##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293611
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10915975
Total Bases	1.8 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.36146216897712	39.0	39.0	39.0	37.0	39.0
2	40.14420773224563	41.0	40.0	41.0	38.0	41.0
3	40.18401013193966	41.0	40.0	41.0	38.0	41.0
4	40.19819823698754	41.0	40.0	41.0	38.0	41.0
5	40.19761890257169	41.0	40.0	41.0	38.0	41.0
6	40.18315853599884	41.0	40.0	41.0	38.0	41.0
7	40.205525296640936	41.0	40.0	41.0	38.0	41.0
8	40.1851431502912	41.0	40.0	41.0	38.0	41.0
9	40.16201612773939	41.0	40.0	41.0	38.0	41.0
10-14	40.09518957307982	41.0	40.0	41.0	38.0	41.0
15-19	39.965760200073746	41.0	40.0	41.0	38.0	41.0
20-24	39.78011017797311	41.0	40.0	41.0	37.6	41.0
25-29	39.53179973387627	40.2	39.0	41.0	36.6	41.0
30-34	39.41017543554286	40.2	39.0	41.0	35.6	41.0
35-39	39.261107212136345	40.6	38.4	41.0	35.0	41.0
40-44	38.73046682499731	40.0	36.8	41.0	35.0	41.0
45-49	38.062058386905434	39.0	35.4	41.0	35.0	41.0
50-54	37.35629978998669	37.2	35.0	40.2	34.4	41.0
55-59	36.66130257718619	36.2	35.0	39.0	34.0	41.0
60-64	35.91255588254828	35.0	34.8	37.4	33.6	40.0
65-69	35.53960803318073	35.0	35.0	36.4	33.6	39.0
70-74	35.311520977283294	35.0	35.0	36.0	34.0	37.8
75-79	35.12012380021024	35.0	35.0	35.2	33.2	37.0
80-84	35.03787804570824	35.0	35.0	35.0	33.0	36.6
85-89	34.836936215042634	35.0	34.6	35.0	32.6	36.2
90-94	39.649189152351866	40.6	39.8	40.6	37.8	40.6
95-99	39.914365221440164	41.0	40.0	41.0	38.0	41.0
100-104	39.78915030830332	41.0	40.0	41.0	37.0	41.0
105-109	39.63309820457527	41.0	39.4	41.0	36.4	41.0
110-114	39.44501328168527	40.6	39.0	41.0	35.8	41.0
115-119	39.161155100058394	40.0	38.2	41.0	35.0	41.0
120-124	38.90239100059081	40.0	38.0	41.0	35.0	41.0
125-129	38.41634088489455	39.6	36.8	40.6	34.6	41.0
130-134	38.257082577994325	39.0	35.8	41.0	34.6	41.0
135-139	37.837428314904535	38.6	35.0	41.0	34.8	41.0
140-144	37.180068024973764	36.8	35.0	40.0	34.0	41.0
145-149	36.50264327215757	35.6	35.0	38.8	34.0	41.0
150-154	35.865557334914016	35.0	35.0	37.0	34.0	39.4
155-159	35.34839029869854	35.0	35.0	36.0	34.0	38.0
160-164	34.98078039945298	35.0	35.0	35.2	33.4	36.6
165-169	34.74937479849639	35.0	35.0	35.0	33.0	36.0
170-174	34.61857135970972	35.0	35.0	35.0	33.0	36.0
175-178	34.29864647223876	35.0	34.5	35.0	32.25	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	51.0
30	644.0
31	4814.0
32	24325.0
33	89637.0
34	251283.0
35	603023.0
36	1594301.0
37	3463659.0
38	3730939.0
39	1141135.0
40	12161.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.405682955484966	26.915882456674733	29.25441840971604	22.424016178124262
2	22.923632566032808	27.82284679105623	28.022105217353467	21.231415425557497
3	20.086213095944245	26.541614468702978	30.472578033570063	22.899594401782707
4	21.34010017428585	25.298656327080266	30.692659153213526	22.66858434542036
5	22.576251777784396	26.271670647834938	28.067918807069454	23.084158767311212
6	22.483506970288957	26.832857348977075	27.913658651655027	22.76997702907894
7	22.650216769459437	26.979633060720644	27.382189863937946	22.987960305881977
8	22.888885326322203	27.13370083753398	26.940964961902164	23.03644887424165
9	22.884075861295027	27.319346187582877	27.16198965277953	22.634588298342567
10-14	22.881902899191324	27.20557897943152	26.993795790114945	22.91872233126221
15-19	22.826395260157707	27.202755594438425	27.15537366107929	22.81547548432458
20-24	22.793656086607015	27.038750088746085	27.214338618401012	22.953255206245892
25-29	22.861710474785806	26.980085608477484	27.023455073871094	23.134748842865616
30-34	22.90680768323489	27.049858578825987	27.05410373328997	22.98923000464915
35-39	22.890131206786386	27.051166753313378	27.05030929440568	23.008392745494564
40-44	22.930166109761153	27.017287965573388	26.98135897159896	23.07118695306649
45-49	23.013295651556547	26.97411087878087	26.98878661777807	23.02380685188451
50-54	23.012603088592638	27.03905239797636	26.94184623911286	23.006498274318144
55-59	22.98510210952297	26.944885821010033	26.95119400694853	23.118818062518464
60-64	22.961633752367515	27.030060072508412	27.001326038214636	23.006980136909437
65-69	22.933872604142095	27.02607508720018	26.98835788832422	23.0516944203335
70-74	22.90079447781806	27.003489839432575	26.98832674131262	23.107388941436746
75-79	22.87565517509888	27.009724738284945	27.05577651103085	23.05884357558532
80-84	22.891296471455824	27.031412219247482	26.99124723169483	23.086044077601862
85-89	22.85851332565346	26.972833851305083	26.9725865073894	23.19606631565206
90-94	21.756726279169637	26.673268017609107	29.05304354919939	22.516962154021865
95-99	22.668492066231245	27.144880860420383	27.307385019211587	22.87924205413678
100-104	22.825490335629947	27.253902825958686	27.059611680353747	22.86099515805762
105-109	22.732447261767607	27.190200459143632	27.17997873705092	22.89737354203784
110-114	22.75484313879586	27.02231924397908	27.264826252601654	22.958011364623403
115-119	22.855210717240144	27.095030170288364	27.009429269843217	23.040329842628275
120-124	22.807638478753596	27.07087840854277	27.038655861373755	23.082827251329878
125-129	22.796882705743364	27.08344528739745	27.10529162289601	23.014380383963168
130-134	22.92304367244209	27.10018622015872	26.985698783567468	22.991071323831722
135-139	22.948479518348286	27.033043398953538	26.962444624038266	23.05603245865991
140-144	22.9515696850858	27.102089492268668	27.01743092034582	22.92890990229971
145-149	22.90563436003572	27.137927634782606	27.01965007307738	22.936787932104295
150-154	22.86486074240501	27.19451535075701	27.004255787658444	22.936368119179537
155-159	22.812133130241445	27.21306939308208	27.11752751081495	22.85726996586153
160-164	22.782712682717975	27.332292347370913	27.081169216505973	22.803825753405143
165-169	22.697907435847846	27.294936338812665	27.144578658023853	22.862577567315633
170-174	22.68821610284023	27.392652392046564	27.20541407778514	22.713717427328074
175-178	22.628333077052844	27.442283892099447	27.145442412281163	22.78394061856655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	3.0
11	8.0
12	19.5
13	39.0
14	73.0
15	149.5
16	302.5
17	613.0
18	1253.5
19	2394.5
20	4288.5
21	7398.0
22	12154.5
23	18672.5
24	27280.5
25	38129.0
26	51037.5
27	65881.5
28	83008.0
29	102349.0
30	123150.5
31	145499.5
32	169107.0
33	193590.0
34	219260.0
35	245500.5
36	271004.0
37	297265.5
38	324459.0
39	350438.0
40	374957.0
41	397177.0
42	417264.5
43	432675.5
44	443294.0
45	448718.5
46	447168.0
47	440673.5
48	428514.5
49	411928.5
50	392235.5
51	369635.5
52	345700.5
53	320746.5
54	296774.5
55	273653.0
56	250625.5
57	228477.0
58	207324.0
59	187352.5
60	167871.5
61	149047.5
62	131608.0
63	115163.0
64	98689.0
65	83480.0
66	69627.5
67	56863.0
68	45546.0
69	35720.5
70	27414.5
71	20463.0
72	14948.5
73	10627.0
74	7360.0
75	4944.0
76	3228.0
77	2064.0
78	1251.0
79	751.0
80	428.5
81	223.5
82	120.0
83	54.5
84	28.5
85	15.0
86	3.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1110.0
92-93	2405.0
94-95	3950.0
96-97	5146.0
98-99	6309.0
100-101	7630.0
102-103	8497.0
104-105	10079.0
106-107	11161.0
108-109	12564.0
110-111	13246.0
112-113	14314.0
114-115	14974.0
116-117	15733.0
118-119	16524.0
120-121	17514.0
122-123	18383.0
124-125	19049.0
126-127	19573.0
128-129	20411.0
130-131	21391.0
132-133	23026.0
134-135	90098.0
136-137	100892.0
138-139	96142.0
140-141	93083.0
142-143	101958.0
144-145	101763.0
146-147	99510.0
148-149	102096.0
150-151	101682.0
152-153	95307.0
154-155	103967.0
156-157	99569.0
158-159	110484.0
160-161	105358.0
162-163	104390.0
164-165	106937.0
166-167	106924.0
168-169	108385.0
170-171	112917.0
172-173	115379.0
174-175	118028.0
176-177	334277.0
178-179	8123840.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.2535511965173
#Duplication Level	Percentage of total
1	87.46086502014849
2	10.30624107088547
3	1.452015128779665
4	0.3626459342150406
5	0.15467902834059752
6	0.09711726386816623
7	0.0575099209139929
8	0.03301289091299106
9	0.01969078089088145
>10	0.04176154895287448
>50	0.002707088109226384
>100	0.0
>500	0.0
>1k	0.011754323982575343
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.473439156832074E-4	0.0	0.0	0.0	0.0	0.0
2	7.51192632815667E-4	9.160885766044718E-6	0.0	9.160885766044718E-6	1.8321771532089437E-5	0.0
3	7.603535185817117E-4	1.8321771532089437E-5	0.0	2.7482657298134157E-5	1.8321771532089437E-5	0.0
4	7.603535185817117E-4	1.8321771532089437E-5	0.0	3.664354306417887E-5	2.7482657298134157E-5	0.0
5	7.603535185817117E-4	1.8321771532089437E-5	0.0	4.580442883022359E-5	2.7482657298134157E-5	0.0
6	7.603535185817117E-4	1.8321771532089437E-5	0.0	4.580442883022359E-5	3.664354306417887E-5	0.0
7	7.695144043477563E-4	1.8321771532089437E-5	0.0	4.580442883022359E-5	4.580442883022359E-5	0.0
8	7.695144043477563E-4	1.8321771532089437E-5	0.0	5.496531459626831E-5	4.580442883022359E-5	0.0
9	7.695144043477563E-4	1.8321771532089437E-5	0.0	7.328708612835775E-5	5.496531459626831E-5	0.0
10-14	7.786752901138011E-4	2.015394868529838E-5	2.015394868529838E-5	9.527321196686509E-5	6.0461846055895145E-5	0.0
15-19	7.933327073394727E-4	3.297918875776099E-5	4.580442883022359E-5	1.3374893218425288E-4	8.244797189440247E-5	0.0
20-24	8.043257702587264E-4	4.580442883022359E-5	6.779055466873092E-5	1.6306376663559598E-4	1.1909151495858134E-4	0.0
25-29	8.189831874843978E-4	5.313313744305936E-5	8.244797189440247E-5	2.0337166400619276E-4	1.4290981795029762E-4	0.0
30-34	8.281440732504427E-4	6.412620036231303E-5	8.611232620082036E-5	2.3818302991716268E-4	1.6489594378880495E-4	0.0
35-39	8.574589077017858E-4	6.412620036231303E-5	1.0626627488611873E-4	2.913161673602221E-4	1.758890067080586E-4	1.8321771532089437E-5
40-44	8.867737421531288E-4	7.145490897514881E-5	1.502385265631334E-4	3.389527733436546E-4	1.9054642393373018E-4	5.862966890268621E-5
45-49	9.289138166769346E-4	7.328708612835775E-5	1.8138553816768544E-4	3.884215564802961E-4	2.088681954658196E-4	9.893756627328296E-5
50-54	9.747182455071581E-4	7.695144043477563E-5	1.9787513254656595E-4	4.433868710765644E-4	2.27189966997909E-4	1.1909151495858134E-4
55-59	0.0010626627488611875	9.710538912007403E-5	2.216934355382822E-4	5.276670201241759E-4	2.858196359005952E-4	1.3741328649067078E-4
60-64	0.0011194602406106647	1.007697434264919E-4	2.5100826998962527E-4	5.954575747929067E-4	3.774284935610424E-4	1.6123158948238705E-4
65-69	0.00114511072075559	1.007697434264919E-4	2.803231044409684E-4	6.467585350827572E-4	4.7636605983432536E-4	1.6672812094201388E-4
70-74	0.0013887902821323795	1.1359498349895451E-4	2.8948399020701317E-4	7.145490897514881E-4	5.111774257452953E-4	2.0153948685298385E-4
75-79	0.005831819878664068	1.264202235714171E-4	3.3528841903723665E-4	7.896683530330547E-4	5.459887916562652E-4	2.180290812318643E-4
80-84	0.007735451940848161	1.2825240072462607E-4	3.59106722028953E-4	8.336406047100694E-4	6.321011178570857E-4	2.2902214415111794E-4
85-89	0.007775759838218757	1.465741722567155E-4	3.682676077949977E-4	8.941024507659646E-4	8.318084275568604E-4	2.2902214415111794E-4
90-94	0.007836221684274652	1.6489594378880495E-4	3.884215564802961E-4	9.179207537576807E-4	8.336406047100694E-4	2.2902214415111794E-4
95-99	0.007880193935951667	1.9237860108693911E-4	4.214007452380571E-4	9.417390567493971E-4	8.336406047100694E-4	2.2902214415111794E-4
100-104	0.007924166187628682	2.180290812318643E-4	4.305616310041018E-4	9.76550422660367E-4	8.336406047100694E-4	2.2902214415111794E-4
105-109	0.007953481022080025	2.308543213043269E-4	4.5254775684260907E-4	0.0010168583200309638	8.354727818632784E-4	2.2902214415111794E-4
110-114	0.007990124565144203	2.4551173852999846E-4	4.6903735122148956E-4	0.0010333479144098445	8.611232620082036E-4	2.2902214415111794E-4
115-119	0.008021271576748756	2.473439156832074E-4	5.2033831151134E-4	0.0010883132290061127	8.611232620082036E-4	2.2902214415111794E-4
120-124	0.00806707600557898	2.674978643685058E-4	5.5514967742231E-4	0.0011377820121427542	8.721163249274572E-4	2.3451867561074478E-4
125-129	0.008112880434409202	2.7482657298134156E-4	5.899610433332798E-4	0.001209236921117903	8.959346279191736E-4	2.3818302991716268E-4
130-134	0.008209985823529277	2.93148344513431E-4	5.991219290993247E-4	0.0012367195784160371	9.289138166769344E-4	2.473439156832074E-4
135-139	0.008347399110019948	2.949805216666399E-4	6.082828148653693E-4	0.0012587057042545442	9.527321196686507E-4	2.693300415217147E-4
140-144	0.008422518373301515	3.1147011604552047E-4	6.266045863974588E-4	0.0012825240072462606	9.76550422660367E-4	2.876518130538042E-4
145-149	0.008504966345195918	3.151344703519384E-4	6.595837751552198E-4	0.0012916848930123054	0.0010113617885713368	2.93148344513431E-4
150-154	0.008565428191251813	3.279597104244009E-4	6.724090152276823E-4	0.0013081744873911858	0.0010535018630951428	3.1147011604552047E-4
155-159	0.008657037048912259	3.3345624188402777E-4	6.962273182193986E-4	0.001337489321842529	0.001073655811780441	3.279597104244009E-4
160-164	0.008737652843653453	3.462814819564904E-4	7.127169125982791E-4	0.0013869581049791705	0.001106635000538202	3.481136591096993E-4
165-167	0.0088494156499992	3.5727454487574406E-4	7.237099755175329E-4	0.0014626880939784734	0.001129842577812182	3.481136591096993E-4
>>END_MODULE
