619249E-4	8.85087945133243E-4	9.223548059809586E-4	1.397507281789331E-4
140-144	0.01940050664297322	8.136597951751217E-4	6.211143474619249E-4	8.912990886078623E-4	9.658328103032932E-4	1.397507281789331E-4
145-149	0.019568207516787943	8.260820821243602E-4	6.211143474619249E-4	9.130380907690297E-4	9.782550972525317E-4	1.397507281789331E-4
150-154	0.01966448024064454	8.540322277601467E-4	6.211143474619249E-4	9.409882364048163E-4	0.0010155219581002473	1.397507281789331E-4
155-159	0.01979801982534886	8.788768016586238E-4	6.211143474619249E-4	9.689383820406027E-4	0.0010403665319987242	1.397507281789331E-4
160-164	0.019947087268739717	8.85087945133243E-4	6.490644930977115E-4	9.782550972525317E-4	0.0010403665319987242	1.4906744339086196E-4
165-167	0.020036113658542593	8.954398509242751E-4	6.676979235215693E-4	9.782550972525317E-4	0.0010403665319987242	1.656304926565133E-4
>>END_MODULE
