##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293587
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5578787
Total Bases	919 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.50243646154621	39.0	37.0	39.0	35.0	39.0
2	39.110099202568584	40.0	38.0	41.0	36.0	41.0
3	39.21709701410002	40.0	38.0	41.0	36.0	41.0
4	39.27241369853339	40.0	39.0	41.0	36.0	41.0
5	39.302328445233705	40.0	39.0	41.0	36.0	41.0
6	39.297355141897334	40.0	39.0	41.0	36.0	41.0
7	39.33803800001685	40.0	39.0	41.0	36.0	41.0
8	39.31280401994197	40.0	39.0	41.0	36.0	41.0
9	39.29919819487641	40.0	39.0	41.0	36.0	41.0
10-14	39.21545113660012	40.0	38.8	41.0	36.0	41.0
15-19	39.076753279879654	40.0	38.0	41.0	36.0	41.0
20-24	38.89298863713563	40.0	38.0	41.0	35.0	41.0
25-29	38.65971835813053	40.0	38.0	40.8	35.0	41.0
30-34	38.53103934600837	40.0	37.6	40.8	34.4	41.0
35-39	38.116218669040414	39.6	36.6	40.0	33.8	41.0
40-44	37.78671947862502	39.0	35.6	40.0	33.6	41.0
45-49	37.12736209502173	37.6	35.0	40.0	33.0	41.0
50-54	36.41391155460856	36.4	34.6	39.2	33.0	40.6
55-59	35.74153876102458	35.2	34.0	38.0	32.2	40.0
60-64	35.07416959995067	35.0	33.8	36.8	31.8	39.2
65-69	34.787869800370586	35.0	33.8	36.0	32.0	38.6
70-74	34.65376627571549	35.0	34.0	35.4	32.0	37.4
75-79	34.55129450183347	35.0	33.8	35.0	32.0	37.2
80-84	34.524104361754624	35.0	33.0	35.0	32.0	38.0
85-89	34.399784003225065	34.8	33.0	35.0	31.6	38.2
90-94	37.9063525736394	39.8	36.6	40.6	32.4	40.6
95-99	38.2362719656098	40.0	37.0	41.0	33.0	41.0
100-104	38.1201770601091	40.0	36.8	41.0	32.8	41.0
105-109	37.961289527741194	40.0	36.0	41.0	32.0	41.0
110-114	37.76464458238331	40.0	35.6	40.6	32.0	41.0
115-119	37.51627659225859	39.0	35.0	40.0	32.0	41.0
120-124	37.25789017663084	38.8	35.0	40.0	32.0	41.0
125-129	36.83007597547101	38.0	34.6	39.8	31.4	40.6
130-134	36.5969702345736	37.4	34.0	40.0	31.0	41.0
135-139	36.23847690205686	36.2	34.0	40.0	31.0	41.0
140-144	35.874868655168484	35.6	34.0	39.0	31.0	40.8
145-149	35.30340995247245	35.0	34.0	37.2	31.0	39.8
150-154	34.771595693352076	35.0	34.0	36.2	31.0	38.8
155-159	34.37100389932628	35.0	33.8	35.4	31.0	37.0
160-164	34.08494458391493	35.0	33.0	35.0	31.0	36.0
165-169	33.91993820880164	35.0	33.0	35.0	31.0	36.0
170-174	33.848229248718795	35.0	33.0	35.0	31.0	35.0
175-178	33.617457606792556	34.75	32.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	6.0
28	117.0
29	1616.0
30	9829.0
31	41087.0
32	128558.0
33	272651.0
34	454529.0
35	756966.0
36	1228099.0
37	1561855.0
38	979572.0
39	143256.0
40	646.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.595070756420707	27.760532890035055	28.507702480843953	22.136693872700285
2	21.93702681245941	28.28869429859932	28.88626147583695	20.88801741310432
3	20.159955918732873	26.727333379101943	30.285454526225863	22.827256175939322
4	21.10951717640412	25.99398399687961	30.537695739235073	22.358803087481203
5	22.10713547586599	26.80299498797857	28.58169706066928	22.50817247548616
6	22.510413105931452	26.836640294745077	27.94309945871746	22.709847140606012
7	22.375025251905118	27.46813957944621	27.61693536605717	22.539899802591496
8	22.380510315235195	27.610356875069797	27.616218364314683	22.39291444538033
9	22.77893025849526	27.396134679456303	27.112435014995196	22.71250004705324
10-14	22.537006700560532	27.67522402271318	27.212456758073035	22.57531251865325
15-19	22.50965666909312	27.573599780740864	27.36019138210511	22.556552168060907
20-24	22.331116065194816	27.488075812896245	27.56693524954439	22.61387287236455
25-29	22.35795344041635	27.567888861861906	27.394492745465993	22.679664952255752
30-34	22.4304315615563	27.579138619201633	27.344621689266862	22.645808129975208
35-39	22.407168440021103	27.56803226221041	27.339416256616357	22.685383041152136
40-44	22.461499247058544	27.59887050715505	27.30999767512185	22.629632570664555
45-49	22.73700716661167	27.416300353463935	27.141269240069573	22.70542323985483
50-54	22.65718694762858	27.47352426253234	27.23501004788317	22.634278741955914
55-59	22.572752822432545	27.493442570938807	27.256555950245097	22.677248656383547
60-64	22.59379323856602	27.47556054748102	27.22982612528494	22.70082008866802
65-69	22.514682851308	27.47350992249749	27.285508480606985	22.726298745587524
70-74	22.46893097011949	27.561446601205603	27.264658069935276	22.70496435873963
75-79	22.514765306508387	27.50445930271222	27.236429711333304	22.744345679446088
80-84	22.454719995583268	27.566827699283014	27.23824372574181	22.7402085793919
85-89	22.447973009186406	27.5932205334242	27.190451974595913	22.76835448279348
90-94	21.38141917418026	27.283952981847083	28.924350321866594	22.410277522106067
95-99	22.183060967826012	27.56337111877489	27.57698738610717	22.676580527291932
100-104	22.309531694516803	27.673324254902496	27.34235696682093	22.674787083759764
105-109	22.191363265659724	27.657555490884366	27.499847573765653	22.651233669690257
110-114	22.235580047976537	27.512775211074292	27.508111519084196	22.743533221864976
115-119	22.265114698134855	27.573320111704756	27.397846170887004	22.763719019273385
120-124	22.228063677423396	27.61734830395197	27.423498376935555	22.731089641689074
125-129	22.28152657631314	27.591578970890378	27.40309089584508	22.723803556951403
130-134	22.3982887581185	27.569703678902624	27.334497317110003	22.69751024586887
135-139	22.512759519224694	27.550280404752662	27.28443256581779	22.652527510204852
140-144	22.543658987189296	27.584786055084937	27.297804998447617	22.57374995927815
145-149	22.471074261043704	27.617725768993758	27.365988516364638	22.5452114535979
150-154	22.371726310107924	27.720102777025513	27.437326147873005	22.47084476499356
155-159	22.378964944481446	27.73599753154509	27.425217822655114	22.459819701318352
160-164	22.28779844408524	27.885384461629936	27.475138803675414	22.35167829060941
165-169	22.18828940097921	27.942795809406423	27.561132557897807	22.307782231716562
170-174	22.21212469425612	28.02588320621054	27.538627073797876	22.223365025735465
175-178	22.147689485612457	28.147155607073447	27.560388467096214	22.144766440217882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	1.0
12	2.5
13	6.0
14	11.5
15	20.0
16	47.0
17	108.0
18	269.5
19	591.5
20	1228.5
21	2448.0
22	4450.0
23	7644.0
24	12250.0
25	18408.0
26	26296.0
27	35931.5
28	46812.5
29	58381.5
30	70580.5
31	83545.0
32	96495.0
33	109745.0
34	123464.5
35	137365.5
36	151964.0
37	166311.0
38	180361.0
39	193859.5
40	206795.5
41	217956.0
42	226928.5
43	233651.5
44	236899.0
45	237558.0
46	234166.0
47	227237.5
48	218696.5
49	207863.5
50	194869.5
51	180787.0
52	166342.0
53	152761.5
54	139035.0
55	125358.0
56	113142.5
57	101804.5
58	91360.0
59	81901.5
60	73238.5
61	64987.5
62	57084.0
63	49767.5
64	42933.5
65	36264.0
66	30236.5
67	24975.5
68	20126.5
69	15754.5
70	12117.5
71	9065.0
72	6645.0
73	4671.5
74	3145.0
75	2112.5
76	1390.5
77	861.0
78	501.0
79	290.0
80	166.0
81	87.5
82	46.0
83	26.5
84	14.5
85	4.5
86	2.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2024.0
92-93	4474.0
94-95	6683.0
96-97	8714.0
98-99	10819.0
100-101	12563.0
102-103	14212.0
104-105	15894.0
106-107	17568.0
108-109	18844.0
110-111	20485.0
112-113	21198.0
114-115	21990.0
116-117	23166.0
118-119	24426.0
120-121	25193.0
122-123	27143.0
124-125	26894.0
126-127	27364.0
128-129	28834.0
130-131	29490.0
132-133	31497.0
134-135	112472.0
136-137	104769.0
138-139	96607.0
140-141	98382.0
142-143	104024.0
144-145	104496.0
146-147	88706.0
148-149	97255.0
150-151	104204.0
152-153	101424.0
154-155	92531.0
156-157	85354.0
158-159	84043.0
160-161	81497.0
162-163	92806.0
164-165	84459.0
166-167	90288.0
168-169	87835.0
170-171	97745.0
172-173	98452.0
174-175	99084.0
176-177	188526.0
178-179	2964353.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.06291500548409
#Duplication Level	Percentage of total
1	87.5420466418947
2	9.429662451105196
3	1.71265652756427
4	0.5015450785287153
5	0.21837464004027285
6	0.1409808216934509
7	0.09061897260543587
8	0.054221704644938765
9	0.0375440604652321
>10	0.26752135177164216
>50	0.004827749686137248
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.7925043562337116E-5	0.0	0.0	0.0	0.0	0.0
2	3.585008712467423E-4	0.0	0.0	0.0	0.0	0.0
3	3.585008712467423E-4	0.0	0.0	1.7925043562337116E-5	0.0	0.0
4	3.585008712467423E-4	0.0	0.0	1.7925043562337116E-5	0.0	0.0
5	3.585008712467423E-4	0.0	1.7925043562337116E-5	5.377513068701135E-5	0.0	0.0
6	3.585008712467423E-4	1.7925043562337116E-5	1.7925043562337116E-5	7.170017424934847E-5	0.0	0.0
7	3.585008712467423E-4	1.7925043562337116E-5	1.7925043562337116E-5	7.170017424934847E-5	0.0	0.0
8	3.585008712467423E-4	1.7925043562337116E-5	1.7925043562337116E-5	7.170017424934847E-5	0.0	0.0
9	3.585008712467423E-4	1.7925043562337116E-5	1.7925043562337116E-5	8.962521781168558E-5	0.0	0.0
10-14	3.800109235215469E-4	1.7925043562337116E-5	3.585008712467423E-5	1.0396525266155526E-4	0.0	0.0
15-19	4.302010454960908E-4	1.7925043562337116E-5	3.943509583714165E-5	1.398153397862295E-4	0.0	0.0
20-24	4.6605113262076505E-4	2.509506098727196E-5	5.377513068701135E-5	1.5774038334856664E-4	0.0	0.0
25-29	4.91146193608037E-4	5.377513068701135E-5	5.377513068701135E-5	1.9000546176077345E-4	3.5850087124674226E-5	0.0
30-34	8.460620561423118E-4	5.377513068701135E-5	5.377513068701135E-5	2.2944055759791508E-4	5.377513068701135E-5	0.0
35-39	0.0020721350358061707	5.377513068701135E-5	7.170017424934847E-5	2.5095060987271966E-4	6.453015682441362E-5	0.0
40-44	0.003581423703754956	5.736013939947877E-5	8.604020909921817E-5	2.5095060987271966E-4	1.1113527008649012E-4	0.0
45-49	0.004807496683418814	7.170017424934847E-5	1.0396525266155526E-4	2.688756534350568E-4	1.8642045304830603E-4	0.0
50-54	0.00573242893123541	7.170017424934847E-5	1.4340034849869696E-4	2.832156882849264E-4	2.760456708599916E-4	0.0
55-59	0.006503205804415907	7.170017424934847E-5	2.1151551403557795E-4	2.8680069699739386E-4	3.04725740559731E-4	7.170017424934846E-6
60-64	0.007144922363947576	8.962521781168558E-5	2.3661057502284995E-4	3.0831074927219844E-4	3.656708886716772E-4	1.7925043562337116E-5
65-69	0.007578708418156133	9.321022652415302E-5	2.5453561858518705E-4	3.369908189719378E-4	4.1586101064622116E-4	1.7925043562337116E-5
70-74	0.011360892609809265	1.075502613740227E-4	2.688756534350568E-4	3.5133085382180754E-4	4.732211500456999E-4	1.7925043562337116E-5
75-79	0.08183499387949388	1.075502613740227E-4	2.79630679572459E-4	3.907659496589492E-4	5.377513068701136E-4	2.509506098727196E-5
80-84	0.11178415666344672	1.075502613740227E-4	2.903857057098613E-4	4.3737106292102563E-4	6.77566646656343E-4	3.585008712467423E-5
85-89	0.11212473249113113	1.362303310737621E-4	3.04725740559731E-4	4.6963614133323244E-4	8.89082160691921E-4	3.585008712467423E-5
90-94	0.11241870320555347	1.4340034849869693E-4	3.04725740559731E-4	5.198262633077764E-4	9.177622303916603E-4	3.585008712467423E-5
95-99	0.11283814922491216	1.4698535721116435E-4	3.334058102594704E-4	5.41336315582581E-4	9.536123175163346E-4	3.585008712467423E-5
100-104	0.11331495538367031	1.6132539206103406E-4	3.7642591480907946E-4	5.736013939947877E-4	0.001000217430778411	3.585008712467423E-5
105-109	0.11375949646401629	1.6132539206103406E-4	4.051059845088189E-4	5.986964549820597E-4	0.0010504075527529551	4.6605113262076494E-5
110-114	0.11431517281444874	1.7566542691090377E-4	4.302010454960908E-4	6.883216727937454E-4	0.0010755026137402271	5.377513068701135E-5
115-119	0.11485292412131884	1.8642045304830603E-4	4.302010454960908E-4	7.205867512059521E-4	0.001093427657302564	5.377513068701135E-5
120-124	0.1161471122665196	1.971754791857083E-4	4.302010454960908E-4	7.779468906054309E-4	0.0011292777444272383	5.377513068701135E-5
125-129	0.11806150691897718	2.0793050532311053E-4	4.302010454960908E-4	8.066269603051703E-4	0.00116871284026438	5.377513068701135E-5
130-134	0.12001175165855946	2.151005227480454E-4	4.302010454960908E-4	8.532320735672467E-4	0.0011973929099641195	5.377513068701135E-5
135-139	0.12229540220840121	2.151005227480454E-4	4.302010454960908E-4	9.070072042542581E-4	0.0012798481103508702	5.377513068701135E-5
140-144	0.12453961766240582	2.2227054017298024E-4	4.445410803459605E-4	9.213472391041278E-4	0.0014053234152872302	5.377513068701135E-5
145-149	0.12631061196636473	2.3302556631038253E-4	4.481260890584279E-4	9.500273088038672E-4	0.001498533641811383	5.377513068701135E-5
150-154	0.12792386588697507	2.4019558373531737E-4	4.6963614133323244E-4	9.57197326228802E-4	0.0015057036592363178	5.377513068701135E-5
155-159	0.12954428982501037	2.5095060987271966E-4	5.019012197454393E-4	9.966324220659437E-4	0.0015451387550734596	5.377513068701135E-5
160-164	0.13139415432064355	2.688756534350568E-4	5.198262633077764E-4	0.0010038024394908786	0.0016383489815976124	5.736013939947877E-5
165-167	0.13261544728869076	2.688756534350568E-4	5.377513068701136E-4	0.0010157524685324368	0.001690929109380468	7.170017424934847E-5
>>END_MODULE
