##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293590
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4802370
Total Bases	770 Mbp
Sequences flagged as poor quality	0
Sequence length	90-172
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.14602331765357	40.0	39.0	41.0	36.0	41.0
2	39.36345429444212	40.0	39.0	41.0	36.0	41.0
3	39.40836378704681	40.0	39.0	41.0	37.0	41.0
4	39.43348929799245	40.0	39.0	41.0	37.0	41.0
5	39.416561822600094	40.0	39.0	41.0	37.0	41.0
6	39.39498560085958	40.0	39.0	41.0	37.0	41.0
7	39.38231519020817	40.0	39.0	41.0	37.0	41.0
8	39.35012296012177	40.0	39.0	41.0	36.0	41.0
9	39.33873150132122	40.0	39.0	41.0	36.0	41.0
10-14	39.255641068888906	40.0	38.8	41.0	36.0	41.0
15-19	39.08497912489041	40.0	38.0	41.0	35.8	41.0
20-24	38.86347807436745	40.0	38.0	41.0	35.0	41.0
25-29	38.70724400660507	40.0	37.8	41.0	35.0	41.0
30-34	38.2966174617949	39.8	36.8	40.2	34.0	41.0
35-39	37.96384002065646	39.0	35.8	40.0	34.0	41.0
40-44	37.28850692470592	37.8	35.0	40.0	33.0	41.0
45-49	36.49495786455437	36.4	34.8	39.4	33.0	40.8
50-54	35.788387483679934	35.2	34.0	38.2	32.6	40.0
55-59	35.09794476477239	35.0	33.8	36.6	31.8	39.2
60-64	34.78651644916989	35.0	33.8	36.0	32.0	38.6
65-69	34.62669373663421	35.0	34.0	35.2	32.0	37.0
70-74	34.50868833513452	35.0	33.6	35.0	32.0	37.0
75-79	34.47763591726586	35.0	33.0	35.0	32.0	37.0
80-84	34.36374423461749	34.8	33.0	35.0	31.6	38.0
85-89	38.106978804215416	40.0	36.8	41.0	32.4	41.0
90-94	38.235795261692644	40.0	37.0	41.0	33.0	41.0
95-99	38.114268959994774	40.0	36.8	41.0	32.0	41.0
100-104	37.949473437801025	40.0	36.0	41.0	32.0	41.0
105-109	37.73623988260762	40.0	35.6	40.6	32.0	41.0
110-114	37.48811715748116	39.0	35.0	40.0	32.0	41.0
115-119	37.234505510986125	39.0	35.0	40.0	31.6	41.0
120-124	36.72365707624276	38.0	34.4	39.8	31.0	40.6
125-129	36.51795066504071	37.4	34.0	40.0	31.0	41.0
130-134	36.14022345002478	36.0	34.0	39.8	31.0	41.0
135-139	35.71716226609412	35.2	34.0	38.8	31.0	40.6
140-144	35.15466822457789	35.0	34.0	37.2	31.0	39.6
145-149	34.64153274812843	35.0	33.0	36.0	30.4	38.6
150-154	34.25131927219324	35.0	33.0	35.2	30.0	36.8
155-159	33.9757869825114	35.0	33.0	35.0	30.0	36.0
160-164	33.815783348084054	35.0	33.0	35.0	30.0	36.0
165-169	33.752016672905484	35.0	33.0	35.0	30.0	35.0
170-172	33.468357111349796	34.666666666666664	32.666666666666664	35.0	29.666666666666668	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	3.0
28	95.0
29	1501.0
30	9383.0
31	40973.0
32	127445.0
33	251061.0
34	393967.0
35	643834.0
36	1052790.0
37	1323245.0
38	829841.0
39	127263.0
40	969.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.057302956665147	26.988486934576052	27.524534760961778	22.429675347797026
2	22.334055893236048	27.256188090463667	28.07053600618028	22.339220010120002
3	22.69764720336001	27.09237314076175	27.215291616431053	22.99468803944719
4	23.116586185570874	27.379335619704438	27.02944587776452	22.474632316960168
5	22.439087367279072	27.66138385838659	27.51897500609074	22.380553768243598
6	22.80757209461162	27.29762596384702	27.016993692697568	22.8778082488438
7	23.267657427478518	27.438577202506266	26.77777847187951	22.51598689813571
8	22.51450846144716	27.655324350268724	27.36182343301328	22.468343755270833
9	22.64400702153312	27.346664251192642	26.971037217040756	23.03829151023349
10-14	22.522583640993926	27.62268213402966	27.44191305542888	22.412821169547538
15-19	22.531895709826607	27.389951211589274	27.4953699943986	22.582783084185518
20-24	22.408689876040373	27.47936956127912	27.471177772641425	22.640762790039084
25-29	22.483394657221332	27.60902221194952	27.44737285965055	22.460210271178603
30-34	22.52120515495474	27.491592692774606	27.32603277131916	22.66116938095149
35-39	22.36042620622734	27.588911308374826	27.540068757717545	22.51059372768029
40-44	22.529072103981992	27.572007154800648	27.382304986912715	22.516615754304645
45-49	22.70089143485404	27.49338347524243	27.27808977650618	22.527635313397344
50-54	22.57397493321006	27.494562059982886	27.343086850867383	22.58837615593967
55-59	22.521134356578106	27.606494293442612	27.39143797749861	22.48093337248067
60-64	22.555454910804457	27.523814283364256	27.317033048265753	22.603697757565534
65-69	22.389607631232078	27.60038064538967	27.411299004449884	22.59871271892836
70-74	22.40941035363789	27.64191014020161	27.408679464514396	22.540000041646106
75-79	22.433273571174233	27.62229898987375	27.26555429923142	22.678873139720597
80-84	22.32187857245485	27.634188952538018	27.39798058042175	22.64595189458538
85-89	21.662629076893282	27.103967416088302	28.743882707912967	22.489520799105442
90-94	22.25994840135316	27.50559695032923	27.450032638200287	22.78442201011732
95-99	22.318754573233647	27.631408772354014	27.32682869608764	22.723007958324693
100-104	22.21553504064257	27.56091453609829	27.52124481486309	22.702305608396045
105-109	22.15368027122037	27.468205532019752	27.558949050656857	22.819165146103025
110-114	22.179096524421478	27.604750969305265	27.524220431016115	22.69193207525714
115-119	22.26006951724955	27.579597948084484	27.3720930863237	22.788239448342264
120-124	22.118682571394636	27.609384393608632	27.558431360362423	22.71350167463431
125-129	22.32302298243528	27.627862659910246	27.429735407220036	22.619378950434438
130-134	22.471527414968566	27.568207058305937	27.28633204566522	22.673933481060278
135-139	22.35790572517954	27.632226128419802	27.43943606940785	22.57043207699281
140-144	22.342294513353302	27.71659941138318	27.471975509800224	22.469130565463296
145-149	22.258750334210266	27.762395866696572	27.475353793438533	22.503500005654633
150-154	22.163987458717642	27.826633328890154	27.571983762111575	22.437395450280636
155-159	22.11934652004658	27.9421896996918	27.63691138140436	22.301552398857254
160-164	22.07994785174638	27.979390182612065	27.555267279766138	22.38539468587542
165-169	21.913732604498875	28.102616840858317	27.73023354091938	22.25341701372342
170-172	21.990644197027063	28.149875079802534	27.697088718548997	22.16239200462141
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.5
15	9.0
16	19.5
17	51.5
18	134.5
19	290.0
20	636.5
21	1303.0
22	2476.0
23	4445.0
24	7382.0
25	11304.0
26	16552.0
27	23291.5
28	31286.0
29	40541.5
30	50966.0
31	62847.5
32	76044.0
33	89632.0
34	104090.5
35	119578.5
36	134914.5
37	149410.0
38	163585.0
39	177215.5
40	189381.5
41	199452.5
42	207391.0
43	212284.5
44	213754.5
45	213015.0
46	209266.5
47	202177.5
48	193115.5
49	182813.0
50	171458.5
51	158915.0
52	145513.0
53	131941.5
54	118929.5
55	106325.0
56	94599.0
57	83882.0
58	74561.0
59	66486.0
60	58619.0
61	51632.0
62	45053.0
63	39037.5
64	33627.5
65	28538.0
66	23758.5
67	19347.0
68	15515.5
69	12319.0
70	9575.5
71	7117.5
72	5210.5
73	3748.5
74	2553.5
75	1707.0
76	1106.5
77	703.0
78	454.0
79	281.0
80	167.5
81	78.5
82	38.5
83	24.5
84	11.0
85	5.5
86	2.0
87	1.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-172	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1680.0
92-93	3637.0
94-95	5543.0
96-97	7301.0
98-99	8997.0
100-101	10518.0
102-103	11981.0
104-105	13495.0
106-107	14633.0
108-109	15380.0
110-111	16628.0
112-113	17582.0
114-115	18568.0
116-117	20404.0
118-119	20075.0
120-121	20451.0
122-123	21589.0
124-125	22662.0
126-127	23330.0
128-129	51940.0
130-131	75672.0
132-133	76670.0
134-135	85236.0
136-137	84566.0
138-139	97504.0
140-141	88084.0
142-143	82012.0
144-145	95056.0
146-147	86590.0
148-149	82465.0
150-151	77757.0
152-153	77126.0
154-155	72910.0
156-157	77703.0
158-159	81203.0
160-161	78383.0
162-163	83483.0
164-165	82330.0
166-167	86977.0
168-169	85172.0
170-171	170174.0
172-173	2648903.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.32479646303442
#Duplication Level	Percentage of total
1	80.85120458359347
2	9.230976246343516
3	2.7105900965302276
4	1.3161129297669663
5	0.8333824746449395
6	0.6794048347369557
7	0.5505265235571167
8	0.4465449236246741
9	0.35537899729183337
>10	2.5781967610986745
>50	0.2730840647001098
>100	0.1607453965802625
>500	0.013852167531228489
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.0823051951432313E-5	0.0	0.0
2	0.0	0.0	0.0	2.0823051951432313E-5	0.0	0.0
3	0.0	0.0	0.0	2.0823051951432313E-5	0.0	0.0
4	0.0	0.0	2.0823051951432313E-5	4.1646103902864625E-5	0.0	0.0
5	0.0	0.0	2.0823051951432313E-5	6.246915585429694E-5	0.0	0.0
6	0.0	0.0	4.1646103902864625E-5	6.246915585429694E-5	0.0	0.0
7	4.1646103902864625E-5	0.0	4.1646103902864625E-5	1.0411525975716157E-4	0.0	0.0
8	4.1646103902864625E-5	0.0	4.1646103902864625E-5	1.0411525975716157E-4	0.0	0.0
9	4.1646103902864625E-5	2.0823051951432313E-5	4.1646103902864625E-5	1.0411525975716157E-4	0.0	0.0
10-14	5.8304545464010476E-5	2.0823051951432313E-5	4.1646103902864625E-5	1.249383117085939E-4	0.0	0.0
15-19	7.079837663486987E-5	3.7481493512578164E-5	4.1646103902864625E-5	1.7074902600174496E-4	4.164610390286463E-6	0.0
20-24	9.162142858630219E-5	4.1646103902864625E-5	4.9975324683437554E-5	2.373827922463284E-4	3.33168831222917E-5	0.0
25-29	1.0411525975716157E-4	4.1646103902864625E-5	7.912759741544279E-5	2.7486428575890654E-4	8.329220780572925E-5	0.0
30-34	2.1655974029489606E-4	4.1646103902864625E-5	1.4159675326973973E-4	2.915227273200524E-4	1.0827987014744803E-4	8.329220780572926E-6
35-39	6.038685065915371E-4	6.246915585429694E-5	1.457613636600262E-4	3.33168831222917E-4	1.4159675326973973E-4	2.0823051951432313E-5
40-44	0.001124444805377345	6.66337662445834E-5	1.4992597405031266E-4	3.748149351257816E-4	2.0406590912403665E-4	3.7481493512578164E-5
45-49	0.001474272078161408	1.1244448053773449E-4	1.915720779531773E-4	4.8309480527322966E-4	2.79028896149193E-4	6.246915585429694E-5
50-54	0.001736642532749455	1.332675324891668E-4	2.2905357146575542E-4	5.247409091760943E-4	3.4566266239377644E-4	6.246915585429694E-5
55-59	0.0019573668834346374	1.5409058444059912E-4	2.665350649783336E-4	5.580577922983861E-4	3.7481493512578167E-4	7.912759741544279E-5
60-64	0.0022197373380226848	1.8740746756289083E-4	3.3733344161320346E-4	5.913746754206776E-4	3.9563798707721397E-4	8.329220780572925E-5
65-69	0.0035982233772075033	2.0823051951432314E-4	4.122964286383598E-4	6.038685065915371E-4	4.164610390286463E-4	8.329220780572925E-5
70-74	0.027403136368084925	2.1239512990460957E-4	4.331194805897921E-4	6.288561689332559E-4	4.3728409098007864E-4	8.329220780572925E-5
75-79	0.037377378252821	2.4571201302690126E-4	4.456133117606515E-4	6.829961040069798E-4	5.538931819080995E-4	8.329220780572925E-5
80-84	0.03747732890218788	3.123457792714847E-4	4.5810714293151083E-4	6.871607143972663E-4	7.45465259861277E-4	8.329220780572925E-5
85-89	0.03754396266843246	3.581564935646358E-4	4.5810714293151083E-4	6.996545455681257E-4	7.496298702515633E-4	8.329220780572925E-5
90-94	0.03765640714897019	3.9147337668692746E-4	4.5810714293151083E-4	7.204775975195581E-4	7.496298702515633E-4	8.329220780572925E-5
95-99	0.03776468701911765	3.9563798707721397E-4	4.706009741023703E-4	7.413006494709905E-4	7.496298702515633E-4	8.329220780572925E-5
100-104	0.03788129611004566	3.9563798707721397E-4	5.330701299566672E-4	7.829467533738549E-4	7.912759741544279E-4	8.329220780572925E-5
105-109	0.03798541136980284	3.9563798707721397E-4	5.997038962012506E-4	8.037698053252873E-4	7.912759741544279E-4	8.329220780572925E-5
110-114	0.03813117273346285	3.9563798707721397E-4	6.246915585429694E-4	8.704035715698706E-4	7.912759741544279E-4	8.329220780572925E-5
115-119	0.03829359253868402	4.164610390286463E-4	6.496792208846882E-4	9.162142858630217E-4	8.204282468864332E-4	8.329220780572925E-5
120-124	0.03876835812317668	4.331194805897921E-4	6.829961040069798E-4	9.162142858630217E-4	8.37086688447579E-4	8.329220780572925E-5
125-129	0.039363897408987644	4.3728409098007864E-4	6.913253247875528E-4	9.412019482047407E-4	8.745681819601573E-4	9.995064936687511E-5
130-134	0.04012185650001978	4.5810714293151083E-4	7.079837663486986E-4	9.786834417173187E-4	8.91226623521303E-4	1.0411525975716157E-4
135-139	0.04069240812348903	4.5810714293151083E-4	7.079837663486986E-4	0.0010203295456201833	9.245435066435947E-4	1.0411525975716157E-4
140-144	0.0410380707858828	4.5810714293151083E-4	7.413006494709905E-4	0.0010494818183521884	9.82848052107605E-4	1.0411525975716157E-4
145-149	0.041404556500228014	4.5810714293151083E-4	7.496298702515633E-4	0.0011202801949870586	0.0010203295456201833	1.0411525975716157E-4
150-154	0.04215835098086986	4.5810714293151083E-4	7.662883118127092E-4	0.0011327740261579179	0.0010494818183521886	1.249383117085939E-4
155-159	0.042724737993948825	4.5810714293151083E-4	7.746175325932821E-4	0.0011452678573287771	0.001061975649523048	1.332675324891668E-4
160-161	0.04309330601348917	4.581071429315109E-4	7.912759741544279E-4	0.0011452678573287771	0.001061975649523048	1.457613636600262E-4
>>END_MODULE
