##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293610
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10794617
Total Bases	1.8 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.351991552826746	39.0	39.0	39.0	37.0	39.0
2	40.12751763216796	41.0	40.0	41.0	38.0	41.0
3	40.16414959419125	41.0	40.0	41.0	38.0	41.0
4	40.17648833673302	41.0	40.0	41.0	38.0	41.0
5	40.17624256608641	41.0	40.0	41.0	38.0	41.0
6	40.161058053287114	41.0	40.0	41.0	38.0	41.0
7	40.18181163815261	41.0	40.0	41.0	38.0	41.0
8	40.165182979627716	41.0	40.0	41.0	38.0	41.0
9	40.14437483052896	41.0	40.0	41.0	38.0	41.0
10-14	40.07444141834768	41.0	40.0	41.0	38.0	41.0
15-19	39.94624146461148	41.0	40.0	41.0	38.0	41.0
20-24	39.76361399390085	41.0	39.8	41.0	37.6	41.0
25-29	39.50335881300837	40.2	39.0	41.0	36.6	41.0
30-34	39.30750336023964	40.0	38.8	41.0	35.4	41.0
35-39	39.20452444028352	40.0	38.2	41.0	35.0	41.0
40-44	38.737000840326246	40.0	36.8	41.0	35.0	41.0
45-49	38.07109612133529	39.0	35.4	41.0	35.0	41.0
50-54	37.36039303664039	37.2	35.0	40.2	34.2	41.0
55-59	36.66177299296491	36.2	35.0	39.0	34.0	41.0
60-64	35.91751577661348	35.0	34.8	37.4	33.6	40.0
65-69	35.55533723892196	35.0	35.0	36.4	33.6	39.0
70-74	35.32689750826732	35.0	35.0	36.0	34.0	37.8
75-79	35.13788237229723	35.0	35.0	35.2	33.6	37.0
80-84	35.05855327706393	35.0	35.0	35.0	33.0	37.0
85-89	34.86408192157258	35.0	34.6	35.0	32.6	37.2
90-94	39.638534395416926	40.6	39.8	40.6	37.8	40.6
95-99	39.90175274061433	41.0	40.0	41.0	38.0	41.0
100-104	39.77539526496912	41.0	40.0	41.0	37.0	41.0
105-109	39.620579281802726	41.0	39.4	41.0	36.4	41.0
110-114	39.427654554170026	40.4	39.0	41.0	35.6	41.0
115-119	39.14620788190351	40.0	38.2	41.0	35.0	41.0
120-124	38.88710050226057	40.0	38.0	41.0	35.0	41.0
125-129	38.39295062285865	39.6	36.6	40.6	34.6	41.0
130-134	38.119338650236436	39.0	35.6	41.0	34.0	41.0
135-139	37.761214312580556	38.6	35.0	41.0	34.0	41.0
140-144	37.17439490577148	36.8	35.0	40.0	34.0	41.0
145-149	36.49343298424658	35.6	35.0	38.8	34.0	41.0
150-154	35.859595604866584	35.0	35.0	37.0	34.0	39.4
155-159	35.33491075841199	35.0	35.0	36.0	33.8	38.0
160-164	34.96624507205429	35.0	35.0	35.2	33.0	36.6
165-169	34.726980421217476	35.0	35.0	35.0	33.0	36.0
170-174	34.597144972225394	35.0	35.0	35.0	33.0	36.0
175-178	34.26988540408575	34.75	34.5	35.0	32.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	38.0
30	617.0
31	4733.0
32	23968.0
33	88287.0
34	249626.0
35	609587.0
36	1611447.0
37	3432589.0
38	3648970.0
39	1111619.0
40	13134.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.425882919236507	26.96663531462024	29.20399121154553	22.40349055459772
2	22.93628389038722	27.833919443367005	28.041939792768932	21.187856873476846
3	20.068771314443115	26.56751045451636	30.482369128983457	22.881349102057072
4	21.328575159266883	25.31974038541617	30.69141776868971	22.660266686627235
5	22.531906412242325	26.28070083449927	28.09535530533413	23.092037447924277
6	22.482807866179968	26.844648587346825	27.93340421434128	22.739139332131934
7	22.624128303950013	26.984431221598694	27.39856356181975	22.992876912631548
8	22.878375397663483	27.133977981803337	26.972314070985565	23.01533254954761
9	22.85593828850065	27.35152159636604	27.185216483363885	22.607323631769425
10-14	22.853670491505166	27.22633142055897	27.00649407014626	22.913504017789606
15-19	22.809329872472546	27.220804591770136	27.15794363060774	22.811921905149575
20-24	22.7658285606613	27.049243155176327	27.242228232831234	22.94270005133114
25-29	22.843457993924194	26.99225363901285	27.041517082078965	23.122771284983987
30-34	22.865695003352133	27.086018892564695	27.058566320602207	22.989719783480968
35-39	22.88445991182457	27.065834758194757	27.05127935525642	22.998425974724253
40-44	22.919453279352105	27.0168807286076	26.990997457343784	23.072668534696504
45-49	23.01443765906655	26.978252215896127	26.992550083064547	23.014760041972774
50-54	23.022877050663308	27.03888799389548	26.943399659293142	22.994835296148068
55-59	22.968081220482397	26.956407994836685	26.961456807592153	23.114053977088766
60-64	22.943852477582112	27.020719679077082	27.026213157910096	23.00921468543071
65-69	22.923527532287622	27.02219819378492	26.998666094406126	23.055608179521332
70-74	22.90178521387095	27.0113001693344	26.984822157191868	23.102092459602783
75-79	22.859138031483656	27.01091664484252	27.07653824123635	23.053407082437477
80-84	22.877702840221197	27.03381138951016	27.003512954651377	23.084972815617267
85-89	22.841981331991676	26.9669929002576	26.999462787795064	23.191562979955656
90-94	21.726491814155263	26.70309308415999	29.084296819988616	22.486118281696132
95-99	22.63775029617238	27.189303505083025	27.30736999443821	22.865576204306382
100-104	22.793564818248115	27.2810533023836	27.087980981937093	22.83740089743119
105-109	22.707707242999938	27.211058601179978	27.185133242600163	22.896100913219918
110-114	22.730288229900843	27.052252638030726	27.27820824487003	22.939250887198405
115-119	22.820213665033805	27.12729186117398	27.0316745159618	23.020819957830415
120-124	22.77708827356501	27.108494237908467	27.055456160417513	23.05896132810901
125-129	22.749290673932684	27.11166443065193	27.137834182625486	23.001210712789895
130-134	22.859339470999256	27.14750335382074	26.9881448202592	23.0050123549208
135-139	22.941287473967336	27.06023734275376	26.95257134990902	23.045903833369884
140-144	22.929195829716694	27.14157748085995	27.020089669037766	22.90913702038559
145-149	22.893740463920413	27.165818619313086	27.02451109620511	22.91592982056139
150-154	22.834332903520735	27.221548738833988	27.018422173721735	22.925696183923538
155-159	22.783104409560977	27.253913984107392	27.13021588262876	22.83276572370287
160-164	22.741093546030903	27.360186056348844	27.10606705138789	22.79265334623236
165-169	22.666209836476366	27.327414806190163	27.16261473102421	22.84376062630926
170-174	22.641377161330787	27.44200867358232	27.23124625613172	22.685367908955172
175-178	22.608028764832792	27.506842881161543	27.14411432091791	22.741014033087755
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	2.5
12	12.0
13	35.0
14	70.0
15	138.5
16	287.0
17	573.0
18	1133.5
19	2233.0
20	4131.0
21	7274.0
22	11938.0
23	18272.0
24	26654.5
25	37044.5
26	49683.5
27	64671.0
28	81637.5
29	100617.0
30	121695.0
31	143621.0
32	166649.0
33	191530.0
34	216935.5
35	242674.5
36	268756.5
37	295120.5
38	321113.5
39	347495.0
40	372307.0
41	394378.0
42	414463.5
43	429520.0
44	439663.5
45	445092.5
46	444373.0
47	437356.0
48	425155.5
49	408687.0
50	388383.0
51	366125.5
52	342483.0
53	318039.0
54	293862.0
55	270490.0
56	247556.0
57	225132.5
58	204520.0
59	184230.5
60	164682.0
61	146724.0
62	129147.5
63	112055.5
64	96204.0
65	81355.5
66	67279.0
67	54965.5
68	44267.5
69	34712.5
70	26525.5
71	19752.5
72	14361.5
73	10184.5
74	7018.0
75	4722.5
76	3057.0
77	1910.0
78	1153.5
79	693.5
80	403.5
81	215.5
82	127.5
83	68.0
84	30.0
85	10.0
86	4.5
87	4.5
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1332.0
92-93	2741.0
94-95	4125.0
96-97	5359.0
98-99	6683.0
100-101	8009.0
102-103	9442.0
104-105	10668.0
106-107	11982.0
108-109	13210.0
110-111	14103.0
112-113	14821.0
114-115	17252.0
116-117	17581.0
118-119	17292.0
120-121	17978.0
122-123	18799.0
124-125	19673.0
126-127	20423.0
128-129	21306.0
130-131	22223.0
132-133	24600.0
134-135	108207.0
136-137	104113.0
138-139	91051.0
140-141	91891.0
142-143	102370.0
144-145	116618.0
146-147	113722.0
148-149	113075.0
150-151	106521.0
152-153	105173.0
154-155	98169.0
156-157	102366.0
158-159	101957.0
160-161	107232.0
162-163	107383.0
164-165	107858.0
166-167	119016.0
168-169	113380.0
170-171	117167.0
172-173	126049.0
174-175	132373.0
176-177	372414.0
178-179	7836910.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.29392145739752
#Duplication Level	Percentage of total
1	87.54301319995916
2	10.247473637912405
3	1.405767675738452
4	0.354595197589743
5	0.17099561193681564
6	0.10736746886039625
7	0.0534184576501952
8	0.03508878708535425
9	0.0176253054598275
>10	0.05017150119491818
>50	0.0027328179468052503
>100	0.0
>500	0.0
>1k	0.011750338665944248
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0380528554185851E-4	9.263876615539023E-6	1.8527753231078046E-5	1.8527753231078046E-5	9.263876615539023E-6	0.0
2	7.040546227809658E-4	9.263876615539023E-6	2.7791629846617067E-5	1.8527753231078046E-5	1.8527753231078046E-5	0.0
3	7.133184993965047E-4	1.8527753231078046E-5	3.705550646215609E-5	2.7791629846617067E-5	1.8527753231078046E-5	0.0
4	7.133184993965047E-4	1.8527753231078046E-5	4.6319383077695116E-5	2.7791629846617067E-5	1.8527753231078046E-5	0.0
5	7.225823760120438E-4	2.7791629846617067E-5	4.6319383077695116E-5	2.7791629846617067E-5	1.8527753231078046E-5	0.0
6	7.318462526275828E-4	2.7791629846617067E-5	7.411101292431218E-5	4.6319383077695116E-5	1.8527753231078046E-5	0.0
7	7.411101292431219E-4	2.7791629846617067E-5	7.411101292431218E-5	6.484713630877316E-5	1.8527753231078046E-5	0.0
8	7.411101292431219E-4	3.705550646215609E-5	7.411101292431218E-5	6.484713630877316E-5	1.8527753231078046E-5	0.0
9	7.411101292431219E-4	3.705550646215609E-5	8.337488953985121E-5	8.337488953985121E-5	2.7791629846617067E-5	0.0
10-14	7.485212305355529E-4	3.705550646215609E-5	9.634431680160583E-5	1.1116651938646827E-4	2.7791629846617067E-5	0.0
15-19	7.818711863514935E-4	3.89082817852639E-5	1.241359466482229E-4	1.5192757649483998E-4	4.26138324314795E-5	0.0
20-24	8.670988512144526E-4	5.5583259693234134E-5	1.4266369987930096E-4	1.9083585828010387E-4	8.337488953985121E-5	0.0
25-29	8.745099525068837E-4	6.484713630877316E-5	1.5378035181794778E-4	2.112163868342897E-4	1.3525259858686973E-4	0.0
30-34	9.097126836459319E-4	8.522766486295902E-5	1.630442284334868E-4	2.5938854523509263E-4	1.686025544028102E-4	0.0
35-39	9.449154147849803E-4	1.0190264277092926E-4	1.7786643101834926E-4	3.13119029605219E-4	2.315969153884756E-4	9.263876615539021E-6
40-44	0.00100420422512443	1.0560819341714487E-4	2.112163868342897E-4	3.612911880060219E-4	2.6865242185063166E-4	3.334995581594048E-5
45-49	0.0010671985861100955	1.241359466482229E-4	2.315969153884756E-4	4.1687444769925605E-4	2.982968270203565E-4	3.705550646215609E-5
50-54	0.0011079596432184671	1.3525259858686973E-4	2.501246686195536E-4	4.8172158400802917E-4	3.2794123219008145E-4	7.225823760120438E-5
55-59	0.0011691012288810247	1.4451647520240875E-4	2.6679964652752386E-4	5.150715398239698E-4	3.649967386522375E-4	1.333998232637619E-4
60-64	0.0012209789379280431	1.4822202584862437E-4	3.001496023434644E-4	5.428631696705867E-4	4.2057999834547164E-4	1.3895814923308534E-4
65-69	0.0012543288937439836	1.5563312714105557E-4	3.2794123219008145E-4	5.836242267789584E-4	4.354022009303341E-4	1.4822202584862437E-4
70-74	0.0015100118883328607	1.6489700375659462E-4	3.4646898542115944E-4	5.891825527482819E-4	4.5763550480762774E-4	1.593386777872712E-4
75-79	0.006506946934754609	1.8527753231078046E-4	3.631439633291297E-4	6.114158566255755E-4	4.872799099773526E-4	1.8713030763388826E-4
80-84	0.008595024723897105	2.130691621573975E-4	3.927883684988546E-4	6.299436098566536E-4	5.799186761327428E-4	1.945414089263195E-4
85-89	0.008622816353743722	2.5568299458887705E-4	4.150216723761482E-4	6.707046669650254E-4	7.448156798893376E-4	1.945414089263195E-4
90-94	0.008732130097807083	2.64946871204416E-4	4.4466607754587307E-4	6.966435214885345E-4	7.614906577973077E-4	1.945414089263195E-4
95-99	0.008780302256207885	2.7421074781995504E-4	4.891326853004605E-4	7.262879266582594E-4	7.689017590897389E-4	1.945414089263195E-4
100-104	0.008804388335408288	2.94591276374141E-4	5.243354164395087E-4	7.596378824741999E-4	7.78165635705278E-4	1.945414089263195E-4
105-109	0.00882662163928558	3.22382906220758E-4	5.558325969323414E-4	8.207794681367574E-4	7.78165635705278E-4	2.000997348956429E-4
110-114	0.00883588551590112	3.316467828362971E-4	5.650964735478804E-4	8.670988512144526E-4	7.874295123208169E-4	2.0380528554185851E-4
115-119	0.00886923547171706	3.4461621009805165E-4	5.743603501634194E-4	9.263876615539023E-4	7.966933889363561E-4	2.0380528554185851E-4
120-124	0.008922965956087187	3.70555064621561E-4	5.928881033944974E-4	9.337987628463336E-4	7.966933889363561E-4	2.0380528554185851E-4
125-129	0.009045249127412303	4.001994697912858E-4	6.132686319486833E-4	9.541792914005193E-4	8.189266928136495E-4	2.1306916215739757E-4
130-134	0.009134182342921477	4.2057999834547164E-4	6.280908345335458E-4	9.764125952778129E-4	8.874793797686385E-4	2.1306916215739757E-4
135-139	0.009262023840215915	4.2613832431479513E-4	6.373547111490847E-4	0.0010208792030324003	9.171237849383634E-4	2.2233303877293654E-4
140-144	0.009378748685571706	4.2613832431479513E-4	6.836740942267798E-4	0.0010486708328790175	9.449154147849803E-4	2.2233303877293654E-4
145-149	0.009467681901080882	4.2613832431479513E-4	7.151712747196125E-4	0.0010820207886949578	9.57884842046735E-4	2.2233303877293654E-4
150-154	0.00955290956594384	4.2613832431479513E-4	7.336990279506906E-4	0.0011172235198340062	0.001006057000447538	2.315969153884756E-4
155-159	0.009639990006129907	4.2613832431479513E-4	7.689017590897389E-4	0.00115613180161927	0.0010542291588483408	2.3530246603469118E-4
160-164	0.009710395468408003	4.335494256072263E-4	7.763128603821702E-4	0.0011802178808196714	0.0010968429912798202	2.501246686195536E-4
165-167	0.009828973089086904	4.3540220093033405E-4	7.96693388936356E-4	0.0012043039600200729	0.001133280905967607	2.5938854523509263E-4
>>END_MODULE
