##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293558
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473779
Total Bases	81.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3558009113954	39.0	39.0	39.0	37.0	39.0
2	40.12559653340482	41.0	40.0	41.0	38.0	41.0
3	40.15344707131384	41.0	40.0	41.0	38.0	41.0
4	40.17119163154129	41.0	40.0	41.0	38.0	41.0
5	40.17076949379352	41.0	40.0	41.0	38.0	41.0
6	40.166691643150074	41.0	40.0	41.0	38.0	41.0
7	40.19516061285958	41.0	40.0	41.0	38.0	41.0
8	40.17703612865914	41.0	40.0	41.0	38.0	41.0
9	40.16116586003179	41.0	40.0	41.0	38.0	41.0
10-14	40.104953153263445	41.0	40.0	41.0	38.0	41.0
15-19	39.99276455900325	41.0	40.0	41.0	38.0	41.0
20-24	39.832098510064824	41.0	40.0	41.0	37.8	41.0
25-29	39.60791930414814	40.6	39.2	41.0	36.8	41.0
30-34	39.412547622414664	40.0	39.0	41.0	36.0	41.0
35-39	39.311668098417194	40.0	38.8	41.0	35.0	41.0
40-44	38.91633736404526	40.0	37.4	41.0	35.0	41.0
45-49	38.36456195821258	39.6	35.6	41.0	35.0	41.0
50-54	37.78452316375356	38.6	35.0	41.0	34.8	41.0
55-59	37.203601257126216	37.0	35.0	40.0	34.0	41.0
60-64	36.45482007433846	35.8	34.8	38.8	33.6	40.8
65-69	36.03693240941452	35.0	35.0	37.4	33.8	40.0
70-74	35.70576830125439	35.0	35.0	36.6	34.0	39.0
75-79	35.3957431629515	35.0	35.0	36.0	34.0	38.6
80-84	35.2163063369208	35.0	35.0	35.6	33.2	38.0
85-89	34.944609195426565	35.0	34.6	35.0	32.6	37.2
90-94	39.31885321707787	40.2	39.6	40.2	37.6	40.2
95-99	39.90063401801412	41.0	40.0	41.0	38.0	41.0
100-104	39.80599825834082	41.0	40.0	41.0	37.2	41.0
105-109	39.6731430906859	41.0	39.6	41.0	36.8	41.0
110-114	39.512924487412555	41.0	39.0	41.0	36.0	41.0
115-119	39.28141030934796	40.0	38.6	41.0	35.2	41.0
120-124	39.01019461461518	40.0	38.0	41.0	35.0	41.0
125-129	38.678467152122344	39.8	37.4	40.8	34.8	41.0
130-134	38.315751559039825	39.8	36.4	40.8	34.2	41.0
135-139	38.1330045217065	39.0	35.2	41.0	34.6	41.0
140-144	37.74883468121392	38.4	35.0	41.0	34.4	41.0
145-149	37.18255512276352	36.8	35.0	40.2	34.0	41.0
150-154	36.559159518929825	35.8	35.0	39.0	34.0	41.0
155-159	35.943743706189025	35.0	35.0	37.2	34.0	39.4
160-164	35.43472278056296	35.0	35.0	36.2	34.0	38.4
165-169	35.06027169286388	35.0	35.0	35.6	33.2	37.0
170-174	34.80965965203863	35.0	35.0	35.0	33.0	36.0
175-179	34.45255755400615	35.0	34.6	35.0	32.4	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	3.0
30	27.0
31	191.0
32	941.0
33	3531.0
34	9929.0
35	24793.0
36	63259.0
37	127917.0
38	147205.0
39	91297.0
40	4685.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.098866771216116	27.793549313076348	29.14037979733167	21.967204118375864
2	22.218165009424226	28.952106361826928	28.463904056532684	20.36582457221616
3	19.11988500967751	27.779407698526104	31.541921444386517	21.558785847409865
4	20.49837582501546	25.827442752844682	31.970180189497633	21.704001232642224
5	21.836974623189292	26.567872362430585	29.259422642202377	22.335730372177746
6	21.939131958149265	27.52865787635163	28.072793433225197	22.45941673227391
7	21.89459642575969	28.12218354971411	27.88557534208988	22.097644682436325
8	21.58875762750143	28.393407052655352	28.30349171238067	21.714343607462553
9	21.775975718636747	27.84568332492576	28.551075501446878	21.82726545499062
10-14	22.07105000432691	27.983004734274843	27.83990003778133	22.106045223616917
15-19	22.0645068692365	27.89308939400016	28.0180843811144	22.02431935564894
20-24	21.94787020952807	27.813220932122363	28.123154466533972	22.115754391815592
25-29	22.01321713288263	27.73141063660483	27.938258133011384	22.317114097501157
30-34	22.074722602732496	27.82660269872662	27.89731077147784	22.20136392706304
35-39	22.1467393025018	27.821283763104738	27.82111490800563	22.210862026387833
40-44	22.113939199500187	27.75741432186737	27.84361484996169	22.28503162867075
45-49	22.202756981630674	27.70717992988292	27.87147594131441	22.218587147171995
50-54	22.171814284719247	27.735083235010414	27.88063633044099	22.212466149829353
55-59	22.148132357069436	27.7934648855268	27.77202028794016	22.28638246946361
60-64	22.096884834490343	27.832554840970158	27.867254563836724	22.203305760702776
65-69	22.048972200118623	27.77746586488637	27.876752663161515	22.296809271833492
70-74	22.0564862520289	27.70772870895502	27.866579143440294	22.369205895575785
75-79	22.133019825699325	27.710599245639845	27.879623199846343	22.276757728814488
80-84	22.162738323142225	27.822423535023717	27.735463158983407	22.27937498285065
85-89	22.24986755428164	27.766279214570506	27.683666857332216	22.300186373815638
90-94	20.97009603770072	27.887960097088975	29.853931668713628	21.288012196496673
95-99	21.769123223429848	27.85933334347671	28.294061560152763	22.077481872940684
100-104	21.974180247190585	28.09099356728736	27.85226986488975	22.082556320632307
105-109	22.069339212731002	27.96897701196247	27.94254178717395	22.019141988132578
110-114	21.931075695684985	27.953429040961552	28.047749108620028	22.067746154733435
115-119	21.936502565837703	27.897568136106454	27.907693070102134	22.258236227953702
120-124	22.08442718216675	27.91962715539525	27.757410749872257	22.238534912565743
125-129	22.03347605669741	27.998806951061717	27.785799441922048	22.181917550318822
130-134	22.11437282611097	27.88647192837522	27.772018525682483	22.227136719831325
135-139	22.19717109124928	27.89668991871544	27.75582134218382	22.150317647851455
140-144	22.049523452127133	28.055321139276646	27.803685099078024	22.091470309518193
145-149	22.063898871081427	27.97453289116393	27.792426886056642	22.169141351698
150-154	22.037313170422028	28.13004583602999	27.873542736419544	21.95909825712844
155-159	21.93650685232257	28.142676625993452	27.93659467133734	21.984221850346643
160-164	21.962233662921708	28.083626033472065	27.932063495882147	22.022076807724076
165-169	21.947716454897463	28.25650889358475	27.901569722992793	21.894204928524992
170-174	21.9847331502108	28.33510889993646	27.876217845751363	21.803940104101372
175-179	21.95723752853994	28.366600020144205	27.936560226995127	21.73960222432073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	3.5
11	6.5
12	8.5
13	20.0
14	28.0
15	49.0
16	97.0
17	148.5
18	248.0
19	450.5
20	717.0
21	1046.5
22	1420.0
23	1840.0
24	2289.0
25	2753.5
26	3264.0
27	3910.0
28	4678.0
29	5490.5
30	6484.0
31	7631.5
32	9009.0
33	10577.0
34	12375.0
35	14067.0
36	15909.0
37	17925.0
38	19336.5
39	20155.0
40	20333.0
41	19872.0
42	18772.5
43	17490.0
44	16119.0
45	14731.5
46	13747.0
47	13101.5
48	12841.0
49	12739.0
50	12725.5
51	12912.0
52	12851.0
53	12623.0
54	12311.0
55	11585.5
56	10770.0
57	9736.5
58	8588.0
59	7645.0
60	6605.5
61	5699.5
62	4939.5
63	4260.0
64	3667.0
65	3057.0
66	2603.0
67	2218.5
68	1823.5
69	1474.5
70	1162.0
71	903.5
72	663.5
73	446.0
74	310.5
75	221.0
76	142.5
77	79.0
78	43.5
79	31.0
80	14.5
81	6.5
82	6.0
83	3.0
84	1.5
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	63.0
92-93	104.0
94-95	214.0
96-97	347.0
98-99	272.0
100-101	340.0
102-103	390.0
104-105	433.0
106-107	535.0
108-109	581.0
110-111	610.0
112-113	625.0
114-115	755.0
116-117	717.0
118-119	798.0
120-121	811.0
122-123	839.0
124-125	893.0
126-127	876.0
128-129	896.0
130-131	1000.0
132-133	1060.0
134-135	4405.0
136-137	4207.0
138-139	4356.0
140-141	4378.0
142-143	3981.0
144-145	4316.0
146-147	4474.0
148-149	4806.0
150-151	4989.0
152-153	4239.0
154-155	4342.0
156-157	4300.0
158-159	4384.0
160-161	4257.0
162-163	4600.0
164-165	4390.0
166-167	4447.0
168-169	5860.0
170-171	4820.0
172-173	5467.0
174-175	5251.0
176-177	6218.0
178-179	358133.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.2147269202505
#Duplication Level	Percentage of total
1	93.32765890584473
2	4.792737688170771
3	0.8674934212094341
4	0.39818903239952796
5	0.2552579789584414
6	0.17095669095584834
7	0.06174494201855047
8	0.04946725525503523
9	0.03435618244886114
>10	0.04213790273880233
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.1106887388423716E-4	0.0	0.0	0.0	0.0	0.0
2	4.221377477684743E-4	0.0	0.0	0.0	0.0	0.0
3	4.221377477684743E-4	0.0	0.0	0.0	0.0	0.0
4	4.221377477684743E-4	0.0	0.0	0.0	2.1106887388423716E-4	0.0
5	4.221377477684743E-4	0.0	0.0	0.0	4.221377477684743E-4	0.0
6	4.221377477684743E-4	0.0	0.0	0.0	6.332066216527115E-4	0.0
7	4.221377477684743E-4	0.0	0.0	0.0	6.332066216527115E-4	0.0
8	4.221377477684743E-4	0.0	0.0	0.0	8.442754955369486E-4	0.0
9	4.221377477684743E-4	0.0	0.0	0.0	8.442754955369486E-4	0.0
10-14	4.2213774776847437E-4	0.0	0.0	0.0	0.0016885509910738975	0.0
15-19	4.2213774776847437E-4	0.0	0.0	2.1106887388423718E-4	0.002448398937057151	0.0
20-24	4.2213774776847437E-4	0.0	0.0	2.1106887388423718E-4	0.0034193157569246424	0.0
25-29	4.2213774776847437E-4	0.0	0.0	4.2213774776847437E-4	0.005023439198444844	0.0
30-34	6.754203964295589E-4	0.0	0.0	7.176341712064063E-4	0.007302983036394606	0.0
35-39	0.0011397719189748807	0.0	0.0	8.442754955369487E-4	0.010131305946443384	4.221377477684743E-5
40-44	0.0027438953604950827	0.0	0.0	8.442754955369487E-4	0.013635049252921722	2.1106887388423718E-4
45-49	0.004348018802015286	0.0	0.0	8.442754955369487E-4	0.016421158388193653	2.1106887388423718E-4
50-54	0.005614432045320709	0.0	0.0	8.442754955369487E-4	0.019333908847796122	2.1106887388423718E-4
55-59	0.006669776414741894	0.0	0.0	0.0010553443694211859	0.02220444553262175	2.1106887388423718E-4
60-64	0.007471838135501996	0.0	0.0	0.001266413243305423	0.024441775595794663	3.3771019821477946E-4
65-69	0.008316113631038945	0.0	0.0	0.001266413243305423	0.027270098505843443	4.2213774776847437E-4
70-74	0.018405205802705483	0.0	0.0	0.001266413243305423	0.029507428569016354	4.2213774776847437E-4
75-79	0.18194136928821245	0.0	0.0	0.001266413243305423	0.031364834659197635	4.2213774776847437E-4
80-84	0.24555752787692148	0.0	0.0	0.001266413243305423	0.03187139995651981	4.2213774776847437E-4
85-89	0.24610630694902053	0.0	0.0	0.001266413243305423	0.0320402550556272	4.2213774776847437E-4
90-94	0.2462329482733511	0.0	0.0	0.001266413243305423	0.03212468260518089	4.2213774776847437E-4
95-99	0.24648623092201216	0.0	0.0	0.001266413243305423	0.032504606578172515	4.2213774776847437E-4
100-104	0.24652844469678897	0.0	0.0	0.001266413243305423	0.03271567545205676	4.2213774776847437E-4
105-109	0.24652844469678897	0.0	0.0	0.001266413243305423	0.033306668298932626	4.2213774776847437E-4
110-114	0.24652844469678897	0.0	0.0	0.001435268342412813	0.03474193664134544	4.2213774776847437E-4
115-119	0.24657065847156584	0.0	0.0	0.0014774821171896601	0.03583949478554347	4.2213774776847437E-4
120-124	0.24695058244455748	0.0	0.0	0.0014774821171896601	0.03833010749737747	4.643515225453218E-4
125-129	0.24707722376888802	0.0	0.0	0.0015619096667433548	0.04153835438041788	6.332066216527115E-4
130-134	0.24804814058875552	0.0	0.0	0.0016885509910738975	0.04495767013734252	6.332066216527115E-4
135-139	0.2491879125077304	0.0	0.0	0.0018151923154044395	0.04820813079515977	6.332066216527115E-4
140-144	0.2496522640302757	0.0	0.0	0.0018996198649581346	0.050487674633109526	6.332066216527115E-4
145-149	0.24998997422849048	0.0	0.0	0.0018996198649581346	0.05428691436302581	6.332066216527115E-4
150-154	0.2502432568771516	0.0	0.0	0.0018996198649581346	0.056819740849636646	6.332066216527115E-4
155-159	0.2521850905168866	0.0	0.0	0.0021106887388423717	0.05947920866057803	6.332066216527115E-4
160-164	0.25366257263407627	0.0	0.0	0.0021106887388423717	0.061843180048081495	6.332066216527115E-4
165-168	0.2558682423661665	0.0	0.0	0.0021106887388423717	0.06400663600539491	6.332066216527115E-4
>>END_MODULE
