##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293604
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5438149
Total Bases	932.1 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.36806953983791	39.0	39.0	39.0	37.0	39.0
2	40.13964383837221	41.0	40.0	41.0	38.0	41.0
3	40.17645526078818	41.0	40.0	41.0	38.0	41.0
4	40.18891336004218	41.0	40.0	41.0	38.0	41.0
5	40.18592631426612	41.0	40.0	41.0	38.0	41.0
6	40.17837411222091	41.0	40.0	41.0	38.0	41.0
7	40.20511059921308	41.0	40.0	41.0	38.0	41.0
8	40.192116839755585	41.0	40.0	41.0	38.0	41.0
9	40.17689070306827	41.0	40.0	41.0	38.0	41.0
10-14	40.1153988976764	41.0	40.0	41.0	38.0	41.0
15-19	40.00731634973591	41.0	40.0	41.0	38.0	41.0
20-24	39.85406733063033	41.0	40.0	41.0	38.0	41.0
25-29	39.641030431494244	40.6	39.4	41.0	37.0	41.0
30-34	39.46969117617042	40.2	39.0	41.0	36.0	41.0
35-39	39.395336317559526	40.8	39.0	41.0	35.4	41.0
40-44	38.978578924556864	40.0	37.4	41.0	35.0	41.0
45-49	38.310460084856075	39.2	36.0	41.0	35.0	41.0
50-54	37.53180712775615	37.6	35.0	40.4	34.8	41.0
55-59	36.746492676092544	36.4	35.0	39.0	34.0	41.0
60-64	35.91120121938549	35.2	34.8	37.2	33.6	39.6
65-69	35.513809275913545	35.0	35.0	36.4	33.8	39.0
70-74	35.28201559023116	35.0	35.0	36.0	34.0	37.2
75-79	35.111863871328275	35.0	35.0	35.0	34.0	37.0
80-84	35.05052619926376	35.0	35.0	35.0	33.2	36.2
85-89	34.86982456714592	35.0	34.6	35.0	32.6	36.4
90-94	39.57930974664639	40.6	39.8	40.6	37.8	40.6
95-99	39.87109910270651	41.0	40.0	41.0	37.8	41.0
100-104	39.7572829669743	41.0	40.0	41.0	37.0	41.0
105-109	39.615890535419645	41.0	39.4	41.0	36.4	41.0
110-114	39.44612856995758	40.6	39.0	41.0	35.8	41.0
115-119	39.197022159486465	40.0	38.2	41.0	35.0	41.0
120-124	38.97486220338181	40.0	38.0	41.0	35.0	41.0
125-129	38.49657807118379	39.6	37.2	40.6	34.6	41.0
130-134	38.25143869543551	39.2	36.2	41.0	34.0	41.0
135-139	37.908292440890136	38.8	35.0	41.0	34.8	41.0
140-144	37.26723353664825	37.2	35.0	40.0	34.0	41.0
145-149	36.49829874085345	35.8	35.0	38.6	34.0	40.8
150-154	35.79677223175362	35.0	35.0	36.8	34.0	39.2
155-159	35.257150974425485	35.0	35.0	36.0	34.0	37.4
160-164	34.90116983267481	35.0	35.0	35.0	33.2	36.4
165-169	34.69845385123022	35.0	35.0	35.0	33.0	36.0
170-174	34.586958767371	35.0	35.0	35.0	33.0	35.8
175-178	34.29321747241695	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	25.0
30	298.0
31	2102.0
32	10851.0
33	41934.0
34	118384.0
35	279792.0
36	693130.0
37	1716697.0
38	2119107.0
39	451655.0
40	4172.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.67678745102424	27.475414888411482	28.42283284257199	22.424964817992297
2	22.62100578707939	27.78436192167592	28.20341995042799	21.3912123408167
3	20.609016045717027	26.919582379960534	29.755657669548956	22.71574390477348
4	21.5642123818233	26.298727747253707	29.803946158886045	22.333113712036944
5	22.366415484386323	26.997531696906428	28.031799055156455	22.604253763550798
6	22.393501906623005	27.22288411001611	27.851057409423685	22.5325565739372
7	22.44307759864616	27.421591427524326	27.5960441687052	22.539286805124316
8	22.4840841985021	27.606010795217266	27.406898928293433	22.503006077987198
9	22.627368246070493	27.60481553558021	27.360486077156033	22.407330141193263
10-14	22.542199560916774	27.636208570232263	27.30789465312554	22.513697215725426
15-19	22.506525657903083	27.598953246775693	27.450803573053996	22.443717522267228
20-24	22.46301452939226	27.453764139231936	27.527005972068807	22.556215359307
25-29	22.469435831934724	27.478912401995604	27.393239868933346	22.658411897136325
30-34	22.542412868790464	27.496609600067963	27.39329871248471	22.567678818656862
35-39	22.51044243179067	27.484524605706834	27.417446634875215	22.58758632762729
40-44	22.504841261245325	27.514089812544672	27.39924558889431	22.581823337315694
45-49	22.6427705456397	27.42297425098135	27.33280018623984	22.601455017139106
50-54	22.604172853667674	27.495131155839974	27.331211410353045	22.56948458013931
55-59	22.587534839519844	27.442984736166665	27.324164895077352	22.645315529236143
60-64	22.562125458496997	27.480026751749538	27.38513049201116	22.5727172977423
65-69	22.526992180611455	27.46572776876838	27.386546414965828	22.62073363565434
70-74	22.513785481052466	27.489173246264492	27.36965096028079	22.627390312402255
75-79	22.523237226490117	27.472954492420122	27.393773138617572	22.61003514247219
80-84	22.499758649496364	27.482711488780463	27.374428321107054	22.64310154061612
85-89	22.485790661491624	27.47207183914968	27.350975488167023	22.691162011191675
90-94	21.782370835784292	27.168319341652914	28.720813964745616	22.32849585781718
95-99	22.387840977618602	27.55639091930621	27.54857764090099	22.507190462174194
100-104	22.488373868506045	27.600511774765994	27.406139895675548	22.504974461052413
105-109	22.381193928225095	27.598146042910383	27.524108573451716	22.496551455412803
110-114	22.425692304725338	27.45314046068101	27.555485996656227	22.565681237937422
115-119	22.432322613720522	27.53502174120175	27.429874324635424	22.6027813204423
120-124	22.416875020239544	27.526495387328236	27.43697396347541	22.61965562895681
125-129	22.391432692710904	27.52657753654632	27.485864467527165	22.596125303215608
130-134	22.488884728858093	27.537837551275935	27.39446380231171	22.578813917554257
135-139	22.558778224055747	27.47609269124693	27.355806111249066	22.609322973448258
140-144	22.558394686965244	27.52642088614497	27.404321640467394	22.510862786422393
145-149	22.542590066024037	27.525756779598776	27.393380923948023	22.53827223042917
150-154	22.46593586300373	27.611269477303296	27.41062511197741	22.512169547715565
155-159	22.47091666265724	27.57670681442753	27.459246547880618	22.493129975034616
160-164	22.39796838257351	27.699028309668925	27.467058384556747	22.43594492320082
165-169	22.372273229115343	27.685430023775297	27.48099072381025	22.461306023299105
170-174	22.372946394741113	27.7203812364222	27.52943574914572	22.37723661969097
175-178	22.33989481805419	27.777424350651863	27.457760189237963	22.42492064205598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.5
12	1.5
13	2.0
14	6.5
15	17.0
16	32.0
17	91.5
18	188.5
19	368.0
20	755.5
21	1535.0
22	2818.5
23	4781.5
24	7697.0
25	11587.5
26	16690.0
27	23200.5
28	30767.5
29	39731.0
30	49831.5
31	60922.5
32	73762.5
33	88096.0
34	103798.5
35	121569.0
36	141329.0
37	161373.5
38	181555.0
39	201673.0
40	220580.5
41	237351.0
42	251600.5
43	261672.5
44	265917.0
45	265144.0
46	260761.0
47	252177.5
48	239230.0
49	224052.5
50	206318.0
51	187600.5
52	169473.0
53	150962.5
54	132881.0
55	116356.0
56	101253.5
57	87774.0
58	75972.5
59	65740.5
60	56780.5
61	48895.5
62	42336.5
63	36493.5
64	31218.5
65	26618.5
66	22439.5
67	18459.0
68	14840.5
69	11777.0
70	9123.0
71	6951.5
72	5143.0
73	3624.0
74	2508.0
75	1692.5
76	1093.5
77	679.0
78	428.0
79	245.5
80	137.5
81	80.5
82	44.5
83	25.0
84	10.5
85	4.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	600.0
92-93	1460.0
94-95	2714.0
96-97	3262.0
98-99	3340.0
100-101	3933.0
102-103	4524.0
104-105	5147.0
106-107	6134.0
108-109	6695.0
110-111	7440.0
112-113	8176.0
114-115	8745.0
116-117	8969.0
118-119	8700.0
120-121	10010.0
122-123	9657.0
124-125	10722.0
126-127	10748.0
128-129	10638.0
130-131	11482.0
132-133	12510.0
134-135	56387.0
136-137	52322.0
138-139	51818.0
140-141	51595.0
142-143	48656.0
144-145	52417.0
146-147	53494.0
148-149	59013.0
150-151	54928.0
152-153	49686.0
154-155	51632.0
156-157	48939.0
158-159	50463.0
160-161	53240.0
162-163	50808.0
164-165	52318.0
166-167	54541.0
168-169	66473.0
170-171	57763.0
172-173	61635.0
174-175	61732.0
176-177	169642.0
178-179	3973041.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.91901813432992
#Duplication Level	Percentage of total
1	90.55159931078967
2	7.796822906404084
3	1.024587806048301
4	0.30087148776397576
5	0.12954021673283192
6	0.0732084705267174
7	0.0401054204927146
8	0.029820930605155716
9	0.01612076346196736
>10	0.035754502631437916
>50	0.0015681845431210202
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.8388609800871583E-5	0.0	1.8388609800871583E-5	0.0	0.0
2	1.8388609800871583E-5	3.6777219601743166E-5	0.0	5.516582940261475E-5	1.8388609800871583E-5	0.0
3	3.6777219601743166E-5	5.516582940261475E-5	0.0	5.516582940261475E-5	3.6777219601743166E-5	0.0
4	3.6777219601743166E-5	7.355443920348633E-5	0.0	5.516582940261475E-5	5.516582940261475E-5	0.0
5	3.6777219601743166E-5	7.355443920348633E-5	1.8388609800871583E-5	5.516582940261475E-5	5.516582940261475E-5	0.0
6	3.6777219601743166E-5	7.355443920348633E-5	1.8388609800871583E-5	5.516582940261475E-5	5.516582940261475E-5	0.0
7	3.6777219601743166E-5	7.355443920348633E-5	1.8388609800871583E-5	7.355443920348633E-5	5.516582940261475E-5	0.0
8	3.6777219601743166E-5	7.355443920348633E-5	1.8388609800871583E-5	7.355443920348633E-5	7.355443920348633E-5	0.0
9	3.6777219601743166E-5	7.355443920348633E-5	1.8388609800871583E-5	1.103316588052295E-4	7.355443920348633E-5	0.0
10-14	5.884355136278906E-5	7.355443920348633E-5	2.5744053721220217E-5	1.2504254664592678E-4	8.82653270441836E-5	0.0
15-19	1.287202686061011E-4	8.82653270441836E-5	3.6777219601743166E-5	1.765306540883672E-4	1.3607571252644972E-4	0.0
20-24	1.6549748820784426E-4	1.103316588052295E-4	3.6777219601743166E-5	2.1330787369011037E-4	1.8388609800871583E-4	0.0
25-29	1.6549748820784426E-4	1.103316588052295E-4	5.516582940261475E-5	2.647959811325508E-4	2.0963015172993605E-4	0.0
30-34	1.765306540883672E-4	1.103316588052295E-4	6.252127332296338E-5	2.942177568139453E-4	2.6847370309272513E-4	0.0
35-39	2.537628152520279E-4	1.1400938076540382E-4	1.1400938076540382E-4	3.6409447405725734E-4	3.7512763993778023E-4	2.20663317610459E-5
40-44	3.420281422962114E-4	1.6549748820784423E-4	1.5814204428749563E-4	4.5235980110144093E-4	4.082271375793491E-4	5.516582940261475E-5
45-49	4.5235980110144093E-4	2.0227470780958745E-4	1.6549748820784426E-4	4.7810385482266124E-4	5.038479085438814E-4	8.82653270441836E-5
50-54	5.001701865837071E-4	2.169855956502847E-4	1.985969858494131E-4	5.075256305040557E-4	5.774023477473677E-4	1.3607571252644972E-4
55-59	5.369474061854504E-4	2.20663317610459E-4	2.3905192741133062E-4	5.259142403049273E-4	6.325681771499825E-4	1.6549748820784426E-4
60-64	5.737246257871934E-4	2.20663317610459E-4	2.427296493715049E-4	5.626914599066704E-4	6.767008406720743E-4	1.985969858494131E-4
65-69	6.546345089110284E-4	2.3905192741133062E-4	2.7215142505289944E-4	6.031464014685879E-4	7.870324994773038E-4	2.0227470780958745E-4
70-74	0.001114349753932818	2.427296493715049E-4	3.126063666148169E-4	6.840562845924229E-4	9.378190998444507E-4	2.133078736901104E-4
75-79	0.00920901578827649	2.574405372122022E-4	3.162840885749912E-4	7.428998359552119E-4	0.001044473036689506	2.3537420545115625E-4
80-84	0.012581486825756336	3.0157320073429395E-4	3.457058642563858E-4	7.723216116366065E-4	0.0011695155833354326	2.7215142505289944E-4
85-89	0.012636652655158953	3.60416752097083E-4	3.493835862165601E-4	8.054211092781753E-4	0.00137179029114502	2.7582914701307376E-4
90-94	0.01268446304064122	4.045494156191748E-4	3.493835862165601E-4	8.495537728002671E-4	0.0013791457350653688	2.7582914701307376E-4
95-99	0.01272124026024296	4.2661574738022073E-4	3.640944740572573E-4	9.083973241630561E-4	0.001393856622906066	2.7582914701307376E-4
100-104	0.012739628870043835	4.486820791412666E-4	3.7144991797760597E-4	9.672408755258454E-4	0.0014232783985874604	2.7582914701307376E-4
105-109	0.01281318330924732	4.597152450217895E-4	4.119048595395235E-4	0.0010702170904107263	0.0014343115644679836	2.7582914701307376E-4
110-114	0.012886737748450805	4.744261328624868E-4	4.302934693403951E-4	0.0011106720319726438	0.0014343115644679836	2.7582914701307376E-4
115-119	0.012912481802172027	4.7810385482266124E-4	4.450043571810923E-4	0.0011217051978531665	0.0014894773938705981	2.7582914701307376E-4
120-124	0.012956614465694117	4.7810385482266124E-4	4.597152450217895E-4	0.0011364160856938638	0.0016145199405165251	2.7582914701307376E-4
125-129	0.01313682284174266	4.928147426633584E-4	4.6339296698196393E-4	0.0011584824174549098	0.0016954298236403602	2.7582914701307376E-4
130-134	0.01333541982759207	5.001701865837071E-4	5.075256305040557E-4	0.0011695155833354328	0.0017579510969633234	2.942177568139453E-4
135-139	0.013420007432676082	5.443028501057989E-4	5.443028501057989E-4	0.0011879041931363043	0.00188299364360925	2.942177568139453E-4
140-144	0.013581827198923752	5.516582940261475E-4	5.70046903827019E-4	0.0012467477444990933	0.001974936692613608	2.942177568139453E-4
145-149	0.013916499897299614	5.516582940261475E-4	5.81080069707542E-4	0.0012872026860610108	0.0020448134098569196	3.0157320073429395E-4
150-154	0.014218073098033907	5.70046903827019E-4	5.994686795084135E-4	0.0012945581299813596	0.0021551450686621496	3.126063666148169E-4
155-159	0.014376215142321406	5.70046903827019E-4	6.252127332296338E-4	0.0013055912958618823	0.002221344063945287	3.3467269837586283E-4
160-164	0.014479191357206284	5.957909575482393E-4	6.325681771499825E-4	0.0013055912958618823	0.002254443561586856	3.493835862165601E-4
165-167	0.014667981084495234	6.252127332296338E-4	6.436013430305054E-4	0.0013055912958618826	0.0022924466885086573	3.861608058183033E-4
>>END_MODULE
