##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293589
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4487796
Total Bases	718.8 Mbp
Sequences flagged as poor quality	0
Sequence length	90-172
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.137349826061616	40.0	39.0	41.0	36.0	41.0
2	39.34236115010575	40.0	39.0	41.0	36.0	41.0
3	39.38703586348399	40.0	39.0	41.0	37.0	41.0
4	39.414307156564156	40.0	39.0	41.0	37.0	41.0
5	39.38285407803742	40.0	39.0	41.0	37.0	41.0
6	39.38784450095325	40.0	39.0	41.0	37.0	41.0
7	39.374215984862055	40.0	39.0	41.0	37.0	41.0
8	39.35101706940333	40.0	39.0	41.0	36.0	41.0
9	39.320240715041415	40.0	39.0	41.0	36.0	41.0
10-14	39.22560758109326	40.0	38.8	41.0	36.0	41.0
15-19	39.05531245181377	40.0	38.0	41.0	35.8	41.0
20-24	38.85231579153776	40.0	38.0	41.0	35.0	41.0
25-29	38.68588893078028	40.0	37.8	40.6	35.0	41.0
30-34	38.25656286515697	39.8	36.8	40.0	34.0	41.0
35-39	37.91635818562163	39.0	35.8	40.0	34.0	41.0
40-44	37.22400300726682	37.8	35.0	40.0	33.0	41.0
45-49	36.41681689631169	36.4	34.6	39.4	33.0	40.6
50-54	35.69470488409009	35.2	34.0	37.8	32.4	40.0
55-59	35.000972058444724	35.0	33.8	36.6	31.8	39.0
60-64	34.68380175926	35.0	33.8	36.0	32.0	37.8
65-69	34.52566582794761	35.0	33.4	35.0	32.0	37.0
70-74	34.429015445443596	35.0	33.0	35.0	32.0	37.0
75-79	34.40078938525727	35.0	33.0	35.0	32.0	37.0
80-84	34.28770576915707	34.8	33.0	35.0	31.6	37.6
85-89	37.94883983140054	40.0	36.4	41.0	31.6	41.0
90-94	38.063276051322646	40.0	36.6	41.0	32.0	41.0
95-99	37.94656134104348	40.0	36.0	41.0	32.0	41.0
100-104	37.776136273113295	40.0	36.0	41.0	32.0	41.0
105-109	37.55882030524689	39.6	35.0	40.2	31.0	41.0
110-114	37.31729073051747	39.0	35.0	40.0	31.0	41.0
115-119	37.0607927341362	38.6	34.4	40.0	31.0	41.0
120-124	36.54715893653046	37.6	34.0	39.8	30.4	40.6
125-129	36.33162957302476	36.8	34.0	40.0	30.0	41.0
130-134	35.97159201395336	36.0	33.8	39.8	30.6	41.0
135-139	35.574513600323286	35.2	33.8	38.8	30.4	40.6
140-144	35.02912417213202	35.0	33.0	37.0	30.0	39.6
145-149	34.54033494746548	35.0	33.0	36.0	30.0	38.4
150-154	34.15351642422895	35.0	33.0	35.2	30.0	36.8
155-159	33.886441648480556	35.0	33.0	35.0	30.0	36.0
160-164	33.7283550117109	35.0	33.0	35.0	30.0	35.8
165-169	33.66023797369975	35.0	33.0	35.0	30.0	35.0
170-172	33.37984414635312	34.666666666666664	32.666666666666664	35.0	29.666666666666668	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	2.0
28	124.0
29	1657.0
30	10540.0
31	45089.0
32	140067.0
33	263378.0
34	385878.0
35	614121.0
36	991817.0
37	1211049.0
38	721612.0
39	101991.0
40	471.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.047861355551813	27.01573333547247	27.541314266513005	22.395091042462713
2	22.33871593093804	27.240342475460118	28.09350068496875	22.3274409086331
3	22.68957858155763	27.125898770799743	27.220600045100092	22.96392260254254
4	23.099534827340637	27.439794500463034	27.027921946541245	22.432748725655088
5	22.468601513972562	27.68062541167201	27.540133285915847	22.31063978843958
6	22.82993701139713	27.314432295942147	27.00833549475065	22.847295197910064
7	23.281027034205653	27.434981447463297	26.86347596905029	22.42051554928076
8	22.478962947513654	27.69620098596282	27.39264440718785	22.432191659335672
9	22.665179076767302	27.344848116982146	26.98774632358512	23.002226482665435
10-14	22.534678492516147	27.649656980843158	27.43592177541047	22.379742751230225
15-19	22.532401205402387	27.412155097958994	27.492421669790694	22.563022026847925
20-24	22.397381699168147	27.50821561407871	27.468681731522555	22.625720955230584
25-29	22.48583046109939	27.62666573970831	27.456016271684362	22.431487527507933
30-34	22.488580140452015	27.50990018262862	27.32825199719417	22.673267679725193
35-39	22.357050097642585	27.602943627562393	27.54089089611025	22.49911537868477
40-44	22.526973151186017	27.56699725210326	27.398968224045834	22.50706137266489
45-49	22.71203503902584	27.511415403017423	27.252838587137205	22.52371097081953
50-54	22.567237904753245	27.500131467651386	27.352829763206703	22.579800864388666
55-59	22.50410669290672	27.613157995595166	27.41178966245346	22.470945649044655
60-64	22.555338968170567	27.537254367177116	27.30922706825355	22.59817959639877
65-69	22.397047459376495	27.60925853135927	27.399690181995794	22.59400382726844
70-74	22.382541452418963	27.658610150728773	27.42246750966399	22.536380887188276
75-79	22.408719112900854	27.642415118690778	27.26817796530858	22.680687803099783
80-84	22.314744253081024	27.66219320129525	27.394012562068333	22.6290499835554
85-89	21.61904418115262	27.142089346307184	28.765906471684545	22.472960000855654
90-94	22.21851028385762	27.536638511022183	27.45366160402462	22.791189601095574
95-99	22.267168331216247	27.68602242758245	27.344070249127967	22.70273899207334
100-104	22.155628083756	27.635475069094817	27.536405028579313	22.67249181856987
105-109	22.113272527292573	27.482220194379607	27.592240929404387	22.812266348923433
110-114	22.125913752270847	27.638301121690034	27.547458133221493	22.68832699281763
115-119	22.221315901658713	27.590290894683704	27.38861973674344	22.799773466914143
120-124	22.06963796441613	27.615956393482477	27.583552508255245	22.73085313384615
125-129	22.28218074422864	27.669237872214076	27.42593288151508	22.62264850204221
130-134	22.449207518845437	27.59565109864682	27.2900694077368	22.665071974770946
135-139	22.337659589594885	27.650873567928862	27.447797755463338	22.563669087012915
140-144	22.286143816828154	27.738096493469445	27.478745452406926	22.497014237295467
145-149	22.2526158078415	27.78947026621163	27.470583348784782	22.487330577162087
150-154	22.143806635678608	27.83521510929252	27.570406335275333	22.450571919753536
155-159	22.07687961911736	27.956338391473672	27.646028207810218	22.320753781598746
160-164	22.05968474073057	28.015877946438533	27.547509417448236	22.37692789538266
165-169	21.889088626429086	28.141170278457473	27.72900226001124	22.24073883510221
170-172	21.915840330603274	28.246738264084424	27.680023781850156	22.157397623462145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	6.5
16	24.0
17	59.0
18	125.5
19	290.5
20	613.0
21	1259.5
22	2418.5
23	4164.0
24	6868.5
25	10638.5
26	15655.0
27	21936.0
28	29416.5
29	38343.0
30	48309.5
31	59488.0
32	71825.0
33	84735.0
34	98313.5
35	112317.5
36	126614.5
37	140279.5
38	153567.0
39	165989.0
40	176678.0
41	185924.0
42	193100.0
43	197636.0
44	199575.5
45	198113.5
46	194407.5
47	188532.0
48	179955.0
49	170387.0
50	159873.0
51	148106.5
52	135326.0
53	122472.0
54	110091.0
55	98888.5
56	88617.0
57	78818.0
58	69919.5
59	61842.5
60	54621.5
61	48255.0
62	42292.0
63	36865.0
64	31665.0
65	26774.5
66	22387.5
67	18369.5
68	14763.0
69	11495.0
70	8649.5
71	6443.0
72	4798.0
73	3472.5
74	2359.5
75	1534.0
76	1005.5
77	640.0
78	381.0
79	220.5
80	131.0
81	75.5
82	37.5
83	20.0
84	9.0
85	4.5
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-172	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1518.0
92-93	3292.0
94-95	4944.0
96-97	6597.0
98-99	8321.0
100-101	9875.0
102-103	10853.0
104-105	12101.0
106-107	13315.0
108-109	14252.0
110-111	15270.0
112-113	16603.0
114-115	16980.0
116-117	17937.0
118-119	18668.0
120-121	19382.0
122-123	20378.0
124-125	20977.0
126-127	21701.0
128-129	51690.0
130-131	75166.0
132-133	70145.0
134-135	82273.0
136-137	85093.0
138-139	87710.0
140-141	82825.0
142-143	87413.0
144-145	90711.0
146-147	73547.0
148-149	73825.0
150-151	77796.0
152-153	73872.0
154-155	89296.0
156-157	75145.0
158-159	71697.0
160-161	72852.0
162-163	75657.0
164-165	73546.0
166-167	75720.0
168-169	73327.0
170-171	152514.0
172-173	2463012.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.74407482292932
#Duplication Level	Percentage of total
1	81.42665045261997
2	9.052733585627525
3	2.5554219707670662
4	1.317912902267082
5	0.8751461073465359
6	0.6493996478364502
7	0.5275055334612347
8	0.4531022130234762
9	0.36069408261990393
>10	2.344373772929841
>50	0.2783620446997903
>100	0.14596548146121824
>500	0.012732205339853917
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	2.228265277655223E-5	0.0	0.0	0.0
2	0.0	0.0	2.228265277655223E-5	0.0	0.0	0.0
3	0.0	0.0	4.456530555310446E-5	0.0	0.0	0.0
4	0.0	0.0	6.68479583296567E-5	0.0	0.0	0.0
5	0.0	0.0	8.913061110620893E-5	0.0	0.0	0.0
6	0.0	0.0	8.913061110620893E-5	0.0	0.0	0.0
7	0.0	0.0	1.1141326388276115E-4	0.0	0.0	0.0
8	0.0	0.0	1.1141326388276115E-4	2.228265277655223E-5	0.0	0.0
9	2.228265277655223E-5	0.0	1.1141326388276115E-4	2.228265277655223E-5	0.0	0.0
10-14	4.9021836108414915E-5	1.336959166593134E-5	1.1141326388276115E-4	4.456530555310446E-5	0.0	0.0
15-19	6.68479583296567E-5	2.228265277655223E-5	1.1141326388276115E-4	5.347836666372536E-5	0.0	0.0
20-24	6.68479583296567E-5	7.130448888496714E-5	1.1141326388276115E-4	8.913061110620893E-5	2.2282652776552235E-5	0.0
25-29	6.68479583296567E-5	8.913061110620893E-5	1.1141326388276115E-4	8.913061110620893E-5	4.456530555310446E-5	0.0
30-34	1.2032632499338206E-4	1.336959166593134E-4	1.3815244721462381E-4	1.0695673332745072E-4	6.239142777434627E-5	0.0
35-39	4.233704027544924E-4	2.1836999721021186E-4	1.7826122221241785E-4	1.247828555486925E-4	9.804367221682982E-5	0.0
40-44	8.556538666196058E-4	2.228265277655223E-4	1.7826122221241785E-4	1.336959166593134E-4	1.2923938610400295E-4	0.0
45-49	0.0011631544749360265	2.228265277655223E-4	1.7826122221241785E-4	1.5152203888055518E-4	1.6489163054648652E-4	0.0
50-54	0.0013503287582590652	2.228265277655223E-4	2.050004055442805E-4	2.2728305832083275E-4	1.7826122221241785E-4	0.0
55-59	0.001640003244354244	2.228265277655223E-4	2.228265277655223E-4	3.2087019998235214E-4	1.7826122221241785E-4	0.0
60-64	0.0018851124248963186	2.2728305832083275E-4	2.4065264998676406E-4	4.679357083075968E-4	2.1391346665490144E-4	0.0
65-69	0.0029725058803920677	2.4510918054207454E-4	2.89674486095179E-4	5.214140749713222E-4	2.3619611943145365E-4	0.0
70-74	0.023971677857014893	2.584787722080059E-4	2.89674486095179E-4	5.838055027456684E-4	2.89674486095179E-4	0.0
75-79	0.03323680488150531	2.807614249845581E-4	2.89674486095179E-4	6.194577471881521E-4	3.565224444248357E-4	0.0
80-84	0.0332724571259478	2.985875472057999E-4	2.985875472057999E-4	6.461969305200147E-4	5.39240197192564E-4	0.0
85-89	0.0333170224315009	3.1195713887173125E-4	3.1195713887173125E-4	6.952187666284296E-4	5.570663194138058E-4	0.0
90-94	0.03338832692038586	3.1195713887173125E-4	3.1195713887173125E-4	7.130448888496714E-4	5.748924416350476E-4	0.0
95-99	0.033490827123158005	3.1195713887173125E-4	3.2532673053766256E-4	7.620667249580864E-4	5.79348972190358E-4	0.0
100-104	0.03368245793703635	3.3423979164828345E-4	3.342397916482835E-4	8.422842749536743E-4	5.79348972190358E-4	0.0
105-109	0.03380278426202974	3.7880509720138796E-4	3.342397916482835E-4	8.823930499514684E-4	6.060881555222207E-4	0.0
110-114	0.033967675892576224	3.7880509720138796E-4	3.5652244442483576E-4	9.091322332833312E-4	6.417403999647043E-4	0.0
115-119	0.03409245874812491	3.7880509720138796E-4	3.7880509720138796E-4	9.715236610576772E-4	6.68479583296567E-4	0.0
120-124	0.034404415886996643	3.7880509720138796E-4	4.0108774997794016E-4	9.848932527236087E-4	6.68479583296567E-4	0.0
125-129	0.03499713445085294	3.7880509720138796E-4	4.0108774997794016E-4	0.001016088966610782	7.264144805156027E-4	0.0
130-134	0.035607679136930456	3.7880509720138796E-4	4.0108774997794016E-4	0.0010428281499426447	7.754363166240178E-4	0.0
135-139	0.0359196362758022	3.7880509720138796E-4	4.0108774997794016E-4	0.001087393455495749	7.843493777346385E-4	0.0
140-144	0.03618257157856551	3.832616277566984E-4	4.100008110885611E-4	0.0011319587610488535	8.066320305111908E-4	2.228265277655223E-5
145-149	0.03642322422855228	4.144573416438715E-4	4.6347917775228645E-4	0.0011898936582678892	8.422842749536743E-4	4.010877499779402E-5
150-154	0.03681094238686429	4.233704027544924E-4	4.9913142219477E-4	0.0012300024332656833	8.690234582855369E-4	4.456530555310446E-5
155-159	0.03722094319795285	4.233704027544924E-4	5.125010138607014E-4	0.001247828555486925	8.690234582855369E-4	4.456530555310446E-5
160-161	0.03750170462293741	4.233704027544924E-4	5.125010138607014E-4	0.001247828555486925	8.801647846738131E-4	4.456530555310446E-5
>>END_MODULE
