##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293556
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4399031
Total Bases	703.1 Mbp
Sequences flagged as poor quality	0
Sequence length	90-173
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.15536580669698	40.0	39.0	41.0	36.0	41.0
2	39.353839061375105	40.0	39.0	41.0	36.0	41.0
3	39.39614246864821	40.0	39.0	41.0	37.0	41.0
4	39.42419637415603	40.0	39.0	41.0	37.0	41.0
5	39.392753085850046	40.0	39.0	41.0	37.0	41.0
6	39.39181378808197	40.0	39.0	41.0	37.0	41.0
7	39.37586322987949	40.0	39.0	41.0	36.0	41.0
8	39.352172103356395	40.0	39.0	41.0	36.0	41.0
9	39.31691888509083	40.0	39.0	41.0	36.0	41.0
10-14	39.20540669070074	40.0	38.6	41.0	36.0	41.0
15-19	38.99159874072267	40.0	38.0	41.0	35.4	41.0
20-24	38.702468111727335	40.0	38.0	40.8	35.0	41.0
25-29	38.4564790745962	40.0	37.2	40.6	34.4	41.0
30-34	37.90924905962245	39.2	35.6	40.0	33.6	41.0
35-39	37.52605185096446	38.6	35.0	40.0	33.0	41.0
40-44	36.85884523205224	36.8	35.0	40.0	33.0	41.0
45-49	36.14700391972686	35.6	34.2	39.0	33.0	40.4
50-54	35.558285267823756	35.0	34.0	37.4	32.2	40.0
55-59	34.96617627836676	35.0	33.8	36.4	31.8	39.0
60-64	34.71294605561998	35.0	33.8	36.0	32.0	38.2
65-69	34.5828359472802	35.0	33.8	35.0	32.0	37.0
70-74	34.49478778394605	35.0	33.0	35.0	32.0	37.0
75-79	34.46689136766711	35.0	33.0	35.0	32.0	37.4
80-84	34.33954295843789	34.8	32.8	35.0	31.2	38.2
85-89	37.7457024967544	39.8	36.2	40.6	31.6	40.6
90-94	38.037090832417256	40.0	36.4	41.0	32.0	41.0
95-99	37.89258882005089	40.0	36.0	41.0	32.0	41.0
100-104	37.711644836268604	40.0	35.4	41.0	32.0	41.0
105-109	37.469847031213455	39.2	35.0	40.0	31.2	41.0
110-114	37.179155512272374	38.8	35.0	40.0	31.0	41.0
115-119	36.83598645529339	38.0	34.0	40.0	31.0	41.0
120-124	36.34034461098795	37.2	33.6	39.6	30.4	40.6
125-129	36.056410290133215	36.4	33.4	40.0	30.0	41.0
130-134	35.727253291693145	35.2	33.0	39.2	30.2	41.0
135-139	35.4742047604858	35.0	33.0	38.8	30.0	40.6
140-144	35.01397922079407	35.0	33.0	37.0	30.0	39.8
145-149	34.57423974977339	35.0	33.0	36.0	30.0	38.8
150-154	34.20006817248969	35.0	33.0	35.2	30.0	37.0
155-159	33.92077558559962	35.0	33.0	35.0	30.0	36.0
160-164	33.741540916924414	35.0	33.0	35.0	30.0	36.0
165-169	33.65748368380454	35.0	33.0	35.0	30.0	35.0
170-173	33.41366540998584	34.75	32.75	35.0	29.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	3.0
28	164.0
29	1928.0
30	11659.0
31	48534.0
32	139299.0
33	261418.0
34	411962.0
35	691544.0
36	1039634.0
37	1046465.0
38	636368.0
39	109500.0
40	553.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.789557291139797	26.133505310601358	26.71374673195074	23.363190666308103
2	23.236071762167622	26.506428347515627	26.848731004623517	23.408768885693235
3	23.310860960061433	26.43854976243632	26.717702148495885	23.532887129006365
4	23.873780384816566	26.380377860487915	26.156987754803275	23.588853999892248
5	23.407882326812427	26.82197511224631	26.419045467058538	23.35109709388272
6	23.338503411319447	26.700902994318522	26.524500509316713	23.43609308504532
7	23.92299576884091	26.42566056024611	26.109340898029586	23.542002772883393
8	23.454847215216258	26.659780301616426	26.288334862836837	23.59703762033048
9	23.372192648790154	26.483241422940644	26.455121593823733	23.689444334445472
10-14	23.480407389718327	26.57735760443607	26.473121012332037	23.46911399351357
15-19	23.37664362901739	26.430443431746674	26.575543568572265	23.617369370663678
20-24	23.37202897638139	26.480918184027345	26.427465503198317	23.719587336392948
25-29	23.41859377667491	26.57516166628514	26.38754762128296	23.61869693575699
30-34	23.35886244038744	26.496107892851857	26.43423062942725	23.710799037333448
35-39	23.36427726924407	26.56339543867729	26.449847705096875	23.622479586981772
40-44	23.570272635041672	26.42791560232242	26.312344695911438	23.68946706672447
45-49	23.712212985086943	26.43520357096824	26.225716527116994	23.62686691682782
50-54	23.604439250371275	26.419404637066663	26.28736646775165	23.688789644810416
55-59	23.621165661255855	26.49980870787226	26.25581861096228	23.62320701990961
60-64	23.538947554586453	26.455949048779154	26.310280604978686	23.69482279165571
65-69	23.45494723724384	26.552079310193538	26.326220479010036	23.666752973552583
70-74	23.469227654908547	26.52335480245536	26.328507346276943	23.67891019635915
75-79	23.442949140390233	26.58685969705601	26.2589511190078	23.711240043545953
80-84	23.38616390746053	26.60223581056828	26.25567312437671	23.755927157594478
85-89	22.214624084258556	26.318659723016275	28.179364955600448	23.28735123712472
90-94	23.05162681014895	26.691114114275166	26.64915533622723	23.60810373934865
95-99	23.181067666380603	26.73941419646715	26.450563844682996	23.628954292469253
100-104	23.049802863588503	26.747294168336843	26.62471011240244	23.578192855672217
105-109	23.105238600063068	26.576518274318495	26.635169013327975	23.683074112290463
110-114	23.132957723927554	26.63502106259772	26.470712552808116	23.761308660666607
115-119	23.065615150972185	26.717660546272302	26.509753690204025	23.706970612551487
120-124	23.094122508121764	26.68614750465782	26.530329766387506	23.68940022083291
125-129	23.275675691376136	26.662998820254064	26.374437902133852	23.68688758623594
130-134	23.412407963750518	26.64823146384864	26.345360722241896	23.59399985015895
135-139	23.366085503062177	26.76974954249057	26.42086639403289	23.44329856041437
140-144	23.275138793998764	26.786329805497033	26.474626258231872	23.46390514227234
145-149	23.135654489628852	26.953758246183263	26.58642296570035	23.324164298487535
150-154	23.070297603691987	27.010258377590418	26.68744425225918	23.231999766458415
155-159	22.970342483438365	27.17391017141279	26.731683964360926	23.124063380787916
160-164	22.781798426836055	27.295233098583694	26.898900251127404	23.02406822345284
165-169	22.72923034203175	27.506396542464135	26.924903288633693	22.839469826870417
170-173	22.569120394120915	27.62151847694192	27.04009463478884	22.769266494148322
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.5
11	1.0
12	4.0
13	8.0
14	16.0
15	49.5
16	95.0
17	177.5
18	399.0
19	743.0
20	1320.0
21	2353.0
22	4041.0
23	6509.5
24	9904.5
25	14398.5
26	20102.0
27	26882.5
28	34667.5
29	43396.0
30	53062.0
31	63175.5
32	73038.0
33	83099.0
34	93147.5
35	103010.5
36	112507.0
37	120639.0
38	128450.5
39	135669.0
40	141743.5
41	146780.0
42	149834.5
43	151198.0
44	151098.0
45	149019.0
46	145859.5
47	141839.5
48	136222.0
49	130204.5
50	124414.0
51	119209.0
52	114953.0
53	111305.5
54	108553.0
55	106456.5
56	104957.0
57	104053.5
58	102803.5
59	101291.5
60	98493.0
61	93957.5
62	88280.5
63	81530.0
64	73544.5
65	64346.5
66	54454.0
67	44785.0
68	35700.0
69	27532.0
70	20626.0
71	14860.0
72	10434.0
73	7080.5
74	4640.0
75	2955.5
76	1833.0
77	1084.5
78	642.5
79	371.5
80	212.0
81	129.5
82	71.0
83	30.5
84	13.0
85	6.5
86	2.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-173	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1792.0
92-93	3970.0
94-95	5603.0
96-97	7612.0
98-99	9098.0
100-101	11045.0
102-103	12305.0
104-105	13438.0
106-107	14776.0
108-109	15427.0
110-111	16793.0
112-113	17899.0
114-115	19056.0
116-117	19335.0
118-119	20328.0
120-121	21428.0
122-123	22351.0
124-125	23158.0
126-127	23806.0
128-129	57106.0
130-131	86301.0
132-133	64829.0
134-135	83617.0
136-137	88397.0
138-139	88570.0
140-141	86676.0
142-143	88631.0
144-145	92349.0
146-147	77726.0
148-149	75926.0
150-151	79408.0
152-153	75285.0
154-155	77698.0
156-157	88695.0
158-159	73035.0
160-161	72918.0
162-163	77954.0
164-165	75490.0
166-167	75789.0
168-169	74589.0
170-171	86058.0
172-173	2372764.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.88874304258526
#Duplication Level	Percentage of total
1	92.13716284793442
2	6.955002905076651
3	0.6592282271333382
4	0.15092676108677672
5	0.049139344159371844
6	0.019032809234870773
7	0.01283191053557228
8	0.00865088529661833
9	0.0032804477213639605
>10	0.00474386182100669
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	2.273227899507869E-5	0.0	0.0	0.0	0.0	0.0
4	2.273227899507869E-5	0.0	0.0	0.0	0.0	0.0
5	2.273227899507869E-5	0.0	0.0	0.0	0.0	0.0
6	4.546455799015738E-5	0.0	2.273227899507869E-5	2.273227899507869E-5	0.0	0.0
7	9.092911598031476E-5	0.0	2.273227899507869E-5	2.273227899507869E-5	0.0	0.0
8	9.092911598031476E-5	2.273227899507869E-5	2.273227899507869E-5	2.273227899507869E-5	0.0	0.0
9	9.092911598031476E-5	2.273227899507869E-5	2.273227899507869E-5	2.273227899507869E-5	0.0	0.0
10-14	9.092911598031476E-5	2.7278734794094425E-5	4.091810219114164E-5	2.273227899507869E-5	0.0	0.0
15-19	1.1820785077440918E-4	5.001101378917311E-5	4.546455799015738E-5	4.546455799015737E-5	1.818582319606295E-5	0.0
20-24	1.5912595296555082E-4	6.819683698523607E-5	4.546455799015738E-5	9.092911598031476E-5	6.365038118622033E-5	0.0
25-29	1.7731177616161378E-4	6.819683698523607E-5	4.546455799015738E-5	9.092911598031476E-5	1.091149391763777E-4	0.0
30-34	4.273668451074793E-4	6.819683698523607E-5	5.910392538720459E-5	9.092911598031476E-5	1.1366139497539344E-4	0.0
35-39	0.0011547997729499976	7.728974858326754E-5	1.091149391763777E-4	1.0456848337736196E-4	1.3639367397047213E-4	2.7278734794094425E-5
40-44	0.0022414027089147585	1.2275430657342491E-4	1.318472181714564E-4	1.454865855685036E-4	2.0004405515669244E-4	6.365038118622033E-5
45-49	0.0030961363991297173	1.682188645635823E-4	2.0913696675472392E-4	1.7731177616161378E-4	3.000660827350387E-4	1.3639367397047213E-4
50-54	0.0038190228711732195	2.318692457498026E-4	2.318692457498026E-4	2.0459051095570818E-4	3.591700081222433E-4	1.3639367397047213E-4
55-59	0.004473712506231487	2.682408921419285E-4	2.682408921419285E-4	2.0459051095570818E-4	4.0918102191141636E-4	1.3639367397047213E-4
60-64	0.005119309229691721	2.8188025953897574E-4	2.9551962693602295E-4	2.0459051095570818E-4	4.410062125045266E-4	1.6367240876456656E-4
65-69	0.00801994802946376	3.0461253853405443E-4	3.364377291271646E-4	2.182298783527554E-4	4.910172262936997E-4	2.0459051095570818E-4
70-74	0.06204548228916777	3.591700081222433E-4	3.5462355232322756E-4	2.5005506894586557E-4	5.955857096710616E-4	2.2277633415177114E-4
75-79	0.08563704143026045	4.000881103133849E-4	4.6828494729862097E-4	2.5005506894586557E-4	7.910833090287384E-4	2.2732278995078687E-4
80-84	0.08586436422021122	4.0918102191141636E-4	5.228424168868098E-4	2.5005506894586557E-4	0.0011138816707588557	2.2732278995078687E-4
85-89	0.08623262713993149	4.319133009064951E-4	5.273888726858256E-4	2.5005506894586557E-4	0.0011366139497539344	2.2732278995078687E-4
90-94	0.08651450739947046	4.410062125045266E-4	5.455746958818885E-4	2.7278734794094426E-4	0.0011366139497539344	2.2732278995078687E-4
95-99	0.08677365538001436	4.7737785889665244E-4	5.955857096710616E-4	2.9551962693602295E-4	0.0011547997729499974	2.2732278995078687E-4
100-104	0.08716919703452873	5.046565936907469E-4	6.41050267661219E-4	2.9551962693602295E-4	0.0011820785077440918	2.2732278995078687E-4
105-109	0.0874465308382687	5.455746958818884E-4	6.819683698523607E-4	3.1825190593110165E-4	0.0011820785077440918	2.2732278995078687E-4
110-114	0.0877647827441998	5.955857096710616E-4	7.274329278425181E-4	3.4098418492618034E-4	0.0012229966099352335	2.2732278995078687E-4
115-119	0.0880830346501309	6.137715328671246E-4	7.728974858326755E-4	3.5462355232322756E-4	0.001268461167925391	2.2732278995078687E-4
120-124	0.08875136365258621	6.546896350582661E-4	8.638266018129902E-4	3.6371646392125903E-4	0.0012957399027194853	2.2732278995078687E-4
125-129	0.09021077596407026	6.59236090857282E-4	8.86558880808069E-4	3.6826291972027477E-4	0.0013593902839057054	2.2732278995078687E-4
130-134	0.09176566384733365	6.59236090857282E-4	8.956517924061004E-4	3.864487429163377E-4	0.0014548658556850361	2.2732278995078687E-4
135-139	0.09297956754567085	6.865148256513764E-4	9.729415409893678E-4	3.864487429163377E-4	0.0015003304136751935	2.2732278995078687E-4
140-144	0.09449808378254211	7.183400162444866E-4	9.774879967883836E-4	3.955416545143692E-4	0.001545794971665351	2.2732278995078687E-4
145-149	0.09608025040059959	7.410722952395653E-4	9.774879967883836E-4	4.0918102191141636E-4	0.0016185382644496027	2.2732278995078687E-4
150-154	0.09722141080615254	7.63804574234644E-4	9.774879967883836E-4	4.2282038930846363E-4	0.0016730957340377914	2.2732278995078687E-4
155-159	0.0981397948775537	7.728974858326755E-4	9.774879967883836E-4	4.319133009064951E-4	0.0017094673804299173	2.2732278995078687E-4
160-162	0.09881721679160704	7.728974858326755E-4	9.774879967883836E-4	4.3949072723818797E-4	0.0017352306299576734	2.273227899507869E-4
>>END_MODULE
