##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293560
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476100
Total Bases	81.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.32874186095358	39.0	39.0	39.0	37.0	39.0
2	40.11033186305398	41.0	40.0	41.0	38.0	41.0
3	40.14621718126444	41.0	40.0	41.0	38.0	41.0
4	40.17282503675698	41.0	40.0	41.0	38.0	41.0
5	40.18366519638732	41.0	40.0	41.0	38.0	41.0
6	40.16603234614577	41.0	40.0	41.0	38.0	41.0
7	40.19574039067423	41.0	40.0	41.0	38.0	41.0
8	40.18517538332283	41.0	40.0	41.0	38.0	41.0
9	40.170006301197226	41.0	40.0	41.0	38.0	41.0
10-14	40.114558286074356	41.0	40.0	41.0	38.0	41.0
15-19	40.00186599453897	41.0	40.0	41.0	38.0	41.0
20-24	39.837041377861794	41.0	40.0	41.0	37.8	41.0
25-29	39.61853770216341	40.6	39.2	41.0	36.8	41.0
30-34	39.4367893299727	40.0	39.0	41.0	36.0	41.0
35-39	39.33714051669818	40.4	38.8	41.0	35.0	41.0
40-44	38.93613064482251	40.0	37.4	41.0	35.0	41.0
45-49	38.378952320940975	39.6	36.0	41.0	35.0	41.0
50-54	37.81508632640202	38.6	35.0	41.0	34.8	41.0
55-59	37.23055744591472	37.0	35.0	40.0	34.0	41.0
60-64	36.48679395085066	35.8	34.8	38.8	33.6	40.8
65-69	36.05137912203319	35.0	35.0	37.4	33.8	40.0
70-74	35.72087964713295	35.0	35.0	36.6	34.0	39.0
75-79	35.409582020583905	35.0	35.0	36.0	34.0	38.8
80-84	35.23475698382693	35.0	35.0	35.6	33.4	38.0
85-89	34.962124763705106	35.0	34.6	35.0	32.6	37.4
90-94	39.63988388216922	40.6	39.8	40.6	37.8	40.6
95-99	39.91991762249698	41.0	40.0	41.0	38.0	41.0
100-104	39.81142278392102	41.0	40.0	41.0	37.2	41.0
105-109	39.67337472662445	41.0	39.8	41.0	36.8	41.0
110-114	39.50483158398739	40.8	39.0	41.0	36.0	41.0
115-119	39.24382630250723	40.0	38.4	41.0	35.0	41.0
120-124	39.01177284961828	40.0	38.0	41.0	35.0	41.0
125-129	38.54247291203332	39.6	37.2	40.6	34.6	41.0
130-134	38.343864206526106	39.8	36.2	41.0	34.6	41.0
135-139	38.11106845513582	39.0	35.0	41.0	34.8	41.0
140-144	37.67512683919239	38.2	35.0	41.0	34.2	41.0
145-149	37.0914943686048	36.6	35.0	39.8	34.0	41.0
150-154	36.46671705770886	35.6	35.0	38.8	34.0	40.8
155-159	35.86236016180315	35.0	35.0	37.0	34.0	39.2
160-164	35.37092385554321	35.0	35.0	36.0	34.0	38.0
165-169	35.00892938490553	35.0	35.0	35.4	33.0	36.8
170-174	34.78073423691243	35.0	35.0	35.0	33.0	36.0
175-178	34.382115058130026	35.0	34.5	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	18.0
31	196.0
32	896.0
33	3388.0
34	9960.0
35	24233.0
36	63044.0
37	127448.0
38	147921.0
39	94105.0
40	4889.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.020373871035495	27.925855912623398	29.107960512497378	21.94580970384373
2	22.100609115731988	29.243016173072885	28.412098298676746	20.244276412518378
3	19.11825246796891	27.886788489813064	31.498004620877968	21.496954421340057
4	20.46208779668137	25.7296786389414	32.084646082755725	21.723587481621507
5	21.814324721697123	26.565217391304348	29.202688510817055	22.417769376181475
6	21.877336693971856	27.624658685150177	28.078764965343417	22.41923965553455
7	21.868094938038226	28.07036336904012	27.855702583490867	22.20583910943079
8	21.572358748162152	28.499894980046207	28.239655534551567	21.688090737240078
9	21.753203108590633	27.84162990968284	28.580550304557867	21.824616677168663
10-14	22.02978365889519	28.060449485402227	27.839991598403696	22.069775257298886
15-19	22.04066372610796	27.930644822516275	28.057971014492754	21.970720436883006
20-24	21.875026254988448	27.880823356437723	28.16244486452426	22.081705524049568
25-29	21.99966393614787	27.735097668557025	27.946565847511028	22.31867254778408
30-34	22.005923125393824	27.80907372400756	27.984289014912832	22.200714135685782
35-39	22.058223062381852	27.869271161520686	27.833606385213187	22.238899390884267
40-44	22.07616047048939	27.766267590842258	27.898970804452844	22.2586011342155
45-49	22.186431421970173	27.76601554295316	27.826969124133587	22.220583910943077
50-54	22.1103129594623	27.82898550724638	27.83600084015963	22.224700693131695
55-59	22.128754463348034	27.759504305818105	27.79399285864314	22.317748372190714
60-64	22.12257929006511	27.877336693971856	27.75979836168872	22.24028565427431
65-69	22.07305187985717	27.796429321571097	27.88195757193867	22.24856122663306
70-74	22.09556815795001	27.753035076664567	27.841083805923127	22.310312959462298
75-79	22.153665196387315	27.757319890779247	27.804746901911365	22.284268010922077
80-84	22.13921445074564	27.900651123713505	27.70405377021634	22.25608065532451
85-89	22.188489813064482	27.811678218861584	27.723545473639994	22.276286494433943
90-94	21.003156132779413	27.614565267078074	29.737980478360825	21.644298121781684
95-99	21.79268846503179	28.03356376358193	28.15281562216167	22.020932149224613
100-104	22.02622651164652	28.117457034976407	27.84714749306642	22.009168960310657
105-109	21.967922140527634	28.037227742164788	27.977750818282587	22.017099299024984
110-114	21.910818048073157	27.930483377610315	28.07955038579185	22.079148188524677
115-119	22.05208572738237	27.903286720446303	27.811015379831233	22.233612172340095
120-124	22.05136098903184	27.919324351463793	27.85621788445803	22.17309677504634
125-129	22.03815099834768	27.95737460228156	27.825454750977197	22.17901964839356
130-134	22.022592083649908	28.008384265047408	27.85694433808275	22.112079313219933
135-139	22.122460163874937	27.932058303024625	27.743749636142308	22.201731896958126
140-144	22.114767181491924	27.919131791949475	27.82184868376537	22.14425234279323
145-149	22.10480541044913	27.98997296244866	27.790267950950355	22.114953676151853
150-154	21.98816767942989	28.112778368340074	27.850446035738535	22.048607916491502
155-159	21.953482830550826	28.064897180908225	27.958782279441074	22.022837709099875
160-164	22.00118357319754	28.157703669076913	27.915717653143428	21.92539510458212
165-169	21.970465146291552	28.183016537305726	27.94349907511538	21.903019241287343
170-174	21.969019698136577	28.395645925796543	27.91535332523495	21.719981050831937
175-178	21.95918660125377	28.578138546501165	27.82910432474151	21.633570527503558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.5
9	2.0
10	4.5
11	12.5
12	15.5
13	23.5
14	40.5
15	51.0
16	77.5
17	130.0
18	236.0
19	461.0
20	726.0
21	1041.0
22	1448.0
23	1880.5
24	2251.0
25	2663.5
26	3236.0
27	3818.0
28	4539.5
29	5427.0
30	6416.5
31	7623.5
32	9047.0
33	10557.0
34	12333.0
35	14350.0
36	16198.5
37	17969.0
38	19576.5
39	20500.0
40	20839.0
41	20384.0
42	19214.5
43	17778.0
44	16187.5
45	14845.5
46	13827.5
47	13057.0
48	12627.0
49	12641.5
50	12758.5
51	12803.0
52	12751.0
53	12681.0
54	12232.0
55	11450.0
56	10730.5
57	9765.0
58	8657.5
59	7584.5
60	6633.5
61	5748.5
62	4944.0
63	4288.5
64	3694.0
65	3148.5
66	2686.5
67	2246.5
68	1835.5
69	1458.5
70	1158.5
71	911.0
72	643.0
73	456.5
74	309.0
75	199.0
76	126.5
77	69.0
78	40.0
79	26.5
80	14.5
81	11.0
82	7.5
83	3.5
84	2.0
85	1.5
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	57.0
92-93	132.0
94-95	169.0
96-97	215.0
98-99	316.0
100-101	319.0
102-103	400.0
104-105	428.0
106-107	521.0
108-109	546.0
110-111	544.0
112-113	673.0
114-115	646.0
116-117	685.0
118-119	676.0
120-121	709.0
122-123	744.0
124-125	802.0
126-127	832.0
128-129	882.0
130-131	905.0
132-133	1035.0
134-135	4726.0
136-137	4391.0
138-139	4184.0
140-141	4400.0
142-143	3885.0
144-145	4068.0
146-147	5357.0
148-149	4646.0
150-151	4341.0
152-153	4348.0
154-155	4411.0
156-157	4267.0
158-159	4215.0
160-161	4481.0
162-163	4391.0
164-165	4638.0
166-167	5003.0
168-169	5194.0
170-171	5053.0
172-173	5144.0
174-175	6114.0
176-177	15902.0
178-179	350705.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.93349675878258
#Duplication Level	Percentage of total
1	92.89715830538832
2	4.956622582245369
3	0.970716834944468
4	0.48613634004839495
5	0.26822243570233134
6	0.19203205142585264
7	0.08148901858755915
8	0.051289031288394106
9	0.02996446963488246
>10	0.0663689307344489
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.1003990758244065E-4	2.1003990758244065E-4	0.0
2	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	2.1003990758244065E-4	0.0
3	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	6.30119722747322E-4	0.0
4	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	6.30119722747322E-4	0.0
5	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	6.30119722747322E-4	0.0
6	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	6.30119722747322E-4	0.0
7	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	0.001260239445494644	0.0
8	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	0.001260239445494644	0.0
9	6.30119722747322E-4	0.0	0.0	2.1003990758244065E-4	0.0014702793530770846	0.0
10-14	6.30119722747322E-4	0.0	0.0	3.36063852131905E-4	0.001932367149758454	0.0
15-19	6.30119722747322E-4	0.0	0.0	4.200798151648813E-4	0.002646502835538752	0.0
20-24	6.30119722747322E-4	0.0	0.0	4.200798151648813E-4	0.0034446544843520265	0.0
25-29	6.30119722747322E-4	0.0	0.0	4.200798151648813E-4	0.004872925855912624	0.0
30-34	0.0010081915563957152	0.0	0.0	5.040957781978576E-4	0.00789750052509977	0.0
35-39	0.001932367149758454	0.0	0.0	6.30119722747322E-4	0.009745851711825246	0.0
40-44	0.0032766225582860744	0.0	0.0	6.30119722747322E-4	0.01306448225162781	0.0
45-49	0.004746901911363159	0.0	0.0	6.30119722747322E-4	0.015710985087166562	0.0
50-54	0.0058811174123083385	0.0	0.0	6.7212770426381E-4	0.017685360218441505	0.0
55-59	0.007477420709934887	0.0	0.0	8.401596303297626E-4	0.019953791220331862	0.0
60-64	0.00831758034026465	0.0	0.0	8.401596303297626E-4	0.02234824616677169	0.0
65-69	0.009367779878176854	0.0	0.0	8.401596303297626E-4	0.02474270111321151	0.0
70-74	0.01978575929426591	0.0	0.0	9.661835748792271E-4	0.02570888468809074	0.0
75-79	0.17992018483511868	4.200798151648813E-5	0.0	0.0010501995379122034	0.02659105229993699	1.680319260659525E-4
80-84	0.24574669187145556	2.9405587061541693E-4	0.0	0.0010501995379122034	0.02764125183784919	2.1003990758244065E-4
85-89	0.24616677168662043	4.200798151648813E-4	0.0	0.0010501995379122034	0.028229363579080024	2.1003990758244065E-4
90-94	0.2463348036126864	4.200798151648813E-4	0.0	0.0010501995379122034	0.028691451375761394	2.1003990758244065E-4
95-99	0.24637681159420294	4.200798151648813E-4	0.0	0.0010501995379122034	0.028985507246376812	2.1003990758244065E-4
100-104	0.24658685150178533	4.200798151648813E-4	0.0	0.0011342155009451797	0.02957361898760764	2.1003990758244065E-4
105-109	0.24658685150178533	4.200798151648813E-4	0.0	0.001260239445494644	0.030497794580970385	2.1003990758244065E-4
110-114	0.24658685150178533	4.200798151648813E-4	0.0	0.0014282713715605966	0.031127914303717707	2.1003990758244065E-4
115-119	0.24679689140936775	4.200798151648813E-4	0.0	0.0014702793530770846	0.03268220961982777	2.1003990758244065E-4
120-124	0.24793110691031295	4.200798151648813E-4	4.200798151648813E-5	0.0014702793530770846	0.034950640621718126	2.1003990758244065E-4
125-129	0.24830917874396136	4.200798151648813E-4	2.1003990758244065E-4	0.0015122873345935726	0.03889939088426801	2.1003990758244065E-4
130-134	0.24906532241125817	4.200798151648813E-4	2.1003990758244065E-4	0.0016803192606595252	0.042722117202268435	2.1003990758244065E-4
135-139	0.2499474900231044	4.200798151648813E-4	2.1003990758244065E-4	0.001890359168241966	0.046670867464818314	2.1003990758244065E-4
140-144	0.25015752993068685	4.200798151648813E-4	2.1003990758244065E-4	0.001890359168241966	0.050115521949170336	2.1003990758244065E-4
145-149	0.2504935937828187	4.200798151648813E-4	2.1003990758244065E-4	0.001890359168241966	0.054274312119302656	2.520478890989288E-4
150-154	0.2521319050619618	4.200798151648813E-4	2.1003990758244065E-4	0.001890359168241966	0.05860113421550095	4.200798151648813E-4
155-159	0.2536862003780718	4.200798151648813E-4	2.1003990758244065E-4	0.0020583910943079184	0.06124763705103971	4.200798151648813E-4
160-164	0.25629069523209413	4.200798151648813E-4	2.1003990758244065E-4	0.002100399075824407	0.06351606805293006	4.200798151648813E-4
165-167	0.25967933907442414	4.2007981516488136E-4	2.1003990758244068E-4	0.002100399075824407	0.06637261079605124	4.2007981516488136E-4
>>END_MODULE
