##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293554
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5259145
Total Bases	850 Mbp
Sequences flagged as poor quality	0
Sequence length	90-174
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.20539878630462	40.0	39.0	41.0	36.0	41.0
2	39.36025665768865	40.0	39.0	41.0	36.0	41.0
3	39.45997096486216	40.0	39.0	41.0	37.0	41.0
4	39.45536451267269	40.0	39.0	41.0	37.0	41.0
5	39.46105327006576	40.0	39.0	41.0	37.0	41.0
6	39.44868224777982	40.0	39.0	41.0	37.0	41.0
7	39.42737098901057	40.0	39.0	41.0	37.0	41.0
8	39.41868003259085	40.0	39.0	41.0	37.0	41.0
9	39.37676713610292	40.0	39.0	41.0	36.0	41.0
10-14	39.28962616546986	40.0	39.0	41.0	36.0	41.0
15-19	39.07864525507473	40.0	38.0	41.0	35.6	41.0
20-24	38.80104051133787	40.0	38.0	41.0	35.0	41.0
25-29	38.55729777368755	40.0	37.6	41.0	34.8	41.0
30-34	38.09762400542293	39.4	36.4	40.2	33.8	41.0
35-39	37.67764262061608	38.8	35.0	40.0	33.4	41.0
40-44	37.091002320719426	37.4	35.0	40.0	33.0	41.0
45-49	36.38906464834113	35.8	35.0	39.4	33.0	41.0
50-54	35.8062645924385	35.0	34.0	38.2	33.0	40.0
55-59	35.30637839420667	35.0	34.0	36.8	32.0	39.4
60-64	34.79596459120256	35.0	33.6	36.0	31.8	39.0
65-69	34.76176895674107	35.0	34.0	35.6	32.0	37.6
70-74	34.62864336313222	35.0	34.0	35.0	32.0	37.0
75-79	34.589677561656885	35.0	34.0	35.0	32.0	37.6
80-84	34.588636898203035	35.0	34.0	35.0	32.0	38.0
85-89	37.29935356412497	38.6	36.8	39.4	32.2	40.2
90-94	38.482479029931056	40.0	38.0	41.0	33.0	41.0
95-99	38.33528362872229	40.0	37.0	41.0	33.0	41.0
100-104	38.1758883923585	40.0	37.0	41.0	33.0	41.0
105-109	37.94557031841451	40.0	36.0	41.0	32.6	41.0
110-114	37.65692691328421	39.2	35.2	40.2	32.0	41.0
115-119	37.29043844585019	38.6	35.0	40.0	32.0	41.0
120-124	36.87282997400186	37.8	34.8	39.8	31.8	40.8
125-129	36.46576381691053	37.0	34.0	40.0	31.0	40.8
130-134	36.11947753767616	36.0	34.0	40.0	31.0	41.0
135-139	35.89583180397382	35.2	34.0	39.0	31.0	41.0
140-144	35.40897919084186	35.0	34.0	37.6	31.0	40.0
145-149	34.92372499876209	35.0	34.0	36.4	31.0	39.0
150-154	34.51436401832287	35.0	34.0	35.6	31.0	37.4
155-159	34.196960138222416	35.0	33.2	35.0	31.0	36.4
160-164	33.98983121311038	35.0	33.0	35.0	31.0	36.0
165-169	33.88856073716526	35.0	33.0	35.0	31.0	35.4
170-174	33.667663893687156	34.8	32.8	35.0	30.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	5.0
28	111.0
29	1393.0
30	8519.0
31	36383.0
32	111789.0
33	236430.0
34	429033.0
35	788100.0
36	1269666.0
37	1338227.0
38	869723.0
39	168579.0
40	1187.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.462121694686115	25.854392681700162	27.261769736335467	23.421715887278253
2	23.439950790480204	25.90219512867586	27.011329788397166	23.646524292446777
3	23.275380313720195	26.502444789029393	26.78882213743869	23.43335275981172
4	23.560806937249307	26.35601414298332	26.466127098606336	23.617051821161045
5	23.907441228564718	26.37808997470121	26.084544160695323	23.629924636038748
6	23.41253188493567	26.753626302374244	26.412411142875886	23.4214306698142
7	23.542990352994643	26.577514025568792	26.357839534753275	23.521656086683294
8	23.99384310567592	26.39197055795191	26.042331976015117	23.571854360357055
9	23.47052610262695	26.576297097722158	26.250635797263623	23.70254100238727
10-14	23.576539532566606	26.473348804796217	26.3427534323545	23.607358230282678
15-19	23.38739091620406	26.452311164647487	26.529335091540545	23.63096282760791
20-24	23.490209910546294	26.33540623048043	26.389875160315984	23.78450869865729
25-29	23.504980372284848	26.458635386550476	26.308443672878383	23.72794056828629
30-34	23.354659360029054	26.442872368037012	26.438772842353654	23.763695429580284
35-39	23.509589486503984	26.488921678333643	26.333983946059675	23.6675048891027
40-44	23.586088613263183	26.36049015571923	26.267258271068776	23.78616295994881
45-49	23.72742717685099	26.403200520236652	26.177829285939065	23.69154301697329
50-54	23.726008695329753	26.33880982555149	26.218288333940215	23.716893145178542
55-59	23.682161263855626	26.404105610322592	26.17205268156706	23.741680444254722
60-64	23.537042618144206	26.410194052455292	26.329591597113218	23.72317173228728
65-69	23.58689482796158	26.45814861541182	26.233522749420295	23.721433807206303
70-74	23.51376887307728	26.48411481333943	26.26340973675379	23.738706576829504
75-79	23.42938633561159	26.52829689997138	26.285649093151076	23.75666767126596
80-84	23.458204708179753	26.536370455653913	26.246068514939214	23.75935632122712
85-89	22.4362325054738	26.327214784912755	27.862179118468877	23.37437359114457
90-94	23.108100847713402	26.461318976120136	26.75360560148049	23.676974574685975
95-99	23.275780846003972	26.692150854987368	26.380626419981496	23.651441879027164
100-104	23.238397487130396	26.6528719879696	26.500823399932088	23.607907124967912
105-109	23.22215720619005	26.51680739557473	26.534212975135134	23.72682242310008
110-114	23.245094654362628	26.50960980571006	26.413220593163544	23.832074946763765
115-119	23.228827936321437	26.59237461370344	26.42691555547855	23.75188189449657
120-124	23.192069852417816	26.587452658519428	26.45913487141017	23.761342617652588
125-129	23.281239787655863	26.645784061924832	26.37016676228447	23.702809388134842
130-134	23.554440315243557	26.52363014563272	26.242176008209224	23.679753530914496
135-139	23.449646824268573	26.68424956500401	26.344347033303002	23.521756577424423
140-144	23.345358162568246	26.684809980531952	26.448542460665575	23.521289396234224
145-149	23.317198256051356	26.849242347319237	26.46131066853477	23.37224872809464
150-154	23.177088499765304	26.88784364335845	26.59162749126415	23.343440365612093
155-159	23.04834235886589	27.090096675858845	26.658668819160436	23.202892146114827
160-164	22.9805953869361	27.18240933009646	26.76121005282778	23.075785230139665
165-169	22.845600240518422	27.364146010460637	26.846087009830867	22.944166739190067
170-174	22.68723207414272	27.539302091370736	26.953862564830487	22.819603269656053
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	7.5
13	12.5
14	24.5
15	53.0
16	104.5
17	225.5
18	420.5
19	821.0
20	1545.5
21	2782.5
22	4732.5
23	7604.5
24	11622.5
25	16912.5
26	23608.5
27	31558.0
28	40633.0
29	50916.0
30	62125.0
31	73701.5
32	85903.0
33	98186.0
34	109641.0
35	120878.0
36	131993.0
37	142680.0
38	152164.5
39	160762.0
40	168076.5
41	173777.5
42	177856.5
43	179780.0
44	180070.0
45	178268.5
46	174713.5
47	169622.0
48	162920.0
49	155970.0
50	149414.5
51	143308.0
52	138267.5
53	133700.5
54	130054.5
55	128014.5
56	126822.5
57	125849.0
58	124466.0
59	122588.0
60	119347.0
61	113907.5
62	107075.0
63	98832.5
64	88848.0
65	77867.5
66	66270.5
67	54591.5
68	43722.0
69	33859.0
70	25396.0
71	18465.5
72	12946.0
73	8892.5
74	5889.0
75	3772.0
76	2373.0
77	1432.0
78	863.0
79	485.0
80	258.5
81	146.5
82	79.0
83	40.0
84	17.5
85	7.5
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2454.0
92-93	4520.0
94-95	6695.0
96-97	8536.0
98-99	10344.0
100-101	12212.0
102-103	13774.0
104-105	15554.0
106-107	16812.0
108-109	17863.0
110-111	18778.0
112-113	20009.0
114-115	21168.0
116-117	22049.0
118-119	22863.0
120-121	24048.0
122-123	24857.0
124-125	25822.0
126-127	26904.0
128-129	28579.0
130-131	93445.0
132-133	81261.0
134-135	91044.0
136-137	93051.0
138-139	92574.0
140-141	97443.0
142-143	93382.0
144-145	91725.0
146-147	92572.0
148-149	90947.0
150-151	87189.0
152-153	84276.0
154-155	81200.0
156-157	84387.0
158-159	85478.0
160-161	89495.0
162-163	88949.0
164-165	86868.0
166-167	89279.0
168-169	87338.0
170-171	97439.0
172-173	194269.0
174-175	2841693.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.2757895861763
#Duplication Level	Percentage of total
1	91.0125234391274
2	7.845780198902269
3	0.8156322864698213
4	0.17270593962761238
5	0.07576062098636978
6	0.03143049133786033
7	0.012691325720850345
8	0.007473192949722391
9	0.004310594235496297
>10	0.021691910642567626
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.901449760369794E-5	3.802899520739588E-5	0.0	0.0	0.0
2	3.802899520739588E-5	1.901449760369794E-5	7.605799041479176E-5	0.0	0.0	0.0
3	3.802899520739588E-5	1.901449760369794E-5	7.605799041479176E-5	0.0	0.0	0.0
4	3.802899520739588E-5	1.901449760369794E-5	7.605799041479176E-5	0.0	0.0	0.0
5	5.7043492811093815E-5	1.901449760369794E-5	7.605799041479176E-5	0.0	0.0	0.0
6	5.7043492811093815E-5	3.802899520739588E-5	7.605799041479176E-5	0.0	0.0	0.0
7	5.7043492811093815E-5	3.802899520739588E-5	9.50724880184897E-5	0.0	0.0	0.0
8	7.605799041479176E-5	3.802899520739588E-5	9.50724880184897E-5	0.0	0.0	0.0
9	7.605799041479176E-5	3.802899520739588E-5	9.50724880184897E-5	0.0	0.0	0.0
10-14	9.126958849775011E-5	6.845219137331258E-5	1.4451018178810433E-4	0.0	0.0	0.0
15-19	1.3310148322588557E-4	9.50724880184897E-5	2.0155367459919818E-4	3.802899520739588E-6	1.901449760369794E-5	0.0
20-24	1.93947875557719E-4	9.50724880184897E-5	2.0915947364067737E-4	5.324059329035423E-5	1.901449760369794E-5	0.0
25-29	2.2056817220289607E-4	1.1408698562218763E-4	2.2817397124437526E-4	1.1408698562218763E-4	4.5634794248875054E-5	0.0
30-34	3.955015501569171E-4	1.5972177987106268E-4	2.3958266980659404E-4	1.6732757891254187E-4	9.887538753922927E-5	0.0
35-39	0.0011294611576596575	2.2817397124437526E-4	2.852174640554691E-4	2.0155367459919813E-4	1.4451018178810436E-4	0.0
40-44	0.0020649744397615963	2.4718846884807323E-4	2.966261626176879E-4	2.4338556932733364E-4	2.3958266980659404E-4	1.5211598082958352E-5
45-49	0.0029852761237805767	3.0042906213842744E-4	3.3465515782508373E-4	2.4718846884807323E-4	2.852174640554691E-4	1.901449760369794E-5
50-54	0.0037534618269699726	3.4226095686656287E-4	3.726841530324796E-4	2.4718846884807323E-4	3.422609568665629E-4	2.2817397124437527E-5
55-59	0.004342911252684609	4.33530545364313E-4	3.87895751115438E-4	2.6620296645177115E-4	4.33530545364313E-4	3.802899520739588E-5
60-64	0.004765033099486704	4.905740381754068E-4	4.145160477606151E-4	3.3465515782508373E-4	4.4493924392653174E-4	4.5634794248875054E-5
65-69	0.005202366544371756	5.019827367376255E-4	4.4493924392653174E-4	4.145160477606151E-4	5.324059329035423E-4	5.7043492811093815E-5
70-74	0.05395173550073253	5.209972343413235E-4	4.753624400924485E-4	4.1831894728135475E-4	5.970552247561153E-4	5.7043492811093815E-5
75-79	0.09519037790363263	5.324059329035423E-4	5.209972343413235E-4	4.1831894728135475E-4	6.997335118160842E-4	5.7043492811093815E-5
80-84	0.09560489395139324	5.818436266731569E-4	5.324059329035423E-4	4.4113634440579215E-4	9.773451768300741E-4	5.7043492811093815E-5
85-89	0.09590532301353166	6.122668228390737E-4	5.514204305072403E-4	4.867711386546673E-4	0.0010267828705996886	5.7043492811093815E-5
90-94	0.09613349698477605	6.27478420922032E-4	5.970552247561153E-4	4.943769376961465E-4	0.0010267828705996886	5.7043492811093815E-5
95-99	0.09636547385554114	6.426900190049904E-4	6.426900190049904E-4	5.133914352998443E-4	0.0010267828705996886	5.7043492811093815E-5
100-104	0.09661646522390997	6.997335118160842E-4	6.845219137331258E-4	5.133914352998443E-4	0.001041994468682647	5.7043492811093815E-5
105-109	0.09697013487933875	7.225509089405217E-4	7.111422103783029E-4	5.209972343413235E-4	0.001118052459097439	5.7043492811093815E-5
110-114	0.09738845382662012	7.377625070234802E-4	7.757915022308758E-4	5.324059329035423E-4	0.0011218553586181785	5.7043492811093815E-5
115-119	0.09773071478348669	7.605799041479176E-4	8.02411798876053E-4	5.324059329035423E-4	0.0011218553586181785	5.7043492811093815E-5
120-124	0.09847988598907237	7.681857031893967E-4	8.898784878530635E-4	5.324059329035423E-4	0.001182701750950012	5.7043492811093815E-5
125-129	0.10013414728059408	7.833973012723551E-4	9.355132821019386E-4	5.400117319450216E-4	0.0012701684399270224	5.7043492811093815E-5
130-134	0.1018074230697195	8.176233969590114E-4	9.697393777885951E-4	5.514204305072403E-4	0.0012777742389685014	7.225509089405216E-5
135-139	0.10325252488760055	8.176233969590114E-4	0.001007768372995991	5.514204305072403E-4	0.0012967887365721994	1.0267828705996886E-4
140-144	0.10469382380596086	8.632581912078864E-4	0.001007768372995991	5.666320285901986E-4	0.0013234090332173765	1.1408698562218763E-4
145-149	0.10627963290610927	8.784697892908448E-4	0.0010343886696411678	5.818436266731569E-4	0.0013652409279455119	1.2549568418440639E-4
150-154	0.10764487383405477	8.936813873738032E-4	0.0010457973682033866	6.008581242768549E-4	0.0014070728226736478	1.4451018178810436E-4
155-159	0.10885419588164998	8.974842868945428E-4	0.001061008966286345	6.084639233183341E-4	0.0015287656073373142	1.5211598082958352E-4
160-163	0.10991805702257687	9.554785045858215E-4	0.0010838263634107826	6.084639233183341E-4	0.001559188803503231	1.5211598082958352E-4
>>END_MODULE
