##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293601
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5399558
Total Bases	925.9 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.37175098406203	39.0	39.0	39.0	37.0	39.0
2	40.14514799174303	41.0	40.0	41.0	38.0	41.0
3	40.17683558543125	41.0	40.0	41.0	38.0	41.0
4	40.189947769798934	41.0	40.0	41.0	38.0	41.0
5	40.19212572584645	41.0	40.0	41.0	38.0	41.0
6	40.18006214582749	41.0	40.0	41.0	38.0	41.0
7	40.20708472804626	41.0	40.0	41.0	38.0	41.0
8	40.18880360207262	41.0	40.0	41.0	38.0	41.0
9	40.1738621939055	41.0	40.0	41.0	38.0	41.0
10-14	40.11646857020519	41.0	40.0	41.0	38.0	41.0
15-19	40.004301315033565	41.0	40.0	41.0	38.0	41.0
20-24	39.84674627071327	41.0	40.0	41.0	38.0	41.0
25-29	39.64147217235188	40.6	39.4	41.0	37.0	41.0
30-34	39.479450317970475	40.4	39.0	41.0	36.2	41.0
35-39	39.40560631073877	40.8	39.0	41.0	35.6	41.0
40-44	38.983388603289384	40.0	37.4	41.0	35.0	41.0
45-49	38.308665450023874	39.2	36.0	41.0	35.0	41.0
50-54	37.52439144092905	37.6	35.0	40.4	34.6	41.0
55-59	36.7289269232778	36.4	35.0	39.0	34.0	41.0
60-64	35.8819787471493	35.2	34.8	37.0	33.6	39.6
65-69	35.480217343715914	35.0	35.0	36.2	33.8	38.8
70-74	35.260006541276155	35.0	35.0	35.8	34.0	37.0
75-79	35.09914618937328	35.0	35.0	35.0	34.0	36.8
80-84	35.03138734689024	35.0	35.0	35.0	33.2	36.0
85-89	34.84624141457505	35.0	34.6	35.0	32.6	36.2
90-94	39.581882278439565	40.6	39.8	40.6	37.8	40.6
95-99	39.88315731008285	41.0	40.0	41.0	38.0	41.0
100-104	39.77049751061578	41.0	40.0	41.0	37.0	41.0
105-109	39.629585312959705	41.0	39.4	41.0	36.6	41.0
110-114	39.45895092072875	40.6	39.0	41.0	36.0	41.0
115-119	39.20648196130629	40.0	38.4	41.0	35.0	41.0
120-124	38.989451435584236	40.0	38.0	41.0	35.0	41.0
125-129	38.5064436324525	39.6	37.2	40.6	34.6	41.0
130-134	38.26145638894626	39.2	36.2	41.0	34.0	41.0
135-139	37.90888978916295	38.8	35.0	41.0	34.6	41.0
140-144	37.26623465471515	37.2	35.0	40.0	34.0	41.0
145-149	36.4957114624539	35.8	35.0	38.6	34.0	40.8
150-154	35.79170303307424	35.0	35.0	36.8	34.0	39.2
155-159	35.25346110080691	35.0	35.0	36.0	34.0	37.4
160-164	34.8998452857894	35.0	35.0	35.0	33.2	36.4
165-169	34.68960763725821	35.0	35.0	35.0	33.0	36.0
170-174	34.58492127055017	35.0	35.0	35.0	33.0	35.8
175-178	34.28777057447573	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	24.0
30	289.0
31	2251.0
32	11202.0
33	41699.0
34	118475.0
35	277509.0
36	688608.0
37	1705936.0
38	2105488.0
39	444345.0
40	3731.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.672810996751956	27.45630290479332	28.44243917742897	22.428446921025756
2	22.662725356408803	27.768717365384354	28.188510985528815	21.38004629267803
3	20.638948595422068	26.929185685198675	29.710376293763304	22.72148942561595
4	21.577580979776492	26.252704388025833	29.783752670125963	22.38596196207171
5	22.365126923351873	26.990098078398265	27.998791752954595	22.645983245295263
6	22.41092696846668	27.163760441132407	27.861428657679017	22.5638839327219
7	22.4375217378904	27.403594886840736	27.58386890186197	22.5750144734069
8	22.464820268621988	27.62842810467079	27.41507730817967	22.491674318527554
9	22.637667749841746	27.591610276248534	27.41000281874924	22.360719155160478
10-14	22.539289327015286	27.597614471406732	27.328225754774742	22.534870446803236
15-19	22.522739824259688	27.587139539940118	27.42964887125946	22.460471764540728
20-24	22.456886285877474	27.446424318434953	27.54896974900538	22.54771964668219
25-29	22.483232886839996	27.464147991372627	27.377740918793727	22.674878202993654
30-34	22.5527533920369	27.504555002465015	27.38023371542634	22.562457890071745
35-39	22.514176160344977	27.48996121534392	27.402131804121744	22.593730820189357
40-44	22.512446389130368	27.5228602044834	27.371084818424023	22.5936085879622
45-49	22.658376852327542	27.418166449920527	27.335067055488615	22.588389642263312
50-54	22.60958767365773	27.49210953933637	27.32964068540425	22.56866210160165
55-59	22.59987947161601	27.444109314132746	27.30959089614372	22.64642031810752
60-64	22.577703582404336	27.467000076672942	27.36204333762134	22.59325300330138
65-69	22.5520348147015	27.463944270994034	27.34983863494012	22.63418227936435
70-74	22.512413053068418	27.476545302411793	27.344301144649247	22.666740499870546
75-79	22.521239701471863	27.4463465342904	27.39554237587595	22.636871388361786
80-84	22.50109360803236	27.468029790586563	27.385567485338612	22.645309116042462
85-89	22.485425658915045	27.4531582029492	27.348131087766813	22.71328505036894
90-94	21.77685104950976	27.171968440708934	28.721478405723605	22.3297021040577
95-99	22.40126724159635	27.550795172150945	27.538711125772974	22.509226460479738
100-104	22.473645753196386	27.631660557286313	27.384955437016735	22.50973825250057
105-109	22.386744539383276	27.607476242387673	27.503640162979536	22.50213905524951
110-114	22.429267724333457	27.447692994812243	27.54163050573521	22.581408775119087
115-119	22.448503645863575	27.522974362292	27.403578931815332	22.624943060029096
120-124	22.417958787297803	27.529734916576192	27.41415939734536	22.638146898780644
125-129	22.4098595130105	27.509422741835248	27.467453144158082	22.613264600996168
130-134	22.496320221754086	27.548276357596706	27.36226792737274	22.593135493276467
135-139	22.57276438894014	27.49594609498801	27.33090785692575	22.600381659146095
140-144	22.580796698186735	27.520216518168265	27.37466884584482	22.524317937800184
145-149	22.527261980199455	27.523967632084993	27.38338385588188	22.565386531833674
150-154	22.473938594554575	27.61618256931881	27.398948807952095	22.510930028174513
155-159	22.469224800130867	27.591421704502896	27.435447146979534	22.503906348386703
160-164	22.416327055827928	27.697542200040164	27.44426308627956	22.441867657852352
165-169	22.3630934410226	27.67598844576808	27.475646124871478	22.485271988337843
170-174	22.36125757959668	27.723707510966854	27.523966176278382	22.39106873315809
175-178	22.323446987489355	27.82275970835833	27.432010221064818	22.421783083087497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.5
12	1.5
13	2.0
14	5.0
15	13.5
16	38.5
17	75.5
18	159.5
19	367.0
20	787.5
21	1509.5
22	2739.5
23	4752.0
24	7641.0
25	11431.5
26	16426.0
27	22920.0
28	30472.0
29	39225.0
30	49318.0
31	60525.5
32	73048.5
33	87016.0
34	102998.0
35	120607.0
36	139441.0
37	159560.5
38	179958.0
39	200336.5
40	219178.5
41	235846.5
42	249223.5
43	258946.5
44	264326.0
45	263989.0
46	259281.0
47	250158.5
48	237635.5
49	222256.5
50	205137.5
51	187237.0
52	168493.5
53	149937.0
54	131876.5
55	115236.5
56	100463.0
57	87169.5
58	75267.5
59	65297.0
60	56550.0
61	48722.0
62	42373.5
63	36650.0
64	31446.0
65	26716.0
66	22376.0
67	18451.5
68	14969.0
69	11771.0
70	9137.5
71	6964.5
72	5093.5
73	3575.5
74	2406.0
75	1667.5
76	1078.0
77	686.5
78	424.5
79	252.5
80	158.5
81	85.0
82	43.5
83	22.5
84	11.5
85	5.5
86	3.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	582.0
92-93	1397.0
94-95	2071.0
96-97	3061.0
98-99	3374.0
100-101	3874.0
102-103	4454.0
104-105	5029.0
106-107	5860.0
108-109	6791.0
110-111	7362.0
112-113	7340.0
114-115	8251.0
116-117	8514.0
118-119	8667.0
120-121	8499.0
122-123	9028.0
124-125	11038.0
126-127	9809.0
128-129	10080.0
130-131	10481.0
132-133	12079.0
134-135	53538.0
136-137	55767.0
138-139	50118.0
140-141	48100.0
142-143	46989.0
144-145	49891.0
146-147	50869.0
148-149	54888.0
150-151	54289.0
152-153	61062.0
154-155	48202.0
156-157	48528.0
158-159	65715.0
160-161	50666.0
162-163	50992.0
164-165	53618.0
166-167	55142.0
168-169	58451.0
170-171	54927.0
172-173	60293.0
174-175	62251.0
176-177	166102.0
178-179	3951519.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.90866681764622
#Duplication Level	Percentage of total
1	90.53312442653763
2	7.839561389645487
3	0.9626354651301343
4	0.3132514401485408
5	0.14719444014310237
6	0.06904689177089042
7	0.046455519755882815
8	0.02876869599605062
9	0.022580564417920487
>10	0.035844938787276154
>50	0.0015362276670915452
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.852003441763196E-5	0.0	0.0
2	0.0	0.0	0.0	3.704006883526392E-5	0.0	0.0
3	0.0	5.556010325289589E-5	0.0	3.704006883526392E-5	0.0	0.0
4	0.0	5.556010325289589E-5	0.0	3.704006883526392E-5	1.852003441763196E-5	0.0
5	0.0	5.556010325289589E-5	0.0	5.556010325289589E-5	1.852003441763196E-5	0.0
6	0.0	9.26001720881598E-5	1.852003441763196E-5	5.556010325289589E-5	5.556010325289589E-5	0.0
7	0.0	9.26001720881598E-5	1.852003441763196E-5	5.556010325289589E-5	5.556010325289589E-5	0.0
8	0.0	9.26001720881598E-5	1.852003441763196E-5	5.556010325289589E-5	7.408013767052785E-5	0.0
9	1.852003441763196E-5	9.26001720881598E-5	1.852003441763196E-5	5.556010325289589E-5	7.408013767052785E-5	0.0
10-14	3.333606195173753E-5	9.26001720881598E-5	5.556010325289589E-5	7.408013767052785E-5	7.408013767052785E-5	0.0
15-19	4.81520894858431E-5	1.2964024092342372E-4	7.037613078700145E-5	1.1482421338931815E-4	8.88961652046334E-5	0.0
20-24	8.148815143758062E-5	1.3334424780695012E-4	9.26001720881598E-5	1.481602753410557E-4	9.26001720881598E-5	0.0
25-29	1.000081858552126E-4	1.481602753410557E-4	1.0741619962226538E-4	1.6297630287516124E-4	1.444562684575293E-4	0.0
30-34	1.1112020650579178E-4	1.481602753410557E-4	1.4075226157400292E-4	2.0742438547747795E-4	2.481684611962683E-4	3.704006883526392E-6
35-39	1.1482421338931815E-4	1.740883235257404E-4	1.777923304092668E-4	2.5928048184684744E-4	3.1854459198326973E-4	2.5928048184684744E-5
40-44	1.9631236482689878E-4	2.000163717104252E-4	1.852003441763196E-4	3.11136578216217E-4	3.852167158867448E-4	5.556010325289589E-5
45-49	3.1484058509974333E-4	2.0372037859395158E-4	2.0372037859395158E-4	3.5928866770206007E-4	5.037449361595893E-4	6.296811701994866E-5
50-54	3.5928866770206E-4	2.0372037859395158E-4	2.1853640612805712E-4	4.259607916055351E-4	6.407931908500657E-4	7.408013767052785E-5
55-59	4.6300086044079905E-4	2.3705644054568907E-4	2.2224041301158355E-4	4.592968535572726E-4	7.667294248899632E-4	7.408013767052785E-5
60-64	5.778250738301173E-4	2.9261654379858493E-4	2.407604474292155E-4	4.926329155090102E-4	8.889616520463342E-4	8.519215832110702E-5
65-69	6.296811701994867E-4	3.333606195173753E-4	2.555764749633211E-4	5.00040929276063E-4	0.0010260099067368107	1.1112020650579178E-4
70-74	0.001085274016873233	3.815127090032184E-4	2.852085300315322E-4	5.00040929276063E-4	0.0011000900444073384	1.2964024092342372E-4
75-79	0.010500859514797322	3.889207227702712E-4	2.963205506821114E-4	5.185609636936949E-4	0.00117046617519434	1.2964024092342372E-4
80-84	0.014367842701198877	3.889207227702712E-4	2.963205506821114E-4	5.407850049948533E-4	0.0013075144298848166	1.2964024092342372E-4
85-89	0.014427106811335296	3.889207227702712E-4	3.0743257133269053E-4	5.667130531795381E-4	0.0015556828910810849	1.2964024092342372E-4
90-94	0.014516002976539932	4.148487709549559E-4	3.481766470514808E-4	6.148651426653811E-4	0.0015742029254987167	1.2964024092342372E-4
95-99	0.01457156307979283	4.259607916055351E-4	3.5928866770206E-4	6.593132252676979E-4	0.0015890189530328221	1.2964024092342372E-4
100-104	0.01460119513486104	4.259607916055351E-4	3.704006883526392E-4	7.222813422876464E-4	0.0016223550149845598	1.2964024092342372E-4
105-109	0.014612307155511617	4.259607916055351E-4	3.9262472965379756E-4	7.852494593075952E-4	0.0016297630287516126	1.2964024092342372E-4
110-114	0.014660459244997462	4.259607916055351E-4	4.0744075718790316E-4	7.963614799581743E-4	0.0016297630287516126	1.2964024092342372E-4
115-119	0.014719723355133885	4.259607916055351E-4	4.0744075718790316E-4	8.482175763275437E-4	0.0016445790562857182	1.2964024092342372E-4
120-124	0.014767875444619726	4.5188883979021985E-4	4.0744075718790316E-4	8.889616520463342E-4	0.001718659193956246	1.2964024092342372E-4
125-129	0.014878995651125517	4.81520894858431E-4	4.0744075718790316E-4	9.14889700231019E-4	0.0018001473453938266	1.2964024092342372E-4
130-134	0.015004931885165416	4.81520894858431E-4	4.4818483290669345E-4	9.371137415321773E-4	0.0018631154624137753	1.555682891081085E-4
135-139	0.01518642822245821	4.852249017419574E-4	4.6300086044079905E-4	9.519297690662829E-4	0.0019223795725501977	1.740883235257404E-4
140-144	0.015442004697421529	5.00040929276063E-4	4.6300086044079905E-4	0.001000081858552126	0.002014979744638357	1.926083579433724E-4
145-149	0.01577166131005538	5.333769912278005E-4	4.6300086044079905E-4	0.0010223058998532844	0.002096467896075938	2.0742438547747795E-4
150-154	0.016034645798785753	5.407850049948533E-4	4.6300086044079905E-4	0.0010482339480379689	0.0021557320062123605	2.2594441989510992E-4
155-159	0.016153174019058598	5.556010325289589E-4	4.6300086044079905E-4	0.0010815700099897066	0.0022112921094652564	2.407604474292155E-4
160-164	0.016260590218680863	5.556010325289589E-4	4.6670486732432545E-4	0.0010926820306402856	0.0022446281714169937	2.444644543127419E-4
165-167	0.01638405711479841	5.556010325289589E-4	4.8152089485843105E-4	0.0010926820306402856	0.0022779642333687314	2.5928048184684744E-4
>>END_MODULE
