##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293598
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6012350
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.57722354819663	39.0	39.0	39.0	38.0	39.0
2	40.37080426122897	41.0	40.0	41.0	39.0	41.0
3	40.38914434455745	41.0	40.0	41.0	39.0	41.0
4	40.393827870965595	41.0	40.0	41.0	39.0	41.0
5	40.38866599582526	41.0	40.0	41.0	39.0	41.0
6	40.375725797733	41.0	40.0	41.0	39.0	41.0
7	40.409094114614085	41.0	40.0	41.0	39.0	41.0
8	40.39104526516254	41.0	40.0	41.0	39.0	41.0
9	40.39462256854641	41.0	40.0	41.0	39.0	41.0
10-14	40.341574159854304	41.0	40.0	41.0	39.0	41.0
15-19	40.26566427436859	41.0	40.0	41.0	38.8	41.0
20-24	40.161829268089846	41.0	40.0	41.0	38.0	41.0
25-29	39.996466706030084	41.0	40.0	41.0	38.0	41.0
30-34	39.749674420151855	41.0	39.6	41.0	37.0	41.0
35-39	39.37874801034537	40.2	38.8	41.0	35.4	41.0
40-44	38.959829517576324	40.0	37.6	41.0	35.0	41.0
45-49	38.459598692690875	39.4	36.4	41.0	35.0	41.0
50-54	37.86412643974486	38.4	35.0	40.8	35.0	41.0
55-59	37.0769970477434	36.6	35.0	39.4	35.0	41.0
60-64	36.17290197676449	35.4	34.8	37.8	34.4	40.2
65-69	35.67121288680799	35.0	35.0	36.6	33.8	39.0
70-74	35.36930624464644	35.0	35.0	36.0	34.0	37.0
75-79	35.14033597511788	35.0	35.0	35.0	34.0	36.4
80-84	35.03919844985737	35.0	35.0	35.0	34.0	36.0
85-89	34.9252904771013	35.0	34.8	35.0	33.8	36.0
90-94	39.769413826472125	40.6	39.8	40.6	37.8	40.6
95-99	40.10239406631216	41.0	40.0	41.0	38.0	41.0
100-104	40.00447839622556	41.0	40.0	41.0	38.0	41.0
105-109	39.89532007367991	41.0	40.0	41.0	38.0	41.0
110-114	39.736733509382965	41.0	40.0	41.0	37.0	41.0
115-119	39.522589231459634	40.6	39.0	41.0	36.2	41.0
120-124	39.21970161353714	40.0	38.4	41.0	35.0	41.0
125-129	38.359860728644904	39.4	37.2	40.4	34.4	40.6
130-134	38.43580312735967	39.8	36.8	40.8	34.8	41.0
135-139	38.20956582252428	39.0	35.6	41.0	35.0	41.0
140-144	37.557008789419626	37.6	35.0	40.6	35.0	41.0
145-149	36.77765906109222	36.2	35.0	39.0	34.4	41.0
150-154	36.052630068846	35.0	35.0	37.2	34.0	39.6
155-159	35.465169875949854	35.0	35.0	36.2	34.0	38.0
160-164	35.06630553995866	35.0	35.0	35.4	34.0	36.6
165-169	34.840753071637835	35.0	35.0	35.0	34.0	36.0
170-174	34.72707062671334	35.0	35.0	35.0	34.0	36.0
175-178	34.56841503658373	35.0	34.75	35.0	33.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	17.0
30	172.0
31	1190.0
32	6141.0
33	23885.0
34	74393.0
35	213064.0
36	679639.0
37	1830590.0
38	2482834.0
39	695272.0
40	5152.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.779237735660764	27.261586567648255	28.29637329829434	22.662802398396632
2	22.904305304914054	27.897477691751142	27.83710196512179	21.36111503821301
3	20.949495621512387	26.86475338262077	29.38308648032799	22.802664515538847
4	21.69451212920073	26.038936522324878	29.660748293096713	22.60580305537768
5	22.643242658860512	26.758023069182602	27.764068958061323	22.834665313895567
6	22.733390438015086	26.98392475487954	27.592971134414995	22.689713672690377
7	22.67574243016458	27.2366046554176	27.318103570151436	22.769549344266384
8	22.757723685414188	27.37859572380184	27.11279283474848	22.750887756035493
9	22.88620921935682	27.47256896221943	27.082155895781185	22.55906592264256
10-14	22.81807280015302	27.404113200329324	27.056625113308442	22.72118888620922
15-19	22.793955774364434	27.40702387585553	27.166369223348607	22.632651126431426
20-24	22.75453025855115	27.29305845468078	27.245512985770954	22.706898300997114
25-29	22.778248105981856	27.277227706304526	27.12191738671235	22.82260680100127
30-34	22.781108884213328	27.331110131645698	27.121518208354473	22.766262775786505
35-39	22.75507580230692	27.32347251906492	27.117127246417787	22.804324432210368
40-44	22.78068642045124	27.288717390038837	27.10477600272772	22.825820186782206
45-49	22.90277844769516	27.23200412484303	27.038605536936473	22.826611890525335
50-54	22.87528171180986	27.28091345314228	27.058940347784144	22.784864487263714
55-59	22.841996889735295	27.23617886516919	27.05329862699277	22.86852561810274
60-64	22.82932630335892	27.26659958252597	27.090322419686146	22.813751694428966
65-69	22.850020374728683	27.256071253336884	27.04880454398031	22.845103827954127
70-74	22.80872204711968	27.251537252488628	27.057659650552612	22.88208104983908
75-79	22.831115952996747	27.242216437832127	27.073167729756253	22.85349987941487
80-84	22.819170540637188	27.271389722820526	27.045880562508835	22.863559174033448
85-89	22.781049007459643	27.27784310627292	27.023127396109675	22.917980490157756
90-94	21.895931910886834	27.01632479881846	28.58123240624164	22.506510884053068
95-99	22.538891529226472	27.328222818593524	27.37184191284787	22.76104373933213
100-104	22.673052914627547	27.42226093038821	27.159861880093274	22.74482427489097
105-109	22.568782729475974	27.399249410239474	27.292186576311828	22.739781283972725
110-114	22.5981795203143	27.255005771879905	27.363359077268047	22.783455630537752
115-119	22.620737144827434	27.33695545575326	27.213403080368952	22.828904319050352
120-124	22.591700278659925	27.315178266687678	27.20518876567029	22.887932688982104
125-129	22.5920687175464	27.29730161912775	27.2479380703187	22.862691593007145
130-134	22.717322587110704	27.280703996670013	27.155090595927696	22.846882820291583
135-139	22.78026080628567	27.20985475830328	27.125107710424057	22.88477672498699
140-144	22.77019929766891	27.274334586955458	27.186022422395844	22.76944369297979
145-149	22.74493785446618	27.29025249938241	27.171215570329288	22.793594075822124
150-154	22.66972603503243	27.344315684376557	27.21739359521961	22.768564685371395
155-159	22.687296669262764	27.340223893695786	27.21921653063743	22.75326290640402
160-164	22.619245649466155	27.43920545257034	27.239780115013417	22.70176878295009
165-169	22.564482384689054	27.40169701473627	27.2795347872198	22.754285813354876
170-174	22.53510583268448	27.475194025168964	27.32951741856532	22.660182723581233
175-178	22.507816586859665	27.559032870932356	27.26014008796221	22.673010454245777
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.0
12	3.5
13	6.5
14	14.0
15	28.5
16	57.5
17	123.0
18	245.5
19	501.5
20	1054.0
21	2038.0
22	3630.5
23	6265.0
24	10167.0
25	15315.0
26	22286.5
27	30847.0
28	40683.0
29	51940.0
30	64048.5
31	77109.0
32	91504.0
33	106514.5
34	121438.5
35	136806.5
36	152862.0
37	169316.0
38	185965.0
39	201579.0
40	216138.5
41	230318.0
42	242665.0
43	252592.5
44	260797.0
45	265360.5
46	265004.5
47	260735.5
48	252923.0
49	241834.5
50	228034.5
51	212834.5
52	195780.5
53	177204.5
54	159206.0
55	142543.5
56	126936.0
57	112332.0
58	99530.0
59	88286.5
60	78434.0
61	69477.5
62	60874.0
63	52951.0
64	45769.5
65	39076.0
66	32636.5
67	26929.5
68	21612.0
69	16801.0
70	13029.5
71	9798.0
72	7121.5
73	5061.5
74	3472.0
75	2348.0
76	1515.5
77	914.5
78	566.5
79	339.5
80	191.5
81	107.5
82	65.0
83	32.0
84	12.0
85	7.0
86	4.0
87	2.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	340.0
92-93	658.0
94-95	915.0
96-97	1282.0
98-99	1558.0
100-101	1797.0
102-103	2021.0
104-105	2132.0
106-107	2426.0
108-109	2689.0
110-111	2962.0
112-113	3094.0
114-115	3240.0
116-117	3476.0
118-119	3519.0
120-121	3786.0
122-123	4001.0
124-125	4286.0
126-127	4377.0
128-129	4707.0
130-131	4910.0
132-133	5144.0
134-135	45156.0
136-137	43457.0
138-139	45305.0
140-141	47130.0
142-143	38958.0
144-145	37848.0
146-147	36618.0
148-149	37449.0
150-151	37194.0
152-153	37286.0
154-155	37218.0
156-157	37709.0
158-159	39908.0
160-161	40370.0
162-163	39955.0
164-165	42210.0
166-167	42892.0
168-169	47187.0
170-171	48368.0
172-173	46625.0
174-175	46077.0
176-177	86703.0
178-179	4987407.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.69680885846357
#Duplication Level	Percentage of total
1	88.2469250735536
2	9.622206869528
3	1.5152482581832782
4	0.3737860787938354
5	0.10842379799692789
6	0.06038904228872114
7	0.025195674893241614
8	0.014818941083040812
9	0.01387383891179728
>10	0.019132424767555223
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.6632431578334596E-5	1.6632431578334596E-5	0.0	0.0
3	0.0	1.6632431578334596E-5	1.6632431578334596E-5	1.6632431578334596E-5	1.6632431578334596E-5	0.0
4	0.0	1.6632431578334596E-5	3.326486315666919E-5	1.6632431578334596E-5	3.326486315666919E-5	0.0
5	0.0	1.6632431578334596E-5	3.326486315666919E-5	3.326486315666919E-5	3.326486315666919E-5	0.0
6	1.6632431578334596E-5	1.6632431578334596E-5	3.326486315666919E-5	3.326486315666919E-5	3.326486315666919E-5	0.0
7	1.6632431578334596E-5	1.6632431578334596E-5	3.326486315666919E-5	3.326486315666919E-5	3.326486315666919E-5	0.0
8	3.326486315666919E-5	1.6632431578334596E-5	4.9897294735003785E-5	3.326486315666919E-5	3.326486315666919E-5	0.0
9	3.326486315666919E-5	1.6632431578334596E-5	6.652972631333838E-5	3.326486315666919E-5	4.9897294735003785E-5	0.0
10-14	3.326486315666919E-5	1.6632431578334596E-5	1.1642702104834215E-4	3.326486315666919E-5	7.983567157600605E-5	0.0
15-19	3.326486315666919E-5	1.6632431578334596E-5	1.3305945262667677E-4	4.324432210366995E-5	1.1975350736400907E-4	0.0
20-24	3.991783578800303E-5	2.9938376841002268E-5	1.6632431578334595E-4	4.989729473500378E-5	1.3971242525801062E-4	0.0
25-29	4.989729473500378E-5	3.326486315666919E-5	1.6965080209901286E-4	6.652972631333838E-5	1.5634485683634517E-4	0.0
30-34	7.983567157600605E-5	3.326486315666919E-5	1.9958917894001517E-4	6.652972631333838E-5	1.8960971999301435E-4	0.0
35-39	1.929362063086813E-4	3.326486315666919E-5	2.4283350104368503E-4	6.652972631333838E-5	2.627924189376866E-4	9.979458947000758E-6
40-44	3.0271025472568963E-4	4.324432210366995E-5	2.494864736750189E-4	6.652972631333838E-5	3.359751178823588E-4	1.6632431578334596E-5
45-49	3.9917835788003033E-4	6.320323999767146E-5	2.5281295999068584E-4	6.985621262900531E-5	3.958518715643634E-4	3.326486315666919E-5
50-54	4.52402138930701E-4	8.316215789167298E-5	2.794248505160212E-4	9.314161683867373E-5	4.291167347210325E-4	5.987675368200455E-5
55-59	4.657080841933687E-4	8.316215789167298E-5	2.993837684100227E-4	9.979458947000756E-5	4.4907565261503407E-4	9.646810315434066E-5
60-64	4.8566700208737006E-4	9.646810315434066E-5	3.226691726196911E-4	9.979458947000756E-5	5.621761873477092E-4	1.3638593894234368E-4
65-69	5.355642968223738E-4	1.1642702104834215E-4	3.326486315666919E-4	9.979458947000756E-5	6.619707768177168E-4	1.4969188420501135E-4
70-74	0.0010877610252230825	1.3971242525801062E-4	3.426280905136926E-4	9.979458947000756E-5	7.38479962078056E-4	1.5634485683634517E-4
75-79	0.010628123778555806	1.4969188420501135E-4	3.625870084076941E-4	9.979458947000756E-5	8.116626610227282E-4	1.729772884146798E-4
80-84	0.014723028433141782	1.5634485683634517E-4	3.69239981039028E-4	1.1310053473267525E-4	9.746604904904072E-4	1.8295674736168053E-4
85-89	0.01478955815945512	1.6632431578334595E-4	3.825459263016957E-4	1.6299782946767902E-4	0.0011409848062737533	1.8295674736168053E-4
90-94	0.014802864104717789	1.763037747303467E-4	3.9252538524869646E-4	1.9293620630868132E-4	0.001147637778905087	1.8295674736168053E-4
95-99	0.014829475995243124	1.8628323367734747E-4	4.1581078945836486E-4	2.095686378870159E-4	0.00123079993679676	1.8295674736168053E-4
100-104	0.014836128967874456	1.995891789400151E-4	4.2579024840536556E-4	2.1622161051834972E-4	0.0012540853410064282	1.8295674736168053E-4
105-109	0.01484610842682146	2.0624215157134899E-4	4.4907565261503407E-4	2.1954809683401663E-4	0.001267391286269096	1.8295674736168053E-4
110-114	0.014862740858399792	2.1622161051834972E-4	4.4907565261503407E-4	2.3285404209668435E-4	0.0012806972315317637	1.8295674736168053E-4
115-119	0.014896005721556463	2.1622161051834972E-4	4.956464610343708E-4	2.4283350104368503E-4	0.001307309122057099	1.8295674736168053E-4
120-124	0.014962535447869801	2.262010694653505E-4	5.156053789283724E-4	2.494864736750189E-4	0.0013239415536354336	1.8295674736168053E-4
125-129	0.01503239166049881	2.494864736750189E-4	5.156053789283724E-4	2.494864736750189E-4	0.0014137566841584406	1.8295674736168053E-4
130-134	0.01510224787312781	2.5281295999068584E-4	5.156053789283724E-4	2.494864736750189E-4	0.0015002453283657804	1.8295674736168053E-4
135-139	0.015258592729964156	2.694453915690205E-4	5.488702420850416E-4	2.494864736750189E-4	0.0015335101915224497	1.8295674736168053E-4
140-144	0.0153849992099595	2.827513368316881E-4	5.78808618926044E-4	2.494864736750189E-4	0.0016133458630984555	1.9293620630868132E-4
145-149	0.015464834881535505	2.960572820943558E-4	6.1539996839838E-4	2.6611890525335354E-4	0.0016366312673081241	1.995891789400151E-4
150-154	0.015544670553111511	2.993837684100227E-4	6.1539996839838E-4	3.1601619998835733E-4	0.0017231199115154638	1.995891789400151E-4
155-159	0.015607873793109184	2.993837684100227E-4	6.1539996839838E-4	3.492810631450265E-4	0.001849526391510807	1.995891789400151E-4
160-164	0.015704341896263525	2.993837684100227E-4	6.18726454714047E-4	3.492810631450265E-4	0.0019127296315084783	2.095686378870159E-4
165-167	0.015822986574855643	3.104720561289124E-4	6.542089754144942E-4	3.6591349472336106E-4	0.0019515386385245924	2.1622161051834972E-4
>>END_MODULE
