##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293559
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481005
Total Bases	82.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.34129374954522	39.0	39.0	39.0	37.0	39.0
2	40.11013606927163	41.0	40.0	41.0	38.0	41.0
3	40.14296940780241	41.0	40.0	41.0	38.0	41.0
4	40.163526366669785	41.0	40.0	41.0	38.0	41.0
5	40.16002536356171	41.0	40.0	41.0	38.0	41.0
6	40.15397968836083	41.0	40.0	41.0	38.0	41.0
7	40.1814575732061	41.0	40.0	41.0	38.0	41.0
8	40.16553050384092	41.0	40.0	41.0	38.0	41.0
9	40.146547333187804	41.0	40.0	41.0	38.0	41.0
10-14	40.086210330453945	41.0	40.0	41.0	38.0	41.0
15-19	39.97359944283324	41.0	40.0	41.0	38.0	41.0
20-24	39.80941694992775	41.0	40.0	41.0	37.8	41.0
25-29	39.58999012484278	40.4	39.2	41.0	36.8	41.0
30-34	39.41020529932121	40.0	39.0	41.0	36.0	41.0
35-39	39.32047317595452	40.0	38.8	41.0	35.0	41.0
40-44	38.92921528882236	40.0	37.4	41.0	35.0	41.0
45-49	38.381452167856885	39.6	36.0	41.0	35.0	41.0
50-54	37.80441034916477	38.6	35.0	41.0	34.6	41.0
55-59	37.2313518570493	37.0	35.0	40.0	34.0	41.0
60-64	36.48507229654577	35.8	34.8	38.8	33.6	40.8
65-69	36.048866020103745	35.0	35.0	37.4	33.8	40.0
70-74	35.69967755012941	35.0	35.0	36.6	34.0	39.0
75-79	35.384495795262005	35.0	35.0	36.0	33.8	38.8
80-84	35.19596012515463	35.0	35.0	35.6	33.0	37.8
85-89	34.917014584047976	35.0	34.6	35.0	32.6	37.2
90-94	39.60696317808703	40.6	39.8	40.6	37.8	40.6
95-99	39.88250054975754	41.0	40.0	41.0	38.0	41.0
100-104	39.76952629979014	41.0	40.0	41.0	37.0	41.0
105-109	39.624525649964674	41.0	39.4	41.0	36.6	41.0
110-114	39.451564213462596	40.4	39.0	41.0	36.0	41.0
115-119	39.19220748228562	40.0	38.4	41.0	35.0	41.0
120-124	38.95865613774055	40.0	38.0	41.0	35.0	41.0
125-129	38.493573364172946	39.6	37.2	40.6	34.6	41.0
130-134	38.294611864388884	39.8	36.2	41.0	34.0	41.0
135-139	38.084561425414975	39.0	35.0	41.0	34.4	41.0
140-144	37.66734167329529	38.2	35.0	41.0	34.0	41.0
145-149	37.08650490393806	36.8	35.0	39.8	34.0	41.0
150-154	36.45139624083426	35.8	35.0	38.8	34.0	40.8
155-159	35.836433132723606	35.0	35.0	37.0	34.0	39.2
160-164	35.33489053214172	35.0	35.0	36.0	33.2	38.0
165-169	34.968781368596765	35.0	35.0	35.4	33.0	36.8
170-174	34.723978497525806	35.0	35.0	35.0	33.0	36.0
175-178	34.321819823169065	34.75	34.25	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	18.0
31	203.0
32	933.0
33	3664.0
34	10439.0
35	25759.0
36	65814.0
37	128235.0
38	149057.0
39	92310.0
40	4570.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.928264778952403	27.966445255246825	29.287013648506772	21.818276317294
2	22.031163917214997	29.22485213251422	28.531096350349788	20.212887599920997
3	18.997099822247172	27.981413914616272	31.53688631095311	21.48459995218345
4	20.457167804908472	25.91386783921165	32.06369996153886	21.565264394341018
5	21.832413384476254	26.71677009594495	29.117368842319724	22.333447677259073
6	21.958815396929346	27.7080279830771	28.09347096184031	22.239685658153242
7	21.779815178636397	28.226941507884533	27.941497489631082	22.051745823847984
8	21.425972702986456	28.677872371389068	28.18473820438457	21.711416721239903
9	21.723059011860585	27.889315079884824	28.55978628080789	21.8278396274467
10-14	21.928919657799813	28.124821987297427	27.896258874647874	22.049999480254883
15-19	21.948254176152016	28.01446970405713	28.096007318011246	21.941268801779607
20-24	21.852330017359485	27.94977183189364	28.153096121661935	22.044802029084938
25-29	21.88505316992547	27.869065810126713	28.04444860240538	22.20143241754244
30-34	21.989875365120945	27.881207056059708	27.966445255246825	22.162472323572523
35-39	21.989875365120945	27.927734638933067	27.905198490660183	22.177191505285805
40-44	21.99698547832143	27.862288333801104	27.94299435556803	22.197731832309437
45-49	22.130289705928213	27.843951726073534	27.89742310370994	22.128335464288313
50-54	22.060976497125807	27.922121391669524	27.901539485036537	22.115362626168128
55-59	22.003679795428322	27.872558497312916	27.890312990509454	22.233448716749308
60-64	22.047754181349465	27.915634972609432	27.875261171921288	22.161349674119812
65-69	21.968669764347563	27.923535098387752	27.963285204935502	22.144509932329186
70-74	22.015821041361317	27.743994345173128	27.968940031808405	22.271244581657154
75-79	22.048377875489862	27.847943368572054	27.955468238375904	22.148210517562188
80-84	22.065176037671126	27.9263625118242	27.818380266317398	22.190081184187274
85-89	22.079812060165697	27.86944002661095	27.821373998191284	22.22937391503207
90-94	20.882121896519244	27.714468334442977	29.894218729402137	21.509191039635645
95-99	21.66263310241785	28.182440573425087	28.176489071919526	21.978437252237534
100-104	21.89231127673577	28.167477817183332	27.94103365736285	21.999177248718045
105-109	21.82533184509885	28.049933831490865	28.12370503549038	22.001029287919902
110-114	21.81187302459805	28.00985899442614	28.156830575451558	22.021437405524257
115-119	21.889467075652412	28.01529955319554	27.938036725485343	22.1571966456667
120-124	21.922121898067733	28.05487894425004	27.868058263407452	22.154940894274773
125-129	21.89520487269367	28.062409135029053	27.91210840641385	22.13027758586342
130-134	21.88295523089978	28.05208237487178	27.918281141906835	22.146681252321606
135-139	22.024274050334363	28.004774341685394	27.84149636504897	22.12945524293127
140-144	21.980028013395238	28.087117037690103	27.86017476377029	22.07268018514437
145-149	21.93414365486186	28.135730753345843	27.90336248675483	22.02676310503747
150-154	21.88155714558219	28.227658484221706	27.926006410807858	21.964777959388243
155-159	21.813796763627323	28.18836260963618	28.101027957645574	21.896812669090924
160-164	21.831933901001513	28.31348665900828	28.010111836157858	21.844467603832353
165-169	21.854344062844426	28.328433153507422	28.043327155951914	21.773895627696245
170-174	21.801790329107558	28.582960457733193	27.969274664648765	21.645974548510488
175-178	21.75665333530835	28.66914092288111	28.044130636339197	21.530075105471344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	1.0
10	4.0
11	7.5
12	11.0
13	15.0
14	22.0
15	40.0
16	82.5
17	159.5
18	274.5
19	445.0
20	721.0
21	1132.5
22	1567.0
23	1980.0
24	2396.0
25	2857.0
26	3344.5
27	3970.5
28	4823.0
29	5668.0
30	6630.5
31	7945.0
32	9374.0
33	10869.0
34	12607.5
35	14463.0
36	16458.5
37	18375.5
38	19939.0
39	20821.0
40	20887.0
41	20508.0
42	19535.5
43	18015.0
44	16433.5
45	15118.0
46	13954.0
47	12941.5
48	12643.0
49	12800.0
50	12779.5
51	12787.5
52	12795.5
53	12582.0
54	12152.5
55	11540.0
56	10739.0
57	9762.0
58	8700.5
59	7553.0
60	6521.5
61	5636.5
62	4889.5
63	4229.5
64	3592.0
65	3050.0
66	2585.0
67	2191.0
68	1764.0
69	1425.5
70	1164.5
71	876.0
72	650.5
73	456.0
74	300.0
75	193.0
76	114.5
77	72.0
78	49.0
79	30.0
80	14.5
81	5.5
82	4.0
83	3.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	53.0
92-93	116.0
94-95	171.0
96-97	216.0
98-99	264.0
100-101	301.0
102-103	404.0
104-105	436.0
106-107	522.0
108-109	575.0
110-111	568.0
112-113	597.0
114-115	665.0
116-117	687.0
118-119	739.0
120-121	743.0
122-123	813.0
124-125	828.0
126-127	878.0
128-129	940.0
130-131	945.0
132-133	1132.0
134-135	4825.0
136-137	4315.0
138-139	4849.0
140-141	4266.0
142-143	4386.0
144-145	4284.0
146-147	4509.0
148-149	4640.0
150-151	4640.0
152-153	4415.0
154-155	4323.0
156-157	4334.0
158-159	4487.0
160-161	4758.0
162-163	4848.0
164-165	4952.0
166-167	5049.0
168-169	5143.0
170-171	5189.0
172-173	5738.0
174-175	5787.0
176-177	15840.0
178-179	352835.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.8020917487235
#Duplication Level	Percentage of total
1	92.6605608947992
2	5.095753762830241
3	1.071607230076458
4	0.5021912538428888
5	0.2865936360942527
6	0.15648052427577694
7	0.08822744052293913
8	0.05488642611736618
9	0.03826252264119075
>10	0.04543630879966647
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0789804679785032E-4	0.0	0.0	2.0789804679785032E-4	4.1579609359570065E-4	0.0
2	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	8.315921871914013E-4	0.0
3	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	8.315921871914013E-4	0.0
4	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	8.315921871914013E-4	0.0
5	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	8.315921871914013E-4	0.0
6	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	0.0010394902339892517	0.0
7	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	0.0010394902339892517	0.0
8	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	0.0010394902339892517	0.0
9	8.315921871914013E-4	0.0	0.0	2.0789804679785032E-4	0.001247388280787102	0.0
10-14	8.315921871914014E-4	0.0	4.157960935957006E-5	2.0789804679785035E-4	0.0016216047650232327	0.0
15-19	8.315921871914014E-4	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.0023700377334954937	0.0
20-24	8.315921871914014E-4	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.0027026746083720545	0.0
25-29	8.315921871914014E-4	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.003617426014282596	0.0
30-34	0.0011642290620679618	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.0049479735137888375	0.0
35-39	0.0023284581241359236	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.007151692809846051	2.494776561574204E-4
40-44	0.003451107576844316	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.010519641167971227	4.157960935957007E-4
45-49	0.004989553123148408	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.013970748744815542	4.157960935957007E-4
50-54	0.00640325984137379	0.0	2.0789804679785035E-4	2.0789804679785035E-4	0.01733869710294072	4.989553123148408E-4
55-59	0.0074843296847226124	0.0	2.0789804679785035E-4	2.494776561574204E-4	0.02091454350786374	6.23694140393551E-4
60-64	0.008814877184228853	0.0	2.0789804679785035E-4	4.157960935957007E-4	0.023284581241359237	6.23694140393551E-4
65-69	0.009812787808858536	0.0	2.494776561574204E-4	4.157960935957007E-4	0.025072504443820753	6.23694140393551E-4
70-74	0.021413498820178584	0.0	4.157960935957007E-4	4.157960935957007E-4	0.02715148491179925	6.23694140393551E-4
75-79	0.18423924907225497	0.0	4.157960935957007E-4	4.157960935957007E-4	0.02873151006746292	6.23694140393551E-4
80-84	0.24540285444018256	0.0	4.157960935957007E-4	4.157960935957007E-4	0.029521522645294746	6.23694140393551E-4
85-89	0.24573549131505906	0.0	4.157960935957007E-4	4.157960935957007E-4	0.0297294206920926	7.900125778318312E-4
90-94	0.24590180975249737	0.0	4.157960935957007E-4	4.157960935957007E-4	0.02993731873889045	8.315921871914014E-4
95-99	0.24594338936185695	0.0	4.157960935957007E-4	6.23694140393551E-4	0.03035311483248615	8.315921871914014E-4
100-104	0.24648392428353133	0.0	4.157960935957007E-4	6.23694140393551E-4	0.0309768089728797	8.315921871914014E-4
105-109	0.24656708350225048	0.0	4.157960935957007E-4	6.23694140393551E-4	0.031974719597509374	8.315921871914014E-4
110-114	0.24665024272096964	0.0	4.157960935957007E-4	6.23694140393551E-4	0.03280631178470079	8.315921871914014E-4
115-119	0.24694129998648662	0.0	4.157960935957007E-4	6.23694140393551E-4	0.034261598112285734	8.315921871914014E-4
120-124	0.24735709608008233	0.0	4.157960935957007E-4	6.23694140393551E-4	0.03683953389257909	8.315921871914014E-4
125-129	0.24793921061111632	0.0	4.157960935957007E-4	6.23694140393551E-4	0.038918514360557585	8.315921871914014E-4
130-134	0.24881238240766726	0.0	4.157960935957007E-4	6.23694140393551E-4	0.04087275600045737	0.0010394902339892517
135-139	0.24906186006382472	0.0	4.157960935957007E-4	6.23694140393551E-4	0.04274383842163802	0.001247388280787102
140-144	0.24943607654806083	0.0	4.157960935957007E-4	6.23694140393551E-4	0.04469808006153782	0.001288967890146672
145-149	0.2499350318603757	0.0	4.157960935957007E-4	6.23694140393551E-4	0.046527582873358904	0.0014552863275849523
150-154	0.25051714639140965	0.0	4.157960935957007E-4	6.23694140393551E-4	0.04810760802902257	0.0014552863275849523
155-159	0.2520140123283542	0.0	4.157960935957007E-4	6.23694140393551E-4	0.04960447396596709	0.0014552863275849523
160-164	0.2542177316244114	0.0	4.157960935957007E-4	6.23694140393551E-4	0.05089344185611376	0.0014552863275849523
165-167	0.2562689923528168	0.0	4.1579609359570065E-4	6.23694140393551E-4	0.051835913001597345	0.0014552863275849523
>>END_MODULE
