##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293581
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5982791
Total Bases	992.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.59750892183932	39.0	37.0	39.0	35.0	39.0
2	39.24009061991301	40.0	39.0	41.0	36.0	41.0
3	39.3376755096409	40.0	39.0	41.0	36.0	41.0
4	39.388381275561855	40.0	39.0	41.0	36.0	41.0
5	39.402359701350086	40.0	39.0	41.0	36.0	41.0
6	39.39216596401245	40.0	39.0	41.0	36.0	41.0
7	39.431113338239626	40.0	39.0	41.0	37.0	41.0
8	39.41208542969326	40.0	39.0	41.0	37.0	41.0
9	39.40998423645419	40.0	39.0	41.0	37.0	41.0
10-14	39.34326751511126	40.0	39.0	41.0	36.0	41.0
15-19	39.193300217239745	40.0	38.4	41.0	36.0	41.0
20-24	39.0158194394556	40.0	38.0	41.0	35.4	41.0
25-29	38.80339921618522	40.0	38.0	41.0	35.0	41.0
30-34	38.652622831049925	40.0	37.8	41.0	34.8	41.0
35-39	38.22233766815522	39.8	36.6	40.0	34.0	41.0
40-44	37.8923104952187	39.0	35.6	40.0	33.8	41.0
45-49	37.197214711327874	37.8	35.0	40.0	33.0	41.0
50-54	36.42134381762626	36.4	34.8	39.2	33.0	40.6
55-59	35.68215139054665	35.2	34.0	37.6	32.4	39.8
60-64	34.97772300586799	35.0	33.8	36.4	31.8	38.8
65-69	34.6643922878135	35.0	33.8	36.0	32.0	37.4
70-74	34.5179493316748	35.0	33.8	35.0	32.0	37.0
75-79	34.43332417930027	35.0	33.0	35.0	32.0	37.0
80-84	34.40665361701587	35.0	33.0	35.0	32.0	37.0
85-89	34.28008991121368	34.8	33.0	35.0	31.6	37.2
90-94	37.44726475124229	39.6	35.6	40.2	31.4	40.2
95-99	38.01399235366241	40.0	36.0	41.0	32.0	41.0
100-104	37.909635415848854	40.0	36.0	41.0	32.0	41.0
105-109	37.76601836184733	40.0	36.0	41.0	32.0	41.0
110-114	37.57136371063523	39.6	35.0	40.4	31.2	41.0
115-119	37.35048418137096	39.0	35.0	40.0	31.0	41.0
120-124	37.07747797277243	38.6	34.6	40.0	31.0	41.0
125-129	36.7332921760796	37.8	34.0	39.8	30.8	40.8
130-134	36.369504835572066	37.0	34.0	40.0	30.0	40.8
135-139	36.06756356255359	36.2	34.0	40.0	30.2	41.0
140-144	35.78841831749974	35.6	34.0	39.0	31.0	40.8
145-149	35.222110293375195	35.0	33.6	37.6	30.4	39.8
150-154	34.67808857291951	35.0	33.0	36.2	30.0	38.8
155-159	34.24709829072806	35.0	33.0	35.4	30.0	37.0
160-164	33.95669596839832	35.0	33.0	35.0	30.0	36.0
165-169	33.78469294809867	35.0	33.0	35.0	30.0	36.0
170-174	33.71120519738672	35.0	33.0	35.0	30.0	35.0
175-179	33.52877606379016	34.8	32.8	35.0	29.8	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	2.0
28	145.0
29	2199.0
30	12943.0
31	52546.0
32	168752.0
33	348369.0
34	516382.0
35	789218.0
36	1271276.0
37	1659635.0
38	1038737.0
39	122224.0
40	363.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.28078834777949	27.547577710804205	27.041459412504963	23.13017452891134
2	22.2152336593406	27.48961145391841	28.33242879452082	21.96272609222017
3	21.108241955970048	27.076092078095325	28.85604728629163	22.959618679642997
4	21.93447840648286	26.723865165940108	28.84479835581754	22.496858071759483
5	22.228103906688368	27.303176728052176	28.02673534810091	22.441984017158546
6	22.6368261903182	27.13673267209234	27.51022056428179	22.716220573307673
7	22.45786289375644	27.601616035057887	27.4867198269169	22.45380124426877
8	22.255733151968705	27.754738549282433	27.7093416768194	22.280186621929467
9	22.804289837301685	27.5236256790518	27.07455433425637	22.597530149390145
10-14	22.534372335587186	27.637853302914976	27.311684462987255	22.51608989851058
15-19	22.561968151653637	27.549399602961227	27.342255479089943	22.546376766295197
20-24	22.41973018947177	27.569062666571504	27.443265191780892	22.567941952175833
25-29	22.40128060632571	27.66381777334358	27.359421380422617	22.575480239908096
30-34	22.479070387048452	27.672930577050074	27.291215755322224	22.55678328057925
35-39	22.4162769516769	27.628767242579595	27.35560710711773	22.599348698625775
40-44	22.464916457887295	27.721753943936868	27.305653832801447	22.507675765374387
45-49	22.738818721897523	27.527704043146418	27.149058023253698	22.584419211702365
50-54	22.628117880099772	27.578539848709404	27.245484590720288	22.547857680470536
55-59	22.564595687865413	27.610521577638263	27.258411667731664	22.56647106676466
60-64	22.6054361584752	27.600827105610076	27.20131791332841	22.592418822586318
65-69	22.494996064545795	27.600335696165885	27.299797034527867	22.604871204760453
70-74	22.430474338816115	27.682394387502423	27.30668345258927	22.580447821092196
75-79	22.49463837195717	27.591985078536084	27.27114485530248	22.64223169420426
80-84	22.414558690082938	27.633427274995903	27.314037879645138	22.637976155276025
85-89	22.399338368998684	27.675260593258226	27.252966048788934	22.67243498895415
90-94	21.655330625811125	27.182870386008197	28.39548976090941	22.766309227271268
95-99	22.055904105770484	27.588351179711502	27.69742582037717	22.65831889414084
100-104	22.122999881503393	27.713110119041108	27.51635658932218	22.64753341013332
105-109	22.206016578856485	27.619448280731127	27.516910283198925	22.65762485721346
110-114	22.128431460807953	27.578269852680343	27.60899480485439	22.684303881657318
115-119	22.10176608336339	27.611849466590364	27.555324769720063	22.731059680326183
120-124	22.185263638752197	27.628140543745022	27.49373297489924	22.69286284260355
125-129	22.13639471627557	27.61101713531045	27.561664306935835	22.69092384147814
130-134	22.2568305592692	27.65877364715314	27.461869708130994	22.62252608544667
135-139	22.504171975017535	27.54613846631701	27.319121857204188	22.630567701461267
140-144	22.378281457787352	27.64625487780718	27.46573838502609	22.509725279379374
145-149	22.316465298774606	27.699660402533432	27.49741953914907	22.486454759542884
150-154	22.35816385473295	27.740595199120342	27.46595018809038	22.43529075805633
155-159	22.230389215042372	27.761371848572512	27.588054462000656	22.42018447438446
160-164	22.1265765889035	27.887622125777412	27.65971132266973	22.326089962649366
165-169	22.159980202785874	27.874494946888444	27.66021855298654	22.305306297339143
170-174	22.064249930375418	27.985313377800214	27.72240309047394	22.228033601350425
175-179	21.982248501562406	28.100694865077514	27.76876254262854	22.14829409073154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	2.5
13	4.0
14	10.0
15	25.0
16	52.0
17	105.5
18	242.0
19	526.5
20	1127.5
21	2201.5
22	3935.0
23	6639.5
24	10623.5
25	15952.0
26	22854.0
27	31171.5
28	40905.0
29	52538.5
30	65541.5
31	79245.0
32	93694.0
33	109232.0
34	125939.5
35	143004.5
36	160572.0
37	178480.5
38	195798.5
39	212808.0
40	228169.5
41	242083.0
42	253940.0
43	262147.5
44	267971.0
45	269882.5
46	267518.5
47	262495.5
48	253326.5
49	240701.5
50	225758.5
51	208909.0
52	190865.5
53	171209.5
54	151594.0
55	133562.5
56	116858.5
57	101724.5
58	88388.0
59	76555.5
60	66446.5
61	57818.0
62	50314.5
63	43756.5
64	37992.0
65	32859.5
66	27947.0
67	23339.0
68	19236.5
69	15475.5
70	12069.5
71	9161.0
72	6711.5
73	4775.5
74	3384.0
75	2310.5
76	1490.0
77	938.0
78	562.0
79	317.5
80	187.5
81	102.0
82	47.0
83	24.0
84	10.0
85	3.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1912.0
92-93	4316.0
94-95	6310.0
96-97	8437.0
98-99	10196.0
100-101	11818.0
102-103	13285.0
104-105	14562.0
106-107	16271.0
108-109	17959.0
110-111	18993.0
112-113	20153.0
114-115	20569.0
116-117	21614.0
118-119	23118.0
120-121	23801.0
122-123	24387.0
124-125	25015.0
126-127	26193.0
128-129	27383.0
130-131	27970.0
132-133	29719.0
134-135	116411.0
136-137	118090.0
138-139	103203.0
140-141	104773.0
142-143	114959.0
144-145	101920.0
146-147	105954.0
148-149	112367.0
150-151	104443.0
152-153	91951.0
154-155	97210.0
156-157	94846.0
158-159	90118.0
160-161	101189.0
162-163	104593.0
164-165	84858.0
166-167	91806.0
168-169	95255.0
170-171	93712.0
172-173	91681.0
174-175	97398.0
176-177	107214.0
178-179	3364859.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.09076912462763
#Duplication Level	Percentage of total
1	85.64441015046125
2	10.822738667263486
3	2.28637787465904
4	0.7228111844995132
5	0.29733279785624045
6	0.10722778847480993
7	0.05773540778361519
8	0.027520223153879187
9	0.010672527727379104
>10	0.02317337812081291
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.671460694515319E-5	1.671460694515319E-5	0.0	0.0	1.671460694515319E-5	0.0
2	5.682966361352085E-4	3.342921389030638E-5	1.671460694515319E-5	1.671460694515319E-5	1.671460694515319E-5	0.0
3	5.682966361352085E-4	6.685842778061276E-5	1.671460694515319E-5	1.671460694515319E-5	1.671460694515319E-5	0.0
4	5.682966361352085E-4	6.685842778061276E-5	3.342921389030638E-5	1.671460694515319E-5	1.671460694515319E-5	0.0
5	5.682966361352085E-4	6.685842778061276E-5	3.342921389030638E-5	3.342921389030638E-5	1.671460694515319E-5	0.0
6	5.682966361352085E-4	6.685842778061276E-5	5.014382083545957E-5	5.014382083545957E-5	1.671460694515319E-5	0.0
7	5.682966361352085E-4	6.685842778061276E-5	5.014382083545957E-5	5.014382083545957E-5	3.342921389030638E-5	0.0
8	5.682966361352085E-4	8.357303472576594E-5	6.685842778061276E-5	5.014382083545957E-5	3.342921389030638E-5	0.0
9	5.682966361352085E-4	8.357303472576594E-5	6.685842778061276E-5	5.014382083545957E-5	3.342921389030638E-5	0.0
10-14	5.749824789132698E-4	1.0028764167091914E-4	6.685842778061276E-5	8.357303472576594E-5	4.3457978057398296E-5	0.0
15-19	5.850112430803617E-4	1.0363056305994978E-4	6.685842778061276E-5	1.2368809139413358E-4	5.6829663613520846E-5	0.0
20-24	6.017258500255148E-4	1.2368809139413358E-4	8.691595611479659E-5	1.671460694515319E-4	7.354427055867403E-5	0.0
25-29	6.017258500255148E-4	1.6380314806250127E-4	1.0028764167091914E-4	1.671460694515319E-4	9.694472028188851E-5	0.0
30-34	7.48814391142863E-4	1.8051775500765445E-4	1.0028764167091914E-4	2.0057528334183828E-4	1.404026983392868E-4	0.0
35-39	0.0015344009175650628	2.0726112611989955E-4	1.1365932722704169E-4	2.941770822346961E-4	1.5711730528443997E-4	1.671460694515319E-5
40-44	0.0025071910417729785	2.4403326139923657E-4	1.2703101278316423E-4	3.309492175140332E-4	2.5071910417729786E-4	3.008629250127574E-5
45-49	0.0032125474548584435	2.6743371112245104E-4	1.504314625063787E-4	4.044934880727072E-4	3.1089168917984935E-4	5.348674222449021E-5
50-54	0.0038978463396097244	2.6743371112245104E-4	1.504314625063787E-4	4.847236014094425E-4	3.7775011696046214E-4	7.688719194770468E-5
55-59	0.0043825699410191665	2.6743371112245104E-4	1.504314625063787E-4	5.582678719681166E-4	4.312368591849523E-4	8.357303472576594E-5
60-64	0.0047970921932589655	2.8414831806760423E-4	1.5377438389540935E-4	6.017258500255148E-4	4.646660730752587E-4	8.357303472576594E-5
65-69	0.005021067926324018	2.8414831806760423E-4	1.671460694515319E-4	6.919847275293421E-4	5.081240511326569E-4	1.0363056305994978E-4
70-74	0.00780572144338654	2.8414831806760423E-4	1.671460694515319E-4	7.588431553099548E-4	5.983829286364842E-4	1.1700224861607231E-4
75-79	0.060396560735616535	2.9752000362372676E-4	1.671460694515319E-4	8.156728189234756E-4	6.552125922500051E-4	1.5377438389540935E-4
80-84	0.08292785089768304	3.2426337473597194E-4	2.0726112611989955E-4	8.524449542028127E-4	7.555002339209242E-4	1.671460694515319E-4
85-89	0.08307828236018941	3.342921389030638E-4	2.1728989028699147E-4	8.992458536492417E-4	9.393609103176094E-4	1.671460694515319E-4
90-94	0.08325545719380804	3.4766382445918637E-4	2.1728989028699147E-4	9.727901242079156E-4	9.661042814298543E-4	1.671460694515319E-4
95-99	0.08343263202742666	3.777501169604621E-4	2.1728989028699147E-4	9.961905739311301E-4	9.694472028188852E-4	1.671460694515319E-4
100-104	0.08379366753744197	4.178651736288297E-4	2.2731865445408338E-4	0.0010496773161556203	9.79475966985977E-4	1.671460694515319E-4
105-109	0.0841781034971805	4.3457978057398293E-4	2.3400449723214462E-4	0.0010998211369910801	9.861618097640383E-4	1.671460694515319E-4
110-114	0.08453579608580677	4.3457978057398293E-4	2.3400449723214462E-4	0.0011700224861607234	9.861618097640383E-4	1.671460694515319E-4
115-119	0.08494363249526851	4.412656233520443E-4	2.507191041772978E-4	0.0012335379925523054	0.0010229339450433753	1.671460694515319E-4
120-124	0.08543169901806699	4.746948372423506E-4	2.7411955390051234E-4	0.0012970534989438878	0.0010897923728239883	1.671460694515319E-4
125-129	0.08646466172727744	5.014382083545957E-4	3.008629250127574E-4	0.0014341132758941438	0.0011165357439362332	1.671460694515319E-4
130-134	0.08747088106537568	5.014382083545957E-4	3.209204533469412E-4	0.001514343389230879	0.0011666795647716928	1.838606763966851E-4
135-139	0.08881807838515501	5.014382083545957E-4	3.409779816811251E-4	0.0015477726031211854	0.0012435667567193974	2.0057528334183828E-4
140-144	0.0903758797524433	5.014382083545957E-4	3.51006745848217E-4	0.0016480602447921044	0.0012870247347767957	2.0057528334183828E-4
145-149	0.09200388246890122	5.081240511326569E-4	3.51006745848217E-4	0.0017583766506301158	0.0013237968700561327	2.0057528334183828E-4
150-154	0.09348813956563082	5.181528152997489E-4	3.51006745848217E-4	0.0018085204714655752	0.0013538831625574085	2.0057528334183828E-4
155-159	0.09444755800428262	5.348674222449021E-4	3.51006745848217E-4	0.0018720359778571574	0.0013605690053354698	2.0057528334183828E-4
160-164	0.09556743666960787	5.348674222449021E-4	3.51006745848217E-4	0.0019088081131364942	0.0014006840620038375	2.0057528334183828E-4
165-168	0.0963764236457533	5.515820291900553E-4	3.593640493207936E-4	0.001993216878209518	0.001404026983392868	2.0475393507812657E-4
>>END_MODULE
