##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293586
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6232586
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.56679875736973	39.0	37.0	39.0	35.0	39.0
2	39.190531987845816	40.0	38.0	41.0	36.0	41.0
3	39.287831567827546	40.0	39.0	41.0	36.0	41.0
4	39.33191054242974	40.0	39.0	41.0	36.0	41.0
5	39.3493756845072	40.0	39.0	41.0	36.0	41.0
6	39.33531731451439	40.0	39.0	41.0	36.0	41.0
7	39.377396477160524	40.0	39.0	41.0	36.0	41.0
8	39.33977020132574	40.0	39.0	41.0	36.0	41.0
9	39.33077104752345	40.0	39.0	41.0	36.0	41.0
10-14	39.271505888566956	40.0	39.0	41.0	36.0	41.0
15-19	39.12553890150893	40.0	38.0	41.0	36.0	41.0
20-24	38.930392649214944	40.0	38.0	41.0	35.0	41.0
25-29	38.706016956685396	40.0	38.0	41.0	35.0	41.0
30-34	38.58580813806661	40.0	37.6	41.0	34.6	41.0
35-39	38.1706098880946	39.6	36.6	40.2	34.0	41.0
40-44	37.84836313530211	39.0	35.6	40.0	33.8	41.0
45-49	37.18972943173186	37.6	35.0	40.0	33.0	41.0
50-54	36.48115257454931	36.4	35.0	39.4	33.0	40.8
55-59	35.81071770209027	35.2	34.0	38.2	32.6	40.0
60-64	35.14951896371747	35.0	33.8	36.8	31.8	39.2
65-69	34.85753868458453	35.0	34.0	36.0	32.0	38.6
70-74	34.716598856397646	35.0	34.0	35.4	32.0	37.6
75-79	34.615955816734825	35.0	34.0	35.0	32.0	37.2
80-84	34.59642328240638	35.0	34.0	35.0	32.0	38.0
85-89	34.46958001060876	34.8	33.8	35.0	31.6	38.2
90-94	38.222168056044644	39.8	37.6	40.6	32.8	40.6
95-99	38.49537304211359	40.0	38.0	41.0	33.0	41.0
100-104	38.363778646666944	40.0	37.0	41.0	33.0	41.0
105-109	38.21880752856849	40.0	37.0	41.0	33.0	41.0
110-114	38.01046821430138	40.0	36.2	41.0	33.0	41.0
115-119	37.77173431730411	39.4	35.6	40.4	32.2	41.0
120-124	37.49458688898311	39.0	35.0	40.0	32.0	41.0
125-129	37.048097690145084	38.4	34.8	39.8	31.8	40.6
130-134	36.821748945330135	37.8	34.6	40.0	31.8	41.0
135-139	36.43690374688311	36.8	34.0	40.0	31.4	41.0
140-144	36.07175668983312	35.8	34.0	39.0	32.0	41.0
145-149	35.4698339339539	35.0	34.0	37.6	31.8	39.8
150-154	34.9279802076299	35.0	34.0	36.2	31.0	38.8
155-159	34.50491754899484	35.0	34.0	35.4	31.0	37.0
160-164	34.20843124848953	35.0	34.0	35.0	31.0	36.0
165-169	34.04092417389235	35.0	34.0	35.0	31.0	36.0
170-174	33.967265025214395	35.0	34.0	35.0	31.0	35.2
175-178	33.72328900812349	34.75	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	106.0
29	1435.0
30	8745.0
31	35887.0
32	116036.0
33	257909.0
34	462449.0
35	808570.0
36	1365188.0
37	1809924.0
38	1185745.0
39	179915.0
40	676.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.657623336444935	27.752412882870768	28.43590124548622	22.154062535198072
2	21.980266297167823	28.26816990571811	28.797468659076664	20.954095138037403
3	20.197378744553223	26.700762733157635	30.239309975024813	22.862548547264332
4	21.137149170504827	25.969461151438583	30.47176565233115	22.421624025725436
5	22.13217755840032	26.821178239658465	28.531287013127454	22.515357188813763
6	22.506612824917298	26.826456947405138	27.913148731521716	22.75378149615585
7	22.38576411139774	27.44225270216889	27.599394537034865	22.5725886493985
8	22.37713206043206	27.611652691194312	27.602202360304375	22.409012888069256
9	22.830844211375503	27.36520603165363	27.072390176405108	22.731559580565754
10-14	22.591001552164702	27.630072011842277	27.17885641690303	22.60007001908999
15-19	22.5419721444678	27.51893034448301	27.338722642575654	22.600374868473537
20-24	22.36511778577945	27.468912582995248	27.52968992325176	22.636279707973543
25-29	22.39691518095378	27.52764903685244	27.356015624974926	22.71942015721885
30-34	22.477488477495537	27.533357742676955	27.288964805299116	22.70018897452839
35-39	22.44839621948257	27.521885779032974	27.321564435693308	22.70815356579115
40-44	22.485388889940708	27.561160006456387	27.284308632083054	22.669142471519848
45-49	22.772319547616352	27.38304132506154	27.114767449658938	22.729871677663173
50-54	22.67925384423095	27.443661427214966	27.204415631007738	22.672669097546347
55-59	22.60804423717539	27.46017463698054	27.222956891409122	22.708824234434953
60-64	22.63352643669899	27.4460585060519	27.18124386891733	22.739171188331778
65-69	22.573262526983182	27.432128493694268	27.253422576118485	22.741186403204065
70-74	22.50899706799072	27.53076170950549	27.222931219882085	22.737310002621705
75-79	22.566620019362748	27.445281942359077	27.20496435989812	22.78313367838005
80-84	22.508281474174606	27.527883289536636	27.210451648801957	22.753383587486802
85-89	22.493658330587017	27.558332929541606	27.16227581937899	22.785732920492393
90-94	21.42077357437855	27.251932577943354	28.921752989910104	22.405540857767996
95-99	22.248292671511727	27.52774212645447	27.51670150898846	22.70726369304534
100-104	22.36843360634421	27.636503364562653	27.27063387828027	22.724429150812867
105-109	22.25887169969542	27.62374033179083	27.45819424016151	22.659193728352246
110-114	22.284505180102947	27.477711880783133	27.475932763685424	22.761850175428496
115-119	22.304865580204158	27.515589022035947	27.385819406265487	22.79372599149441
120-124	22.27455672386459	27.577736479418885	27.386089422699538	22.76161737401699
125-129	22.318188339322003	27.529210972201497	27.387715016727256	22.764885671749244
130-134	22.43520110763563	27.530818768702332	27.29667128696547	22.73730883669657
135-139	22.550965014347813	27.529506388786917	27.223656325119915	22.69587227174535
140-144	22.57336258869986	27.538793266886746	27.27538449917402	22.61245964523937
145-149	22.49020867788926	27.59342918530941	27.33716833722285	22.57919379957848
150-154	22.400088272357348	27.717070595199054	27.400506947323265	22.482334185120333
155-159	22.414002192437998	27.69056310655339	27.380578277224306	22.51485642378431
160-164	22.32624651325602	27.828053554633637	27.458309796060647	22.3873901360497
165-169	22.20735468110502	27.913416093991607	27.546424529576946	22.332804695326427
170-174	22.243905168782412	28.019931015133082	27.511187515674585	22.22497630040992
175-178	22.149451752779918	28.112955850648785	27.557409120062747	22.180183276508554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.5
13	6.0
14	11.5
15	19.5
16	46.5
17	122.5
18	291.5
19	660.0
20	1384.5
21	2704.5
22	4916.0
23	8464.0
24	13696.5
25	20627.5
26	29293.5
27	39833.0
28	51962.0
29	65011.5
30	78618.5
31	92366.0
32	106659.0
33	121497.0
34	136519.5
35	152515.0
36	168843.5
37	184510.5
38	200008.5
39	215194.0
40	229320.0
41	241784.0
42	252201.0
43	259669.0
44	263072.0
45	263286.5
46	261128.0
47	255049.0
48	245193.0
49	232556.0
50	218122.0
51	202862.5
52	187032.0
53	171135.5
54	156026.0
55	141374.5
56	127700.5
57	115595.5
58	103673.5
59	92783.0
60	82808.5
61	73566.5
62	64983.5
63	56611.5
64	48702.0
65	41390.5
66	34654.0
67	28331.0
68	22738.5
69	17986.5
70	13904.5
71	10397.5
72	7503.5
73	5243.0
74	3614.0
75	2453.5
76	1579.5
77	958.5
78	585.0
79	351.5
80	190.0
81	106.5
82	52.0
83	20.0
84	11.0
85	4.0
86	1.5
87	1.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2175.0
92-93	4810.0
94-95	7380.0
96-97	9188.0
98-99	11333.0
100-101	13336.0
102-103	14972.0
104-105	16635.0
106-107	18524.0
108-109	19812.0
110-111	21339.0
112-113	22123.0
114-115	23239.0
116-117	24266.0
118-119	25535.0
120-121	26432.0
122-123	27289.0
124-125	28315.0
126-127	29352.0
128-129	30028.0
130-131	31150.0
132-133	33438.0
134-135	120001.0
136-137	108107.0
138-139	101501.0
140-141	103955.0
142-143	101685.0
144-145	103975.0
146-147	99982.0
148-149	99635.0
150-151	104957.0
152-153	100359.0
154-155	93271.0
156-157	93342.0
158-159	88660.0
160-161	93022.0
162-163	95369.0
164-165	97707.0
166-167	97225.0
168-169	95935.0
170-171	99547.0
172-173	94918.0
174-175	107704.0
176-177	218925.0
178-179	3472133.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.43405440753075
#Duplication Level	Percentage of total
1	86.4872256341475
2	10.03588282533056
3	1.9661433724926383
4	0.5635532443988012
5	0.2866423749311836
6	0.16630990705010432
7	0.11107001848288107
8	0.06865741975066751
9	0.04578424142844258
>10	0.2672603359618425
>50	0.0014706260254029036
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.6044704397179597E-5	0.0	0.0	0.0	0.0	0.0
2	1.7649174836897557E-4	0.0	1.6044704397179597E-5	0.0	0.0	0.0
3	1.7649174836897557E-4	3.2089408794359194E-5	1.6044704397179597E-5	0.0	0.0	0.0
4	1.7649174836897557E-4	3.2089408794359194E-5	1.6044704397179597E-5	1.6044704397179597E-5	1.6044704397179597E-5	0.0
5	1.7649174836897557E-4	3.2089408794359194E-5	4.8134113191538794E-5	4.8134113191538794E-5	3.2089408794359194E-5	0.0
6	1.7649174836897557E-4	3.2089408794359194E-5	4.8134113191538794E-5	4.8134113191538794E-5	4.8134113191538794E-5	0.0
7	1.7649174836897557E-4	3.2089408794359194E-5	6.417881758871839E-5	6.417881758871839E-5	4.8134113191538794E-5	0.0
8	1.9253645276615518E-4	3.2089408794359194E-5	8.0223521985898E-5	8.0223521985898E-5	4.8134113191538794E-5	0.0
9	1.9253645276615518E-4	3.2089408794359194E-5	8.0223521985898E-5	8.0223521985898E-5	4.8134113191538794E-5	0.0
10-14	1.98954334525027E-4	3.2089408794359194E-5	1.18730812539129E-4	8.664140374476983E-5	4.8134113191538794E-5	0.0
15-19	2.1820797980164254E-4	3.2089408794359194E-5	1.4440233957461638E-4	1.6365598485123192E-4	5.134305407097471E-5	0.0
20-24	2.4067056595769397E-4	3.2089408794359194E-5	1.60447043971796E-4	1.98954334525027E-4	6.738775846815431E-5	0.0
25-29	2.7596891563148906E-4	3.2089408794359194E-5	1.7649174836897557E-4	2.2141692068107844E-4	8.985034462420576E-5	0.0
30-34	5.070126589508752E-4	3.8507290553231034E-5	1.7649174836897557E-4	2.5992421123430953E-4	1.0910398990082125E-4	0.0
35-39	0.0016205151441151394	7.701458110646208E-5	1.9253645276615518E-4	3.208940879435919E-4	1.476112804540523E-4	0.0
40-44	0.002900882555010071	8.0223521985898E-5	1.9253645276615518E-4	4.0753549168836184E-4	2.3746162507825804E-4	0.0
45-49	0.003940579399947309	8.0223521985898E-5	2.1179009804277068E-4	4.749232501565162E-4	3.5619243761738706E-4	6.417881758871838E-6
50-54	0.004842291787068803	8.664140374476983E-5	2.4708844771656577E-4	4.909679545536956E-4	4.5887854575933656E-4	1.6044704397179597E-5
55-59	0.005699079001878193	9.626822638307759E-5	2.5671527035487355E-4	5.16639481589183E-4	5.262663042274908E-4	1.6044704397179597E-5
60-64	0.006337658236885942	1.0910398990082125E-4	2.631331521137454E-4	5.358931268657985E-4	5.936540626956451E-4	1.6044704397179597E-5
65-69	0.006828626191439637	1.123129307802572E-4	2.8559573826979684E-4	5.455199495041064E-4	7.220116978730819E-4	1.6044704397179597E-5
70-74	0.01057025125686192	1.123129307802572E-4	3.0484938354641234E-4	5.455199495041064E-4	7.637279293057489E-4	3.2089408794359194E-5
75-79	0.07956248016473419	1.123129307802572E-4	3.20894087943592E-4	5.551467721424142E-4	7.95817338100108E-4	7.701458110646207E-5
80-84	0.10913287036873617	1.1873081253912902E-4	3.5940137849682294E-4	5.808182991779014E-4	9.241749732775449E-4	9.626822638307759E-5
85-89	0.10943451081140317	1.60447043971796E-4	3.6902820113513077E-4	6.096987670928247E-4	0.0012258154159445211	9.626822638307759E-5
90-94	0.10971047972703465	1.9253645276615518E-4	3.6902820113513077E-4	6.353702941283121E-4	0.0012546958838594445	9.626822638307759E-5
95-99	0.10996077711563065	2.0858115716333478E-4	3.722371420145667E-4	6.514149985254917E-4	0.0012675316473771882	9.626822638307759E-5
100-104	0.1103041337897303	2.0858115716333478E-4	4.0753549168836173E-4	6.963401708375945E-4	0.0012803674108949321	9.626822638307759E-5
105-109	0.1107694302172485	2.2141692068107844E-4	4.3962490048272095E-4	7.220116978730819E-4	0.001286785292653804	9.626822638307759E-5
110-114	0.11120905511773123	2.246258615605144E-4	4.492517231210288E-4	7.284295796319536E-4	0.0013445462284836503	9.626822638307759E-5
115-119	0.1116165906094196	2.3104374331938622E-4	4.749232501565161E-4	7.733547519440567E-4	0.0013798445781574454	9.626822638307759E-5
120-124	0.11299322624669761	2.4067056595769397E-4	4.973858363125675E-4	8.503693330505187E-4	0.0014215608095901125	9.626822638307759E-5
125-129	0.11481911360709665	2.502973885960017E-4	5.134305407097471E-4	9.049213280009294E-4	0.0014729038636610869	9.626822638307759E-5
130-134	0.11676052283915536	2.7596891563148906E-4	5.19848422468619E-4	9.17757091518673E-4	0.0014953664498171385	9.626822638307759E-5
135-139	0.11901961721827825	2.8880467914923276E-4	5.294752451069267E-4	9.370107367952885E-4	0.0016108883214768315	9.626822638307759E-5
140-144	0.12105408573584062	2.8880467914923276E-4	5.294752451069267E-4	9.658912047102118E-4	0.0016943207843421657	9.626822638307759E-5
145-149	0.12283504792392756	2.8880467914923276E-4	5.455199495041064E-4	9.883537908662633E-4	0.0017167833704982171	9.626822638307759E-5
150-154	0.12442668260012776	2.8880467914923276E-4	5.455199495041064E-4	0.0010364879040578021	0.0017232012522570886	9.626822638307759E-5
155-159	0.12591884010906548	2.8880467914923276E-4	5.551467721424142E-4	0.001087830958128777	0.001790589010725243	9.626822638307759E-5
160-164	0.12768375759275524	3.0484938354641234E-4	5.840272400573373E-4	0.001123129307802572	0.0018451410056756538	9.626822638307759E-5
165-167	0.12878014572656252	3.0484938354641234E-4	5.936540626956451E-4	0.0011391740121997514	0.0018611857100728332	9.626822638307759E-5
>>END_MODULE
