##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293578
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6134714
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.75430248256072	39.0	37.0	39.0	35.0	39.0
2	39.418215910309755	40.0	39.0	41.0	36.0	41.0
3	39.498709964311296	40.0	39.0	41.0	37.0	41.0
4	39.5318891801639	40.0	39.0	41.0	37.0	41.0
5	39.551116645372545	40.0	39.0	41.0	37.0	41.0
6	39.53933060286103	40.0	39.0	41.0	37.0	41.0
7	39.56730892426281	40.0	39.0	41.0	37.0	41.0
8	39.5503695852814	40.0	39.0	41.0	37.0	41.0
9	39.5402344754784	40.0	39.0	41.0	37.0	41.0
10-14	39.4743219977329	40.0	39.0	41.0	36.8	41.0
15-19	39.31661319500795	40.0	39.0	41.0	36.0	41.0
20-24	39.11509032042896	40.0	38.0	41.0	35.6	41.0
25-29	38.845699017101694	40.0	38.0	41.0	35.0	41.0
30-34	38.643538264375486	40.0	37.6	41.0	34.8	41.0
35-39	38.145359082754304	39.4	36.4	40.4	34.0	41.0
40-44	37.771243321204544	38.8	35.2	40.0	33.8	41.0
45-49	37.10586667935946	37.4	35.0	40.0	33.0	41.0
50-54	36.42923366924685	36.2	35.0	39.2	33.0	40.8
55-59	35.810290194457316	35.0	34.2	38.0	33.0	40.0
60-64	35.18395152569459	35.0	33.8	36.6	32.2	39.2
65-69	34.91222488937545	35.0	34.0	36.0	32.0	38.6
70-74	34.7596131457799	35.0	34.0	35.4	32.0	37.4
75-79	34.6428485174696	35.0	34.0	35.0	32.0	37.0
80-84	34.610045553875864	35.0	34.0	35.0	32.0	37.6
85-89	34.48029828285394	34.8	33.8	35.0	31.6	38.0
90-94	38.2055060678356	39.8	37.6	40.6	32.8	40.6
95-99	38.47221494382437	40.0	38.0	41.0	33.0	41.0
100-104	38.34619081793143	40.0	37.0	41.0	33.0	41.0
105-109	38.175117283951536	40.0	36.8	41.0	33.0	41.0
110-114	37.95481446908197	40.0	36.0	41.0	32.6	41.0
115-119	37.68098614931682	39.2	35.2	40.4	32.0	41.0
120-124	37.35776311949203	38.8	35.0	40.0	32.0	41.0
125-129	36.85368530506831	38.0	34.6	39.8	31.6	40.6
130-134	36.583618055801516	37.2	34.0	40.0	31.0	41.0
135-139	36.239640510662596	36.2	34.0	40.0	31.4	41.0
140-144	35.923202020013015	35.4	34.0	39.0	31.4	41.0
145-149	35.38372588494429	35.0	34.0	37.4	31.0	39.8
150-154	34.8711355428983	35.0	34.0	36.2	31.0	39.0
155-159	34.468530157569845	35.0	34.0	35.4	31.0	37.0
160-164	34.1727109458332	35.0	34.0	35.0	31.0	36.0
165-169	33.988727604347275	35.0	33.2	35.0	31.0	36.0
170-174	33.894298790311204	35.0	33.0	35.0	31.0	35.2
175-178	33.64071880060419	34.75	32.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	3.0
28	101.0
29	1437.0
30	8666.0
31	36195.0
32	117229.0
33	252406.0
34	443684.0
35	801253.0
36	1392825.0
37	1734120.0
38	1142050.0
39	203425.0
40	1320.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.592707663307532	27.126138235621088	27.371724256420105	22.909429844651275
2	23.255868162721196	27.164379627151327	27.5373391489807	22.042413061146778
3	21.41923812585232	26.233366380242014	28.803836658074033	23.54355883583163
4	22.458047759031636	25.581779362493506	28.843153894378776	23.11701898409608
5	23.09705065305408	26.248917227437175	27.427635583337707	23.22639653617104
6	23.265208451445332	26.322889706023783	27.068417533400908	23.343484309129977
7	23.356801963384115	26.599887134102744	26.73792127880778	23.30538962370536
8	23.26149189676976	26.82837374325845	26.7560476331904	23.154086726781394
9	23.511087884455577	26.74069239413606	26.57139354825669	23.176826173151674
10-14	23.360847791763398	26.76463809070806	26.617354941077938	23.257159176450607
15-19	23.34622608323713	26.72828757787242	26.678883481772747	23.246602857117708
20-24	23.224776248737918	26.665833158644396	26.816366663547804	23.293023929069882
25-29	23.237304950157416	26.708162760317762	26.66677207772033	23.387760211804494
30-34	23.270620276674673	26.720094856907757	26.653679372828137	23.355605493589433
35-39	23.240591166923185	26.73393413287074	26.643696837374982	23.381777862831097
40-44	23.323620302429745	26.730400145793265	26.601298772852328	23.344680778924655
45-49	23.5436305588166	26.60610095270945	26.47189420729312	23.378374281180832
50-54	23.46323887307542	26.68493429359543	26.536327528879095	23.315499304450054
55-59	23.388927340378054	26.688337875245693	26.521132036473094	23.401602747903162
60-64	23.395551936080476	26.71208144340551	26.516166849831958	23.376199770682057
65-69	23.337185726995585	26.70847247320739	26.569998210185513	23.38434358961151
70-74	23.26810019179378	26.788994564375777	26.550183105520485	23.392722138309953
75-79	23.31146651661349	26.744435029897073	26.53629492752229	23.407803525967143
80-84	23.249501117737516	26.790526828145534	26.53848247856379	23.421489575553156
85-89	23.244314241870118	26.8037401580579	26.48273741856589	23.469208181506097
90-94	22.334112243572655	26.727558699261433	27.811554256335334	23.126774800830578
95-99	22.908389409522922	26.86139292169362	26.876365391836636	23.353852276946814
100-104	22.989558787761883	26.90291428106652	26.737555448655325	23.36997148251627
105-109	22.913623617583912	26.91779161475376	26.797921528947093	23.37066323871523
110-114	22.92687557658106	26.790594288926066	26.837763726169623	23.444766408323254
115-119	22.902979811324048	26.861322371373024	26.75915677756111	23.476541039741814
120-124	22.88503122973806	26.886254671532484	26.751862267798593	23.476851830930865
125-129	22.911670460856016	26.86312179241267	26.763892496647163	23.46131525008415
130-134	23.052343373532764	26.84558862301386	26.630060872563003	23.47200713089037
135-139	23.212557835372824	26.844727757796022	26.525450457619222	23.417263949211932
140-144	23.16996054038323	26.887165860326785	26.643181875171447	23.29969172411854
145-149	23.086346693536957	26.959037667781523	26.680627520987223	23.2739881176943
150-154	22.98368611082672	27.05186986613301	26.7445081986367	23.21993582440357
155-159	22.99418810012859	27.061503105917083	26.79062561797251	23.15368317598182
160-164	22.866074017007406	27.224372270516582	26.860717671253344	23.04883604122266
165-169	22.761065749680114	27.305156259717627	26.926828115546552	23.006949875055703
170-174	22.715723570690393	27.410526409013876	26.975312169403654	22.898437850892073
175-178	22.611104211937345	27.533643120747552	27.00984168902505	22.845410978290055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	1.0
13	5.5
14	11.0
15	32.0
16	79.0
17	170.0
18	379.5
19	792.0
20	1592.0
21	2877.0
22	4972.5
23	8111.5
24	12378.5
25	17897.5
26	24670.5
27	32862.5
28	42321.0
29	52878.5
30	64461.5
31	76676.5
32	89633.0
33	103544.0
34	118021.5
35	132790.5
36	147747.0
37	163181.0
38	178089.0
39	192042.5
40	205218.0
41	216379.5
42	225383.5
43	232156.5
44	236219.5
45	237383.0
46	235929.5
47	231267.0
48	224282.5
49	216565.5
50	207445.0
51	197671.0
52	187560.0
53	177286.5
54	167685.5
55	158775.0
56	150143.5
57	141680.5
58	133417.5
59	125184.5
60	116344.5
61	106418.0
62	95889.0
63	85108.0
64	74418.0
65	63605.5
66	52978.0
67	43078.0
68	34032.5
69	26195.0
70	19607.0
71	14338.5
72	10129.5
73	6834.5
74	4514.0
75	2827.0
76	1697.5
77	1023.0
78	591.5
79	305.0
80	156.5
81	79.5
82	36.0
83	20.0
84	9.5
85	3.5
86	1.5
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2286.0
92-93	4709.0
94-95	6524.0
96-97	8722.0
98-99	10983.0
100-101	12803.0
102-103	14180.0
104-105	16042.0
106-107	17246.0
108-109	18539.0
110-111	20276.0
112-113	21235.0
114-115	22094.0
116-117	23049.0
118-119	24296.0
120-121	24981.0
122-123	26438.0
124-125	26831.0
126-127	28160.0
128-129	28721.0
130-131	29972.0
132-133	31722.0
134-135	132041.0
136-137	110412.0
138-139	99730.0
140-141	109354.0
142-143	109485.0
144-145	98805.0
146-147	97089.0
148-149	99396.0
150-151	99956.0
152-153	99698.0
154-155	102123.0
156-157	88212.0
158-159	94337.0
160-161	93877.0
162-163	94571.0
164-165	92602.0
166-167	96215.0
168-169	95296.0
170-171	113908.0
172-173	92570.0
174-175	104219.0
176-177	202909.0
178-179	3388100.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.20927217186633
#Duplication Level	Percentage of total
1	94.60920205840603
2	4.889555931806518
3	0.39015162434378264
4	0.07170409829037769
5	0.02478512422549773
6	0.011452403472726446
7	0.003148759455056702
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.630067840163372E-5	0.0	0.0	0.0	0.0	0.0
2	3.260135680326744E-5	1.630067840163372E-5	0.0	0.0	0.0	0.0
3	3.260135680326744E-5	1.630067840163372E-5	0.0	0.0	0.0	0.0
4	3.260135680326744E-5	1.630067840163372E-5	0.0	0.0	1.630067840163372E-5	0.0
5	4.8902035204901156E-5	1.630067840163372E-5	0.0	0.0	1.630067840163372E-5	0.0
6	6.520271360653488E-5	1.630067840163372E-5	0.0	0.0	1.630067840163372E-5	0.0
7	6.520271360653488E-5	3.260135680326744E-5	0.0	0.0	1.630067840163372E-5	0.0
8	6.520271360653488E-5	3.260135680326744E-5	0.0	0.0	1.630067840163372E-5	0.0
9	6.520271360653488E-5	3.260135680326744E-5	1.630067840163372E-5	1.630067840163372E-5	1.630067840163372E-5	0.0
10-14	6.520271360653488E-5	4.564189952457441E-5	2.6081085442613952E-5	5.542230656555465E-5	1.630067840163372E-5	0.0
15-19	1.108446131311093E-4	6.520271360653488E-5	5.8682442245881393E-5	6.520271360653488E-5	1.630067840163372E-5	0.0
20-24	1.3366556289339651E-4	8.15033920081686E-5	6.520271360653488E-5	8.476352768849533E-5	1.630067840163372E-5	0.0
25-29	1.499662412950302E-4	1.3692569857372324E-4	7.824325632784185E-5	1.0758447745078256E-4	3.586149248359418E-5	0.0
30-34	2.3472976898352557E-4	1.499662412950302E-4	8.15033920081686E-5	1.3040542721306977E-4	5.2162170885227904E-5	0.0
35-39	5.868244224588139E-4	1.630067840163372E-4	9.780407040980231E-5	1.7278719105731744E-4	8.80236633688221E-5	0.0
40-44	0.0011051859956307663	1.760473267376442E-4	1.0432434177045581E-4	1.793074624179709E-4	1.1410474881143604E-4	0.0
45-49	0.001512702955671609	2.0212841218025815E-4	1.3040542721306977E-4	1.9560814081960465E-4	1.5322637697535698E-4	2.9341221122940697E-5
50-54	0.0017702536744174217	2.3798990466385232E-4	1.3040542721306977E-4	2.4777031170483254E-4	1.630067840163372E-4	3.9121628163920924E-5
55-59	0.0020278043931632344	2.7711153282777325E-4	1.5322637697535698E-4	3.1623316099169417E-4	1.7278719105731744E-4	5.542230656555465E-5
60-64	0.0022266726696631664	2.7711153282777325E-4	1.8908786945895113E-4	3.7817573891790225E-4	2.2494936194254533E-4	6.520271360653488E-5
65-69	0.00240271999640081	2.9341221122940696E-4	2.1842909058189183E-4	4.433784525244372E-4	2.5755071874581276E-4	6.520271360653488E-5
70-74	0.0038763013239084985	3.520946534752883E-4	2.2820949762287208E-4	4.6619940228672437E-4	2.999324825900604E-4	8.15033920081686E-5
75-79	0.03231446486339869	3.7491560323757553E-4	2.4451017602450576E-4	4.890203520490115E-4	3.260135680326744E-4	8.15033920081686E-5
80-84	0.04493118994626318	4.2381763844247667E-4	2.510304473851592E-4	4.890203520490115E-4	3.81435874598229E-4	8.15033920081686E-5
85-89	0.04508767645891886	4.955406234096651E-4	2.6733112578679303E-4	5.248818445326058E-4	5.574832013358732E-4	8.15033920081686E-5
90-94	0.04525720351429585	5.314021158932592E-4	2.7711153282777325E-4	5.770440154178336E-4	5.705237440571802E-4	8.15033920081686E-5
95-99	0.04540064948423024	5.379223872539128E-4	2.9015207554908024E-4	6.324663219833883E-4	5.705237440571802E-4	8.802366336882208E-5
100-104	0.045524534640082645	5.640034726965268E-4	3.031926182703872E-4	6.911487642292696E-4	5.705237440571802E-4	9.780407040980231E-5
105-109	0.04572666305226291	6.194257792620813E-4	3.325338393933279E-4	7.726521562374381E-4	5.868244224588139E-4	9.780407040980231E-5
110-114	0.045932051600123494	6.194257792620813E-4	3.7491560323757553E-4	8.019933773603788E-4	5.868244224588139E-4	9.780407040980231E-5
115-119	0.046124399605262774	6.194257792620813E-4	3.7491560323757553E-4	8.378548698439731E-4	5.868244224588139E-4	9.780407040980231E-5
120-124	0.04643085235921348	6.194257792620813E-4	3.81435874598229E-4	8.997974477701813E-4	5.966048294997941E-4	9.780407040980231E-5
125-129	0.04714482207320504	6.292061863030616E-4	4.009966886801894E-4	9.68260297057043E-4	6.422467290243686E-4	9.780407040980231E-5
130-134	0.04780336948063104	6.618075431063289E-4	4.3685818116378367E-4	9.976015181799834E-4	6.74848085827636E-4	9.780407040980231E-5
135-139	0.04851081892326195	6.715879501473092E-4	4.4011831684411044E-4	0.0010595440961061917	7.009291712702499E-4	9.780407040980231E-5
140-144	0.04917914673772893	7.0092917127025E-4	4.4011831684411044E-4	0.0010986657242701126	7.237501210325371E-4	1.1410474881143604E-4
145-149	0.050897238241261133	7.172298496718836E-4	4.498987238850907E-4	0.0011247468097127268	7.628717491964582E-4	1.1410474881143604E-4
150-154	0.05232517766924424	7.302703923931905E-4	4.825000806883581E-4	0.0011638684378766475	7.824325632784185E-4	1.1410474881143604E-4
155-159	0.052889181141940765	7.628717491964582E-4	4.890203520490115E-4	0.0012323312871635093	7.889528346390719E-4	1.1410474881143604E-4
160-164	0.053401022443752055	7.661318848767848E-4	4.890203520490115E-4	0.00126167250828645	8.085136487210324E-4	1.1410474881143604E-4
165-167	0.053759637368588	7.661318848767848E-4	4.890203520490115E-4	0.0012714529153274301	8.150339200816859E-4	1.1410474881143604E-4
>>END_MODULE
