##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293592
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5456014
Total Bases	897.4 Mbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.15742261658419	40.0	39.0	41.0	36.0	41.0
2	39.34986750400567	40.0	39.0	41.0	36.0	41.0
3	39.41520146392586	40.0	39.0	41.0	37.0	41.0
4	39.45826110416872	40.0	39.0	41.0	37.0	41.0
5	39.45576111058366	40.0	39.0	41.0	37.0	41.0
6	39.49851008446826	40.0	39.0	41.0	37.0	41.0
7	39.46923413319687	40.0	39.0	41.0	37.0	41.0
8	39.46502135075166	40.0	39.0	41.0	37.0	41.0
9	39.44725435088693	40.0	39.0	41.0	37.0	41.0
10-14	39.38596935418421	40.0	39.0	41.0	36.4	41.0
15-19	39.23964344666271	40.0	38.6	41.0	36.0	41.0
20-24	39.05123340959169	40.0	38.0	41.0	35.6	41.0
25-29	38.85204634738841	40.0	38.0	41.0	35.0	41.0
30-34	38.66621284329549	40.0	37.8	41.0	34.8	41.0
35-39	38.25470550478793	39.6	36.6	40.2	34.0	41.0
40-44	37.88972326683913	39.0	35.6	40.0	33.8	41.0
45-49	37.18962751928422	37.6	35.0	40.0	33.0	41.0
50-54	36.46649528391972	36.4	35.0	39.2	33.0	40.8
55-59	35.78781854298761	35.2	34.0	38.0	33.0	40.0
60-64	35.0885704105598	35.0	33.8	36.6	31.8	39.2
65-69	34.88597866501076	35.0	34.0	36.0	32.0	38.6
70-74	34.685176724253274	35.0	34.0	35.2	32.0	37.0
75-79	34.58226929036472	35.0	34.0	35.0	32.0	37.0
80-84	34.55381742055647	35.0	34.0	35.0	32.0	37.4
85-89	35.156952823068266	35.8	34.4	36.2	31.6	38.6
90-94	38.46827173200459	40.0	37.8	41.0	33.0	41.0
95-99	38.44455939341069	40.0	37.6	41.0	33.0	41.0
100-104	38.306200183279906	40.0	37.0	41.0	33.0	41.0
105-109	38.14312916132995	40.0	36.8	41.0	33.0	41.0
110-114	37.9222785438215	40.0	36.0	41.0	32.4	41.0
115-119	37.675414992175504	39.4	35.4	40.2	32.0	41.0
120-124	37.41680430446732	39.0	35.0	40.0	32.0	41.0
125-129	36.84975485089819	38.0	34.6	39.8	31.8	40.6
130-134	36.64696177772752	37.4	34.2	40.0	31.2	41.0
135-139	36.312952550945475	36.4	34.0	39.8	31.2	41.0
140-144	35.82782165808544	35.4	34.0	38.8	31.0	40.6
145-149	35.23792377083227	35.0	34.0	37.0	31.0	39.6
150-154	34.728692515239004	35.0	34.0	36.0	31.0	38.4
155-159	34.34201586760114	35.0	34.0	35.2	31.0	36.8
160-164	34.07944406047165	35.0	33.4	35.0	31.0	36.0
165-169	33.93731710511445	35.0	33.0	35.0	31.0	35.8
170-174	33.87632022332785	35.0	33.0	35.0	31.0	35.0
175-176	33.4538806932	34.5	32.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	76.0
29	1196.0
30	7515.0
31	32298.0
32	105106.0
33	233352.0
34	395430.0
35	679071.0
36	1159710.0
37	1572677.0
38	1084015.0
39	184192.0
40	1375.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.129962643057734	28.47914246554353	27.588290645881774	20.802604245516964
2	20.424416066381063	26.709810495354304	30.108115558354502	22.75765787991013
3	20.727696079958736	25.735252878749943	31.3516790829349	22.185371958356413
4	22.55921630699628	26.163605885175517	28.280096055471997	22.99708175235621
5	22.763376340309975	26.859406885686145	27.71493988101937	22.66227689298451
6	22.36750125641173	27.28647690420149	27.926614557807223	22.419407281579556
7	22.820267689928947	27.089006736419663	27.05180008702324	23.038925486628152
8	23.06049801191859	27.414665724831348	26.988878694226226	22.535957569023836
9	22.404762891004314	27.646611610600708	27.55799380280183	22.390631695593157
10-14	22.891279237919846	27.43971331451862	26.9511918407834	22.717815606778135
15-19	22.49047014908686	27.494024025598172	27.462924398654405	22.552581426660563
20-24	22.527684129842775	27.39272296588682	27.536325969838053	22.543266934432353
25-29	22.56554693591329	27.44554174531077	27.27418954570131	22.71472177307463
30-34	22.47191447822531	27.48513841789995	27.39281094220066	22.650136161674073
35-39	22.483901250986527	27.545691781582672	27.416161322166694	22.554245645264107
40-44	22.612163385211254	27.513642743585336	27.28457441641462	22.58961945478879
45-49	22.65702397391209	27.43357696662802	27.30039182450778	22.60900723495211
50-54	22.64246755965069	27.512191134406915	27.382818299219906	22.462523006722492
55-59	22.68166100746809	27.462964721131584	27.191719082832265	22.663655188568065
60-64	22.458904980815664	27.523312806748663	27.405244927890582	22.61253728454509
65-69	22.520792651925014	27.546765825747517	27.38143267227687	22.551008850050604
70-74	22.50766951844332	27.55766022594517	27.28212574234597	22.652544513265546
75-79	22.386577453796853	27.544456447509113	27.384361550391915	22.68460454830211
80-84	22.367666212000188	27.65690483932043	27.361165128975106	22.614263819704274
85-89	22.46577813033471	27.72379616327964	27.332979717427413	22.477445988958237
90-94	21.61218424717644	26.880977519829553	28.772686278071337	22.734151954922673
95-99	22.476557158787124	27.52721816371989	27.192835606871512	22.803389070621467
100-104	22.304849952227396	27.582180454733763	27.302090737711733	22.81087885532711
105-109	22.240816893076882	27.553987802273955	27.591948070056528	22.613247234592635
110-114	22.30660043406806	27.429780185632563	27.392309807422393	22.871309572876985
115-119	22.192373396351904	27.541458351543106	27.476562011816736	22.78960624028825
120-124	22.261191617664355	27.561731930796107	27.452009665565985	22.72506678597355
125-129	22.24189963273875	27.589716222289358	27.42488149177976	22.74350265319213
130-134	22.381804729656185	27.526782910482588	27.342664084814533	22.748748275046697
135-139	22.431510903314003	27.608993477724788	27.39576071230434	22.563734906656865
140-144	22.47884015792348	27.60828897961313	27.289247342164952	22.623623520298437
145-149	22.294470247985906	27.662245108383082	27.462897565735755	22.580387077895256
150-154	22.254552291885894	27.789489420874908	27.556839149754964	22.399119137484234
155-159	22.249338641139598	27.823332470223068	27.443806594568485	22.483522294068848
160-164	22.08952457433591	27.909278513103953	27.61228773903113	22.388909173529008
165-169	22.078780077371032	28.018694693791552	27.617017649316995	22.285507579520424
170-174	21.99983512712398	28.135015726087275	27.622111838219165	22.243037308569576
175-176	21.932665137537743	28.093550964337165	27.71411109702121	22.259672801103882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	4.0
15	12.0
16	25.0
17	68.0
18	165.5
19	358.0
20	769.0
21	1603.5
22	3081.0
23	5350.5
24	8742.0
25	13578.0
26	19688.0
27	27324.5
28	36420.0
29	46722.5
30	58455.0
31	71841.0
32	86198.5
33	101640.0
34	117997.0
35	134411.5
36	151240.0
37	168194.5
38	185033.0
39	200433.0
40	213779.0
41	225121.0
42	233591.0
43	238818.0
44	240578.0
45	240147.0
46	236619.5
47	229657.5
48	220251.0
49	208289.5
50	194862.0
51	180309.5
52	165283.0
53	150178.5
54	135381.5
55	121334.5
56	107833.0
57	95815.0
58	85384.5
59	75965.0
60	67515.0
61	59703.0
62	52441.5
63	45772.5
64	39334.0
65	33145.5
66	27533.0
67	22587.0
68	18280.0
69	14343.5
70	10977.5
71	8205.0
72	6015.5
73	4354.0
74	3044.5
75	2027.0
76	1305.5
77	849.0
78	546.0
79	323.0
80	181.0
81	102.0
82	50.5
83	28.5
84	12.0
85	5.0
86	3.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1798.0
92-93	3899.0
94-95	5789.0
96-97	7474.0
98-99	9113.0
100-101	10390.0
102-103	12000.0
104-105	13367.0
106-107	14855.0
108-109	15955.0
110-111	17148.0
112-113	18127.0
114-115	18986.0
116-117	19925.0
118-119	20924.0
120-121	21437.0
122-123	22605.0
124-125	23414.0
126-127	23913.0
128-129	24938.0
130-131	25972.0
132-133	67186.0
134-135	96712.0
136-137	87736.0
138-139	88185.0
140-141	91704.0
142-143	90288.0
144-145	91836.0
146-147	85930.0
148-149	90806.0
150-151	89323.0
152-153	82290.0
154-155	84286.0
156-157	78758.0
158-159	80941.0
160-161	79778.0
162-163	90090.0
164-165	87121.0
166-167	83191.0
168-169	84443.0
170-171	89806.0
172-173	89638.0
174-175	191219.0
176-177	3122718.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.50821831851792
#Duplication Level	Percentage of total
1	79.64004850621497
2	9.852449741047081
3	2.7886462892120227
4	1.3773939588973014
5	0.9433051269434457
6	0.7363835127827174
7	0.5213126990948245
8	0.43528779962921743
9	0.38599815868716997
>10	2.8010637194334964
>50	0.29995996821015536
>100	0.2038515318291812
>500	0.014298988018484969
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.665679743490394E-5	1.832839871745197E-5	0.0	1.832839871745197E-5	0.0	0.0
2	3.665679743490394E-5	1.832839871745197E-5	0.0	3.665679743490394E-5	0.0	0.0
3	3.665679743490394E-5	1.832839871745197E-5	0.0	3.665679743490394E-5	0.0	0.0
4	3.665679743490394E-5	1.832839871745197E-5	0.0	5.498519615235592E-5	0.0	0.0
5	3.665679743490394E-5	1.832839871745197E-5	0.0	7.331359486980788E-5	1.832839871745197E-5	0.0
6	3.665679743490394E-5	1.832839871745197E-5	0.0	1.0997039230471183E-4	1.832839871745197E-5	0.0
7	5.498519615235592E-5	1.832839871745197E-5	0.0	1.0997039230471183E-4	1.832839871745197E-5	0.0
8	5.498519615235592E-5	1.832839871745197E-5	0.0	1.0997039230471183E-4	1.832839871745197E-5	0.0
9	5.498519615235592E-5	1.832839871745197E-5	0.0	1.0997039230471183E-4	1.832839871745197E-5	0.0
10-14	5.498519615235592E-5	1.832839871745197E-5	3.665679743490394E-5	1.0997039230471183E-4	1.832839871745197E-5	0.0
15-19	6.964791512631748E-5	2.1994078460942363E-5	5.498519615235592E-5	1.2096743153518302E-4	2.1994078460942363E-5	0.0
20-24	7.331359486980788E-5	3.665679743490394E-5	5.498519615235592E-5	1.6128990871357735E-4	3.665679743490394E-5	0.0
25-29	8.797631384376944E-5	4.3988156921884734E-5	5.498519615235592E-5	2.2727214409640445E-4	3.665679743490394E-5	0.0
30-34	2.529319023008372E-4	5.498519615235592E-5	5.498519615235592E-5	2.529319023008372E-4	4.398815692188473E-5	0.0
35-39	6.964791512631749E-4	8.431063410027906E-5	6.59822353828271E-5	2.6026326178781804E-4	1.2829879102216378E-4	0.0
40-44	0.001162020478686455	1.4296150999612537E-4	9.164199358725986E-5	3.152484579401739E-4	2.2360646435291408E-4	3.665679743490394E-6
45-49	0.0015725766099573793	1.6862126820055813E-4	9.897335307424064E-5	3.7389933383602015E-4	2.8592301999225074E-4	1.832839871745197E-5
50-54	0.001876828028667082	1.8328398717451971E-4	1.356301505091446E-4	3.848963730664914E-4	3.2624549717064506E-4	2.9325437947923153E-5
55-59	0.00214075697019839	1.9794670614848127E-4	1.4662718973961576E-4	4.3255020973186656E-4	3.738993338360202E-4	5.498519615235591E-5
60-64	0.0024596711078820545	2.2727214409640445E-4	1.4662718973961576E-4	4.948667653712032E-4	4.068904515274338E-4	7.331359486980788E-5
65-69	0.00269427461146544	2.7492598076177954E-4	2.0894374537895247E-4	5.315235628061072E-4	4.252188502448857E-4	7.331359486980788E-5
70-74	0.006121685171628958	2.932543794792315E-4	2.822573402487603E-4	5.86508758958463E-4	4.7653836665375123E-4	7.331359486980788E-5
75-79	0.030241857883795752	3.079170984531931E-4	2.932543794792315E-4	6.158341969063862E-4	5.168608438321456E-4	8.797631384376944E-5
80-84	0.03588333900902747	3.738993338360202E-4	3.079170984531931E-4	6.41493955110819E-4	6.304969158803478E-4	9.164199358725986E-5
85-89	0.03602630051902359	4.3621588947535697E-4	3.189141376836643E-4	7.074761904936461E-4	7.771241056199636E-4	9.164199358725986E-5
90-94	0.03611794251261086	4.6554132742328004E-4	3.4090821614460667E-4	7.881211448504347E-4	7.881211448504347E-4	9.164199358725986E-5
95-99	0.03619858746696764	4.985324451146936E-4	3.4823957563158745E-4	8.284436220288292E-4	7.917868245939251E-4	9.164199358725986E-5
100-104	0.036275566741580945	5.315235628061072E-4	3.9222773255347224E-4	8.724317789507139E-4	8.064495435678868E-4	9.164199358725986E-5
105-109	0.03640019985285962	5.351892425495975E-4	4.142218110144146E-4	9.38414014333541E-4	8.064495435678868E-4	9.164199358725986E-5
110-114	0.03654682704259923	5.535176412670496E-4	4.252188502448857E-4	9.933992104858968E-4	8.064495435678868E-4	9.164199358725986E-5
115-119	0.03673011102977375	5.681803602410111E-4	4.545442881928089E-4	0.0010080619294598585	8.064495435678868E-4	9.164199358725986E-5
120-124	0.03711500740284024	5.938401184454439E-4	4.7653836665375123E-4	0.001033721687664291	8.101152233113771E-4	9.164199358725986E-5
125-129	0.0376648593643638	6.561566740847806E-4	4.8020404639724165E-4	0.001088706883816647	8.321093017723194E-4	9.164199358725986E-5
130-134	0.03830268763973113	6.964791512631749E-4	4.985324451146936E-4	0.0011290293609950415	8.724317789507139E-4	9.164199358725986E-5
135-139	0.03887453367971563	6.964791512631749E-4	5.278578830626168E-4	0.0011656861584299454	8.797631384376947E-4	9.164199358725986E-5
140-144	0.03939872588303476	6.964791512631749E-4	5.315235628061072E-4	0.0012353340735562627	8.870944979246755E-4	9.164199358725986E-5
145-149	0.039765293857383796	7.184732297241174E-4	5.498519615235592E-4	0.0012756565507346571	9.384140143335409E-4	9.164199358725986E-5
150-154	0.040208841106346135	7.404673081850596E-4	5.938401184454438E-4	0.0013123133481695612	0.001004396249716368	9.164199358725986E-5
155-159	0.04078068714633064	7.697927461329828E-4	6.524909943412901E-4	0.0013966239822698402	0.0010557157661252336	9.164199358725986E-5
160-164	0.04137819294451957	7.697927461329828E-4	6.59822353828271E-4	0.0014112867012438018	0.0010813755243296664	9.897335307424066E-5
165	0.04156880829118107	7.697927461329828E-4	6.59822353828271E-4	0.0014112867012438018	0.0010813755243296664	1.0997039230471183E-4
>>END_MODULE
