##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293555
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5494861
Total Bases	889 Mbp
Sequences flagged as poor quality	0
Sequence length	90-174
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.247118716924774	40.0	39.0	41.0	36.0	41.0
2	39.40442970258938	40.0	39.0	41.0	36.0	41.0
3	39.488860955718444	40.0	39.0	41.0	37.0	41.0
4	39.50305512732715	40.0	39.0	41.0	37.0	41.0
5	39.50898557761516	40.0	39.0	41.0	37.0	41.0
6	39.47688631250181	40.0	39.0	41.0	37.0	41.0
7	39.46209103378593	40.0	39.0	41.0	37.0	41.0
8	39.46430728638995	40.0	39.0	41.0	37.0	41.0
9	39.425789842545605	40.0	39.0	41.0	37.0	41.0
10-14	39.32021221282941	40.0	39.0	41.0	36.0	41.0
15-19	39.10979022035316	40.0	38.0	41.0	35.8	41.0
20-24	38.82740811823993	40.0	38.0	41.0	35.0	41.0
25-29	38.57857019495125	40.0	37.6	41.0	34.8	41.0
30-34	38.10001028233471	39.4	36.4	40.4	33.8	41.0
35-39	37.672390038619724	38.8	35.0	40.0	33.4	41.0
40-44	37.080162391732934	37.4	35.0	40.0	33.0	41.0
45-49	36.378062083827054	35.8	35.0	39.4	33.0	41.0
50-54	35.80118452495887	35.0	34.0	38.2	33.0	40.0
55-59	35.31347038623907	35.0	34.0	36.8	32.4	39.4
60-64	34.80464586820303	35.0	33.8	36.0	31.8	39.0
65-69	34.77239693597345	35.0	34.0	35.6	32.0	37.6
70-74	34.64410120656373	35.0	34.0	35.0	32.0	37.0
75-79	34.606304727271535	35.0	34.0	35.0	32.0	37.8
80-84	34.614600769701	35.0	34.0	35.0	32.0	38.0
85-89	37.33430268754752	38.6	36.8	39.4	32.2	40.2
90-94	38.515101546045834	40.0	38.0	41.0	33.0	41.0
95-99	38.3753625790121	40.0	37.2	41.0	33.0	41.0
100-104	38.19961653898794	40.0	37.0	41.0	33.0	41.0
105-109	37.972288476383866	40.0	36.0	41.0	32.8	41.0
110-114	37.67852266456758	39.4	35.2	40.4	32.0	41.0
115-119	37.3095532253807	38.6	35.0	40.0	32.0	41.0
120-124	36.893463299650556	37.8	34.8	39.8	31.8	40.8
125-129	36.46518555743993	37.0	34.0	40.0	31.0	40.8
130-134	36.12660542020963	36.0	34.0	39.8	31.0	41.0
135-139	35.88099267145219	35.2	34.0	39.0	31.0	41.0
140-144	35.39917806016793	35.0	34.0	37.6	31.0	40.0
145-149	34.90705107196819	35.0	34.0	36.4	31.0	39.0
150-154	34.50020836808535	35.0	34.0	35.6	31.0	37.4
155-159	34.19140420801655	35.0	33.4	35.0	31.0	36.4
160-164	33.98737121834461	35.0	33.0	35.0	31.0	36.0
165-169	33.87734814423339	35.0	33.0	35.0	31.0	35.4
170-174	33.64624562814755	34.8	32.8	35.0	30.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	4.0
28	127.0
29	1528.0
30	9587.0
31	40500.0
32	120254.0
33	244941.0
34	436573.0
35	807062.0
36	1322289.0
37	1407521.0
38	921239.0
39	181931.0
40	1305.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.53679192248903	25.731624512430795	27.173098646171397	23.558484918908775
2	23.546200713721422	25.81544828886481	26.851998621985164	23.786352375428603
3	23.365650195701036	26.377282337078228	26.700548021141934	23.5565194460788
4	23.668424005629987	26.246141622144766	26.37429773018826	23.711136642036987
5	24.026249253620794	26.25076412305971	25.968263801395526	23.754722821923977
6	23.485216459524636	26.6781270718222	26.319300888593904	23.51735558005926
7	23.655630233412637	26.448239546004892	26.266870080972023	23.629260139610448
8	24.078698260065178	26.277789374471894	25.967954421413026	23.675557944049906
9	23.565200284411198	26.47928673718953	26.168814825343173	23.7866981530561
10-14	23.698521218280135	26.35041723530404	26.233307812517914	23.717753733897908
15-19	23.507586452141375	26.325768750110328	26.441567857676475	23.725076940071823
20-24	23.60066614969878	26.249937896518222	26.26182536737508	23.887570586407918
25-29	23.610391600442668	26.362115438406903	26.195727244055856	23.831765717094573
30-34	23.49769721199499	26.32761047094731	26.321801406805378	23.852890910252324
35-39	23.62394244367601	26.39201246400955	26.208746681672203	23.775298410642236
40-44	23.726725753390305	26.24512976761378	26.139722187695014	23.88842229130091
45-49	23.80648755264237	26.310660087671007	26.087142149728628	23.795710209957996
50-54	23.82515954452715	26.235091297122892	26.110443922057353	23.8293052362926
55-59	23.7867127121141	26.29158772169123	26.052091217594036	23.86960834860063
60-64	23.634308492971886	26.33269158218925	26.20148171173029	23.831518213108577
65-69	23.70420653042907	26.343811062736616	26.121537924253225	23.830444482581086
70-74	23.623480193584516	26.370723481449303	26.151642416432374	23.85415390853381
75-79	23.556293780679802	26.421796656912704	26.143041652918974	23.87886790948852
80-84	23.58460022919597	26.44031213892399	26.119812675880244	23.855274955999796
85-89	22.558703486766998	26.223029117570036	27.731718782331345	23.48654861333162
90-94	23.23448475122864	26.34347402927752	26.632416748160626	23.78962447133322
95-99	23.378658807238644	26.598797026025238	26.264147806758984	23.758396359977134
100-104	23.34765840964672	26.5675867344637	26.3741062489678	23.710648606921783
105-109	23.324337155766077	26.400202504570736	26.439504864937923	23.835955474725264
110-114	23.35069259291687	26.40758936914956	26.311319050656902	23.93039898727667
115-119	23.35109868500692	26.490851906012676	26.308256873546686	23.84979253543372
120-124	23.307499103634207	26.46297335008886	26.36717687517971	23.86235067109722
125-129	23.376292571126662	26.51974778106389	26.2713579423759	23.832601705433547
130-134	23.66042286423716	26.42542304303324	26.136522664326485	23.777631428403108
135-139	23.566746101599218	26.54322284404736	26.24337396416294	23.64665709019048
140-144	23.45917146281127	26.564138429963226	26.325238278903917	23.65145182832159
145-149	23.412086999037808	26.744783419965103	26.35561825904912	23.48751132194797
150-154	23.288782863421098	26.78354847826575	26.489683986820367	23.43798467149278
155-159	23.144868481544382	26.964777600994438	26.55160562617201	23.338748291289175
160-164	23.07269902350549	27.05078233560468	26.674502047523646	23.202016593366185
165-169	22.956453963820167	27.2208271506518	26.71610757941435	23.106611306113685
170-174	22.821640174522756	27.41036557609698	26.823815406762098	22.944178842618175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	3.5
12	6.0
13	16.0
14	28.5
15	47.5
16	99.0
17	214.0
18	448.5
19	862.5
20	1576.0
21	2809.5
22	4811.5
23	7691.0
24	11717.0
25	17085.0
26	23907.5
27	31958.0
28	41223.5
29	51644.0
30	62922.5
31	75015.5
32	87373.0
33	99466.5
34	111556.5
35	123870.5
36	135822.5
37	146879.5
38	156192.0
39	164548.5
40	172678.5
41	179082.0
42	183735.5
43	185826.5
44	185758.0
45	184433.5
46	180556.0
47	175496.0
48	169662.5
49	162983.5
50	156601.5
51	150538.0
52	145204.0
53	140893.0
54	138017.0
55	136161.0
56	134895.0
57	134099.0
58	132797.0
59	130814.5
60	127516.5
61	122298.5
62	115498.5
63	106839.5
64	95969.0
65	84246.0
66	72078.5
67	59319.0
68	47418.0
69	36896.0
70	27717.5
71	20074.5
72	14039.0
73	9635.5
74	6450.0
75	4079.5
76	2577.5
77	1591.0
78	922.0
79	551.5
80	288.0
81	147.5
82	76.5
83	39.0
84	21.5
85	8.5
86	3.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2202.0
92-93	4686.0
94-95	6879.0
96-97	8951.0
98-99	10871.0
100-101	12565.0
102-103	14140.0
104-105	16161.0
106-107	16903.0
108-109	18445.0
110-111	19251.0
112-113	20728.0
114-115	21579.0
116-117	22450.0
118-119	24050.0
120-121	24687.0
122-123	25480.0
124-125	26359.0
126-127	27740.0
128-129	29245.0
130-131	100631.0
132-133	82305.0
134-135	91257.0
136-137	99762.0
138-139	102116.0
140-141	94564.0
142-143	93083.0
144-145	93645.0
146-147	92386.0
148-149	93220.0
150-151	94896.0
152-153	84032.0
154-155	87421.0
156-157	86769.0
158-159	87459.0
160-161	86486.0
162-163	92141.0
164-165	88012.0
166-167	101395.0
168-169	85287.0
170-171	96475.0
172-173	193372.0
174-175	3014775.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.11813818713159
#Duplication Level	Percentage of total
1	90.72589840759501
2	8.034166463211635
3	0.9309540503335946
4	0.1745476243965691
5	0.05387830842667391
6	0.024047600687083236
7	0.023210667283353727
8	0.0074519810158055165
9	0.004296651935882407
>10	0.01998567352911113
>50	0.0015625715852793645
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.819882249978662E-5	5.459646749935986E-5	0.0	0.0	0.0
2	0.0	1.819882249978662E-5	5.459646749935986E-5	1.819882249978662E-5	0.0	0.0
3	0.0	1.819882249978662E-5	5.459646749935986E-5	1.819882249978662E-5	0.0	0.0
4	1.819882249978662E-5	1.819882249978662E-5	5.459646749935986E-5	1.819882249978662E-5	0.0	0.0
5	1.819882249978662E-5	1.819882249978662E-5	5.459646749935986E-5	5.459646749935986E-5	0.0	0.0
6	1.819882249978662E-5	3.639764499957324E-5	5.459646749935986E-5	7.279528999914648E-5	0.0	0.0
7	1.819882249978662E-5	7.279528999914648E-5	5.459646749935986E-5	7.279528999914648E-5	0.0	0.0
8	1.819882249978662E-5	7.279528999914648E-5	5.459646749935986E-5	7.279528999914648E-5	0.0	0.0
9	1.819882249978662E-5	7.279528999914648E-5	7.279528999914648E-5	9.099411249893309E-5	0.0	0.0
10-14	5.095670299940253E-5	9.099411249893309E-5	1.0191340599880506E-4	9.099411249893309E-5	7.2795289999146475E-6	0.0
15-19	5.459646749935986E-5	1.0919293499871972E-4	1.0919293499871972E-4	9.463387699889042E-5	1.819882249978662E-5	0.0
20-24	5.459646749935986E-5	1.1647246399863436E-4	1.2011222849859168E-4	1.4195081549833564E-4	6.187599649927451E-5	0.0
25-29	5.459646749935986E-5	1.2739175749850633E-4	1.2739175749850633E-4	1.7834846049790884E-4	8.371458349901845E-5	0.0
30-34	3.057402179964152E-4	1.6014963799812222E-4	1.2739175749850633E-4	1.8198822499786618E-4	1.1647246399863436E-4	3.6397644999573238E-6
35-39	0.0012666380459851487	1.8198822499786618E-4	1.7470869599795155E-4	1.9290751849773815E-4	1.6014963799812224E-4	1.819882249978662E-5
40-44	0.002358567395972346	2.1110634099752475E-4	2.0018704749765278E-4	2.1838586999743944E-4	1.8926775399778084E-4	1.819882249978662E-5
45-49	0.0032066325244624024	3.1301974699632986E-4	2.2202563449739678E-4	2.2202563449739678E-4	2.4022445699718335E-4	1.819882249978662E-5
50-54	0.003927305895453952	3.4213786299598837E-4	2.802618664967139E-4	2.3658469249722604E-4	3.057402179964152E-4	2.5478351499701266E-5
55-59	0.004531506802446869	3.4577762749594574E-4	3.0937998249637254E-4	2.6206304399692735E-4	3.82175272495519E-4	3.639764499957324E-5
60-64	0.005037434067940936	3.82175272495519E-4	3.166595114962872E-4	2.911811599965859E-4	4.258524464950069E-4	5.823623199931718E-5
65-69	0.005423249104936413	4.1493315299513493E-4	3.275788049961591E-4	2.911811599965859E-4	4.658898559945374E-4	7.279528999914648E-5
70-74	0.052489043853884566	4.258524464950069E-4	3.348583339960737E-4	2.911811599965859E-4	5.568839684934706E-4	7.279528999914648E-5
75-79	0.09175118351492423	4.4405126899479356E-4	3.4941739199590306E-4	2.911811599965859E-4	6.660769034921903E-4	7.279528999914648E-5
80-84	0.09212607925841983	4.986477364941533E-4	3.7125597899564705E-4	2.911811599965859E-4	9.135808894892882E-4	7.279528999914648E-5
85-89	0.09250461476641539	5.132067944939826E-4	4.0401385949526293E-4	2.9482092449654323E-4	9.463387699889041E-4	7.279528999914648E-5
90-94	0.09274119945891261	5.568839684934705E-4	4.367717399948789E-4	3.275788049961591E-4	9.463387699889041E-4	7.279528999914648E-5
95-99	0.09296322509341	6.114804359928303E-4	4.586103269946228E-4	3.275788049961591E-4	9.463387699889041E-4	7.279528999914648E-5
100-104	0.09319980978590724	6.478780809924037E-4	4.7316938499445207E-4	3.566969209958177E-4	9.681773569886481E-4	7.279528999914648E-5
105-109	0.09352374882640344	6.660769034921903E-4	4.768091494944094E-4	3.7489574349560436E-4	9.827364149884775E-4	7.279528999914648E-5
110-114	0.09385132763139958	6.733564324921049E-4	5.204863234938974E-4	4.294922109949642E-4	0.0010264135889879653	7.279528999914648E-5
115-119	0.09416070761389597	6.733564324921049E-4	5.641634974933852E-4	4.5497056249466545E-4	0.0010628112339875384	7.279528999914648E-5
120-124	0.09508520779688512	6.879154904919341E-4	6.042009069929157E-4	4.5497056249466545E-4	0.0010955691144871546	7.279528999914648E-5
125-129	0.09694512745636333	7.352324289913794E-4	6.333190229925743E-4	4.695296204944947E-4	0.0011574451109864288	7.279528999914648E-5
130-134	0.09885964358334087	7.716300739909526E-4	6.369587874925317E-4	4.913682074942388E-4	0.0012011222849859168	7.279528999914648E-5
135-139	0.10015175998082573	8.153072479904405E-4	6.733564324921048E-4	5.132067944939826E-4	0.001215681342985746	7.279528999914648E-5
140-144	0.10166226224830802	8.298663059902699E-4	6.915552549918915E-4	5.314056169937692E-4	0.001281197103984978	7.279528999914648E-5
145-149	0.10327103815728915	8.371458349901845E-4	7.170336064915927E-4	5.459646749935986E-4	0.0013321538069843806	7.279528999914648E-5
150-154	0.10493441053376965	8.371458349901845E-4	7.279528999914647E-4	5.641634974933852E-4	0.0013758309809838681	7.279528999914648E-5
155-159	0.10615373164125534	8.553446574899711E-4	7.279528999914647E-4	5.823623199931718E-4	0.0014923034449825026	7.279528999914648E-5
160-163	0.10725931010811739	8.917423024895443E-4	7.279528999914647E-4	5.823623199931718E-4	0.0015378005012319693	7.279528999914648E-5
>>END_MODULE
