##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293585
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5213787
Total Bases	857.9 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.45587477969468	39.0	37.0	39.0	35.0	39.0
2	39.07627872024691	40.0	38.0	41.0	36.0	41.0
3	39.18779881111369	40.0	38.0	41.0	36.0	41.0
4	39.24984353983007	40.0	39.0	41.0	36.0	41.0
5	39.27292158271905	40.0	39.0	41.0	36.0	41.0
6	39.26573084017433	40.0	39.0	41.0	36.0	41.0
7	39.309375891266754	40.0	39.0	41.0	36.0	41.0
8	39.286334673817706	40.0	39.0	41.0	36.0	41.0
9	39.27949396475153	40.0	39.0	41.0	36.0	41.0
10-14	39.20613461961526	40.0	38.8	41.0	36.0	41.0
15-19	39.05147958671883	40.0	38.0	41.0	36.0	41.0
20-24	38.8665374323884	40.0	38.0	41.0	35.0	41.0
25-29	38.65349996077707	40.0	38.0	40.2	35.0	41.0
30-34	38.51719907238251	40.0	37.4	40.0	34.4	41.0
35-39	38.088220481580855	39.6	36.4	40.0	33.8	41.0
40-44	37.7587947110229	39.0	35.6	40.0	33.4	41.0
45-49	37.09099466472259	37.6	35.0	40.0	33.0	41.0
50-54	36.370196672783145	36.2	34.6	39.2	33.0	40.4
55-59	35.68434456566791	35.2	34.0	37.8	32.0	40.0
60-64	35.00737548350173	35.0	33.8	36.6	31.8	39.0
65-69	34.7087216643104	35.0	33.8	36.0	31.8	38.4
70-74	34.56559767401315	35.0	33.4	35.2	32.0	37.0
75-79	34.47733123735204	35.0	33.0	35.0	32.0	37.0
80-84	34.44974180955226	35.0	33.0	35.0	32.0	37.8
85-89	34.327280918840756	34.8	32.8	35.0	31.0	38.2
90-94	37.75177793824217	39.8	36.2	40.6	31.6	40.6
95-99	38.07805624931593	40.0	36.8	41.0	32.0	41.0
100-104	37.95808841516739	40.0	36.0	41.0	32.0	41.0
105-109	37.79969730888091	40.0	36.0	41.0	32.0	41.0
110-114	37.594784421663675	39.4	35.0	40.2	32.0	41.0
115-119	37.362634799263944	39.0	35.0	40.0	31.2	41.0
120-124	37.0920321680395	38.4	34.8	40.0	31.0	41.0
125-129	36.665881441574	37.6	34.0	39.8	31.0	40.6
130-134	36.425664387115134	37.0	34.0	40.0	30.8	41.0
135-139	36.07930934395587	36.0	34.0	39.8	30.8	41.0
140-144	35.74838807888456	35.4	34.0	39.0	31.0	40.6
145-149	35.193298315500215	35.0	34.0	37.2	31.0	39.8
150-154	34.68439925352426	35.0	33.4	36.2	31.0	38.6
155-159	34.28234478755601	35.0	33.0	35.4	30.2	37.0
160-164	34.003802297912614	35.0	33.0	35.0	30.0	36.0
165-169	33.83789354689962	35.0	33.0	35.0	30.0	36.0
170-174	33.76700674705918	35.0	33.0	35.0	30.0	35.0
175-178	33.53442835019811	34.75	32.75	35.0	29.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	120.0
29	1937.0
30	11053.0
31	44783.0
32	137853.0
33	283167.0
34	448253.0
35	721591.0
36	1154835.0
37	1430856.0
38	860777.0
39	118184.0
40	377.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.609187333506334	27.775799049711853	28.48647250069863	22.128541116083184
2	21.95206670314687	28.26745703267126	28.911710432359435	20.868765831822436
3	20.167317920735925	26.75302999527982	30.322719359268035	22.75693272471622
4	21.067680747218866	26.00881087010267	30.583566225471042	22.33994215720742
5	22.070425968686486	26.844345578367506	28.63922135676045	22.446007096185554
6	22.446831832600754	26.86878079215741	27.99304996540902	22.691337409832816
7	22.371646559401064	27.44490712796668	27.63624597629324	22.547200336339017
8	22.344353538032912	27.674797608724717	27.61700851991077	22.36384033333161
9	22.77152864127361	27.417748366014955	27.13016469602613	22.68055829668531
10-14	22.52803192765642	27.709152675396982	27.229194441583438	22.53362095536316
15-19	22.49883625855832	27.59452198565074	27.36077634164955	22.545865414141392
20-24	22.318015676513063	27.515761575990734	27.597663655995152	22.568559091501054
25-29	22.34220538737006	27.606685888779115	27.396374266919608	22.65473445693121
30-34	22.413374386026895	27.61053721603894	27.335105941228516	22.640982456705654
35-39	22.39460108362693	27.574018654770516	27.360596050433205	22.670784211169348
40-44	22.450057894578354	27.60781750386044	27.328891648239562	22.613232953321646
45-49	22.736398705969386	27.436368229081854	27.150829138206067	22.67640392674269
50-54	22.644822276015493	27.483474104331457	27.257066696433895	22.61463692321915
55-59	22.57281319701016	27.515166998575125	27.241600011661387	22.67041979275333
60-64	22.589526576363784	27.499961160668818	27.22418848334234	22.68632377962506
65-69	22.52122305725186	27.470823031320613	27.31263091491847	22.695322996509063
70-74	22.443252860157116	27.599052281959352	27.26754276689861	22.69015209098492
75-79	22.494187046766584	27.550013071113185	27.224986367874255	22.73081351424598
80-84	22.435738168820478	27.583520385470294	27.239098950532505	22.74164249517673
85-89	22.41768219530257	27.624450327564205	27.189607093653805	22.768260383479415
90-94	21.32746733303153	27.33758085668596	28.941831081849678	22.39312072843283
95-99	22.115502282855502	27.614855085123935	27.59562155995819	22.674021072062377
100-104	22.2655124195425	27.720344139278204	27.3569346641859	22.6572087769934
105-109	22.150444335486828	27.686071205058717	27.531064929177763	22.632419530276696
110-114	22.174507140834844	27.532359709967558	27.565040274012325	22.72809287518527
115-119	22.202825055478346	27.589223164304187	27.440802102274585	22.767149677942882
120-124	22.17669008462789	27.64120477586586	27.447611587482207	22.734493552024045
125-129	22.252613110724116	27.603199709504462	27.43815429357019	22.706032886201235
130-134	22.366125287327385	27.59517984245916	27.343769281254378	22.69492558895908
135-139	22.489087593246623	27.59559067691088	27.260830077680403	22.65449165216209
140-144	22.49886755597488	27.600849458481747	27.329912875392363	22.570370110151007
145-149	22.425012373911798	27.66497449798433	27.381411660210947	22.528601467892926
150-154	22.339632224248017	27.76117942892827	27.446564349605424	22.45262399721829
155-159	22.339675133235666	27.741080738725206	27.46877950448013	22.450464623559004
160-164	22.23898684614954	27.916332463765325	27.513968205839802	22.33071248424533
165-169	22.135839399353564	27.98386533622677	27.581394318726836	22.298900945692825
170-174	22.174608963841873	28.08898442547752	27.580576374513072	22.15583023616753
175-178	22.076026780486252	28.210071755334077	27.595727963276882	22.11817350090279
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	2.5
13	4.5
14	12.0
15	23.5
16	41.5
17	103.0
18	257.0
19	560.5
20	1174.5
21	2301.0
22	4231.0
23	7271.5
24	11704.5
25	17758.0
26	25271.5
27	34250.5
28	44636.5
29	55587.0
30	67010.0
31	78732.5
32	91060.0
33	103690.5
34	116223.0
35	129329.5
36	142215.5
37	155459.5
38	168749.0
39	181668.5
40	193529.0
41	203224.5
42	211503.5
43	217364.5
44	220318.0
45	220621.5
46	217826.0
47	212067.5
48	203580.5
49	192908.5
50	181187.0
51	168385.5
52	154988.0
53	141826.5
54	129269.0
55	117420.5
56	105740.0
57	95057.5
58	85687.0
59	76799.0
60	68499.5
61	60914.5
62	53411.5
63	46387.5
64	40181.0
65	34209.5
66	28546.5
67	23357.5
68	18638.0
69	14539.5
70	11136.5
71	8447.0
72	6131.5
73	4253.0
74	2914.5
75	1906.5
76	1226.5
77	767.5
78	444.5
79	234.0
80	144.5
81	87.5
82	41.5
83	20.0
84	9.0
85	4.0
86	2.5
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1884.0
92-93	4204.0
94-95	6154.0
96-97	8223.0
98-99	9905.0
100-101	11685.0
102-103	13270.0
104-105	14509.0
106-107	16472.0
108-109	17467.0
110-111	18584.0
112-113	19771.0
114-115	20607.0
116-117	21485.0
118-119	22648.0
120-121	23268.0
122-123	23956.0
124-125	24701.0
126-127	26070.0
128-129	26764.0
130-131	27511.0
132-133	29707.0
134-135	109135.0
136-137	103752.0
138-139	90676.0
140-141	95448.0
142-143	100889.0
144-145	92418.0
146-147	95293.0
148-149	101343.0
150-151	93052.0
152-153	82182.0
154-155	85959.0
156-157	85019.0
158-159	81380.0
160-161	104687.0
162-163	78856.0
164-165	77160.0
166-167	83137.0
168-169	84834.0
170-171	83841.0
172-173	83177.0
174-175	86814.0
176-177	178609.0
178-179	2747281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.44139838427608
#Duplication Level	Percentage of total
1	88.17962134313937
2	9.075075301433904
3	1.505228552505593
4	0.4603815623187163
5	0.22308486989662005
6	0.1299065969006852
7	0.07086769486804413
8	0.06407297063195327
9	0.042045976988068785
>10	0.24788483415551146
>50	0.0018302971615493338
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.9179916632574366E-5	1.9179916632574366E-5	1.9179916632574366E-5	0.0
2	2.10979082958318E-4	0.0	1.9179916632574366E-5	1.9179916632574366E-5	1.9179916632574366E-5	0.0
3	2.3015899959089237E-4	5.753974989772309E-5	1.9179916632574366E-5	1.9179916632574366E-5	1.9179916632574366E-5	0.0
4	2.3015899959089237E-4	5.753974989772309E-5	1.9179916632574366E-5	1.9179916632574366E-5	1.9179916632574366E-5	0.0
5	2.3015899959089237E-4	5.753974989772309E-5	1.9179916632574366E-5	5.753974989772309E-5	1.9179916632574366E-5	0.0
6	2.3015899959089237E-4	5.753974989772309E-5	1.9179916632574366E-5	5.753974989772309E-5	1.9179916632574366E-5	0.0
7	2.3015899959089237E-4	5.753974989772309E-5	3.835983326514873E-5	5.753974989772309E-5	1.9179916632574366E-5	0.0
8	2.4933891622346677E-4	5.753974989772309E-5	3.835983326514873E-5	5.753974989772309E-5	1.9179916632574366E-5	0.0
9	2.4933891622346677E-4	5.753974989772309E-5	3.835983326514873E-5	7.671966653029746E-5	1.9179916632574366E-5	0.0
10-14	2.7619079950907087E-4	5.753974989772309E-5	5.753974989772309E-5	1.0357154981590155E-4	2.685188328560411E-5	0.0
15-19	2.9153473281513033E-4	5.753974989772309E-5	8.439163318332721E-5	1.304234331015057E-4	3.835983326514873E-5	0.0
20-24	3.452384993863386E-4	6.137573322423797E-5	1.1124351646893131E-4	1.4576736640756517E-4	3.835983326514873E-5	0.0
25-29	3.6441841601891293E-4	7.671966653029746E-5	1.1507949979544619E-4	1.7645523301968417E-4	4.986778324469334E-5	0.0
30-34	5.907414322832904E-4	9.589958316287182E-5	1.2658744977499082E-4	2.263230162643775E-4	6.904769987726772E-5	0.0
35-39	0.0017108485636256332	1.1507949979544619E-4	1.4960334973408007E-4	2.8386276616210063E-4	1.3425941642802056E-4	2.685188328560411E-5
40-44	0.0029460351947634225	1.1507949979544619E-4	1.572753163871098E-4	3.0687866612118985E-4	2.3015899959089237E-4	6.904769987726772E-5
45-49	0.003847491276494418	1.304234331015057E-4	1.7645523301968417E-4	3.260585827537642E-4	3.6058243269239805E-4	9.206359983635695E-5
50-54	0.0046185239251239065	1.4960334973408007E-4	1.9947113297877337E-4	3.452384993863386E-4	4.027782492840617E-4	9.589958316287182E-5
55-59	0.005316672890549614	1.726192496931693E-4	2.608468662030114E-4	3.5291046603936835E-4	4.0661423261057655E-4	1.1507949979544619E-4
60-64	0.0058882344062003295	1.726192496931693E-4	2.685188328560411E-4	3.797623493249724E-4	4.7182594916132944E-4	1.4960334973408007E-4
65-69	0.006195113072321521	1.726192496931693E-4	2.685188328560411E-4	4.373020992226955E-4	5.140217657529931E-4	1.572753163871098E-4
70-74	0.009394323166634922	1.8796318299922876E-4	2.72354816182556E-4	4.83333899140874E-4	5.792334823037458E-4	1.726192496931693E-4
75-79	0.06794677266255794	1.9179916632574364E-4	3.0687866612118985E-4	5.216937324060228E-4	6.099213489158649E-4	1.726192496931693E-4
80-84	0.09386651199981895	2.071430996318031E-4	3.145506327742196E-4	5.79233482303746E-4	7.019849487522216E-4	1.9179916632574364E-4
85-89	0.09412735886602194	2.608468662030114E-4	3.260585827537642E-4	6.214292988954095E-4	9.206359983635695E-4	1.9179916632574364E-4
90-94	0.09440738564885753	2.9920669946816014E-4	3.260585827537642E-4	6.32937248874954E-4	9.244719816900844E-4	1.9179916632574364E-4
95-99	0.09479865594816206	3.0687866612118985E-4	3.682543993454278E-4	6.674610988135879E-4	9.436518983226587E-4	1.9179916632574364E-4
100-104	0.09512471453091582	3.0687866612118985E-4	4.027782492840617E-4	6.751330654666177E-4	9.589958316287183E-4	1.9179916632574364E-4
105-109	0.09565408022997487	3.0687866612118985E-4	4.142861992636063E-4	7.134928987317663E-4	9.781757482612925E-4	1.9179916632574364E-4
110-114	0.09613357814578923	3.183866161007345E-4	4.5648201585526987E-4	7.288368320378259E-4	9.858477149143224E-4	1.9179916632574364E-4
115-119	0.09652868442842026	3.260585827537642E-4	4.7566193248784426E-4	7.480167486704002E-4	0.0010126995981999264	1.9179916632574364E-4
120-124	0.09760659574317095	3.2989456608027907E-4	4.7949791581435914E-4	7.978845319150937E-4	0.0010165355815264414	1.9179916632574364E-4
125-129	0.09939416397332687	3.452384993863386E-4	4.7949791581435914E-4	8.439163318332721E-4	0.0010357154981590158	2.071430996318031E-4
130-134	0.10125461588668658	3.452384993863386E-4	4.871698824673889E-4	8.477523151597868E-4	0.0010779113147506793	2.1097908295831803E-4
135-139	0.10323781926649478	3.6058243269239805E-4	4.986778324469335E-4	9.091280483840248E-4	0.001146959014627947	2.1097908295831803E-4
140-144	0.10495250381344694	3.6441841601891293E-4	4.986778324469335E-4	9.513238649756885E-4	0.0012198426978317297	2.1097908295831803E-4
145-149	0.10650991304401196	3.6441841601891293E-4	4.986778324469335E-4	0.0010011916482203817	0.0012505305644438486	2.1097908295831803E-4
150-154	0.10795607875810806	3.6441841601891293E-4	4.986778324469335E-4	0.001051059431465075	0.0012850544143824824	2.1097908295831803E-4
155-159	0.10948663610538752	3.6441841601891293E-4	4.986778324469335E-4	0.0010587313981181051	0.0013080703143415718	2.1097908295831803E-4
160-164	0.11117063278572754	3.6441841601891293E-4	4.986778324469335E-4	0.0010740753314241644	0.001350266130933235	2.1097908295831803E-4
165-167	0.11210661271739715	3.8359833265148727E-4	4.986778324469335E-4	0.0010740753314241644	0.0013617740809127802	2.10979082958318E-4
>>END_MODULE
