##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293591
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5611292
Total Bases	923.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.18119944568916	40.0	39.0	41.0	36.0	41.0
2	39.37339386365921	40.0	39.0	41.0	36.0	41.0
3	39.43663152799748	40.0	39.0	41.0	37.0	41.0
4	39.48336532834149	40.0	39.0	41.0	37.0	41.0
5	39.484487173363995	40.0	39.0	41.0	37.0	41.0
6	39.514870372099686	40.0	39.0	41.0	37.0	41.0
7	39.50590398788728	40.0	39.0	41.0	37.0	41.0
8	39.49860745083307	40.0	39.0	41.0	37.0	41.0
9	39.46552148774293	40.0	39.0	41.0	37.0	41.0
10-14	39.41269732532187	40.0	39.0	41.0	36.6	41.0
15-19	39.25181555335207	40.0	38.6	41.0	36.0	41.0
20-24	39.07715916405705	40.0	38.0	41.0	35.6	41.0
25-29	38.86681256295342	40.0	38.0	41.0	35.0	41.0
30-34	38.670495636299094	40.0	37.8	41.0	34.8	41.0
35-39	38.25136079177487	39.6	36.6	40.2	34.0	41.0
40-44	37.87771671836005	39.0	35.6	40.0	33.8	41.0
45-49	37.173668132045165	37.6	35.0	40.0	33.0	41.0
50-54	36.455523255606735	36.4	35.0	39.2	33.0	40.8
55-59	35.784272748593374	35.2	34.0	38.0	33.0	40.0
60-64	35.085148589665266	35.0	33.8	36.6	31.8	39.2
65-69	34.89103817801676	35.0	34.0	36.0	32.0	38.6
70-74	34.69118349214405	35.0	34.0	35.2	32.0	37.0
75-79	34.587129024830645	35.0	34.0	35.0	32.0	37.0
80-84	34.5706779472535	35.0	34.0	35.0	32.0	37.8
85-89	35.186587331402464	35.8	34.4	36.2	31.6	38.6
90-94	38.50235318163614	40.0	37.8	41.0	33.0	41.0
95-99	38.471419803449095	40.0	37.8	41.0	33.0	41.0
100-104	38.34016218076375	40.0	37.0	41.0	33.0	41.0
105-109	38.16858430769244	40.0	36.8	41.0	33.0	41.0
110-114	37.9454293749975	40.0	36.0	41.0	32.2	41.0
115-119	37.694344748800255	39.4	35.4	40.4	32.0	41.0
120-124	37.44359419694964	39.0	35.0	40.0	32.0	41.0
125-129	36.86828137295798	38.0	34.8	39.8	31.8	40.6
130-134	36.65755724997901	37.4	34.2	40.0	31.2	41.0
135-139	36.32452878301423	36.4	34.0	39.8	31.4	41.0
140-144	35.81351733731261	35.4	34.0	38.8	31.0	40.6
145-149	35.225087493912966	35.0	34.0	37.0	31.0	39.6
150-154	34.71377413182948	35.0	34.0	36.0	31.0	38.2
155-159	34.33395489044464	35.0	34.0	35.2	31.0	36.8
160-164	34.07348924001461	35.0	33.6	35.0	31.0	36.0
165-169	33.931103105035184	35.0	33.0	35.0	31.0	35.8
170-174	33.86107426143306	35.0	33.0	35.0	31.0	35.0
175-176	33.44150921563491	34.5	32.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	2.0
28	80.0
29	1337.0
30	8221.0
31	34984.0
32	113253.0
33	239325.0
34	396875.0
35	682795.0
36	1183448.0
37	1627649.0
38	1129144.0
39	192727.0
40	1452.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.175179619952054	28.434681353242713	27.544262533477138	20.8458764933281
2	20.528819387763104	26.63404435199594	30.057765662524783	22.779370597716174
3	20.804869894491322	25.684031413799175	31.282617265328554	22.22848142638095
4	22.589449987632083	26.080054290527034	28.2177616135464	23.11273410829449
5	22.864627255184725	26.767044024798565	27.681610580949982	22.686718139066723
6	22.432587717766246	27.24459892659302	27.882865479108908	22.43994787653182
7	22.88380287463208	27.04765676068898	26.956002289668763	23.112538075010175
8	23.145115242621486	27.359973425015134	26.918934177725912	22.57597715463747
9	22.48111486623758	27.607830781217586	27.4631047537715	22.44794959877333
10-14	22.954218030357357	27.35754974077271	26.89505019521351	22.79318203365642
15-19	22.543064948322062	27.435132586220785	27.410396749982002	22.61140571547515
20-24	22.60902480213113	27.325093757373526	27.453385067111103	22.612496373384243
25-29	22.630552820990246	27.385286668382253	27.2215026414594	22.7626578691681
30-34	22.560483396693666	27.39893058497045	27.33431801446084	22.706268003875042
35-39	22.544387281930792	27.4643700595157	27.372177388024006	22.619065270529497
40-44	22.698430236744052	27.44591441685801	27.209729951676014	22.645925394721928
45-49	22.717598727708342	27.360326284926895	27.24421398850746	22.677860998857305
50-54	22.71703557754613	27.43903899494092	27.297167924962736	22.546757502550214
55-59	22.754253387633366	27.389852461785985	27.128026843015835	22.727867307564818
60-64	22.521319510729437	27.474556661816923	27.320392522791543	22.6837313046621
65-69	22.58398244112051	27.490695547478193	27.309535843082127	22.61578616831917
70-74	22.589047228338856	27.485784022645767	27.198231708490667	22.726937040524714
75-79	22.449471529907907	27.475351487678772	27.304414027999258	22.770762954414064
80-84	22.445436808492587	27.57978020035314	27.29494027400463	22.679842717149633
85-89	22.54757014961973	27.64700535990642	27.268343903685636	22.53708058678821
90-94	21.69695148800685	26.80973586651385	28.688925792545405	22.8043868529339
95-99	22.55751932798097	27.441587181774512	27.135531033417966	22.865362456826553
100-104	22.386856199513836	27.513417537528234	27.233298072059426	22.8664281908985
105-109	22.320314329728042	27.468855856186096	27.518705266041387	22.692124548044475
110-114	22.392710271450962	27.35153461075418	27.333567605423898	22.922187512370964
115-119	22.270267861750412	27.47528758932353	27.39954331011419	22.854901238811873
120-124	22.339293832331443	27.48006296934537	27.375258628333476	22.805384569989712
125-129	22.309306268945793	27.52382927954035	27.34858309783726	22.818281353676596
130-134	22.45454400043343	27.456541884731518	27.269557239432636	22.819356875402423
135-139	22.505350611749655	27.53740687051342	27.325610752033075	22.63163176570385
140-144	22.574233646619533	27.51160320278563	27.22278281946971	22.691380331125124
145-149	22.373207289192106	27.578993461917843	27.393010455873878	22.654788793016174
150-154	22.31261546185014	27.720010748315925	27.499092905618948	22.468280884214987
155-159	22.315037190002396	27.749464801064715	27.386778662243614	22.54871934668927
160-164	22.157120209751856	27.81309513146299	27.565759770342556	22.464024888442594
165-169	22.14096855333631	27.93689999060488	27.536969752454805	22.38516170360401
170-174	22.083594009771804	28.024717972784757	27.566018259213816	22.325669758229626
175-176	21.97509825454619	28.022740318653057	27.677198212477442	22.324963214323315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	6.0
15	11.5
16	26.0
17	69.0
18	163.5
19	355.5
20	818.5
21	1627.0
22	2972.0
23	5291.5
24	8752.0
25	13408.0
26	19668.0
27	27367.0
28	36341.5
29	47136.5
30	59158.0
31	72310.0
32	87152.5
33	102883.5
34	119224.5
35	136481.0
36	153964.0
37	171033.0
38	187685.0
39	203249.5
40	217213.0
41	229186.0
42	238252.5
43	244437.5
44	247256.5
45	246403.0
46	242930.0
47	236163.0
48	225680.0
49	213870.0
50	201041.0
51	186828.0
52	171896.0
53	156567.0
54	141245.0
55	126602.5
56	113201.0
57	101225.5
58	90112.0
59	80151.0
60	71716.5
61	63317.5
62	55383.5
63	48259.5
64	41621.5
65	35304.0
66	29482.5
67	24303.5
68	19439.0
69	15304.0
70	11792.0
71	8843.5
72	6549.0
73	4658.0
74	3212.0
75	2200.0
76	1452.5
77	886.5
78	540.5
79	351.0
80	219.5
81	127.5
82	70.0
83	32.0
84	14.5
85	8.0
86	3.0
87	1.0
88	0.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1830.0
92-93	3803.0
94-95	5551.0
96-97	7673.0
98-99	9483.0
100-101	11058.0
102-103	12496.0
104-105	13967.0
106-107	14844.0
108-109	16063.0
110-111	17717.0
112-113	18138.0
114-115	19396.0
116-117	19939.0
118-119	21627.0
120-121	21993.0
122-123	23136.0
124-125	23494.0
126-127	24779.0
128-129	25370.0
130-131	26058.0
132-133	68841.0
134-135	98209.0
136-137	86144.0
138-139	99606.0
140-141	94068.0
142-143	90991.0
144-145	85833.0
146-147	89223.0
148-149	89316.0
150-151	82048.0
152-153	94176.0
154-155	81651.0
156-157	83684.0
158-159	84027.0
160-161	84607.0
162-163	81905.0
164-165	88220.0
166-167	81598.0
168-169	95495.0
170-171	90270.0
172-173	86376.0
174-175	183937.0
176-177	3252652.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.44979816102965
#Duplication Level	Percentage of total
1	79.67616682246573
2	9.621918784455904
3	2.8052315524454285
4	1.3833676255528276
5	0.9699771612588235
6	0.6970494306995098
7	0.5329976758006197
8	0.4861278561482035
9	0.35749594726172723
>10	2.939611034577177
>50	0.30002950791095295
>100	0.21638094436464717
>500	0.013645657058542485
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.7821207664830133E-5	1.7821207664830133E-5	1.7821207664830133E-5	0.0	0.0	0.0
2	1.7821207664830133E-5	1.7821207664830133E-5	1.7821207664830133E-5	0.0	0.0	0.0
3	1.7821207664830133E-5	3.564241532966027E-5	1.7821207664830133E-5	1.7821207664830133E-5	0.0	0.0
4	1.7821207664830133E-5	3.564241532966027E-5	3.564241532966027E-5	3.564241532966027E-5	0.0	0.0
5	3.564241532966027E-5	3.564241532966027E-5	3.564241532966027E-5	3.564241532966027E-5	0.0	0.0
6	3.564241532966027E-5	3.564241532966027E-5	3.564241532966027E-5	3.564241532966027E-5	0.0	0.0
7	3.564241532966027E-5	3.564241532966027E-5	3.564241532966027E-5	3.564241532966027E-5	0.0	0.0
8	5.346362299449039E-5	3.564241532966027E-5	5.346362299449039E-5	3.564241532966027E-5	0.0	0.0
9	5.346362299449039E-5	3.564241532966027E-5	5.346362299449039E-5	3.564241532966027E-5	0.0	0.0
10-14	5.346362299449039E-5	4.9899381461524373E-5	6.772058912635451E-5	3.564241532966027E-5	2.4949690730762187E-5	0.0
15-19	6.415634759338848E-5	5.346362299449039E-5	7.128483065932053E-5	3.564241532966027E-5	3.9206656862626293E-5	0.0
20-24	7.128483065932053E-5	6.415634759338848E-5	7.128483065932053E-5	7.128483065932053E-5	5.346362299449039E-5	0.0
25-29	7.128483065932053E-5	7.128483065932053E-5	7.841331372525257E-5	1.0692724598898079E-4	8.554179679118461E-5	0.0
30-34	1.8534055971423336E-4	9.267027985711668E-5	8.910603832415065E-5	1.5682662745050515E-4	9.267027985711668E-5	0.0
35-39	5.845356114064283E-4	1.1761997058787888E-4	8.910603832415065E-5	1.9959752584609744E-4	1.1405572905491285E-4	7.128483065932053E-6
40-44	0.001055015493757944	1.782120766483013E-4	1.0336300445601477E-4	2.4949690730762187E-4	1.9246904278016544E-4	2.4949690730762187E-5
45-49	0.0013045124010655656	1.782120766483013E-4	1.1405572905491285E-4	2.8870356417024813E-4	2.530611488405879E-4	3.564241532966027E-5
50-54	0.0016074729313676778	1.782120766483013E-4	1.2474845365381093E-4	3.492956702306706E-4	2.8513932263728213E-4	3.564241532966027E-5
55-59	0.0018712268048071638	1.9603328431313144E-4	1.3900541978567504E-4	3.99195051692195E-4	3.136532549010103E-4	3.564241532966027E-5
60-64	0.0021670588520433442	1.9603328431313144E-4	1.6751935204940323E-4	4.704798823515155E-4	3.279102210328744E-4	4.9899381461524373E-5
65-69	0.0023345782040927474	2.3167569964279173E-4	1.889048012471994E-4	4.847368484833796E-4	3.706811194284667E-4	5.346362299449039E-5
70-74	0.005699222211212676	2.3167569964279173E-4	2.0672600891202955E-4	5.061222976811758E-4	4.312732254888892E-4	5.346362299449039E-5
75-79	0.028973719421480824	2.423684242416898E-4	2.281114581098257E-4	5.27507746878972E-4	4.740441238844815E-4	5.346362299449039E-5
80-84	0.034109791470484874	2.922678057032142E-4	2.3880418270872376E-4	6.130495436701565E-4	5.952283360053264E-4	5.346362299449039E-5
85-89	0.03424523264873758	3.2434597949990835E-4	2.67318114972452E-4	6.308707513349866E-4	7.128483065932052E-4	5.346362299449039E-5
90-94	0.034352159894726564	3.5998839482956867E-4	3.0296053030211224E-4	6.985913404613411E-4	7.128483065932052E-4	5.346362299449039E-5
95-99	0.034459087140715536	3.778096024943988E-4	3.3503870409880645E-4	7.413622388569334E-4	7.128483065932052E-4	5.346362299449039E-5
100-104	0.03459452831896825	3.920665686262629E-4	3.564241532966026E-4	7.734404126536279E-4	7.164125481261713E-4	5.346362299449039E-5
105-109	0.03467650587422647	4.170162593570251E-4	3.742453609614328E-4	8.375967602470162E-4	7.342337557910014E-4	5.346362299449039E-5
110-114	0.034754919187951726	4.2770898395592315E-4	3.8137384402736477E-4	8.589822094448123E-4	7.484907219228655E-4	5.346362299449039E-5
115-119	0.03491887429846816	4.2770898395592315E-4	3.920665686262629E-4	8.839319001755746E-4	7.591834465217635E-4	5.346362299449039E-5
120-124	0.03532519783322628	4.2770898395592315E-4	3.920665686262629E-4	9.516524893019289E-4	7.9839010338439E-4	5.346362299449039E-5
125-129	0.0360166606906217	4.4909443315371936E-4	4.0988777629109304E-4	9.837306630986233E-4	8.090828279832881E-4	5.346362299449039E-5
130-134	0.03658693933589626	4.704798823515155E-4	4.2770898395592315E-4	0.0010086803538293856	8.375967602470162E-4	5.346362299449039E-5
135-139	0.03722137432876421	4.811726069504136E-4	4.2770898395592315E-4	0.0010336300445601477	8.554179679118463E-4	5.346362299449039E-5
140-144	0.03764551907118717	4.811726069504136E-4	4.348374670218552E-4	0.001090657909087604	8.661106925107444E-4	5.346362299449039E-5
145-149	0.037934222635357416	4.811726069504136E-4	4.4553019162075336E-4	0.0011441215320820946	8.732391755766764E-4	5.346362299449039E-5
150-154	0.03842252372537376	4.954295730822777E-4	4.4909443315371936E-4	0.0011833281889447207	8.732391755766764E-4	5.346362299449039E-5
155-159	0.03909972961663731	5.096865392141418E-4	5.45328954543802E-4	0.0012510487780710753	8.910603832415067E-4	5.346362299449039E-5
160-164	0.03967357250344484	5.203792638130398E-4	5.667144037415982E-4	0.0012902554349337014	9.231385570382007E-4	5.346362299449039E-5
165	0.039919505169219494	5.34636229944904E-4	5.880998529393944E-4	0.0013009481595325997	9.267027985711668E-4	5.346362299449039E-5
>>END_MODULE
